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Brazilian Oral Research 2022The aim of this scoping review was to provide sufficient information about the effectiveness of ozone gas in virus inactivation of surfaces and objects under different... (Review)
Review
The aim of this scoping review was to provide sufficient information about the effectiveness of ozone gas in virus inactivation of surfaces and objects under different environmental conditions. The review was performed according to the list of PRISMA SrC recommendations and the JBI Manual for Evidence Synthesis for Scoping Reviews. The review was registered in Open Science Framework (OSF). EMBASE (Ovid), Lilacs, LIVIVO, MEDLINE (PubMed), SciELO, Scopus and Web of Science were primary sources, and "gray literature" was searched in OpenGray and OpenThesis. A study was included if it reported primary data on the effect of ozone gas application for vehicle-borne and airborne virus inactivation. No language or publication date restriction was applied. The search was conduct on July 1, 2020. A total of 16,120 studies were screened, and after exclusion of noneligible studies, fifteen studies fulfilled all selection criteria. Application of ozone gas varied in terms of concentration, ozone exposure period and the devices used to generate ozone gas. Twelve studies showed positive results for inactivation of different virus types, including bacteriophages, SARS-CoV-2 surrogates and other vehicle-borne viruses. Most of the studies were classified as unclear regarding sponsorship status. Although most of the population has not yet been vaccinated against COVID-19, disinfection of environments, surfaces, and objects is an essential prevention strategy to control the spread of this disease. The results of this Scoping Review demonstrate that ozone gas is promising for viral disinfection of surfaces.
Topics: COVID-19; Delivery of Health Care; Disinfection; Humans; Ozone; SARS-CoV-2
PubMed: 35081224
DOI: 10.1590/1807-3107bor-2022.vol36.0006 -
Journal of Microbiological Methods Mar 2022Ozone treatment is an eco-friendly and cost-effective approach to achieve material disinfection, and this disinfection method is of utmost importance in the present...
Ozone treatment is an eco-friendly and cost-effective approach to achieve material disinfection, and this disinfection method is of utmost importance in the present global pandemic. The efficacy of ozone's oxidative potential on common microorganisms has been extensively studied, particularly in the food and water treatment industries. However, little is still understood regarding its antimicrobial capabilities for the treatment of textile substrates in air. In this study, fabric swatches inoculated with bacterial and fungal suspensions are exposed to ozone for different durations and at different ozone concentrations. Pathogenic bacteria (Escherichia coli, Staphylococcus aureus), and fungi (Aspergillus fumigatus, and Candida albicans), are the microbes utilised in this study. The efficacy of ozone is demonstrated by the complete removal of microbiota on the tested swatches when a concentration and exposure duration of 20 ppm and 4 mins are respectively maintained in a test ozone chamber. We expect the insights from this work to guide the development of new ozonation techniques capable of rapid sterilisation in industrial & public settings.
Topics: Bacteria; Disinfection; Escherichia coli; Ozone; Water Purification
PubMed: 35131364
DOI: 10.1016/j.mimet.2022.106431 -
The Review of Scientific Instruments Aug 2020A portable device for cleaning and ozone sterilization of small-sized delicate dental instruments that cannot withstand the high heat and humidity of standard...
A portable device for cleaning and ozone sterilization of small-sized delicate dental instruments that cannot withstand the high heat and humidity of standard autoclaving has been developed. The device contains a remote unit for magnetic mechanical washing, an ultrasonic bath for pre-cleaning treatment, and a container for ozone sterilization with a reactor based on dielectric barrier discharge. The maximum ozone concentration in water reached 8.5 mg/l for 10 min of operation at a water temperature of 19 °C. The results of inactivation of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus are presented. Distilled water with such seeded micro-organisms was bubbled with an ozone-air mixture at an ozone concentration of 5.8 mg/l at 17 °C for 5 min, 10 min, 20 min, and 30 min. The maximum bactericidal effect was manifested for Pseudomonas aeruginosa cells with their total elimination within 10 min. Inactivation of Escherichia coli bacteria was monitored after 20 min of treatment, and Staphylococcus aureus-after 30 min of treatment.
Topics: Dental Instruments; Electric Impedance; Mechanical Phenomena; Microbial Viability; Ozone; Sterilization; Temperature; Time Factors; Ultrasonic Waves; Water
PubMed: 32872939
DOI: 10.1063/1.5145279 -
Chemosphere Sep 2020The problem of bacterial antibiotic resistance has attracted considerable research attention, and the effects of water treatment on antibiotic resistant bacteria (ARB)... (Review)
Review
The problem of bacterial antibiotic resistance has attracted considerable research attention, and the effects of water treatment on antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are being increasingly investigated. As an indispensable part of the water treatment process, disinfection plays an important role in controlling antibiotic resistance. At present, there were many studies on the effects of conventional and new sterilization methods on ARB and ARGs. However, there is a lack of literature relating to the limitations of conventional methods and analysis of new techniques. Therefore, this review focuses on analyzing the deficiencies of conventional disinfection and the development of new methods for antibiotic resistance control to guide future research. Firstly, we analyzed the effects and drawbacks of conventional disinfection methods, such as chlorine (Cl), ultraviolet (UV) and ozone on antibiotic resistance control. Secondly, we discuss the research progress and shortcomings of new sterilization methods in antibiotic resistance. Finally, we propose suggestions for future research directions. There is an urgent need for new effective and low-cost sterilization methods. Disinfection via UV and chlorine in combination, UV/chlorine showed greater potential for controlling ARGs.
Topics: Chlorine; Disinfection; Drug Resistance, Bacterial; Sterilization; Ultraviolet Rays; Water Purification
PubMed: 32957272
DOI: 10.1016/j.chemosphere.2020.126831 -
The Journal of Hand Surgery May 2020Health care is an important contributor to environmental waste. In 2013, the health care sector was responsible for substantial fractions of national air pollution... (Review)
Review
Health care is an important contributor to environmental waste. In 2013, the health care sector was responsible for substantial fractions of national air pollution emissions and impacts, including acid rain (12%), greenhouse gas emissions (10%), smog formation (10%), air pollutants (9%), stratospheric ozone depletion (1%), and carcinogenic and noncarcinogenic air toxins (1% to 2%). Operating rooms produce between 20% and 70% of total hospital waste. Hand surgery, with short, high-volume cases, is a notable contributor to this environmental and subsequent financial burden. This article aims to highlight the Lean and Green initiative proposed by the American Association for Hand Surgery along with the American Society for Surgery of the Hand, the American Society for Peripheral Nerve Surgery, and the American Society of Reconstructive Microsurgery in 2015, to reduce the amount of waste generated by hand surgery. We have reviewed the literature to propose multiple ways to reduce both material and nonmaterial waste-energy consumption, sterilization techniques, reprocessing of devices, patient transportation, production of surgical supply, anesthesia, and sanitation in hand surgery.
Topics: Air Pollutants; Hand; Humans; Operating Rooms; United States
PubMed: 31928797
DOI: 10.1016/j.jhsa.2019.10.031 -
Human Reproduction Update Jan 2023Air pollution is both a sensory blight and a threat to human health. Inhaled environmental pollutants can be naturally occurring or human-made, and include...
BACKGROUND
Air pollution is both a sensory blight and a threat to human health. Inhaled environmental pollutants can be naturally occurring or human-made, and include traffic-related air pollution (TRAP), ozone, particulate matter (PM) and volatile organic compounds, among other substances, including those from secondhand smoking. Studies of air pollution on reproductive and endocrine systems have reported associations of TRAP, secondhand smoke (SHS), organic solvents and biomass fueled-cooking with adverse birth outcomes. While some evidence suggests that air pollution contributes to infertility, the extant literature is mixed, and varying effects of pollutants have been reported.
OBJECTIVE AND RATIONALE
Although some reviews have studied the association between common outdoor air pollutants and time to pregnancy (TTP), there are no comprehensive reviews that also include exposure to indoor inhaled pollutants, such as airborne occupational toxicants and SHS. The current systematic review summarizes the strength of evidence for associations of outdoor air pollution, SHS and indoor inhaled air pollution with couple fecundability and identifies gaps and limitations in the literature to inform policy decisions and future research.
SEARCH METHODS
We performed an electronic search of six databases for original research articles in English published since 1990 on TTP or fecundability and a number of chemicals in the context of air pollution, inhalation and aerosolization. Standardized forms for screening, data extraction and study quality were developed using DistillerSR software and completed in duplicate. We used the Newcastle-Ottawa Scale to assess risk of bias and devised additional quality metrics based on specific methodological features of both air pollution and fecundability studies.
OUTCOMES
The search returned 5200 articles, 4994 of which were excluded at the level of title and abstract screening. After full-text screening, 35 papers remained for data extraction and synthesis. An additional 3 papers were identified independently that fit criteria, and 5 papers involving multiple routes of exposure were removed, yielding 33 articles from 28 studies for analysis. There were 8 papers that examined outdoor air quality, while 6 papers examined SHS exposure and 19 papers examined indoor air quality. The results indicated an association between outdoor air pollution and reduced fecundability, including TRAP and specifically nitrogen oxides and PM with a diameter of ≤2.5 µm, as well as exposure to SHS and formaldehyde. However, exposure windows differed greatly between studies as did the method of exposure assessment. There was little evidence that exposure to volatile solvents is associated with reduced fecundability.
WIDER IMPLICATIONS
The evidence suggests that exposure to outdoor air pollutants, SHS and some occupational inhaled pollutants may reduce fecundability. Future studies of SHS should use indoor air monitors and biomarkers to improve exposure assessment. Air monitors that capture real-time exposure can provide valuable insight about the role of indoor air pollution and are helpful in assessing the short-term acute effects of pollutants on TTP.
Topics: Pregnancy; Female; Humans; Air Pollution; Air Pollutants; Tobacco Smoke Pollution; Particulate Matter; Fertility; Environmental Pollutants
PubMed: 35894871
DOI: 10.1093/humupd/dmac029 -
JBRA Assisted Reproduction Apr 2021Busulfan is one of the most common chemotherapeutic drugs and has the ability to induce apoptosis in testicular germ cells, which leads to infertility. In this study,...
OBJECTIVE
Busulfan is one of the most common chemotherapeutic drugs and has the ability to induce apoptosis in testicular germ cells, which leads to infertility. In this study, the effects of ozone therapy and melatonin were evaluated on testicular disorders induced by busulfan.
METHODS
In this study, we divided 24 male mice into four groups: control group, groups treated with busulfan, busulfan/melatonin, and busulfan/ozone. At the end of a 35-day period, blood samples were taken from the mice and their testosterone levels were measured. Both of the mice's testes were removed and weighed, afterwards, each one of them was used for evaluation of morphology by Johnson's score, as well as for measuring the diameter and thickness of seminiferous tubules. The other testis was homogenized for measuring Malondialdehyde (MDA) and antioxidant status using Catalase (CAT), Super Oxide Dismutase (SOD), and Total Antioxidant Capacity (TAC) levels. Epididymis spermatozoa were also used to evaluate motility, morphology, and sperm count.
RESULTS
Busulfan significantly reduced the testis quality (weight, sperm parameters, testosterone, CAT, SOD, and TAC levels) and increased MDA and destruction of seminiferous tubules compared to the control group. Ozone and melatonin treatments significantly increased testis quality, sperm parameters, MDA, and antioxidant status, but they did not affect the TAC level.
CONCLUSIONS
This study showed that similar to melatonin, ozone can reduce the effect of busulfan toxicity on mice testis. However, further studies are needed to understand the precise mechanism of ozone function on testis.
Topics: Animals; Busulfan; Male; Melatonin; Mice; Oxidative Stress; Ozone; Spermatozoa; Testis
PubMed: 33507719
DOI: 10.5935/1518-0557.20200081 -
Micromachines Mar 2024Since ozone is highly corrosive, it can substantially affect the mechanical and chemical properties of the materials; consequently, it could affect the applicability of...
Since ozone is highly corrosive, it can substantially affect the mechanical and chemical properties of the materials; consequently, it could affect the applicability of those materials in medical applications. The effect of ozone sterilization on the chemical and mechanical properties of additively manufactured specimens of biocompatible poly(methyl-methacrylate) was observed. FDM 3D-printed specimens of biocompatible PMMA in groups of five were exposed to high concentrations of ozone generated by corona discharge for different durations and at different ozone concentrations inside an enclosed chamber with embedded and calibrated ozone, temperature, and humidity sensors. A novel approach using laser-induced fluorescence (LIF) and spark-discharge optical emission spectrometry (SD-OES) was used to determine an eventual change in the chemical composition of specimens. Mechanical properties were determined by testing the tensile strength and Young's modulus. A calibrated digital microscope was used to observe the eventual degradation of material on the surface of the specimens. SD-OES and LIF analysis results do not show any detectable sterilization-caused chemical degradation, and no substantial difference in mechanical properties was detected. There was no detectable surface degradation observed under the digital microscope. The results obtained suggest that ozone sterilization appears to be a suitable technique for sterilizing PMMA medical devices.
PubMed: 38675283
DOI: 10.3390/mi15040472 -
International Journal of Molecular... Oct 2023The best form of prevention against human infection through bacteria, viruses, and other parasites is ozone disinfection of wastewater and drinking water as a highly... (Review)
Review
The best form of prevention against human infection through bacteria, viruses, and other parasites is ozone disinfection of wastewater and drinking water as a highly effective, well-known method. Various preclinical studies showed promising results, which are being revisited and reconsidered in times of pandemics and led to interesting results in recent clinical trials and reports, as presented by the example of protective measures against COVID-19 in particularly vulnerable clinical personnel. The application of ozone in the form of the low-dose concept induces its regulation by interference of ozone or its peroxides into the redox equilibrium of the biological system, which finally results in the restoration of the glutathione equilibrium. The antioxidant system is activated, the immune system is modulated, and thus the defense mechanisms are improved. In patients with rheumatoid arthritis, repeated ozone treatments have led to new findings in "immunomodulation" through ozone. The more effective immune response is discussed as the response of innate immune memory and opens interesting aspects for complementary treatment of autoimmune diseases.
Topics: Humans; Ozone; Drinking Water; Disinfection; Wastewater; Oxidation-Reduction
PubMed: 37958730
DOI: 10.3390/ijms242115747 -
The Science of the Total Environment Mar 2023The large-scale global COVID-19 has a profound impact on human society. Timely and effectively blocking the virus spread is the key to controlling the pandemic growth.... (Review)
Review
The large-scale global COVID-19 has a profound impact on human society. Timely and effectively blocking the virus spread is the key to controlling the pandemic growth. Ozone-based inactivation and disinfection techniques have been shown to effectively kill SARS-CoV-2 in water, aerosols and on solid surface. However, the lack of an unified information and discussion on ozone-based inactivation and disinfection in current and previous pandemics and the absence of consensus on the main mechanisms by which ozone-based inactivation of pandemic causing viruses have hindered the possibility of establishing a common basis for identifying best practices in the utilization of ozone technology. This article reviews the research status of ozone (O) disinfection on pandemic viruses (especially SARS-CoV-2). Taking sterilization kinetics as the starting point while followed by distinguishing the pandemic viruses by enveloped and non-enveloped viruses, this review focuses on analyzing the scope of application of the sterilization model and the influencing factors from the experimental studies and data induction. It is expected that the review could provide an useful reference for the safe and effective O utilization of SARS-CoV-2 inactivation in the post-pandemic era.
Topics: Humans; Disinfection; Ozone; Pandemics; COVID-19; SARS-CoV-2; Viruses
PubMed: 36496014
DOI: 10.1016/j.scitotenv.2022.160711