-
IEEE Transactions on Nanobioscience Jan 2023pH-sensitive pectin beads were proposed as a protective capsule for layered zinc hydroxide-drug (LZH-Drug) nanohybrids in the gastrointestinal tract in this paper....
pH-sensitive pectin beads were proposed as a protective capsule for layered zinc hydroxide-drug (LZH-Drug) nanohybrids in the gastrointestinal tract in this paper. Baclofen was intercalated between LZH layers using the co-precipitation method as a model drug. By combining LZH-baclofen with pectin, the resulting nanohybrid (LZH-baclofen) was used to make bio-nanocomposite hydrogel beads. FTIR, XRD, and SEM analyses were used to characterize the produced products. Baclofen anions are vertical to the LZH layers in the shape of a monolayer, according to the interlayer space of 19.6Å. The presence of nanocomposites is demonstrated by FTIR, which exhibits a peak at 3489 cm-1 for the OH group, 1564 and 1384 cm-1 for the-COO- vibration mode, indicating that baclofen is intercalated between the layered structures. After intercalation, baclofen's thermal stability is greatly improved. The nanohybrid is more compact, with agglomerates and flat surfaces of the intercalated substance, shown by SEM. In vitro release behaviors of baclofen from LZH and bio-nanocomposites in buffer solution were examined under pH values (pH = 1.2, 6.8, 7.4) chosen from a model of the passing materials through the gastrointestinal tract. For pectin encapsulated LZH-baclofen nanohybrid, drug release studies indicated superior protection against stomach pH and regulated release under intestinal tract conditions. Furthermore, nanohybrid and nanocomposite treatment of a normal fibroblast cell line resulted in cell survival up to 12.5 g/mL for a 24-h period, with inhibition reducing dose-dependently at higher concentrations. A novel intercalation molecule with a sustained release mode and improved toxicity against normal fibroblast cell lines has been produced as a result of the strong host-guest contacts between the LZH lattice and the baclofen anion. Further study into the utilization of brucite-like host materials in drug delivery systems should be based on these findings.
Topics: Baclofen; Delayed-Action Preparations; Pectins; Hydroxides; Nanocomposites
PubMed: 35294354
DOI: 10.1109/TNB.2022.3155785 -
Frontiers in Nutrition 2021The purpose of this study is to quantify the effect of adding sodium alginate and pectin to a carbohydrate (CHO) beverage on exogenous glucose (ExGluc) oxidation rate...
PURPOSE
The purpose of this study is to quantify the effect of adding sodium alginate and pectin to a carbohydrate (CHO) beverage on exogenous glucose (ExGluc) oxidation rate compared with an isocaloric CHO beverage.
METHODS
Following familiarization, eight well-trained endurance athletes performed four bouts of prolonged running (105 min; 71 ± 4% of VOmax) while ingesting 175 mL of one of the experimental beverages every 15 min. In randomized order, participants consumed either 70 gh of maltodextrin and fructose (10% CHO; NORM), 70 gh of maltodextrin, fructose, sodium alginate, and pectin (10% CHO; ENCAP), 180 gh of maltodextrin, fructose, sodium alginate, and pectin (26% CHO; HiENCAP), or water (WAT). All CHO beverages had a maltodextrin:fructose ratio of 1:0.7 and contained 1.5 gL of sodium chloride. Total substrate oxidation, ExGluc oxidation rate, blood glucose, blood lactate, serum non-esterified fatty acid (NEFA) concentration, and RPE were measured for every 15 min. Every 30 min participants provided information regarding their gastrointestinal discomfort (GID).
RESULTS
There was no significant difference in peak ExGluc oxidation between NORM and ENCAP (0.63 ± 0.07 and 0.64 ± 0.11 gmin, respectively; > 0.5), both of which were significantly lower than HiENCAP (1.13 ± 0.13 gmin, < 0.01). Both NORM and HiENCAP demonstrated higher total CHO oxidation than WAT from 60 and 75 min, respectively, until the end of exercise, with no differences between CHO trials. During the first 60 min, blood glucose was significantly lower in WAT compared with NORM and HiENCAP, but no differences were found between CHO beverages. Both ENCAP and HiENCAP demonstrated a higher blood glucose concentration from 60-105 min than WAT, and ENCAP was significantly higher than HiENCAP. There were no significant differences in reported GID symptoms between the trials.
CONCLUSIONS
At moderate ingestion rates (i.e., 70 gh), the addition of sodium alginate and pectin did not influence the ExGluc oxidation rate compared with an isocaloric CHO beverage. At very high ingestion rates (i.e., 180 gh), high rates of ExGluc oxidation were achieved in line with the literature.
PubMed: 35127792
DOI: 10.3389/fnut.2021.810041 -
International Journal of Biological... Oct 2019Several studies have been conducted on polysaccharides derived from natural sources, and their different biological properties have been reported. Their low toxicity and...
Several studies have been conducted on polysaccharides derived from natural sources, and their different biological properties have been reported. Their low toxicity and antiviral effects i.e., their action on several steps of viral replication, have been extensively examined. In this work, pectin isolated from Inga spp. fruit pulp was first characterized and evaluated using HEp-2 cells against the herpes simplex virus type 1 (HSV-1) and the poliovirus (PV). The isolated pectin (denoted as PDTS) was characterized by infrared spectroscopy, NMR and Gel permeation chromatography. The cytotoxicity was analyzed by the MTT method and antiviral activity by plaque reduction assay, immunofluorescence assay (IF) and polymerase chain reaction (PCR). The cytotoxic concentration (CC) of PDTS was 870 μg.mL and the inhibitory concentrations (IC) were 179 μg.mL and 58 μg.mL for HSV-1 and PV, respectively. Greater inhibitory effect was observed when the cells were simultaneously treated with PDTS and infected, suggesting that PDTS inhibited the initial viral replication stages, revealing its antiviral potential.
Topics: Antiviral Agents; Cell Line; Fabaceae; Herpesvirus 1, Human; Pectins; Poliovirus; Virus Replication
PubMed: 31376449
DOI: 10.1016/j.ijbiomac.2019.07.212 -
European Journal of Pharmacology Nov 2019Myocardial ischemia/reperfusion (IR) injury is caused by the restoration of the coronary blood flow following an ischemic episode. Accumulating evidence suggests that...
Myocardial ischemia/reperfusion (IR) injury is caused by the restoration of the coronary blood flow following an ischemic episode. Accumulating evidence suggests that galectin-3, a β-galactoside-binding lectin, acts as a biomarker in heart disease. However, it remains unclear whether manipulating galectin-3 affects the susceptibility of the heart to IR injury. In this study, RNA sequencing (RNA-seq) analysis identified that Lgals3 (galecin-3) plays an indispensable role in IR-induced cardiac damage. Immunostaining and immunoblot assays confirmed that the expression of galectin-3 was markedly increased in myocardial IR injury both in vivo and in vitro. Echocardiographic analysis showed that cardiac dysfunction in experimental IR injury was significantly attenuated by galectin-3 inhibitors including pectin (1%, i.p.) from citrus and binding peptide G3-C12 (5.0 mg/kg, i.p.). Galectin-3 inhibitor-treated mice exhibited smaller infarct sizes and decreased tissue injury. Furthermore, TUNEL staining showed that galectin-3 inhibition suppressed IR-mediated cardiomyocyte apoptosis. Mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (mPTP) levels were well-preserved and IR-induced changes of mitochondrial cyto c, cytosol cyto c, caspase-9, caspase-3, Bcl-2 and Bax in the galectin-3 inhibitor-treated groups were observed. Our findings indicate that the pathological upregulation of galectin-3 contributes to IR-induced cardiac dysfunction and that galectin-3 inhibition ameliorates myocardial injury, highlighting its therapeutic potential.
Topics: Animals; Apoptosis; Cardiotonic Agents; Cell Line; Galectin 3; Heart; Homeostasis; Male; Mice; Mice, Inbred C57BL; Mitochondria; Myocardial Infarction; Myocardial Reperfusion Injury; Pectins; Peptides; Up-Regulation
PubMed: 31568784
DOI: 10.1016/j.ejphar.2019.172701 -
International Journal of Biological... Aug 2021Biomaterials for effective hemorrhage control are urgently needed in clinics as uncontrolled bleeding is associated with high mortality. Herein, we developed an...
Biomaterials for effective hemorrhage control are urgently needed in clinics as uncontrolled bleeding is associated with high mortality. Herein, we developed an injectable and in situ photo-crosslinkable hybrid hemostatic hydrogel by combining pectin methacrylate (PECMA) and gelatin methacryloyl (GelMA). This modular material system combines ionic- and photo-crosslinking chemistries to design interpenetrating networks (IPN) exhibiting tunable rheology, highly porous structure, and controllable swelling and mechanical properties. By simply changing the calcium (0-15 mM) and polymer (1.5-7%) content used for the sequential crosslinking of hydrogels via calcium gelation and UV-photopolymerization, it was possible to precisely modulate the injectability, degradation, and swelling ratio. Moreover, it is demonstrated that PECMA/GelMA hydrogels present good cytocompatibility and uniquely synergize the hemostatic properties of calcium ions on PECMA, the amine residues on GelMA, and the highly porous network toward rapid blood absorption and fast coagulation effect. An in vitro porcine skin bleeding model confirmed that the hydrogel could be directly injected into the wound and rapidly photo-crosslinked, circumventing the bleeding and decreasing the coagulation time by 39%. Importantly, the crosslinked hydrogel could be easily removed to prevent secondary wound injury. Overall, this injectable hybrid PECMA/GelMA hydrogel stands as a promising hemostatic material.
Topics: Animals; Calcium; Cell Line; Gelatin; Hemostasis; Injections; Methacrylates; Mice; Pectins; Physical Phenomena; Porosity; Swine; Wound Healing
PubMed: 34197849
DOI: 10.1016/j.ijbiomac.2021.06.162 -
Journal of Food Biochemistry Jul 2021Tomato is usually harvested at an early ripening stage with high firmness suitable for storage and transportation but lacks many quality parameters such as sugars,...
Tomato is usually harvested at an early ripening stage with high firmness suitable for storage and transportation but lacks many quality parameters such as sugars, organic acids, and phenolics. In a recent study, we have selected introgression lines (ILs) IL4-2 and IL5-1, developed from a cross between the Solanum pennellii and the Solanum lycopersicum M82, that exhibit differentiated postharvest shelf-life characteristics in the fruit compared to M82 and the rest of the ILs. Here, we first structurally and biochemically characterized IL4-2, IL5-1, and their parent M82 to decipher the cell wall mechanistic difference between soft (IL4-2) and firm (IL5-1) lines at two postharvest ripening periods. Generally, IL4-2 had more active cell wall modifications in terms of ripening-related gene expression, water-soluble pectin, and cell wall structure under the microscope, which probably makes this line softer than IL5-1. We also evaluated these lines based on commercial quality parameters, sugars, phenolics, organic, and amino acids to gain insight into their commercial and functional quality and reveal noticeable differences. In summary, the contribution of the S. pennellii IL5-1 and IL4-2 to the shelf life of the tomato was structurally characterized, and the component differences meeting the quality criteria were revealed.
PubMed: 34251032
DOI: 10.1111/jfbc.13858 -
Pharmaceutics May 2024A general procedure to prepare gold nanourchins (GNUs) via a seed-mediated method was followed using dopamine hydrochloride as a reducing agent and silver nitrate salt...
A general procedure to prepare gold nanourchins (GNUs) via a seed-mediated method was followed using dopamine hydrochloride as a reducing agent and silver nitrate salt (AgNO) as a shape-directing agent. The novelty of this study comes from the successful incorporation of the prepared gold urchins as an aqueous suspension in a nasal pressurized metered dose inhaler (pMDI) formulation and the investigation of their potential for olfactory targeting for direct nose-to-brain drug delivery (NTBDD). The developed pMDI formulation was composed of 0.025% / GNUs, 2% / Milli-Q water, and 2% / EtOH, with the balance of the formulation being HFA134a propellant. Particle integrity and aerosolization performance were examined using an aerosol exposure system, whereas the nasal deposition profile was tested in a sectioned anatomical replica of human nasal airways. The compatibility of the gold dispersion with the nasal epithelial cell line RPMI 2650 was also investigated in this study. Colloidal gold was found to be stable following six-month storage at 4 °C and during the lyophilization process utilizing a pectin matrix for complete re-dispersibility in water. The GNUs were intact and discrete following atomization via a pMDI, and 13% of the delivered particles were detected beyond the nasal valve, the narrowest region in the nasal cavity, out of which 5.6% was recovered from the olfactory region. Moreover, the formulation was found to be compatible with the human nasal epithelium cell line RPMI 2650 and excellent cell viability was observed. The formulated GNU-HFA-based pMDI is a promising approach for intranasal drug delivery, including deposition in the olfactory region, which could be employed for NTBDD applications.
PubMed: 38794331
DOI: 10.3390/pharmaceutics16050669 -
Pharmaceutics Aug 2023The aim of this project is to use pectin- and chitosan-modified solid lipid nanoparticles for bovine lactoferrin to enhance its cellular uptake and transport.
AIM
The aim of this project is to use pectin- and chitosan-modified solid lipid nanoparticles for bovine lactoferrin to enhance its cellular uptake and transport.
METHODS
Solid lipid particles containing bovine lactoferrin (bLf) were formulated through the solvent evaporation technique, incorporating stearic acid along with either chitosan or pectin modification. bLf cellular uptake and transport were evaluated in vitro using the human adenocarcinoma cell line Caco-2 cell model.
RESULTS AND DISCUSSION
The bLf-loaded SLPs showed no significant effect on cytotoxicity and did not induce apoptosis within the eight-hour investigation. The use of confocal laser scanning microscopy confirmed that bLf follows the receptor-mediated endocytosis, whereas the primary mechanism for the cellular uptake of SLPs was endocytosis. The bLf-loaded SLPs had significantly more cellular uptake compared to bLf alone, and it was observed that this impact varied based on the time, temperature, and concentration. Verapamil and EDTA were determined to raise the apparent permeability coefficients (App) of bLf and bLf-loaded SLPs.
CONCLUSION
This occurred because they hindered efflux by interacting with P-glycoproteins and had a penetration-enhancing influence. These findings propose the possibility of an additional absorption mechanism for SLPs, potentially involving active transportation facilitated by the P-glycoprotein transporter in Caco-2 cells. These results suggest that SLPs have the potential to be applied as effective carriers to improve the oral bioavailability of proteins and peptides.
PubMed: 37631382
DOI: 10.3390/pharmaceutics15082168 -
Pediatric and Developmental Pathology :... 2021Absent submucosal ganglion cells in biopsies 1-3 cm above the pectinate line establishes the pathologic diagnosis of Hirschsprung Disease (HD). Calretinin stains both...
BACKGROUND
Absent submucosal ganglion cells in biopsies 1-3 cm above the pectinate line establishes the pathologic diagnosis of Hirschsprung Disease (HD). Calretinin stains both ganglion cells and their mucosal neurites and has gained importance in HD diagnosis. Absent calretinin positive mucosal neurites in biopsies at the appropriate level above the pectinate line is highly specific for HD. Whether this applies to lower biopsies is uncertain. To address this, we studied anorectal canal autopsy specimens from infants.
METHODS
We performed an autopsy study of infant anorectal canal specimens to describe calretinin staining in this region. Calretinin staining was correlated with histologic and gross landmarks.
RESULTS
In all 15 non-HD specimens, calretinin positive mucosal neurites were present in glandular mucosa up to the anorectal line where neurites rapidly diminished. Age range was preterm 26 weeks to 3 months.
CONCLUSIONS
Calretinin positive mucosal neurites are present in glandular mucosa up to the anorectal line in young infants. This is potentially important regarding neonatal HD biopsy level and diagnosis. Positive calretinin staining at the anorectal line favors normal innervation making HD unlikely. Absent calretinin positive neurites in glandular mucosa is worrisome for HD in young infants, regardless of location.
Topics: Autopsy; Biopsy; Calbindin 2; Hirschsprung Disease; Humans; Immunohistochemistry; Infant; Infant, Newborn; Rectum
PubMed: 34266330
DOI: 10.1177/10935266211030172 -
ACS Applied Bio Materials Aug 2023Natural polymers from organic wastes have gained increasing attention in the biomedical field as resourceful second raw materials for the design of biomedical devices...
Natural polymers from organic wastes have gained increasing attention in the biomedical field as resourceful second raw materials for the design of biomedical devices which can perform a specific bioactive function and eventually degrade without liberating toxic residues in the surroundings. In this context, patches and bandages, that need to support the skin wound healing process for a short amount of time to be then discarded, certainly constitute good candidates in our quest for a more environmentally friendly management. Here, we propose a plant-based microfibrous scaffold, loaded with vitamin C (VitC), a bioactive molecule which acts as a protecting agent against UV damages and as a wound healing promoter. Fibers were fabricated via electrospinning from various zein/pectin formulations, and subsequently cross-linked in the presence of Ca to confer them a hydrogel-like behavior, which we exploited to tune both the drug release profile and the scaffold degradation. A comprehensive characterization of the physico-chemical properties of the zein/pectin/VitC scaffolds, either pristine or cross-linked, has been carried out, together with the bioactivity assessment with two representative skin cell populations (human dermal fibroblast cells and skin keratinocytes, HaCaT cells). Interestingly, col-1a gene expression of dermal fibroblasts increased after 3 days of growth in the presence of the microfiber extraction media, indicating that the released VitC was able to stimulate collagen mRNA production overtime. Antioxidant activity was analyzed on HaCaT cells via DCFH-DA assay, highlighting a fluorescence intensity decrease proportional to the amount of loaded VitC (down to 50 and 30%), confirming the protective effect of the matrices against oxidative stress. Finally, the most performing samples were selected for the in vivo test on a skin UVB-burn mouse model, where our constructs demonstrated to significantly reduce the inflammatory cytokines expression in the injured area (50% lower than the control), thus constituting a promising, environmentally sustainable alternative to skin patches.
Topics: Animals; Humans; Male; Mice; Antioxidants; Ascorbic Acid; Biocompatible Materials; Burns; Cell Line; Hydrogels; Keratinocytes; Mice, Inbred C57BL; Wound Healing; Zein; Zea mays
PubMed: 37493659
DOI: 10.1021/acsabm.3c00214