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Environmental Science & Technology Feb 2022Although chlorinated organophosphate esters (Cl-OPEs) have been reported to be ubiquitously distributed in various anoxic environments, little information is available...
Although chlorinated organophosphate esters (Cl-OPEs) have been reported to be ubiquitously distributed in various anoxic environments, little information is available on their fate under anoxic conditions. In this study, we report two -containing enrichment cultures that transformed 3.88 ± 0.22 μmol tris(2-chloroethyl) phosphate (TCEP) and 2.61 ± 0.02 μmol tris(1-chloro-2-propyl) phosphate (TCPP) within 10 days. Based on the identification of the transformed products and deuteration experiments, we inferred that TCEP may be transformed to generate bis(2-chloroethyl) phosphate and ethene via one-electron transfer (radical mechanism), followed by C-O bond cleavage. Ethene was subsequently reduced to ethane. Similarly, TCPP was transformed to form bis(1-chloro-2-propyl) phosphate and propene. 16S rRNA gene amplicon sequencing and quantitative polymerase chain reaction analysis revealed that was the predominant contributor to the transformation of TCEP and TCPP. Two draft genomes of assembled from the metagenomes of the TCEP- and TCPP-transforming enrichment cultures contained 14 and 15 putative reductive dehalogenase () genes, respectively. Most of these genes were actively transcribed, suggesting that they might contribute to the transformation of TCEP and TCPP. Taken together, this study provides insights into the role of during the transformation of representative Cl-OPEs.
Topics: Dehalococcoides; Esters; Flame Retardants; Organophosphates; Phosphates; RNA, Ribosomal, 16S
PubMed: 35015551
DOI: 10.1021/acs.est.1c06686 -
The Science of the Total Environment Jun 2021Organophosphate esters (OPEs) are common additives in a wide range of commercial and industrial products. Elevated and prolonged exposure to OPEs may induce several... (Meta-Analysis)
Meta-Analysis Review
Organophosphate esters (OPEs) are common additives in a wide range of commercial and industrial products. Elevated and prolonged exposure to OPEs may induce several adverse effects. This is concerning as they are ubiquitous in air, indoor dust, drinking water, and other environmental matrices. However, information on the presence of OPEs in foodstuffs and consequent health risks remains scant. This review critically evaluates available information on levels and sources of OPEs in food, discusses the relative significance of diet as a pathway of human exposure, identifies knowledge gaps, and suggests directions for future research. For toddlers, dermal uptake from dust ingestion appears the predominant pathway of exposure to chlorinated OPEs, as well as ethylhexyl diphenyl phosphate (EHDPP) and triphenyl phosphate (TPHP). In contrast, diet appears the main pathway of exposure to all eight OPEs considered for adults, and for tri n-butyl phosphate (TnBP), tris 2-ethylhexyl phosphate (TEHP), and tris (2-butoxyethyl) phosphate (TBOEP) for toddlers. While summed exposures via all pathways are within reference dose (RfD) values, they do not include high-end exposure estimates, and for highly-exposed individuals, the margin between exposure and RfD values is smaller. Moreover, our exposure estimates are based on a meta-analysis of multiple exposure assessments conducted over a range of points in space and time. There is an urgent need for assessments of human exposure to OPEs that examine all relevant pathways in a spatially and temporally-consistent fashion. Given food is an important exposure pathway to OPEs, regular monitoring of their presence as well as their metabolites (that may have toxicological significance) in foodstuffs is recommended. While dermal uptake from indoor dust appears an important human exposure pathway, no evaluations exist of exposure via dermal uptake from OPE-containing products such as foam-filled furniture. This review also highlights very few data exist on OPEs in drinking water.
Topics: Adult; China; Dust; Eating; Environmental Exposure; Environmental Monitoring; Esters; Flame Retardants; Humans; Organophosphates
PubMed: 33540161
DOI: 10.1016/j.scitotenv.2020.144752 -
International Journal of Environmental... Feb 2023Paraoxonase-1 (PON1) is a calcium-dependent, HDL-bound serum hydrolase active toward a wide variety of substrates. PON1 displays three types of activities, among which... (Review)
Review
Paraoxonase-1 (PON1) is a calcium-dependent, HDL-bound serum hydrolase active toward a wide variety of substrates. PON1 displays three types of activities, among which lactonase, paraoxonase, arylesterase and phosphotriesterase can be distinguished. Not only is this enzyme a major organophosphate compound detoxifier, but it is also an important constituent of the cellular antioxidant system and has anti-inflammatory and antiatherogenic functions. The concentration and activity of PON1 is highly variable among individuals, and these differences can be both of genetic origin and be a subject of epigenetic regulation. Owing to the fact that, in recent decades, the exposure of humans to an increasing number of different xenobiotics has been continuously rising, the issues concerning the role and activity of PON1 shall be reconsidered with particular attention to growing pharmaceuticals intake, dietary habits and environmental awareness. In the following manuscript, the current state of knowledge concerning the influence of certain modifiable and unmodifiable factors, including smoking, alcohol intake, gender, age and genotype variation on PON1 activity, along with pathways through which these could interfere with the enzyme's protective functions, is presented and discussed. Since exposure to certain xenobiotics plays a key role in PON1 activity, the influence of organophosphates, heavy metals and several pharmaceutical agents is also specified.
Topics: Humans; Aryldialkylphosphatase; Epigenesis, Genetic; Antioxidants; Smoking; Organophosphates; Life Style
PubMed: 36833509
DOI: 10.3390/ijerph20042813 -
The Science of the Total Environment Jun 2023Electronic waste (e-waste) dismantling facilities are a well-known source of emerging contaminants including organophosphate esters (OPEs). However, little information...
Electronic waste (e-waste) dismantling facilities are a well-known source of emerging contaminants including organophosphate esters (OPEs). However, little information is available regarding the release characteristics and co-contaminations of tri- and di-esters. This study, therefore, investigated a broad range of tri- and di-OPEs in dust and hand wipe samples collected from an e-waste dismantling plant and homes as comparison. The median ∑tri-OPE and ∑di-OPE levels in dust and hand wipe samples were approximately 7- and 2-fold higher than those in the comparison group, respectively (p < 0.01). Triphenyl phosphate (median: 11,700 ng/g and 4640 ng/m) and bis(2-ethylhexyl) phosphate (median: 5130 ng/g and 940 ng/m) were the dominant components of tri- and di-OPEs, respectively. The combination of Spearman rank correlations and the determinations of molar concentration ratios of di-OPEs to tri- OPEs revealed that apart from the degradation of tri-OPEs, di-OPEs could originate from direct commercial application, or as impurities in tri-OPE formulas. Significant positive correlations (p < 0.05) were found for most tri- and di-OPE levels between the dust and hand wipes from dismantling workers, whereas this was not observed in those from the ordinary microenvironment. Our results provide robust evidence that e-waste dismantling activities contribute to OPEs contamination in the surroundings and further human exposure pathways and toxicokinetics are needed to be elucidated.
Topics: Humans; Electronic Waste; Dust; Esters; Flame Retardants; Organophosphates; China; Environmental Monitoring
PubMed: 37003336
DOI: 10.1016/j.scitotenv.2023.163176 -
Bulletin of Environmental Contamination... May 2022Since the phase-out of traditional halogenated flame retardants (HFRs), interests of research are gradually being shifted to organophosphate flame retardants (OPFRs),... (Review)
Review
Since the phase-out of traditional halogenated flame retardants (HFRs), interests of research are gradually being shifted to organophosphate flame retardants (OPFRs), and this can be reflected by the increasing number of publications on OPFRs year by year. Here, an extensive survey is conducted in an attempt to generate a list of OPFRs that are being produced in factories, and to investigate the annual production volume (APV). This survey suggests that at least n = 56 OPFR monomers and n = 62 OPFR mixtures are being currently produced in 367 factories around the world, and 201 out of them are in Mainland China. APV of OPFRs was estimated as 598,422 metric tons, and this number could be underestimated due to the limitation of available information. We also notice that current researches are confined to a limited number of OPFRs, especially for OP esters (OPEs), and other OPFRs with different structures from OPEs has been rarely studied. Based on all the collected datasets, we provide five recommendations for how to proceed with future research to more comprehensively understand the currently-produced OPFRs in the environment.
Topics: China; Flame Retardants; Organophosphates
PubMed: 35080673
DOI: 10.1007/s00128-021-03454-7 -
Environment International Jan 2021Organophosphate esters (OPEs) are widely used as flame retardants and plasticizers in consumer and industrial products. Human exposure to OPEs raises concerns due to...
Organophosphate esters (OPEs) are widely used as flame retardants and plasticizers in consumer and industrial products. Human exposure to OPEs raises concerns due to their endocrine disruptive potentials. Till now, the effects of OPEs on thyroid hormones (THs) and the mediating role of oxidative stress in pregnant women have not been studied. In this study, prenatal urinary concentrations of OPE metabolites (mOPEs), levels of free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), and oxidative stress levels of 8-hydroxy-2-deoxy guanosine (8-OHdG) and malondialdehyde (MDA) were measured in pregnant women (n = 360) from a coastal urbanized region and moderate socioeconomic status. Neonatal TSH in heel blood was also measured in newborns (n = 309). Dibutyl phosphate (DBP) and diphenyl phosphate (DPHP) were extensively detected with a median creatinine-adjusted level of 0.19 μg/g and 0.66 μg/g, respectively, and the median of ∑mOPEs was 1.82 μg/g. DBP and DPHP were included in the analysis. The concentrations of DBP and DPHP were positively associated with either maternal or neonatal TSH levels, while not for maternal FT3 and FT4 levels. Positive associations for maternal and neonatal TSH were particularly observed in girls as stratified by newborn sex suggesting a sex-selective difference. Furthermore, 8-OHdG, the biomarker of DNA damage, was found to be a major mediator (>60%) for the association between neonatal TSH and DPHP, suggesting that DNA damage is involved in fetal thyroid function disruption. On the other hand, MDA showed a partially suppressing effect (<40%) for the associations between mOPEs and neonatal TSH, which needs further clarification. For maternal TSH, both 8-OHdG and MDA showed moderate mediating effects while the direct effects of mOPEs on maternal TSH also contributed. These results suggest thyroid disrupting effects of OPE exposure on mothers and fetuses during pregnancy and the potential influence mediated by the oxidative stresses of DNA damage and lipid peroxidation.
Topics: Esters; Female; Flame Retardants; Humans; Infant, Newborn; Organophosphates; Oxidative Stress; Plasticizers; Pregnancy; Thyroid Gland
PubMed: 33113466
DOI: 10.1016/j.envint.2020.106215 -
Environmental Geochemistry and Health Nov 2020Since the addition of polybrominated diphenyls and organochlorine pesticides (OCPs) to the world banned list, toxic organophosphate contaminants (OPCs) such as... (Review)
Review
Since the addition of polybrominated diphenyls and organochlorine pesticides (OCPs) to the world banned list, toxic organophosphate contaminants (OPCs) such as organophosphate flame retardants and organophosphate pesticides have been, respectively, used as substitutes. These chemicals are reported to be more toxic than their halogenated counterparts. It is rare to find a study that focuses on visualising the publication trends of these chemical classes. In this study, we employed a bibliometric model to systematically map research activities between 1990 and 2018 using OPC articles retrieved from the WoS and Scopus databases. A total of 1090 articles were retrieved from the hybrid databases with an article/author and author/article ratio of 0.33 and 3.02, respectively. Articles on OPC studies were positively correlated with the number of years (r = 0.96; y = 0.23x - 3.82x + 27.90) suggesting an increase in the number of articles on this subject in future. The USA ranked first in terms of articles (n = 245) and citations (n = 12,922) followed by China and India (203 and 89 articles, respectively). Articles from China and the USA had strong collaboration with other countries. Research priorities and top author keywords included pesticides (n = 112), organophosphate (n = 83) and acetylcholinesterase (n = 60) and were also well represented in keywords-plus. Developed countries had higher outputs compared to developing countries. It was observed that from our thematic literature classifications, human toxicity, ecotoxicological impacts, and environmental monitoring of OPCs were of greater importance to scholars, thus indicating the direction of future research. Futuristic studies need to foster partnership with policymakers, journalists, consultants, farmers, artisans and community workers on OPC research. This will not only enhance scientific communication and community engagement but will also increase the awareness of these pollutants to the general public.
Topics: Animals; Bibliometrics; China; Databases, Bibliographic; Environmental Monitoring; Environmental Pollutants; Flame Retardants; Humans; India; Organophosphates; Pesticides; Serial Publications
PubMed: 32435924
DOI: 10.1007/s10653-020-00594-3 -
Rural and Remote Health Jan 2020Organophosphates are a class of insecticides used globally by the agricultural industry for insect control. Acute consequences of organophosphate exposures are well... (Review)
Review
INTRODUCTION
Organophosphates are a class of insecticides used globally by the agricultural industry for insect control. Acute consequences of organophosphate exposures are well known, while there has been limited research on their long-term effects. The objective of this review was to discuss the health effects of chronic organophosphate exposure in farmers.
METHODS
Medline, Scopus and Web of Science were searched to find the relevant articles. Articles published only in English and until December 2018 were reviewed. The selected articles were then categorised as neurological (neurobehaviour, neurodevelopmental, neurological signs and symptoms) or non-neurological subheadings.
RESULTS
A total of 53 articles for neurological effects and 17 articles for non-neurological effects were identified. Chronic organophosphates exposure was associated with deficits in the neurobehaviour subsets of attention and short-term memory, increased incidence of neurodegenerative diseases and effects on peripheral nerves and neurodevelopment. However, research to support non-neurological effects such as respiratory symptoms, increased cancer risk, endocrine disruption, cardiac issues, chronic fatigue and infertility was limited.
CONCLUSION
Chronic organophosphate exposure was found to affect four of the five areas of described neurological effects in the literature. A large proportion of the research in this area was not methodologically strong, therefore few recommendations can be conclusively made. Future research is warranted to investigate the non-neurological effects of chronic exposure to ensure the occupational risks of low-level chronic exposure are clearly communicated to farmers and farm workers.
Topics: Agricultural Workers' Diseases; Farmers; Humans; Insecticides; Occupational Exposure; Organophosphates
PubMed: 31902214
DOI: 10.22605/RRH4508 -
Journal of Environmental Sciences... Mar 2024Lipid metabolism play an essential role in occurrence and development of asthma, and it can be disturbed by phthalate esters (PAEs) and organophosphate flame retardants...
Lipid metabolism play an essential role in occurrence and development of asthma, and it can be disturbed by phthalate esters (PAEs) and organophosphate flame retardants (OPFRs). As a chronic inflammatory respiratory disease, the occurrence risk of childhood asthma is increased by PAEs and OPFRs exposure, but it remains not entirely clear how PAEs and OPFRs contribute the onset and progress of the disease. We have profiled the serum levels of PAEs and OPFRs congeners by liquid chromatography coupled with mass spectrometry, and its relationships with the dysregulation of lipid metabolism in asthmatic, bronchitic (acute inflammation) and healthy (non-inflammation) children. Eight PAEs and nine OPFRs congeners were found in the serum of children (1 - 5 years old) from Shenzhen, and their total median levels were 615.16 ng/mL and 17.06 ng/mL, respectively. Moreover, the serum levels of mono-methyl phthalate (MMP), tri-propyl phosphate (TPP) and tri-n-butyl phosphate (TNBP) were significant higher in asthmatic children than in healthy and bronchitic children as control. Thirty-one characteristic lipids and fatty acids of asthma were screened by machine-learning random forest model based on serum lipidome data, and the alterations of inflammatory characteristic lipids and fatty acids including palmitic acids, 12,13-DiHODE, 14,21-DiHDHA, prostaglandin D2 and LysoPA(18:2) showed significant correlated with high serum levels of MMP, TPP and TNBP. These results imply PAEs and OPFRs promote the occurrence of childhood asthma via disrupting inflammatory lipid and fatty acid metabolism, and provide a novel sight for better understanding the effects of plastic additives on childhood asthma.
Topics: Child; Humans; Infant; Child, Preschool; Fatty Acids; Organophosphates; Asthma; Flame Retardants
PubMed: 37980005
DOI: 10.1016/j.jes.2023.02.005 -
Environmental Research Jun 2023Organophosphate esters (OPEs) are used as flame retardants and plasticizers in various consumer products. Limited prior research suggests sex-specific effects of...
Sex-specific effects of prenatal organophosphate ester (OPE) metabolite mixtures and adverse infant birth outcomes in the maternal and developmental risks from environmental and social stressors (MADRES) pregnancy cohort.
BACKGROUND
Organophosphate esters (OPEs) are used as flame retardants and plasticizers in various consumer products. Limited prior research suggests sex-specific effects of prenatal OPE exposures on fetal development. We evaluated overall and sex-specific associations between prenatal OPE exposures and gestational age (GA) at birth and birthweight for gestational age (BW for GA) z-scores among the predominately low-income, Hispanic MADRES cohort.
METHODS
Nine OPE metabolite concentrations were measured in 421 maternal urine samples collected during a third trimester visit (GA = 31.5 ± 2.0 weeks). We examined associations between single urinary OPE metabolites and GA at birth and BW for GA z-scores using linear regression models and Generalized Additive Models (GAMs) and effects from OPE mixtures using Bayesian Kernel Machine Regression (BKMR). We also assessed sex-specific differences in single metabolite analyses by evaluating statistical interactions and stratifying by sex.
RESULTS
We did not find significant associations between individual OPE metabolites and birth outcomes in the full infant sample; however, we found that higher bis(1,3-dichloro-2-propyl) phosphate (BDCIPP) was associated with earlier GA at birth among male infants (p = 0.04), and a nonlinear, inverted U-shape association between the sum of dibutyl phosphate and di-isobutyl phosphate (DNBP + DIBP) and GA at birth among female infants (p = 0.03). In mixtures analysis, higher OPE metabolite mixture exposures was associated with lower GA at birth, which was primarily driven by female infants. No associations were observed between OPE mixtures and BW for GA z-scores.
CONCLUSION
Higher BDCIPP and DNBP + DIBP concentrations were associated with earlier GA at birth among male and female infants, respectively. Higher exposure to OPE mixtures was associated with earlier GA at birth, particularly among female infants. However, we saw no associations between prenatal OPEs and BW for GA. Our results suggest sex-specific impacts of prenatal OPE exposures on GA at birth.
Topics: Pregnancy; Infant, Newborn; Humans; Male; Infant; Female; Bayes Theorem; Organophosphates; Phosphates; Flame Retardants; Esters
PubMed: 36934865
DOI: 10.1016/j.envres.2023.115703