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Biometals : An International Journal on... Oct 2023Bacillus toyonensis SFC 500-1E is a member of the consortium SFC 500-1 able to remove Cr(VI) and simultaneously tolerate high phenol concentrations. In order to... (Review)
Review
Bacillus toyonensis SFC 500-1E is a member of the consortium SFC 500-1 able to remove Cr(VI) and simultaneously tolerate high phenol concentrations. In order to elucidate mechanisms utilized by this strain during the bioremediation process, the differential expression pattern of proteins was analyzed when it grew with or without Cr(VI) (10 mg/L) and Cr(VI) + phenol (10 and 300 mg/L), through two complementary proteomic approaches: gel-based (Gel-LC) and gel-free (shotgun) nanoUHPLC-ESI-MS/MS. A total of 400 differentially expressed proteins were identified, out of which 152 proteins were down-regulated under Cr(VI) and 205 up-regulated in the presence of Cr(VI) + phenol, suggesting the extra effort made by the strain to adapt itself and keep growing when phenol was also added. The major metabolic pathways affected include carbohydrate and energetic metabolism, followed by lipid and amino acid metabolism. Particularly interesting were also ABC transporters and the iron-siderophore transporter as well as transcriptional regulators that can bind metals. Stress-associated global response involving the expression of thioredoxins, SOS response, and chaperones appears to be crucial for the survival of this strain under treatment with both contaminants. This research not only provided a deeper understanding of B. toyonensis SFC 500-1E metabolic role in Cr(VI) and phenol bioremediation process but also allowed us to complete an overview of the consortium SFC 500-1 behavior. This may contribute to an improvement in its use as a bioremediation strategy and also provides a baseline for further research.
Topics: Biodegradation, Environmental; Chromium; Phenol; Phenols; Proteomics; Tandem Mass Spectrometry
PubMed: 37209221
DOI: 10.1007/s10534-023-00506-9 -
Journal of the European Academy of... Apr 2022Chemical matricectomy is an established treatment modality of onychocryptosis. In this meta-analysis, we studied the efficacy and safety profile of phenol-based... (Meta-Analysis)
Meta-Analysis Review
Chemical matricectomy is an established treatment modality of onychocryptosis. In this meta-analysis, we studied the efficacy and safety profile of phenol-based matricectomy. We performed an electronic database search of PubMed, EMBASE and grey literature using the search terms '(onychocryptosis OR ingrown toe nail) AND (phenol OR chemical matricectomy)' from inception till 31-12-2020, for controlled clinical trials with phenol in one of the treatment arms and at least 10 participants in each arm. From the initial search of 335, eighteen articles were included in the final analysis. There were a total of 1655 patients, of which 856 received phenol as an intervention modality. We found that nail matrix phenolisation was associated with a 49 fewer number of recurrences per thousand patients compared with other modalities (OR: 0.28-0.57, CI 95%). It also had a reduction in 175 cases of discharge or haemorrhage per thousand patients compared with other modalities (OR: 0.25, 95% CI: 0.14-0.45). However, we found that TCA- and NaOH-based matricectomies fared better compared with phenol in incidence of postoperative discharge and haemorrhage. Patients also experienced less pain (257 fewer number per 1000, OR: 0.52, 95% CI: 0.43-0.63). Nearly, half of the included studies had some concerns about the risk of bias. As of now, phenol matricectomy combines a low recurrence rate with favourable adverse effect profile and is the preferred modality for matricectomy in grade II and III onychocryptosis.
Topics: Humans; Nails; Nails, Ingrown; Phenol; Phenols
PubMed: 34913204
DOI: 10.1111/jdv.17871 -
Journal of Bacteriology Apr 2022Bacillus subtilis employs 10 chemoreceptors to move in response to chemicals in its environment. While the sensing mechanisms have been determined for many attractants,...
Bacillus subtilis employs 10 chemoreceptors to move in response to chemicals in its environment. While the sensing mechanisms have been determined for many attractants, little is known about the sensing mechanisms for repellents. In this work, we investigated phenol chemotaxis in B. subtilis. Phenol is an attractant at low, micromolar concentrations and a repellent at high, millimolar concentrations. McpA was found to be the principal chemoreceptor governing the repellent response to phenol and other related aromatic compounds. In addition, the chemoreceptors McpC and HemAT were found to govern the attractant response to phenol and related compounds. Using chemoreceptor chimeras, McpA was found to sense phenol using its signaling domain rather than its sensing domain. These observations were substantiated , where direct binding of phenol to the signaling domain of McpA was observed using saturation transfer difference nuclear magnetic resonance. These results further advance our understanding of B. subtilis chemotaxis and further demonstrate that the signaling domain of B. subtilis chemoreceptors can directly sense chemoeffectors. Bacterial chemotaxis is commonly thought to employ a sensing mechanism involving the extracellular sensing domain of chemoreceptors. Some ligands, however, appear to be sensed by the signaling domain. Phenolic compounds, commonly found in soil and root exudates, provide environmental cues for soil microbes like Bacillus subtilis. We show that phenol is sensed as both an attractant and a repellent. While the mechanism for sensing phenol as an attractant is still unknown, we found that phenol is sensed as a repellent by the signaling domain of the chemoreceptor McpA. This study furthers our understanding of the unconventional sensing mechanisms employed by the B. subtilis chemotaxis pathway.
Topics: 2-Methyl-4-chlorophenoxyacetic Acid; Bacillus subtilis; Bacterial Proteins; Chemotaxis; Phenol; Phenols; Soil
PubMed: 35007157
DOI: 10.1128/JB.00441-21 -
Environmental Science and Pollution... May 2021V-doped TiO materials (0.01, 0.05, 0.10, and 1.00 nominal atomic %) were synthesized by the sol-gel method and characterized by X-ray diffraction, Raman spectroscopy,...
V-doped TiO materials (0.01, 0.05, 0.10, and 1.00 nominal atomic %) were synthesized by the sol-gel method and characterized by X-ray diffraction, Raman spectroscopy, UV-visible diffuse reflectance spectroscopy, N adsorption-desorption isotherms, X-ray photoelectron spectroscopy, and H-temperature programmed reduction. Two vanadium precursors (vanadyl acetylacetonate and ammonium metavanadate) and three calcination temperatures (400, 500, and 600 °C, with and without air circulation) were assayed. The efficiency of the materials as photocatalysts was studied by the degradation of phenol with UV and visible lamps. The photocatalyst prepared from vanadium acetylacetonate, with a vanadium content of 0.01 nominal atomic %, calcination at 400 °C without air circulation (0.01VTi-400), showed the best performance, reaching 100% and 30% degradation of phenol (50 μM) by irradiation with UV lamps (3 h) and visible lamps (5 h), respectively. To evaluate the efficiency of this catalyst in the degradation of other structurally related compounds, two substituted phenols were selected: 4-chlorophenol and 4-nitrophenol. The 0.01VTi-400 photocatalyst showed to be applicable to the degradation of phenolic compounds when the substituent was an activating group or a weakly deactivating group (for electrophilic reactions). Additionally, the selectivity of 0.01VTi-400 for phenol degradation in the presence of Aldrich humic acid was tested: phenol degradation reached 68% (3 h, UV lamps). The performance of 0.01VTi-400 indicated that it is a promising material for further applications.
Topics: Catalysis; Environmental Pollutants; Phenol; Titanium; X-Ray Diffraction
PubMed: 33471310
DOI: 10.1007/s11356-021-12339-5 -
MicrobiologyOpen Oct 2022Five yeast fungi strains (i.e., two Cryptococcus albidus, one Candida guillermondii, and two Candida tropicalis) were isolated from sugarcane and tested for their use of...
Five yeast fungi strains (i.e., two Cryptococcus albidus, one Candida guillermondii, and two Candida tropicalis) were isolated from sugarcane and tested for their use of lignin as sole carbon source and their potential to grow in the presence of phenol and phenol derivatives (i.e., pentachlorophenol and p-nitrophenol). The full set of isolated yeasts showed ligninolytic activity, achieving at least 36% lignin degradation after 25 days. The C. albidus JS-B1 strain had the highest ligninolytic activity, achieving 27% lignin degradation within 4 days. This increased activity was associated with the production of ligninolytic laccase enzymes. All the tested yeast fungi strains showed growth in the presence of high concentrations of phenolic compounds (i.e., 900 mg/L phenol, 200 mg/L p-nitrophenol, 50 mg/L pentachlorophenol) and showed significant potential for lignin and lignin by-product degradation. Each of these five strains has the potential to be used in biological treatment processes for contaminated effluents from paper pulping and bleaching or phenol and phenol-derivative biodegradation processes for other industrial wastewater effluents.
Topics: Lignin; Pentachlorophenol; Nitrophenols; Yeasts; Phenol; Biodegradation, Environmental; Phenols
PubMed: 36314751
DOI: 10.1002/mbo3.1326 -
Biointerphases Jul 2023Horseradish peroxidase (HRP) is a hemoglobin composed of a single peptide chain that catalyzes the oxidation of various substrates such as phenol and aniline in the...
Horseradish peroxidase (HRP) is a hemoglobin composed of a single peptide chain that catalyzes the oxidation of various substrates such as phenol and aniline in the presence of hydrogen peroxide via its iron-porphyrin catalytic center. This enzyme is widely used in industrial phenol removal, food additives, biomedicine, and clinical test reagents due to its rapid reaction rate and obvious reaction outcomes. However, the large-scale use of HRP in industrial applications still faces numerous challenges, including activity, stability, and sustainability. This study demonstrates that when peroxidase is immobilized in zwitterionic polymer hydrogels, polycarboxybetaine (PCB) and polysulfobetaine (PSB), the properties of the enzyme are improved. PCB and PSB-embedded HRP exhibit a 6.11 and 1.53 times increase in Kcat/Km value, respectively, compared to the free enzyme. The immobilized enzyme also experiences increased activity over a range of temperatures and better tolerance to extreme pH and organic solvents, including formaldehyde. In addition, immobilized HRP exhibits excellent performance in storage and reproducibility. Remarkably, PCB-HRP still retains 80% of the initial activity after a 6-week storage period and can still attain the initial catalytic level of the free enzyme after six repeated cycles. It also removes 90% of phenol within 12 min, surpassing the current pharmacy on the market. These experimental results indicated that we have successfully designed a set of stable and efficient support substrates for horseradish peroxidase, which enhances its suitability for deployment in industrial applications.
Topics: Polymers; Enzyme Stability; Horseradish Peroxidase; Reproducibility of Results; Phenols; Phenol; Enzymes, Immobilized
PubMed: 37410499
DOI: 10.1116/6.0002657 -
Environmental Research Jul 2023Review papers help researchers understand the direction and flow of research and help beginners quickly acquire relevant knowledge. Therefore, the review paper should... (Review)
Review
Comment on the review paper "agricultural waste materials for adsorptive removal of phenol, chromium(VI) and cadmium(II) from wastewaters: A review" by Othmani et al. (2022).
Review papers help researchers understand the direction and flow of research and help beginners quickly acquire relevant knowledge. Therefore, the review paper should describe only accurate contents and should be written with only core and important matters. Recently, Othmani et al. (2022) reviewed more than 200 papers related to the adsorptive removal of three harmful pollutants: toxic organics (phenols), anionic heavy metal (Cr(VI)) and cationic heavy metal (Cd(II)) by agricultural waste materials. However, Cr(VI)-related section of this review is missing something important that should be provided to the readers of this journal. In fact, the removal mechanism of Cr(VI) by nonliving biomass including agricultural waste materials under acidic conditions is not a simple anion adsorption but a complex mechanism involving redox reaction called "adsorption-coupled reduction". Therefore, when interpreting the factors affecting Cr(VI) removal, it should be interpreted in terms of the redox reaction concept, not anion adsorption.
Topics: Cadmium; Wastewater; Phenol; Adsorption; Chromium; Waste Products; Phenols
PubMed: 37080280
DOI: 10.1016/j.envres.2023.115859 -
Food & Function Oct 2023: Estimating (poly)phenol intake is challenging due to inadequate dietary assessment tools and limited food content data. Currently, diet scores to characterise...
: Estimating (poly)phenol intake is challenging due to inadequate dietary assessment tools and limited food content data. Currently, diet scores to characterise (poly)phenol-rich diets are lacking. This study aimed to develop a novel (poly)phenol-rich diet score (PPS) and explore its relationship with circulating (poly)phenol metabolites. : A total of 543 healthy free-living participants aged 18-80 years completed a food frequency questionnaire (FFQ) (EPIC-Norfolk) and provided 24 h urine samples. The PPS was developed based on the relative intake (quintiles) of 20 selected (poly)phenol-rich food items abundant in the UK diet, including tea, coffee, red wine, whole grains, chocolate and cocoa products, berries, apples and juice, pears, grapes, plums, citrus fruits and juice, potatoes and carrots, onions, peppers, garlic, green vegetables, pulses, soy and soy products, nuts, and olive oil. Foods included in the PPS were chosen based on their (poly)phenol content, main sources of (poly)phenols, and consumption frequencies in the UK population. Associations between the PPS and urinary phenolic metabolites were investigated using linear models adjusting energy intake and multiple testing (FDR adjusted < 0.05). : The total PPS ranged from 25 to 88, with a mean score of 54. A total of 51 individual urinary metabolites were significantly associated with the PPS, including 39 phenolic acids, 5 flavonoids, 3 lignans, 2 resveratrol and 2 other (poly)phenol metabolites. The total (poly)phenol intake derived from FFQs also showed a positive association with PPS (stdBeta 0.32, 95% CI (0.24, 0.40), < 0.01). Significant positive associations were observed in 24 of 27 classes and subclasses of estimated (poly)phenol intake and PPS, with stdBeta values ranging from 0.12 (0.04, 0.20) for theaflavins/thearubigins to 0.43 (0.34, 0.51) for flavonols ( < 0.01). : High adherence to the PPS diet is associated with (poly)phenol intake and urinary biomarkers, indicating the utility of the PPS to characterise diets rich in (poly)phenols at a population level.
Topics: Humans; Phenol; Polyphenols; Phenols; Diet; Fruit; Antioxidants
PubMed: 37840467
DOI: 10.1039/d3fo01982a -
Natural Product Research Dec 2022In this study, NMR and GC-MS based comparative metabolomic profiling of the roots of three different species namely, , and were investigated followed by multivariate...
In this study, NMR and GC-MS based comparative metabolomic profiling of the roots of three different species namely, , and were investigated followed by multivariate statistical analyses and their antioxidant activity. Also, two alkylated phenols i.e., 2,6-di--butyl phenol and 2,4-di--butyl phenol not reported earlier from this taxon were isolated from . This metabolic study resulted in the identification of 25 and quantification of 18 metabolites. Principal component analysis showed the clear distinction among the three species. The antioxidant activity in the extracts was tested by free radical scavenging method. The three species revealed interesting antioxidant potential, in particular, , which was rich in naphthoquinones, coumarins, alkylated phenols, sterols, triterpenes, fatty acids showed lowest IC value. The results highlighted the role of in the management of oxidative stress especially when they are utilized in the formation of fermented food products.
Topics: Antioxidants; Phenols; Plumbaginaceae; Gas Chromatography-Mass Spectrometry; Phenol; Metabolomics; Plant Extracts
PubMed: 35337230
DOI: 10.1080/14786419.2022.2055014 -
Chemosphere Apr 2023Hydrothermal liquefaction (HTL) is an attractive technology for the conversion of wet waste into biofuel and co-HTL has been touted to increase the quality of products....
Hydrothermal liquefaction (HTL) is an attractive technology for the conversion of wet waste into biofuel and co-HTL has been touted to increase the quality of products. However, the recovery of energy from wastewater byproduct called aqueous co-product (ACP) is limited due to the presence of toxic inhibitory substances. Adsorption has been countenanced to remove these toxic compounds but there has not been a distinct comprehensive adsorption isotherm study to explain the interaction between the adsorbate molecules and the adsorbent sites. This study investigated the sorption mechanism of oxidizable reducing pollutants measured as chemical oxygen demand (COD); heavy metals (boron and copper); and phenols from ACP samples obtained from co-HTL of brewery trub (BT), and primary sludge (PS) onto granular and powdered activated carbon (GAC and PAC). Conventional isotherm models such as Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich were used for data analysis. Results indicated that the adsorptive capacity (qe) of PAC was greater than GAC in COD adsorption (BT-1947 > 234; BTPS-617 > 245; PS-289 > 207), boron adsorption (BTPS-70 > 7; PS-53 > 49), copper adsorption (BT-5 > 1; BTPS-3 > 2; PS-1.3 > 1.1) and phenol adsorption (BT-1340 > 356; BTPS-1587 > 253; PS-460 > 245) in mg/g, μg/g, μg/g, and μg/g respectively. Comparing the adsorption of pollutants onto PAC and GAC, this study observed that PAC followed the Temkin, and Dubinin-Radushkevich models in the adsorption of the four pollutants while GAC followed the Freundlich and Langmuir models in the adsorption of phenol and copper, and Temkin, and Dubinin-Radushkevich in the adsorption of COD and boron. This study proved that combining feedstock in HTL (co-HTL) does not only change the quality of the ACP but also changes the dynamics of the adsorption isotherms. The Free Energy Change (ΔG) result showed a spontaneous reaction in the adsorption of copper and phenol. This study presents an adsorption equilibrium information for the interpretation of adsorption isotherms for the overall improvement of adsorption mechanism pathways and the effective design of adsorption systems for the treatment of ACP.
Topics: Phenol; Environmental Pollutants; Adsorption; Thermodynamics; Water Pollutants, Chemical; Copper; Boron; Phenols; Sewage; Kinetics; Hydrogen-Ion Concentration
PubMed: 36804248
DOI: 10.1016/j.chemosphere.2023.138165