-
Journal of Laboratory Physicians Jun 2021is a novel gram-negative bacillus usually isolated from respiratory specimens of cystic fibrosis patients. Few cases of bacteremia have also been reported due to this...
is a novel gram-negative bacillus usually isolated from respiratory specimens of cystic fibrosis patients. Few cases of bacteremia have also been reported due to this rare pathogen. Emergence of multidrug-resistant isolates of this bacillus is of grave concern. Here, we report a very interesting and unusual case of bacteremia in a coronavirus disease (COVID)-positive elderly diabetic man suffering from pneumonia. Prompt isolation and antibiotic sensitivity testing guided the patient's treatment and yielded favorable outcome. The need of automated methods for identification and sensitivity testing limits the reporting of this rare but important pathogen in hospital settings. Detailed research work and studies are needed in this direction to better understand this pathogen and its clinical manifestations for better patient outcome.
PubMed: 34483568
DOI: 10.1055/s-0041-1730847 -
Environmental Research Nov 2023The widespread presence of oxytetracycline (OTC) in aquatic ecosystems poses both health risks and ecological concerns. The present study revealed the beneficial role of...
The widespread presence of oxytetracycline (OTC) in aquatic ecosystems poses both health risks and ecological concerns. The present study revealed the beneficial role of hydrogen peroxide (HO)-pretreated biochar (BC) derived from agricultural hardwood waste in an activated sludge (AS) bioprocess. The BC addition significantly enhanced the removal and detoxification of OTC and its byproducts. BC was initially modified using HO to improve its OTC adsorption. Two AS reactors were then established, one with HO-modified BC and one without, and both were exposed to OTC. The BC-added reactor exhibited significantly higher OTC removal rates during both the start-up (0.97 d) and steady-state (0.98 d) phases than the reactor without BC (0.54 d and 0.83 d, respectively). Two novel transformation pathways for OTC were proposed, with four byproducts originating from OTC identified, some of which were found to be more toxic than OTC itself. The BC-added reactor had significantly higher system functioning in terms of its heterotrophic activity and the reduction of the toxicity of OTC and its byproducts, as illustrated by structure-based toxicity simulations, antimicrobial susceptibility experiments, analytical chemistry, and bioinformatics analysis. Bioinformatics revealed two novel bacterial populations closely related to the known OTC-degrader Pandoraea. The ecophysiology and selective enrichment of these populations suggested their role in the enzymatic breakdown and detoxification of OTC (e.g., via demethylation and hydrogenation). Overall, the present study highlighted the beneficial role of HO-modified BC in combination with the AS microbiome in terms of enhancing treatment performance and resilience, reducing the toxicological disruption to biodiversity, and detoxifying micropollutants.
PubMed: 37543124
DOI: 10.1016/j.envres.2023.116832 -
Antimicrobial Agents and Chemotherapy Dec 2019We tested the activities of ceftazidime-avibactam, ceftolozane-tazobactam, meropenem-vaborbactam, piperacillin-tazobactam, and 11 other antimicrobial agents against 420...
We tested the activities of ceftazidime-avibactam, ceftolozane-tazobactam, meropenem-vaborbactam, piperacillin-tazobactam, and 11 other antimicrobial agents against 420 , , , and strains, 89% of which were cultured from respiratory specimens from persons with cystic fibrosis. Among the β-lactam-β-lactamase inhibitor agents, meropenem-vaborbactam had the greatest activity against and , including multidrug-resistant and extensively-drug-resistant strains. None of the newer β-lactam-β-lactamase combination drugs showed increased activity compared to that of the older agents against or spp.
Topics: Achromobacter; Anti-Bacterial Agents; Boronic Acids; Burkholderia; Cystic Fibrosis; Humans; Meropenem; Microbial Sensitivity Tests; Piperacillin; Pseudomonas aeruginosa; Stenotrophomonas; Stenotrophomonas maltophilia; Tazobactam; beta-Lactamase Inhibitors
PubMed: 31611364
DOI: 10.1128/AAC.01595-19 -
Current Eye Research May 2020: This study aimed to evaluate the microbial contamination level and its influencing factors of rigid gas permeable (RGP) trial lenses and lens cases in China.: A total...
: This study aimed to evaluate the microbial contamination level and its influencing factors of rigid gas permeable (RGP) trial lenses and lens cases in China.: A total of 107 RGP trial lenses and lens cases were collected from 7 main hospitals or optometric centers in China. Three sites including the lenses, case interiors and case screw tops were sampled for bacterial and fungal culture and identification. The contamination rates of these three sites and their relationship with lens care regimes were further analyzed.: The overall contamination rate was 73.8% for either lenses or cases, and 43.0% of lenses, 57.0% of case interiors and 65.4% of case screw tops respectively. The most frequently isolated microorganisms were spp., spp., spp., and spp. from all three sites. The contamination rate was positively related to the lens use frequency. Compared with dry-stored lenses, the contamination rate was significantly higher in wet-stored group ( < .001*). Inadequate disinfection and improper lens and case care regimes were also associated with higher contamination rates.: Our study reported that the RGP trial lenses and cases used for fittings had a considerably high contamination rate. The safe use of RGP trial lenses and education of optometrists on the regular maintenance of trial lenses should be emphasized.
Topics: Adult; Bacteria; Bacteriological Techniques; China; Contact Lens Solutions; Contact Lenses; Equipment Contamination; Equipment and Supplies; Female; Fungi; Humans; Male; Pyrrolidines; Young Adult
PubMed: 31697186
DOI: 10.1080/02713683.2019.1687726 -
Infection and Drug Resistance 2021Bacteremia by spp. has rarely been described before. We report the first case of a possible prosthetic valve endocarditis, according to the modified Duke criteria, in...
Bacteremia by spp. has rarely been described before. We report the first case of a possible prosthetic valve endocarditis, according to the modified Duke criteria, in a 37-year old male injecting drug user suffering from recurrent endocarditis. Furthermore, we demonstrate biofilm formation by the isolates of this patient and investigate antibiotic resistance.
PubMed: 33854344
DOI: 10.2147/IDR.S301138 -
Microbiology Resource Announcements Dec 2023We report here seven draft genomes of bacterial strains from two Danish wastewater facilities, two of which might be characterized as a new group within the and...
We report here seven draft genomes of bacterial strains from two Danish wastewater facilities, two of which might be characterized as a new group within the and genera, respectively. These genomes will provide useful references for understanding bacterial interactions and horizontal gene transfer within bacterial communities.
PubMed: 37966239
DOI: 10.1128/MRA.00529-23 -
Proceedings of the National Academy of... Nov 2019Despite the omnipresence of specific host-symbiont associations with acquisition of the microbial symbiont from the environment, little is known about how the...
Despite the omnipresence of specific host-symbiont associations with acquisition of the microbial symbiont from the environment, little is known about how the specificity of the interaction evolved and is maintained. The bean bug acquires a specific bacterial symbiont of the genus from environmental soil and harbors it in midgut crypts. The genus consists of over 100 species, showing ecologically diverse lifestyles, and including serious human pathogens, plant pathogens, and nodule-forming plant mutualists, as well as insect mutualists. Through infection tests of 34 species and 18 taxonomically diverse bacterial species, we demonstrate here that nonsymbiotic and even its outgroup could stably colonize the gut symbiotic organ and provide beneficial effects to the bean bug when inoculated on aposymbiotic hosts. However, coinoculation revealed that the native symbiont always outcompeted the nonnative bacteria inside the gut symbiotic organ, explaining the predominance of the native symbiont in natural bean bug populations. Hence, the abilities for colonization and cooperation, usually thought of as specific traits of mutualists, are not unique to the native symbiont but, to the contrary, competitiveness inside the gut is a derived trait of the native symbiont lineage only and was thus critical in the evolution of the insect gut symbiont.
Topics: Animals; Burkholderia; Heteroptera; Host-Pathogen Interactions; Intestines; Models, Biological; Symbiosis
PubMed: 31636183
DOI: 10.1073/pnas.1912397116 -
Animal Bioscience Jan 2021Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on...
OBJECTIVE
Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood.
METHODS
A total of 300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium, and low temperature (HT, MT, and LT), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis.
RESULTS
Our data showed that compared to the MT group, there was a decrease of muscle leucine and tyrosine (p<0.05), as well as an increase of methionine in the HT group (p<0.05) and a decrease of serine in the LT group. Examination of microbiota shift revealed that at genus level, the relative abundance of Turicibacter and Parabacteroides was increased in the HT group (p<0.05) and that the relative abundances of Pandoraea, Achromobacter, Prevotella, Brevundimonas, and Stenotrophomonas in the LT group were higher than those in the MT group (p<0.05). In addition, there were substantial correlations between microbes and amino acids. In the HT group. Turicibacter was negatively correlated with aspartic acid and tyrosine, whereas Parabacteroides was positively correlated with methionine (p<0.05). In the LT group, there were multiple positive correlations between Achromobacter and arginine, isoleucine or tyrosine; between Prevotella and cysteine or phenylalanine; between Brevundimonas and cysteine; and between Stenotrophomonas and cysteine as well as a negative correlation between Stenotrophomonas and serine.
CONCLUSION
Our findings demonstrated that amino acid content of breast muscle and intestinal microbiota profile was affected by different ambient temperatures. Under heat exposure, augmented abundance of Parabacteroides was correlated with elevated methionine. Low temperature treatment may affect muscle tyrosine content through the regulation of Achromobacter.
PubMed: 32898964
DOI: 10.5713/ajas.20.0314 -
Chemosphere Sep 2023Bioremediation has tremendous potential to mitigate the serious threats posed by polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs). In the present study,...
Bioremediation has tremendous potential to mitigate the serious threats posed by polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs). In the present study, nine bacterial-fungal consortia were progressively acclimated under different culture conditions. Among them, a microbial consortium 1, originating from activated sludge and copper mine sludge microorganisms, was developed through the acclimation of a multi-substrate intermediate (catechol)-target contaminant (Cd, phenanthrene (PHE)). Consortium 1 exhibited the best PHE degradation, with an efficiency of 95.6% after 7 d of inoculation, and its tolerance concentration for Cd was up to 1800 mg/L within 48 h. Bacteria Pandoraea and Burkholderia-Caballeronia-Paraburkholderia, as well as fungi Ascomycota and Basidiomycota predominated in the consortium 1. Furthermore, a biochar-loaded consortium was constructed to better cope with the co-contamination behavior, which exhibited excellent adaptation to Cd ranging of 50-200 mg/L. Immobilized consortium efficiently degraded 92.02-97.77% of 50 mg/L PHE within 7 d while removing 93.67-99.04% of Cd. In remediation of co-pollution, immobilization technology improved the bioavailability of PHE and dehydrogenase activity of the consortium to enhance PHE degradation, and the phthalic acid pathway was the main metabolic pathway. As for Cd removal, oxygen-containing functional groups (-OH, C=O, and C-O) of biochar or microbial cell walls and EPS components, fulvic acid and aromatic proteins, participated through chemical complexation and precipitation. Furthermore, immobilization led to more active consortium metabolic activity during the reaction, and the community structure developed in a more favorable direction. The dominant species were Proteobacteria, Bacteroidota, and Fusarium, and the predictive expression of functional genes corresponding to key enzymes was elevated. This study provides a basis for combining biochar and acclimated bacterial-fungal consortia for co-contaminated site remediation.
Topics: Cadmium; Sewage; Soil Pollutants; Phenanthrenes; Polycyclic Aromatic Hydrocarbons; Bacteria; Biodegradation, Environmental; Acclimatization; Soil
PubMed: 37327827
DOI: 10.1016/j.chemosphere.2023.139234 -
Microbiology Resource Announcements Mar 2020is a newly identified Gram-negative bacterial species that was isolated from the respiratory tract of an Australian cystic fibrosis patient. The complete assembled...
is a newly identified Gram-negative bacterial species that was isolated from the respiratory tract of an Australian cystic fibrosis patient. The complete assembled genome sequences of two consecutive isolates (second isolate collected 11 months after antibiotic treatment) from the same individual are presented here.
PubMed: 32217674
DOI: 10.1128/MRA.00060-20