-
PloS One 2022In this study, we sequenced a bacteria isolate Pandoraea sp. 892iso isolated from a Phytophthora rubi strain which is an important plant pathogenic oomycete, identified...
In this study, we sequenced a bacteria isolate Pandoraea sp. 892iso isolated from a Phytophthora rubi strain which is an important plant pathogenic oomycete, identified through genome and combined the data with existing genomic data from other 28 the genus of Pandoraea species. Next, we conducted a comparative genomic analysis of the genome structure, evolutionary relationships, and pathogenic characteristics of Pandoraea species. Our results identified Pandoraea sp. 892iso as Pandoraea sputorum at both the genome and gene levels. At the genome level, we carried out phylogenetic analysis of single-copy, gene co-linearity, ANI (average nucleotide identity) and AAI (average amino acid identity) indices, rpoB similarity, MLSA phylogenetic analysis, and genome-to-genome distance calculator calculations to identify the relationship between Pandoraea sp. 892iso and P. sputorum. At the gene level, the quorum sensing genes ppnI and ppnR and the OXA-159 gene were assessed. It is speculated that Pandoraea sp. 892iso is the endosymbiont of the Oomycetes strain of Phytophthora rubi.
Topics: Burkholderiaceae; Phylogeny; Quorum Sensing; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 35930552
DOI: 10.1371/journal.pone.0272435 -
Emerging Infectious Diseases Nov 2023Pandoraea spp. are gram-negative, nonfermenting rods mainly known to infect patients with cystic fibrosis (CF). Outbreaks have been reported from several CF centers. We... (Review)
Review
Pandoraea spp. are gram-negative, nonfermenting rods mainly known to infect patients with cystic fibrosis (CF). Outbreaks have been reported from several CF centers. We report a Pandoraea spp. outbreak comprising 24 non-CF patients at a large university hospital and a neighboring heart center in Germany during July 2019-December 2021. Common features in the patients were critical illness, invasive ventilation, antimicrobial pretreatment, and preceding surgery. Complicated and relapsing clinical courses were observed in cases with intraabdominal infections but not those with lower respiratory tract infections. Genomic analysis of 15 isolates identified Pandoraea commovens as the genetically most similar species and confirmed the clonality of the outbreak strain, designated P. commovens strain LB-19-202-79. The strain exhibited resistance to most antimicrobial drugs except ampicillin/sulbactam, imipenem, and trimethoprim/sulfamethoxazole. Our findings suggest Pandoraea spp. can spread among non-CF patients and underscore that clinicians and microbiologists should be vigilant in detecting and assessing unusual pathogens.
Topics: Humans; Cystic Fibrosis; Gram-Negative Bacteria; Anti-Infective Agents; Trimethoprim, Sulfamethoxazole Drug Combination; Burkholderiaceae; Germany
PubMed: 37877517
DOI: 10.3201/eid2911.230493 -
Microorganisms May 2023The respiratory tract of lung transplant recipients (LTR) is likely to be colonized with non-fermentative Gram-negative rods. As a consequence of the improvements in... (Review)
Review
The respiratory tract of lung transplant recipients (LTR) is likely to be colonized with non-fermentative Gram-negative rods. As a consequence of the improvements in molecular sequencing and taxonomy, an increasing number of bacterial species have been described. We performed a review of the literature of bacterial infections in LTR involving non-fermentative Gram-negative rods with exclusion of , , spp. and spp. Overall, non-fermenting GNR were recovered from 17 LTR involving the following genera: , , , , , and . We then discuss the issues raised by these bacteria, including detection and identification, antimicrobial resistance, pathogenesis, and cross-transmission.
PubMed: 37374970
DOI: 10.3390/microorganisms11061468 -
Microbiology Spectrum May 2024Lovebugs appeared in large numbers across a wide area in Seoul, South Korea, in June 2023. The sudden appearance of exotic insects not only discomforts people but also...
Lovebugs appeared in large numbers across a wide area in Seoul, South Korea, in June 2023. The sudden appearance of exotic insects not only discomforts people but also fosters anxiety, as their potential for pathogen transmission would be unknown. In this study, targeted next-generation sequencing (NGS) of the 16S rRNA gene V4 region was performed using iSeq 100 to screen for bacteria in lovebugs. Forty-one lovebugs (20 females and 21 males) collected in Seoul, Korea, were identified as based on mitochondrial cytochrome oxidase subunit 1 sequencing data using PCR. We analyzed the microbiome of the lovebugs and detected 453 species of bacteria. Among all bacteria screened based on NGS, was detected in all samples with an average relative abundance of 80.40%, followed by and . Diversity (alpha and beta) between females and males did not differ; however, only showed a higher relative abundance in females. Sequencing analysis of using a gene-specific primer by PCR showed that it had higher sequence similarity to the symbiont of arthropods than to the spotted fever group rickettsiae. Eleven samples in which was detected by iSeq 100 were confirmed by PCR and exhibited 100% sequence identity to strain DSM 23570. Consequently, the likelihood of pathogen transmission to humans is low. The applied method may play a crucial role in swiftly identifying bacterial species in the event of future outbreaks of exotic insects that may be harmful to humans.IMPORTANCELovebugs have recently emerged in large numbers in Seoul, causing major concern regarding potential health risks. By performing the next-generation sequencing of the 16S rRNA gene V4 region, we comprehensively examined the microbiome of these insects. We identified the presence of numerous bacteria, including and . Reassuringly, subsequent tests confirmed that these detected bacteria were not pathogenic. The present study addresses health concerns related to lovebugs and shows the accuracy and efficiency of our detection technique. Such methods prove invaluable for rapidly identifying bacterial species during potential outbreaks of unfamiliar insects, thereby ensuring public safety.
PubMed: 38809007
DOI: 10.1128/spectrum.03809-23 -
Journal of Natural Products Jan 2022Single-strain cultivation of a mountain soil-derived sp. GA02 and its coculture with sp. GA02N produced two aromatic products, gwanakosides A and B ( and ,...
Single-strain cultivation of a mountain soil-derived sp. GA02 and its coculture with sp. GA02N produced two aromatic products, gwanakosides A and B ( and , respectively). Their spectroscopic analysis revealed that is a new dichlorinated naphthalene glycoside and is a pentacyclic aromatic glycoside. The assignment of the two chlorine atoms in was confirmed by the analysis of its band-selective CLIP-HSQMBC spectrum. The sugars in the gwanakosides were identified as 6-deoxy-α-l-talopyranose based on H-H coupling constants, Rotating frame Overhauser enhancement spectroscopy (ROESY) NMR correlations, and chemical derivatization followed by spectroscopic and chromatographic analyses. The absolute configuration of , whose production was enhanced approximately 100-fold in coculture, was proposed based on a quantum mechanics-based chemical shift analysis method, DP4 calculations, and the chemically determined configuration of 6-deoxy-α-l-talopyranose. Gwanakoside A displayed inhibitory activity against pathogenic bacteria, including (MIC = 8 μg/mL) and (MIC = 15 μg/mL), and antiproliferative activity against several human cancer cell lines (IC = 5.6-19.4 μM).
Topics: Humans; Burkholderiaceae; Carbon-13 Magnetic Resonance Spectroscopy; Cell Line, Tumor; Cell Proliferation; Coculture Techniques; Drug Screening Assays, Antitumor; Microbial Sensitivity Tests; Mycobacterium; Proton Magnetic Resonance Spectroscopy; Quantum Theory; Spectrometry, Mass, Electrospray Ionization; Staphylococcus aureus; Streptomyces
PubMed: 34931849
DOI: 10.1021/acs.jnatprod.1c00703 -
Environmental Research Oct 2021This research was aimed to evaluate the phytochemical profile, bactericidal activity of Hygrophila spinosa against multidrug resistant Pandoraea sputorum and assess...
This research was aimed to evaluate the phytochemical profile, bactericidal activity of Hygrophila spinosa against multidrug resistant Pandoraea sputorum and assess their antioxidant competence against various radicals and studied their hepatoprotective and nephroprotective activity on HepG2 and HEK 293 cell line. The results showed that the methanol extract has various phytochemical components with reasonable quantity. Fortunately, the multidrug-resistant P. sputorum was sensitive (22.8 ± 0.2 mm of the zone of inhibition) at 15 mg mL concentration of methanol extract. The higher concentration of phenolic and other phytochemical components, showed significant antioxidant activity against ferric, DPPH, hydroxyl, and ABTS radicals, with IC values of 71.09, 64.333, 91.157, and 104.931 g mL, respectively. Surprisingly, the methanol extract possesses hepato and nephroprotective activity against CCl and cisplatin-induced cytotoxicity on HepG2 and HEK 293 cell lines, respectively. It maintains the cell viability as up to 90.48% and 90.35% of HepG2 and EK 293 cell line at the concentration of 20 μg mL. The FTIR analysis states that the methanol extract possesses a significant functional group responsible for these multi-potential activities. These results suggest that, the methanol extract of H. spinosa might contain the most significant bioactive components with outstanding medicinal properties.
Topics: Acanthaceae; Anti-Bacterial Agents; Burkholderiaceae; Drug Resistance, Multiple, Bacterial; HEK293 Cells; Hep G2 Cells; Humans; Kidney; Liver; Methanol; Plant Extracts; Protective Agents
PubMed: 34186080
DOI: 10.1016/j.envres.2021.111594 -
BMC Infectious Diseases Oct 2019Pandoraea species is a newly described genus, which is multidrug resistant and difficult to identify. Clinical isolates are mostly cultured from cystic fibrosis (CF)... (Review)
Review
BACKGROUND
Pandoraea species is a newly described genus, which is multidrug resistant and difficult to identify. Clinical isolates are mostly cultured from cystic fibrosis (CF) patients. CF is a rare disease in China, which makes Pandoraea a total stranger to Chinese physicians. Pandoraea genus is reported as an emerging pathogen in CF patients in most cases. However, there are few pieces of evidence that confirm Pandoraea can be more virulent in non-CF patients. The pathogenicity of Pandoraea genus is poorly understood, as well as its treatment. The incidence of Pandoraea induced infection in non-CF patients may be underestimated and it's important to identify and understand these organisms.
CASE PRESENTATION
We report a 44-years-old man who suffered from pneumonia and died eventually. Before his condition deteriorated, a Gram-negative bacilli was cultured from his sputum and identified as Pandoraea Apista by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS).
CONCLUSION
Pandoraea spp. is an emerging opportunistic pathogen. The incidences of Pandoraea related infection in non-CF patients may be underestimated due to the difficulty of identification. All strains of Pandoraea show multi-drug resistance and highly variable susceptibility. To better treatment, species-level identification and antibiotic susceptibility test are necessary.
Topics: Adult; Burkholderiaceae; China; Cystic Fibrosis; Gram-Negative Bacterial Infections; Humans; Intracranial Hemorrhage, Traumatic; Male; Pneumonia, Bacterial; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sputum
PubMed: 31640582
DOI: 10.1186/s12879-019-4420-6 -
Italian Journal of Pediatrics Sep 2019Pandoraea species are gram negative, motile, non-spore forming, rod shaped and oxidase positive, obligate aerobes bacteria, and have one polar flagellum. Most of...
BACKGROUND
Pandoraea species are gram negative, motile, non-spore forming, rod shaped and oxidase positive, obligate aerobes bacteria, and have one polar flagellum. Most of Pandoraea species are associated with lung infections in cystic fibrosis patients. Cystic fibrosis is the most prevalent autosomal recessive hereditary disease in the world that affects various organs of the body. The main important cause of death in these patients is lung involvement. This study was conducted to isolate and identify Pandoraea bacterium from bronchoalveolar lavage and sputum samples of cystic fibrosis patients in Shiraz, Iran.
METHODS
In this research 31 samples of bronchoalveolar lavage and sputum were examined by culture and PCR method. Then confirmed isolates were evaluated for susceptibility to different antibiotics and ability to produce biofilm.
RESULTS
The results of this study after cultivation, purification and DNA extraction led to the isolation of 4 Pandoraea bacterium by PCR using specific primers. Antibiotic susceptibility test were indicated all isolates were resistant to gentamicin, amikacin and imipenem and susceptible to ciprofloxacin, trimethoprim-sulfumethoxazole, piperacillin and tetracycline. Ability to create biofilm was indicated by some of Pandoraea isolates. According to findings of this study, ability to synthesis biofilm by Pandoraea isolates and resistance to some antibiotics are very important.
CONCLUSIONS
Our study notes the role of P. pnomenusa as an emerging pathogen that can cause chronic lung colonization in CF patients. Identification tools need to be accurate and must be based on molecular techniques. Also our findings should raise awareness about antibiotic resistance in cystic fibrosis patients in Iran and ability of including bacterial agents to produce biofilm is an alarm for public health. Thus clinicians should exercise caution about finding of clinical relevance of this pathogen to the infection and prescribing antibiotics, especially in cases of children infections.
Topics: Bronchoalveolar Lavage Fluid; Burkholderiaceae; Child; Cystic Fibrosis; Female; Gram-Negative Bacterial Infections; Humans; Iran; Male; Respiratory Tract Infections; Sputum
PubMed: 31477148
DOI: 10.1186/s13052-019-0687-x -
Journal of Hazardous Materials Sep 2023The treatment of chlorinated volatile organic compounds faces challenges of secondary pollution and less-efficiency due to the substitution of chlorine. Microbial fuel...
The treatment of chlorinated volatile organic compounds faces challenges of secondary pollution and less-efficiency due to the substitution of chlorine. Microbial fuel cells (MFCs) provide a promising opportunity for its abatement. In this study, a novel FeO nanoparticles and silicone-based powder (SP) were integrated and immobilized on carbon felt (CF+FeO@SP), which was further used as anode in the chlorobenzene (CB) powered MFC. Owing to the cooperation between SP and FeO, the anode exhibited excellent performance for both biodechlorination and power generation. The results indicated that the CF+FeO@SP anode loaded MFC achieved 98.5% removal of 200 mg/L CB within 28 h, and the maximum power density was 675.9 mW/m, which was a 45.6% increase compared to that of the bare CF anode. Microbial community analysis indicated that the genera Comamonadaceae, Pandoraea, Obscuribacteraceae, and Truepera were dominated, especially, the Comamonadaceae and Obscuribacteraceae showed outstanding affinity for FeO and SP, respectively. Moreover, the proportion of live bacteria, secretion of extracellular polymer substances, and protein content in the extracellular polymer substances were significantly increased by modifying FeO@SP onto the carbon-based anode. Thus, this study provides new insights into the development of MFCs for refractory and hydrophobic volatile organic compounds removal.
Topics: Bacteria; Bioelectric Energy Sources; Carbon; Electricity; Electrodes; Polymers; Powders; Volatile Organic Compounds; Water Purification; Water Pollutants, Chemical
PubMed: 37315409
DOI: 10.1016/j.jhazmat.2023.131794 -
Frontiers in Microbiology 2019Comparative analysis of partial , , and gene sequences of 84 reference strains and field isolates revealed several clusters that included no taxonomic reference...
Comparative analysis of partial , , and gene sequences of 84 reference strains and field isolates revealed several clusters that included no taxonomic reference strains. The , , and phylogenetic trees were used to select 27 strains for whole-genome sequence analysis and for a comparative genomics study that also included 41 publicly available genome sequences. The phylogenomic analyses included a Genome BLAST Distance Phylogeny approach to calculate pairwise digital DNA-DNA hybridization values and their confidence intervals, average nucleotide identity analyses using the OrthoANIu algorithm, and a whole-genome phylogeny reconstruction based on 107 single-copy core genes using bcgTree. These analyses, along with subsequent chemotaxonomic and traditional phenotypic analyses, revealed the presence of 17 novel species among the strains analyzed, and allowed the identification of several unclassified strains reported in the literature. The genus has an open pan genome that includes many orthogroups in the 'Xenobiotics biodegradation and metabolism' KEGG pathway, which likely explains the enrichment of these species in polluted soils and participation in the biodegradation of complex organic substances. We propose to formally classify the 17 novel species as sp. nov. (type strain LMG 31117 = CCUG 73385), sp. nov. (type strain LMG 31108 = CCUG 73386), sp. nov. (type strain LMG 31011 = CCUG 73384), sp. nov. (type strain LMG 20603 = ATCC BAA-110), sp. nov. (type strain LMG 20602 = ATCC BAA-109), sp. nov. (type strain LMG 31118 = CCUG 73387), sp. nov. (type strain LMG 31106 = CCUG 39680), sp. nov. (type strain LMG 31010 = CCUG 73378), sp. nov. (type strain LMG 31110 = CCUG 73383), sp. nov. (type strain LMG 31012 = CCUG 73380), sp. nov. (type strain LMG 31112 = CCUG 73379), sp. nov. (type strain LMG 31009 = CCUG 73377), sp. nov. (type strain LMG 31116 = CCUG 73389), sp. nov. (type strain LMG 31109 = CCUG 73390), sp. nov. (type strain LMG 31114 = CCUG 73388), sp. nov. (type strain LMG 31014 = CCUG 73382), and sp. nov. (type strain LMG 31013 = CCUG 73381).
PubMed: 31781066
DOI: 10.3389/fmicb.2019.02556