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Australian Endodontic Journal : the... Sep 2023Successful root canal treatment requires effective irrigation of the entire root canal system. While chelating agents support irrigation, they can also alter...
Successful root canal treatment requires effective irrigation of the entire root canal system. While chelating agents support irrigation, they can also alter physicochemical properties of the root dentin structure. The aim of this study is to evaluate the effect of different chelation agents on root dentin roughness. Twenty-five extracted maxillary incisors were used in this study. Samples were separated longitudinally and divided into five groups: distilled water, NaOCl, ethylenediaminetetraacetic acid (EDTA), phytic acid and citric acid (CA). Atomic force microscope and energy dispersive X-ray spectroscopy analyses were used for analysing. One-way analysis of variance and Turkey tests were used in the statistical analysis of the study. EDTA, CA and phytic acid solutions increased the roughness and phytic acid and CA solutions decreased the Ca/P ratio in dentin tissue. No statistical difference was observed in the other groups. The decrease of Ca/P ratio should be taken under consideration during irrigation. The increase in surface roughness may provide clinical benefit by supporting the adhesion of the root canal filling materials to the dentin surface.
Topics: Edetic Acid; Phytic Acid; Sodium Hypochlorite; Dentin; Chelating Agents; Dental Pulp Cavity; Citric Acid; Root Canal Irrigants
PubMed: 36116094
DOI: 10.1111/aej.12691 -
Biotechnology and Applied Biochemistry Oct 2023Phosphorous actively participates in numerous metabolic and regulatory activities of almost all living organisms including animals and humans. Therefore, it is... (Review)
Review
Phosphorous actively participates in numerous metabolic and regulatory activities of almost all living organisms including animals and humans. Therefore, it is considered as an essential macronutrient required supporting their proper growth. On contrary, phytic acid (PA), an antinutritional substance, is widely known for its strong affinity to chelate essential mineral ions including PO , Ca , Fe , Mg , and Zn . Being one the major reservoir of PO ions, PA has great potential to bind PO ions in diverse range of foods. Once combined with P, PA transforms into an undigested and insoluble complex namely phytate. Produced phytate leads to a notable reduction in the bioavailability of P due to negligible activity of phytases in monogastric animals and humans. This highlights the importance and consequent need of enhancement of phytase level in these life forms. Interestingly, phytases, catalyzing the breakdown of phytate complex and recycling the phosphate into ecosystem to its available form, have naturally been reported in a variety of plants and microorganisms over past few decades. In pursuit of a reliable solution, the focus of this review is to explore the keynote potential of bacterial phytases for sustainable management of phosphorous via efficient utilization of soil phytate. The core of the review covers detailed discussion on bacterial phytases along with their widely reported applications viz. biofertilizers, phosphorus acquisition, and plant growth promotion. Moreover, meticulous description on fermentation-based strategies and future trends on bacterial phytases have also been included.
Topics: Humans; Animals; Phytic Acid; 6-Phytase; Ecosystem; Phosphorus; Phosphates
PubMed: 37042496
DOI: 10.1002/bab.2466 -
International Journal of Pharmaceutics Jan 2022The preferable choice of sustained peptide delivery systems is generally polymer-based microspheres in which their large particle size, wide size distribution, low drug...
The preferable choice of sustained peptide delivery systems is generally polymer-based microspheres in which their large particle size, wide size distribution, low drug encapsulation efficacy, poor colloidal stability, and undesirable burst release eventually hinder their clinical translation. In this study, a nanoscale ternary Lixisenatide (Lix) sustained delivery system based on strong multivalent interactions (electrostatic and coordination complexation) among small molecular phytic acid (PA), Lix and Fe was developed. Flash nanocomplexation (FNC) was utilized to facilitate the rapid and efficient mixing of the three components and kinetically control the assembly process that enabled dynamic balance of two competitive chemical reactions with different kinetic rates (slow chemical reaction of PA/Lix and fast chemical reaction of PA/Fe) to generate structural uniform ternary nanoparticles and avoid heterogeneous complexes. By tuning the mixing conditions (i.e., flow rate, mass ratio, concentration, pH value, etc.), the ternary PA/Lix/Fe nanoparticles were assembled with reproducible production in a manner of high uniformity and scalability, achieving small size (∼50 nm), uniform composition (PDI: ∼0.12), favourable colloidal stability, high encapsulation efficiency (∼100%), and tunable drug release kinetics. The optimized formulation exhibited a minor Lix release (<20%) in the first day and extended peptide release period over 8 days. Unexpectedly, upon a single injection administration, the as-prepared formulation (600 μg/kg) rapidly brought the high BGL (∼30 mmol/L) back to normal range (<10 mmol/L) within the initial 6 h and achieved a 180 h glycemic control in T2D mouse model. Moreover, this sustained peptide delivery system demonstrated a repeatable hypoglycemic effects and significantly suppressed the pathological damage of major organs following multiple injection. This sustained peptide delivery system with aqueous, facile and reproducible preparation process possesses good biocompatibility, tunable release kinetics, and prolonged hypoglycemic effects, portending its great translational potential in the chronic disease treatment.
Topics: Animals; Kinetics; Mice; Nanoparticles; Peptides; Phytic Acid
PubMed: 34838624
DOI: 10.1016/j.ijpharm.2021.121317 -
Phosphorylation and citration of normal corn starch by dry heating with phytic acid and citric acid.International Journal of Biological... Jan 2023Normal corn starch was subjected to dry heating in the presence of phytic acid (PA, 2 %, starch basis) and citric acid (CA, 5 % and 10 %) for modification. Dual...
Normal corn starch was subjected to dry heating in the presence of phytic acid (PA, 2 %, starch basis) and citric acid (CA, 5 % and 10 %) for modification. Dual treatment with PA and CA induced structural and physicochemical changes in normal corn starch. Phosphorus concentration, degree of substitution, FTIR, and P NMR analyses confirmed esterification of starch by dry heating with PA and CA. Both phosphorylation and citration by esterification with PA and CA were observed in PA + 5CA starch, but high CA concentration inhibited covalent interaction between PA and starch in PA + 10CA starch. The degree of phosphorylation and citration resulted in different physicochemical properties in starch treated with PA and CA. The treatment with only PA did not change the crystalline regions of PA starch, but CA treatment induced the disruption of the crystalline structure of PA + 5CA and PA + 10CA starch. PA starch showed high solubility (46.41 %) and transmittance (90.51 %), but dual treatment of PA and CA induced significant decrease in solubility (3.23 %) and transmittance (2.18 %) of PA + 10CA starch. CA treatment increased the fraction of resistant starch in non-cooked (72.44 %) and cooked PA + 10CA starch (42.76 %). Therefore, dual treatment with PA and CA had potential to control physicochemical and functional properties of starch by phosphorylation and citration of starch.
Topics: Starch; Phytic Acid; Zea mays; Citric Acid; Phosphorylation; Heating
PubMed: 36502943
DOI: 10.1016/j.ijbiomac.2022.12.028 -
Journal of Cosmetic Dermatology Mar 2021Improvement in skin luminosity and dyschromia such as postinflammatory hyperpigmentation (PIH) and actinic photodamage are leading causes for cosmetic consultation....
BACKGROUND
Improvement in skin luminosity and dyschromia such as postinflammatory hyperpigmentation (PIH) and actinic photodamage are leading causes for cosmetic consultation. Formulation of topical at home treatment is challenging, using a range of modalities, to help hinder or prevent inflammatory mediators without further irritating the skin. Glycolic acid is a known antioxidant; in various free acid levels, it has been introduced as a topical therapy aimed at reducing pigmentation and improving skin texture, tone, and luminosity.
METHODS
In a 12-week clinical study, a novel, topical facial emulsion containing 10% glycolic acid, 2% phytic acid, and soothing complex in emulsion was evaluated for its effectiveness in treating skin quality in American female volunteers with Fitzpatrick skin types II-VI. Efficacy evaluations were performed at pretreatment baseline, weeks 2, 4, 8, and 12, and included expert clinical grading, and self-assessment questionnaires. Cutaneous tolerability was also evaluated by assessing subjective and objective irritation of the treatment area.
RESULTS
Significant improvement in the appearance of skin PIH, hyperpigmentation, texture, and tone homogeneity was observed beginning at week 4 and continued through week 12.
CONCLUSIONS
The findings suggest that the test product is well suited for at-home skincare. It was both well-tolerated and an effective treatment option for addressing hyperpigmentation and overall skin luminosity.
Topics: Emulsions; Female; Glycolates; Humans; Hyperpigmentation; Phytic Acid; Treatment Outcome
PubMed: 33458927
DOI: 10.1111/jocd.13950 -
Enzyme and Microbial Technology May 2021A simple, convenient and efficient enzyme immobilization method through phytic acid (PA) modified α-Glucosidase (α-Glu)/Cu(PO)·3HO hybrid nanoflower was developed....
A simple, convenient and efficient enzyme immobilization method through phytic acid (PA) modified α-Glucosidase (α-Glu)/Cu(PO)·3HO hybrid nanoflower was developed. The structural properties of the materials were studied by several characterization techniques. Subsequently, the enzymatic reaction conditions such as the pH value and temperature were optimized, and the enzyme kinetics and inhibition parameters were determined. The PA modified α-Glu/Cu(PO)·3HO hybrid nanoflower had better enzymatic activity under a wide pH range and high temperature than the free one. After seven successive cycles, the PA modified α-Glu/Cu(PO)·3HO hybrid nanoflower could still maintain approximately 63.0 % of its initial immobilized enzyme activity. The Michaelis-Menten constant (K) and the half-maximal inhibitory concentration (IC) of acarbose were determined as 0.77 mM and 15.01 μM, respectively. In addition, the material was applied to evaluate the inhibitory activity of ten phenolic compounds on α-Glu, and epicatechin gallate, gallocatechin gallate, epigallocatechin gallate and rosmarinic acid showed good inhibitory activity with % of inhibition of (53.42 ± 2.39)%, (37.28 ± 1.32)%, (37.08 ± 0.63)% and (35.53 ± 0.23)%, respectively. These results indicate that the PA modified hybrid nanoflower is an efficient method of α-Glu immobilization.
Topics: Enzymes, Immobilized; Glucosidases; Phytic Acid; Temperature
PubMed: 33812564
DOI: 10.1016/j.enzmictec.2021.109776 -
Journal of Materials Chemistry. B Feb 2021Highly specific enrichment of phosphopeptides from complex biological samples was a precondition for further studying its physiological and pathological processes due to...
Highly specific enrichment of phosphopeptides from complex biological samples was a precondition for further studying its physiological and pathological processes due to the important and trace amounts of phosphopeptides. In this work, phytic acid (PA) functionalized magnetic cerium and zirconium bimetallic metal-organic framework nanocomposites (denoted as Fe3O4@SiO2@Ce-Zr-MOF@PA) were fabricated by a facile yet efficient method. The as-prepared nanomaterial exhibited high sensitivity (0.1 fmol μL-1), high selectivity toward phosphopeptides from β-casein tryptic digests/BSA (1 : 800), and good reusability of five cycles for enriching phosphopeptides. This affinity probe was applied to biological samples, and 19, 4 and 15 phosphopeptides were identified from non-fat milk, human serum and human saliva, respectively. The above marked advantages are attributed to the strong affinity of the abundant Ce-O and Zr-O nanoclusters on the surface of the MOF shell with the improved hydrophilicity from a great number of phosphate groups. Therefore, the novel Fe3O4@SiO2@Ce-Zr-MOF@PA nanospheres could not only enrich phosphopeptides effectively, but also reduce the adsorption of phosphopeptides, manifesting great potential in the identification and further analysis of low abundance phosphopeptides in complex biological samples.
Topics: Biomarkers; Humans; Metal-Organic Frameworks; Molecular Conformation; Particle Size; Phosphopeptides; Phytic Acid; Surface Properties
PubMed: 33503098
DOI: 10.1039/d0tb02517h -
Journal of Materials Science. Materials... Jun 2024Calcium phosphate cements, primarily brushite cements, require the addition of setting retarders to ensure adequate processing time and processability. So far, citric...
Calcium phosphate cements, primarily brushite cements, require the addition of setting retarders to ensure adequate processing time and processability. So far, citric acid has been the primary setting retarder used in this context. Due to the poor biocompatibility, it is crucial to explore alternative options for better processing. In recent years, the setting retarder phytic acid (IP6) has been increasingly investigated. This study investigates the biological behaviour of calcium phosphate cements with varying concentrations of IP6, in addition to their physical properties. Therefore cytocompatibility in vitro testing was performed using osteoblastic (MG-63) and osteoclastic (RAW 264.7 differentiated with RANKL) cells. We could demonstrate that the physical properties like the compressive strength of specimens formed with IP6 (brushite_IP6_5 = 11.2 MPa) were improved compared to the reference (brushite = 9.8 MPa). In osteoblast and osteoclast assays, IP6 exhibited significantly better cytocompatibility in terms of cell activity and cell number for brushite cements up to 11 times compared to the brushite reference. In contrast, the calcium-deficient hydroxyapatite (CDHA) cements produced similar results for IP6 (CDHA_IP6_0.25 = 27.0 MPa) when compared to their reference (CDHA = 21.2 MPa). Interestingly, lower doses of IP6 were found to be more effective than higher doses with up to 3 times higher. Additionally, IP6 significantly increased degradation in both passive and active resorption. For these reasons, IP6 is emerging as a strong new competitor to established setting retarders such as citric acid. These cements have potential applications in bone augmentation, the stabilisation of non-load bearing fractures (craniofacial), or the cementation of metal implants.
Topics: Phytic Acid; Animals; Calcium Phosphates; Mice; Materials Testing; Bone Cements; Osteoblasts; RAW 264.7 Cells; Humans; Osteoclasts; Compressive Strength; Biocompatible Materials; Durapatite
PubMed: 38900219
DOI: 10.1007/s10856-024-06805-y -
Biochemistry. Biokhimiia Jan 2021The release of phosphorus from phytates occurs via sequential cleavage of phosphate groups. It was believed that, regardless of the properties of phytases, the rate of... (Review)
Review
The release of phosphorus from phytates occurs via sequential cleavage of phosphate groups. It was believed that, regardless of the properties of phytases, the rate of phytate dephosphorylation is limited by the first cleavage of any phosphate group. The position of the first cleaved-off phosphate group depending on the specificity of phytase. The inhibition of dephosphorylation initiation is not associated with the action mechanism of the enzyme and can be rather due to the insufficient phytase activity or low availability of phytates. The analysis of the transformations in the inositol hexakisphosphate (IP)→inositol (I) reaction chain shows that IP dephosphorylation as a whole limits the phosphate group removal from I(1,2,5,6)P (third reaction from the beginning of hydrolysis of phosphate bonds in PA). The lower availability of nutrients in the presence of phytates is not due to action of phytates, but is caused by PA anions (IP), which bind positively charged metal ions, amino acids, and proteins. The availability of nutrients increases as a result of the decrease in their binding caused by the decrease in the concentration of IP anions under the action of phytases. Phytases added to feeds play a lesser role in the digestion of phytates compared to natural enzymes and complement their action. The concept of extra-phosphoric effect has no scientific justification, since phytases exhibit only the phosphohydrolase activity and are not able to catalyze other reactions.
Topics: 6-Phytase; Animal Nutritional Physiological Phenomena; Animals; Phytic Acid
PubMed: 33827406
DOI: 10.1134/S000629792114011X -
Fish & Shellfish Immunology Sep 2019Phytic acid (PA) is one of the most common anti-nutritional factors in plant-derived protein feeds, and it poses considerable threats to aquaculture production. However,...
Phytic acid (PA) is one of the most common anti-nutritional factors in plant-derived protein feeds, and it poses considerable threats to aquaculture production. However, little is known about the effects of PA on fish intestinal health. This study aimed to investigate the impacts of PA on intestinal immune function in on-growing grass carp. To achieve this goal, a growth trial was conducted for 60 days by feeding 540 fish (120.56 ± 0.51 g) with six semi-purified diets containing graded levels of PA (0, 0.8, 1.6, 2.4, 3.2 and 4.0%). Then fish were challenged with Aeromonas hydrophila for 6 days. The results indicated that, compared with the control group (0% PA), PA did the following: (1) suppressed fish growth performance (percentage weight gain and feed efficiency) and reduced their ability to resist enteritis; (2) decreased fish intestinal antimicrobial ability by reducing intestinal lysozyme (LZ) activities, the contents of complement 3 (C3), C4 and immunoglobulin M (IgM), and downregulating the mRNA levels of hepcidin, liver-expressed antimicrobial peptide 2A (LEAP-2A), LEAP-2B, and β-defensin-1; and (3) aggravated fish intestinal inflammation responses by upregulating the mRNA levels of pro-inflammatory cytokines including tumour necrosis factor α (TNF-α), interleukin 1β (IL-1β) (except in the DI), interferon γ2 (IFN-γ2), IL-8, IL-12p40, IL-15 (except in the DI) and IL-17D, which is partly related to the nuclear factor kappa B (NF-κB) signalling pathway, whereas downregulating the mRNA levels of anti-inflammatory cytokines including transforming growth factor β1 (TGF-β1), IL-4/13A, IL-4/13B, IL-10 and IL-11, which is partially associated with the target of rapamycin (TOR) signalling pathway. The possible reasons for some distinctive gene expression patterns in fish three intestinal segments were discussed. Finally, based on the percent weight gain, enteritis morbidity, IgM content and LZ activity in the PI, the maximum tolerance levels of PA for on-growing grass carp were estimated to be 2.17, 1.68, 1.47 and 1.18% of the diet, respectively.
Topics: Adaptive Immunity; Aeromonas hydrophila; Animal Feed; Animals; Carps; Diet; Dietary Supplements; Dose-Response Relationship, Drug; Fish Diseases; Gram-Negative Bacterial Infections; Immunity, Innate; Intestines; Phytic Acid; Random Allocation
PubMed: 31247320
DOI: 10.1016/j.fsi.2019.06.045