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Critical Reviews in Food Science and... 2022Isolation and utilization of proteins from seaweeds have been a novel trend in the world at present due to the increasing demand for healthy non-animal proteins. The... (Review)
Review
Isolation and utilization of proteins from seaweeds have been a novel trend in the world at present due to the increasing demand for healthy non-animal proteins. The attention of scientific community has been paid on the protein derived from seaweed due to their high nutritional quality and bioactivity. This article aims to provide an integrated overview on methods of extraction, isolation and purification of . derived proteins and composition, nutritional value and potential nutraceutical and food applications with an interest to stimulate further research to optimize the utilization. Potential food applications of . derived proteins are nutritional components in human diet, food ingredients and additives, alternative meat and meat analogues and animal and fish feed. Excellent antioxidant, antihypertension, anticoagulant, anti-diabetes, antimicrobial and anti-cancer activities possessed by proteins of . enable the use of these proteins in various nutraceutical applications. A number of studies have been carried out on antioxidant and antihypertensive activities of . proteins, whereas other bioactivites are yet to be further studied. Hence, more research works are crucial to be done in order to facilitate and promote the emerging novel foods and nutraceuticals, using proteins from seaweed . .
Topics: Animals; Dietary Supplements; Humans; Plant Proteins; Plant Proteins, Dietary; Seaweed; Undaria
PubMed: 33703974
DOI: 10.1080/10408398.2021.1898334 -
Plant Physiology and Biochemistry : PPB Feb 2021VQ proteins are a class of plant-specific proteins containing the conserved motif FxxhVQxhTG(h denotes hydrophobic residues and x represents any amino acid)and are... (Review)
Review
VQ proteins are a class of plant-specific proteins containing the conserved motif FxxhVQxhTG(h denotes hydrophobic residues and x represents any amino acid)and are named VQ for the V and Q residues. By analyzing the structure of VQ members it was found that most VQ genes do not contain introns and the number of encoded amino acids is less than 300 aa. A majority of VQ proteins are located in the nucleus. Accumulated evidence has highlighted the importance of VQ proteins mainly participating in signal pathways through interacting with partners (eg. WRKYs and MAPKs) to regulate plant growth and development and respond to biotic and abiotic stresses. This review primarily focuses on the structure of VQ members in plant kingdom and the biological function and the mechanism of VQ protein action, and discusses recent advances in understanding the pivotal role of VQ-motif, which provides a solid foundation for further exploration on VQ proteins.
Topics: Amino Acid Motifs; Gene Expression Regulation, Plant; Plant Proteins; Plants; Signal Transduction; Stress, Physiological
PubMed: 33310402
DOI: 10.1016/j.plaphy.2020.12.005 -
Current Protein & Peptide Science 2021Many unfavorable stress conditions, such as wounding, drought, extreme temperatures, salinity and pathogen attacks, control growth, development and plant yield. To... (Review)
Review
Many unfavorable stress conditions, such as wounding, drought, extreme temperatures, salinity and pathogen attacks, control growth, development and plant yield. To survive in such environments, plants have developed many strategies. They are able to induce the expression of a large number of genes that encode effectors, receptors, as well as signaling proteins and protective molecules. Among all, pathogenesis-related proteins (PRs) were found to be activated in response to different biotic and abiotic threats. Those proteins have a wide range of functions; acting as chitinases, peroxidases, anti-microbial agents, hydrolases, protease inhibitors, and other activities. Activation of PR proteins has been demonstrated in different plant families as a response to different stresses. In this review, we have summarized the structural, biological and functional characteristics of the different PRs families in plants, their regulation, as well as their roles in plant defense against abiotic and biotic stresses.
Topics: Gene Expression Regulation, Plant; Plant Diseases; Plant Proteins; Stress, Physiological
PubMed: 33390143
DOI: 10.2174/1389203721999201231212736 -
Peptides Aug 2021Plant protein-derived peptides, focusing especially on soybean protein-derived peptides have considerable effects on metabolic regulation and modulation such as... (Review)
Review
Plant protein-derived peptides, focusing especially on soybean protein-derived peptides have considerable effects on metabolic regulation and modulation such as cholesterol lowering, triglyceride lowering, anti-obesity, inhibition of fatty acid synthase, and antidiabetic effects. The molecules targeted to study the metabolic regulatory functions of the peptides included the following: intestinal cholesterol micelle, cholesterol metabolism-related genes for cholesterol lowering, triglyceride metabolism-related genes for triglyceride lowering and anti-obesity, dipeptidyl peptidase-IV (DPP-IV), α-amylase, α-glucosidase, or glucose metabolism-related genes for lowering blood glucose levels. This review article outlines the physiological functions of plant protein-derived peptides for the improvement of lipid and glucose metabolism in vitro or in vivo.
Topics: Glucose; Lipid Metabolism; Peptide Fragments; Plant Proteins
PubMed: 34033874
DOI: 10.1016/j.peptides.2021.170577 -
Plant Molecular Biology Feb 2022An overview is presented of recent advances in our knowledge of candidate proteins that regulate various physiological and biochemical processes underpinning plant... (Review)
Review
An overview is presented of recent advances in our knowledge of candidate proteins that regulate various physiological and biochemical processes underpinning plant adaptation to saline conditions. Salt stress is one of the environmental constraints that restrict plant distribution, growth and yield in many parts of the world. Increased world population surely elevates food demands all over the globe, which anticipates to add a great challenge to humanity. These concerns have necessitated the scientists to understand and unmask the puzzle of plant salt tolerance mechanisms in order to utilize various strategies to develop salt tolerant crop plants. Salt tolerance is a complex trait involving alterations in physiological, biochemical, and molecular processes. These alterations are a result of genomic and proteomic complement readjustments that lead to tolerance mechanisms. Proteomics is a crucial molecular tool that indicates proteins expressed by the genome, and also identifies the functions of proteins accumulated in response to salt stress. Recently, proteomic studies have shed more light on a range of promising candidate proteins that regulate various processes rendering salt tolerance to plants. These proteins have been shown to be involved in photosynthesis and energy metabolism, ion homeostasis, gene transcription and protein biosynthesis, compatible solute production, hormone modulation, cell wall structure modification, cellular detoxification, membrane stabilization, and signal transduction. These candidate salt responsive proteins can be therefore used in biotechnological approaches to improve tolerance of crop plants to salt conditions. In this review, we provided comprehensive updated information on the proteomic data of plants/genotypes contrasting in salt tolerance in response to salt stress. The roles of salt responsive proteins that are potential determinants for plant salt adaptation are discussed. The relationship between changes in proteome composition and abundance, and alterations observed in physiological and biochemical features associated with salt tolerance are also addressed.
Topics: Adaptation, Physiological; Gene Expression Regulation, Plant; Plant Proteins; Plants; Salt Stress; Sodium Chloride
PubMed: 34964081
DOI: 10.1007/s11103-021-01232-x -
International Journal of Molecular... Jan 2022Cold stress limits plant geographical distribution and influences plant growth, development, and yields. Plants as sessile organisms have evolved complex biochemical and... (Review)
Review
Cold stress limits plant geographical distribution and influences plant growth, development, and yields. Plants as sessile organisms have evolved complex biochemical and physiological mechanisms to adapt to cold stress. These mechanisms are regulated by a series of transcription factors and proteins for efficient cold stress acclimation. It has been established that the signaling pathway in plants regulates how plants acclimatize to cold stress. Cold stress is perceived by receptor proteins, triggering signal transduction, and () genes are activated and regulated, consequently upregulating the transcription and expression of the () genes. The protein binds to the (), a homeopathic element of the genes ( gene) promoter, activating their transcription. Transcriptional regulations and post-translational modifications regulate and modify these entities at different response levels by altering their expression or activities in the signaling cascade. These activities then lead to efficient cold stress tolerance. This paper contains a concise summary of the pathway elucidating on the cross interconnections with other repressors, inhibitors, and activators to induce cold stress acclimation in plants.
Topics: Cold-Shock Response; Gene Expression Regulation, Plant; Plant Physiological Phenomena; Plant Proteins; Protein Processing, Post-Translational; Signal Transduction; Trans-Activators; Transcriptional Activation
PubMed: 35163471
DOI: 10.3390/ijms23031549 -
Plant Science : An International... Oct 2023Inositolphosphorylceramide synthase (IPCS) catalyses ceramides and phosphatidylinositol (PI) into inositolphosphorylceramide (IPC), which is involved in the regulation...
Inositolphosphorylceramide synthase (IPCS) catalyses ceramides and phosphatidylinositol (PI) into inositolphosphorylceramide (IPC), which is involved in the regulation of plant growth and development. A total of three OsIPCS family genes have been identified in rice. However, most of their functions remain unknown. Here, the functions of OsIPCSs were analyzed by CRISPR/Cas9 technology, lipidomics analysis, and transcriptomics analysis. Single-gene mutation of OsIPCSs resulted in dwarf phenotype. Among them, the phenotype of osipcs3 mutant was more severe. Multi-gene mutation of OsIPCS genes led to more severe phenotypes, indicating the additive effects of OsIPCSs. We further determined that a significant decrease in epidermal cell elongation of internode in the mutants. There was a significant decrease in the content of IPC detected in the osipcs2/3 and osipcs1/2/3 mutants. The contents of glycosyl inositol phosphoryl ceramide (GIPC) were also decreased by 20% and 10% in osipcs2/3 and osipcs1/2/3, respectively. The results of RNA-seq showed that numerous DEGs found to be associated with cellular component organization, anatomical structure morphogenesis, and cell growth in the osipcs2, osipcs2/3, and osipcs1/2/3. Taken together, OsIPCSs may be involved in the regulation of plant height through affecting cell growth and sphingolipid metabolism in rice.
Topics: Oryza; Mutation; Glycosphingolipids; Ceramides; Gene Expression Regulation, Plant; Plant Proteins; Phenotype
PubMed: 37467787
DOI: 10.1016/j.plantsci.2023.111798 -
The Protein Journal Aug 2020Antifungal proteins and peptides have drawn the attention of numerous plant biologists and Clinicians, owing to their potential value in protecting commercial crops as...
Antifungal proteins and peptides have drawn the attention of numerous plant biologists and Clinicians, owing to their potential value in protecting commercial crops as well as preventing fungal infections in humans. Various proteins and peptides, such as glucanases, chitinases, chitinase-like proteins, lectins, peroxidases, defensins, and lipid transfer proteins have antifungal activities. Thaumatin is a protein from a West African plant Thaumatococcus danielli that is sweet in taste but does not exhibit antifungal activities. Despite the structural similarities between thaumatins and thaumatin-like proteins (TLPs), TLPs are not sweet in taste, unlike thaumatins. We developed a thaumatin-like protein database of various organisms. TLPs are pathogenesis-related proteins (PR) with molecular masses of 20-26 kDa. The amino acid residues of TLPs involved in an antifungal activity remain obscure and make it hard to receive comprehensive information on TLPs. The biggest problem in the wine industry is white haze, an undesirable feature of high-quality wine. Hence, the problem may be figured out with the easy accessibility of amino acid sequences and to generate infest resistant crops. Overall, we aimed to produce a freely accessible TLP database ( https://tlpdb.cftri.com ) that would provide substantive information in understanding the mechanistic facet of TLPs. Briefly, TLPdb contains sequences, structures, and amino acid compositions of validated, published TLP protein sequences (from the plant, fungal as well as animal sources). Thus, this work may yield valuable information that may be useful in understanding the mechanistic aspects of TLP activity and in the evolution of antifungal proteins and fungal resistant crops. TLPdb is a comprehensive thaumatin-like protein resource database of various organisms. The database can serve as a unique Bioinformatics tool for understanding the TLPs. This further may help in understanding and the development of fungal resistant crops. TLPdb is freely available at https://tlpdb.cftri.com .
Topics: Amino Acid Sequence; Databases, Protein; Plant Proteins; Sequence Homology, Amino Acid
PubMed: 32696292
DOI: 10.1007/s10930-020-09909-w -
International Journal of Molecular... Mar 2020Lateral organ boundaries (LOB) domain () genes, a gene family encoding plant-specific transcription factors, play important roles in plant growth and development. At... (Review)
Review
Lateral organ boundaries (LOB) domain () genes, a gene family encoding plant-specific transcription factors, play important roles in plant growth and development. At present, though there have been a number of genome-wide analyses on gene families and functional studies on individual LBD proteins, the diverse functions of LBD family members still confuse researchers and an effective strategy is required to summarize their functional diversity. To further integrate and improve our understanding of the phylogenetic classification, functional characteristics and regulatory mechanisms of LBD proteins, we review and discuss the functional characteristics of LBD proteins according to their classifications under a phylogenetic framework. It is proved that this strategy is effective in the anatomy of diverse functions of LBD family members. Additionally, by phylogenetic analysis, one monocot-specific and one eudicot-specific subclade of LBD proteins were found and their biological significance in monocot and eudicot development were also discussed separately. The review will help us better understand the functional diversity of LBD proteins and facilitate further studies on this plant-specific transcription factor family.
Topics: Conserved Sequence; Phylogeny; Plant Proteins; Protein Domains; Transcription Factors
PubMed: 32224847
DOI: 10.3390/ijms21072278 -
Peptides Oct 2021Plants have evolved diverse peptide hormones and cognate receptors to orchestrate plant growth and development. Secreted peptide ligands are mainly sensed by membrane... (Review)
Review
Plants have evolved diverse peptide hormones and cognate receptors to orchestrate plant growth and development. Secreted peptide ligands are mainly sensed by membrane receptor kinases that mediate cell-cell communication. The secreted peptides are categorized into two groups: small linear post-translationally modified peptides and cysteine-rich peptides. The small linear peptides are recognized by the corresponding receptors and co-receptors in a conserved manner. By contrast, the cysteine-rich peptides are perceived by various types of receptor proteins using diverse binding modes. Recent studies have revealed the molecular and mechanistic origins of peptide recognition and receptor activation. This review summarizes plant-peptide binding modes and receptor-activation mechanisms that have been structurally characterized in recent studies.
Topics: Peptides; Plant Proteins; Protein Kinases; Receptors, Cell Surface
PubMed: 34332962
DOI: 10.1016/j.peptides.2021.170614