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Parasites, Hosts and Diseases Feb 2023Parasite infection rates estimated by examining ancient coprolites can provide insights into parasitism in Joseon society. Using newly discovered Joseon period cases is...
Updates on parasite infection prevalence in the Joseon period based on parasitological studies of human coprolites isolated from archaeological sites in the cities of Euijeongbu, Gumi, and Wonju.
Parasite infection rates estimated by examining ancient coprolites can provide insights into parasitism in Joseon society. Using newly discovered Joseon period cases is essential to regularly update the parasite infection rates and reinforce the reliability of our previous estimations. In the present study, we investigated parasite infections in Joseon coprolites newly isolated from the cities of Euijeongbu, Gumi, and Wonju. We then updated the overall parasite infection rates of Joseon period samples (n= 30) as follows: 86.7% (26/30) for Trichuris trichiura, 56.7% (17/30) for Ascaris lumbricoides, 30.0% (9/30) for Clonorchis sinensis, and 30.0% (9/30) for Paragonimus westermani. The parasite infection rates in the Joseon society, estimated through coprolite examination, were very similar to those determined previously despite the addition of new cases to the existing data pool.
Topics: Animals; Humans; Cities; Prevalence; Reproducibility of Results; Ascaris lumbricoides; Parasitic Diseases
PubMed: 37170469
DOI: 10.3347/PHD.22129 -
Infection and Drug Resistance 2023The infection is a parasitic foodborne infection that induces systemic symptoms with eosinophilia in humans. Here, we described pneumothorax in addition to pulmonary...
The infection is a parasitic foodborne infection that induces systemic symptoms with eosinophilia in humans. Here, we described pneumothorax in addition to pulmonary opacities with eosinophilia in a man with a positive serology. He was misdiagnosed with chronic eosinophilic pneumonia (CEP) during the initial phase. Paragonimiasis can share similar clinical findings with CEP in cases where the worm is confined to the lungs. The findings of the current study suggest that paragonimiasis and CEP can be distinguished from each other by the presence of various symptoms. Notably, eosinophilia with pneumothorax should be an important diagnostic factor for paragonimiasis.
PubMed: 37138842
DOI: 10.2147/IDR.S402392 -
Zhongguo Xue Xi Chong Bing Fang Zhi Za... Mar 2020To establish a rapid nucleic acid detection technique for identification of based on the recombinase aided isothermal amplification assay (RAA) and assess its...
OBJECTIVE
To establish a rapid nucleic acid detection technique for identification of based on the recombinase aided isothermal amplification assay (RAA) and assess its diagnostic efficiency.
METHODS
The mitochondrial gene sequence of (GenBank accession number: AB018440) was used as a target sequence. The primers were designed according to the RAA reaction principle and synthesized, and RAA was performed using the generated primers. genomic DNA at various concentrations and the pMD19-T (Simple) vector containing various copies of the target gene fragment were amplified using RAA to evaluate its sensitivity for detection of , and RAA was em- ployed to detect the genomic DNA of G1 genotype, , , , , , , , , , and to evaluate its specificity. In addition, the optimized RAA was employed to detect nine tissue specimens of -infected animals, 3 fecal samples from -infected dogs and 2 fecal samples from field infected dogs to examine its reliability and feasibility.
RESULTS
The established RAA was able to detect the specific target gene fragment of within 40 min. The lowest detect limit of RAA was 10 pg if genomic DNA served as a template. If the re- combinant plasmid was used as a template, the minimally detectable copy number of RAA was 104. In addition, RAA was nega- tive for the genomic DNA of G1 genotype, , , , , , , , , , and . The established RAA was positive for detection of the tissue specimens of infected animals, and simulated and field dog stool samples.
CONCLUSIONS
A rapid, sensitive and specific RAA is established, which shows promising values in identification of and gene diagnosis of alveolar echinococcosis.
Topics: Animals; DNA Primers; Dog Diseases; Dogs; Echinococcosis; Echinococcus multilocularis; Feces; Nucleic Acid Amplification Techniques; Recombinases; Reproducibility of Results; Sensitivity and Specificity
PubMed: 32458606
DOI: 10.16250/j.32.1374.2019284 -
International Journal of Infectious... Aug 2020Next-generation sequencing (NGS) is an emerging method with the potential of pan-pathogen screening. This study described a case of eosinophilic meningitis (EoM) with...
Next-generation sequencing (NGS) is an emerging method with the potential of pan-pathogen screening. This study described a case of eosinophilic meningitis (EoM) with enzyme-linked immunosorbent assay (ELISA)-negative results for Angiostrongylus cantonensis (A. cantonensis), Trichinella spiralis and Paragonimus westermani and a positive identification of A. cantonensis by NGS in the cerebrospinal fluid.
Topics: Angiostrongylus cantonensis; Animals; Enzyme-Linked Immunosorbent Assay; Eosinophilia; High-Throughput Nucleotide Sequencing; Humans; Male; Meningitis; Strongylida Infections
PubMed: 32502661
DOI: 10.1016/j.ijid.2020.05.108