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Nature Communications Jan 2022Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose...
Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric optical design, we present a low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to follow natural curvatures in biological structures. Crucially, our core design is readily implemented (and reversed) within a matter of hours, making it highly suitable as a base platform for further development. We demonstrate the application of our system for imaging neuronal activity in a variety of examples in zebrafish, mice and fruit flies.
Topics: Animals; Brain; Drosophila; Imaging, Three-Dimensional; Larva; Lenses; Light; Male; Mice; Microscopy; Neurons; Photons; Zebrafish
PubMed: 35087041
DOI: 10.1038/s41467-022-28192-0 -
Journal of Biophotonics Nov 2020We experimentally investigate the two-photon absorption cross sections and spectra of eosin and hematoxylin for applications in nonlinear microscopy. The experiments are...
We experimentally investigate the two-photon absorption cross sections and spectra of eosin and hematoxylin for applications in nonlinear microscopy. The experiments are carried out on pure samples of the two dyes in DI-water solvent with different concentrations, in the typical range employed in standard staining procedures. Nonlinear fluorescence is excited by a line-shaped beam emitted by a Ti:Sapphire mode-locked laser in the wavelength range from 740 to 880 nm and is detected through a microscope setup. The two-photon absorption spectral response is systematically analyzed and discussed. Finally, the staining is applied on biological tissue samples, which are imaged by two-photon microscopy. Our results show that the employed dyes are fully suitable for applications in nonlinear imaging.
Topics: Eosine Yellowish-(YS); Hematoxylin; Microscopy; Photons; Staining and Labeling
PubMed: 32713128
DOI: 10.1002/jbio.202000141 -
Journal of the Optical Society of... Dec 2022The groundbreaking research and ideas introduced by Emil Wolf continue to inspire researchers and motivate ongoing research in the wave properties of light. This special...
The groundbreaking research and ideas introduced by Emil Wolf continue to inspire researchers and motivate ongoing research in the wave properties of light. This special issue commemorates the legacy of Emil Wolf with research in physical optics, with specific focus on those areas where Wolf was active, such as optical coherence theory, inverse problems, singular optics, imaging, and polarization, and the intersection of these fields of study. Here we discuss the life of Emil Wolf and his influence on optical science and the optics community.
Topics: Animals; Wolves; Optics and Photonics
PubMed: 36520761
DOI: 10.1364/JOSAA.481695 -
Optics Letters Jul 2023Variational quantum algorithms (VQAs) combining the advantages of parameterized quantum circuits and classical optimizers, promise practical quantum applications in the...
Variational quantum algorithms (VQAs) combining the advantages of parameterized quantum circuits and classical optimizers, promise practical quantum applications in the noisy intermediate-scale quantum era. The performance of VQAs heavily depends on the optimization method. Compared with gradient-free and ordinary gradient descent methods, the quantum natural gradient (QNG), which mirrors the geometric structure of the parameter space, can achieve faster convergence and avoid local minima more easily, thereby reducing the cost of circuit executions. We utilized a fully programmable photonic chip to experimentally estimate the QNG in photonics for the first time, to the best of our knowledge. We obtained the dissociation curve of the He-H cation and achieved chemical accuracy, verifying the outperformance of QNG optimization on a photonic device. Our work opens up a vista of utilizing QNG in photonics to implement practical near-term quantum applications.
Topics: Optics and Photonics; Algorithms; Photons
PubMed: 37450740
DOI: 10.1364/OL.494560 -
Nanotechnology Jul 2023Selenium (Se) is located in the fourth period of the periodic table in group VIA (element 34). In this experiment, three different solvents (isopropyl alcohol,...
Selenium (Se) is located in the fourth period of the periodic table in group VIA (element 34). In this experiment, three different solvents (isopropyl alcohol, N-methyl-2-pyrrolidone, and ethanol) were used to prepare the two-dimensional Se nanosheets, which were manufactured by the liquid phase exfoliation method with a thickness of 3.35-4.64 nm and a transverse scale of several hundred nanometers. The nonlinear absorption properties at 355, 532, and 1064 nm were studied using the open aperture-scan technique. Final results showed that Se nanosheets exhibited optical limiting (OL) effect in all three wavebands and three solvents, and had large two-photon absorption coefficients, especially in ultraviolet (UV) waveband. Which proved that Se nanosheets had great potential application as excellent OL materials in UV waveband. Our research broadens the path for the semiconductor field of Se, inspires the application of Se in nonlinear optics field.
Topics: Selenium; Solvents; Optics and Photonics; Photons
PubMed: 37315540
DOI: 10.1088/1361-6528/acde59 -
Cells Feb 2024This Editorial is the preface for the topical collection of "Computational Imaging for Biophotonics and Biomedicine", which collates the 12 contributions listed in Table...
This Editorial is the preface for the topical collection of "Computational Imaging for Biophotonics and Biomedicine", which collates the 12 contributions listed in Table 1 [...].
Topics: Optics and Photonics
PubMed: 38474397
DOI: 10.3390/cells13050433 -
Environmental Science and Pollution... Jul 2023We are honored to present this special issue of the Environmental Science and Pollution Research which comprises the selected papers presented at the international...
We are honored to present this special issue of the Environmental Science and Pollution Research which comprises the selected papers presented at the international conference on Advanced Materials for Photonics, Sensing and Energy Conversion Energy Applications, held in Marrakech, Morocco.
Topics: Optics and Photonics; Morocco; Environmental Science
PubMed: 37335509
DOI: 10.1007/s11356-023-27991-2 -
Technology in Cancer Research &... 2022The aim of this study is to measure the output factor (OF) and profile of surface dose in regular and small radiation therapy fields using Cherenkov imaging (CI). A...
The aim of this study is to measure the output factor (OF) and profile of surface dose in regular and small radiation therapy fields using Cherenkov imaging (CI). A medical linear accelerator (linac) was employed to generate radiation fields, including regular open photon field (ROPF), regular wedge photon field (RWPF), regular electron field (REF) and small photon field (SPF). The photon beams consisted of two filter modes including flattening filter (FF) and flattening filter free (FFF). All fields were delivered to a solid water phantom. Cherenkov light was captured using a charge-coupled device system during phantom irradiation. The OF and profile of surface dose measured by CI were compared with those determined by film measurement, ionization chamber measurement and treatment planning system calculation in order to examine the feasibility of measuring surface dose OF and profile using CI. The discrepancy between surface dose OF measured by CI and that determined by other methods is less than 6% in ROPFs with size less than 10 × 10 cm, REFs with size less than 10 × 10 cm, and SPFs except for 1 × 1 cm field. In the flat profile region, the discrepancy between surface dose profile measured by CI and that determined by other methods is less than 4% in REFs and less than 3% in ROPFs, RWPFs, and SPFs except for 1 × 1 cm2 field. The discrepancy of the surface dose profile is in compliance with the recommendation by IAEA TRS 430 reports. The discrepancy between field width measured by CI and that determined by film measurement is equal to or less than 2 mm, which is within the tolerance recommend by the guidelines of linac quality assurance in regular open FF photon fields, SPFs, and REFs with cone size of 10 × 10 cm in area. CI can be used to quantitatively measure the OF and profile of surface dose. It is feasible to use CI to measure the surface dose profile and field width in regular open FF photon fields and SPFs except for 1 × 1 cm field.
Topics: Electrons; Humans; Particle Accelerators; Phantoms, Imaging; Photons; Radiometry; Radiotherapy Dosage; Radiotherapy Planning, Computer-Assisted
PubMed: 35119327
DOI: 10.1177/15330338211073432 -
Physical Review Letters Jun 2022Reciprocity is a fundamental symmetry of Maxwell's equations. It is known that reciprocity imposes constraints on transmission, absorption, and emission. Here, we reveal...
Reciprocity is a fundamental symmetry of Maxwell's equations. It is known that reciprocity imposes constraints on transmission, absorption, and emission. Here, we reveal reciprocity constraints on reflection. We determine the sets of all attainable reflection coefficients of n-port scattering matrices with prescribed singular values, both with and without assuming reciprocity. Their difference establishes reciprocity constraints and nonreciprocal behaviors. As an application, we examine the conditions for all-zero reflections. Our results deepen the understanding of reciprocity in optics.
Topics: Optics and Photonics
PubMed: 35802447
DOI: 10.1103/PhysRevLett.128.256101 -
Scientific Reports Nov 2020Miniaturization and integration of optical tweezers are attractive. Optical fiber-based trapping systems allow optical traps to be realized in miniature systems, but the...
Miniaturization and integration of optical tweezers are attractive. Optical fiber-based trapping systems allow optical traps to be realized in miniature systems, but the optical traps in these systems lack reliability or mobility. Here, we present the all-fiber modular optical tweezers (AFMOTs), in which an optical trap can be reliably created and freely moved on a sample substrate. Two inclined optical fibers are permanently fixed to a common board, rendering a modular system where fiber alignments are maintained over months. The freely movable optical trap allows particles to be trapped in their native locations. As a demonstration, we applied AFMOTs to trap and deform freely floating individual cells. By the cell mechanical responses, we differentiated the nontumorigenic breast epithelial cell line (MCF10A) from its cancerous PTEN mutants (MCF10 PTEN-/-). To further expand the functionalities, three modalities of AFMOTs are demonstrated by changing the types of fibers for both the optical trap creation and particle position detection. As a miniature and modular system that creates a reliable and mobile optical trap, AFMOTs can find potential applications ranging from point-of-care diagnostics to education, as well as helping transition the optical trapping technology from the research lab to the field.
Topics: Breast; Breast Neoplasms; Cells, Cultured; Equipment Design; Female; Fiber Optic Technology; Humans; Optical Tweezers; Optics and Photonics
PubMed: 33208851
DOI: 10.1038/s41598-020-77067-1