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Biological Trace Element Research Nov 2022The aim of the present study was to evaluate the arsenic-induced hemotoxicity and to evaluate the protective effects of Lactobacillus sporogenes in male albino Wistar...
The aim of the present study was to evaluate the arsenic-induced hemotoxicity and to evaluate the protective effects of Lactobacillus sporogenes in male albino Wistar rats. A total of 36 adult male albino Wistar rats were procured and divided into 3 groups of 12 animals each. Group 1 rats served as control, group 2 rats were administered sodium arsenite (@5 mg/kg BW/day), groups 3 rats were supplemented with L. sporogenes (@15 million spores/kg BW/day) along with sodium arsenite administered along with sodium arsenite orally daily for 28 consecutive days. Weekly body weights, hematological profile, and erythrocyte morphology were assessed. Significant (P < 0.05) reduction in mean weekly body weights (g) was observed in group 2 than group 1; however, a significant (P < 0.05) increase in weekly body weights was observed in group 3 as compared to group 2. A significant (P < 0.05) decrease in erythrocyte-related parameters and platelet counts, and a significant (P < 0.05) leukocytosis, relative lymphopenia, absolute neutrophilia, and monocytosis were noticed among arsenic-treated rats when compared to the control group. Blood smear of arsenic-treated rats contains echinocytes, microcytes, and spherocytes when compared to control. Scanning electron microscopic examination of blood revealed altered erythrocyte morphology in arsenic-treated rats with poikilocytosis and blebbing of the erythrocyte membrane. Supplementation of L. sporogenes along with arsenic resulted in improvement of all the hematological parameters and reduction in morphological abnormalities in comparison to the toxic control group. It is concluded that supplementation of L. sporogenes can effectively alleviate the arsenic-induced hematological alterations.
Topics: Animals; Arsenic; Arsenites; Bacillus coagulans; Body Weight; Male; Oxidative Stress; Rats; Rats, Wistar; Sodium Compounds
PubMed: 34993908
DOI: 10.1007/s12011-021-03055-9 -
Cureus Mar 2022Sickle cell disease is an autosomal recessive disorder resulting in the substitution of CTG by CAG in the sixth codon of the beta-globin gene. As a result of this, the...
Sickle cell disease is an autosomal recessive disorder resulting in the substitution of CTG by CAG in the sixth codon of the beta-globin gene. As a result of this, the hydrophilic glutamic acid residue is replaced by hydrophobic valine residue, leading to the formation of hemoglobin tetramer HBS. This alteration in the beta-globin chain makes the red blood cells prone to sickling, especially in the presence of risk factors such as stress, hypoxia, and infection. These sickled red blood cells have the tendency to adhere to the endothelium and lead to vessel occlusion and distal tissue ischemia. The recent coronavirus disease 2019 (COVID-19) outbreak has impacted millions across the globe, putting individuals with co-morbidities at particularly high risk, and patients with sickle cell disease are no exception. We present the case of a 47-year-old African American male presenting to the emergency department with subjective fevers and a two-day history of pain in the arms, legs, and chest. A diagnosed case of sickle cell disease, the patient was on hydromorphone for pain management but ran out of his medications a few weeks before presentation. On examination, the patient was saturating well with mild tenderness upon palpation of the arms, legs, and chest. On complete blood count, the patient had a hemoglobin of 11.3 g/dL and a white cell count of 13.1 x10(3)/mcL. The patient had a normal mean corpuscular volume with reticulocytosis, hypochromia, ovalocytosis, poikilocytosis, polychromasia, and target cells. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) was positive. The chest X-ray did not reveal any significant findings. He was admitted to the medicine floor for the management of sickle cell crisis and was placed under airborne and droplet precautions. The patient was started on hydromorphone for pain management and intravenous fluid hydration. On the second day of admission, the patient reported increasing shortness of breath. He was saturating 90% on room air and 94% on 2 liters of supplemental oxygen. The white blood cell count increased to 18.42 x10(3)/mcL and the chest X-ray revealed reticular densities with patchy alveolar opacities in the left lung. Given the decline in respiratory status, the patient was started on remdesivir. Over the course of his hospital stay, the patient's pain and respiratory status improved, with the patient saturating 97% on room air. He was discharged home with instructions to follow isolation precautions for at least two weeks, folic acid, and adequate pain management. An appointment was also scheduled for the patient to follow with a sickle cell nurse practitioner upon discharge.
PubMed: 35494937
DOI: 10.7759/cureus.23604 -
Veterinary Research Communications Apr 2024South American camelids (SACs) play an increasing role in veterinary care in Europe. Many alpacas or llamas presented to veterinarians suffer from anaemia, regularly... (Review)
Review
South American camelids (SACs) play an increasing role in veterinary care in Europe. Many alpacas or llamas presented to veterinarians suffer from anaemia, regularly with a packed cell volume (PCV) below 0.10 l/l, which is a life-threatening condition for the animals. This review article presents clinical and laboratory diagnostic tools for the diagnosis of anaemia in SACs. Clinical identification of anaemic animals can be performed by assessing the FAMACHA© score and the Body Condition Score (BCS), since anaemia in alpacas and llamas correlates with pale mucous membranes and a lowered BCS. Haematological examination of a blood sample can provide a more differentiated diagnosis of anaemia in SACs. A common finding is regenerative anaemia with an increased number of reticulocytes that is often caused by blood loss due to Haemonchus contortus. Changes in a blood smear from an alpaca or llama with regenerative anaemia may include normoblasts (nucleated red blood cells), anisocytosis, poikilocytosis, polychromasia, Howell-Jolly bodies or basophilic stippling. Furthermore, non-regenerative anaemia, often caused by trace element deficiency or cachexia, can also occur.
Topics: Animals; Camelids, New World; Anemia; Haemonchus; South America
PubMed: 38049672
DOI: 10.1007/s11259-023-10274-z -
Journal of Clinical Pathology Mar 2023Recently, a new automated digital cell imaging analyser (Sysmex CellaVision DC-1), intended for use in low-volume and small satellite laboratories, has become available....
AIMS
Recently, a new automated digital cell imaging analyser (Sysmex CellaVision DC-1), intended for use in low-volume and small satellite laboratories, has become available. The purpose of this study was to compare the performance of the DC-1 with the Sysmex DI-60 system and the gold standard, manual microscopy.
METHODS
White blood cell (WBC) differential counts in 100 normal and 100 abnormal peripheral blood smears were compared between the DC-1, the DI-60 and manual microscopy to establish accuracy, within-run imprecision, clinical sensitivity and specificity. Moreover, the agreement between precharacterisation and postcharacterisation of red blood cell (RBC) morphological abnormalities was determined for the DC-1.
RESULTS
WBC preclassification and postclassification results of the DC-1 showed good correlation compared with DI-60 results and manual microscopy. In addition, the within-run SD of the DC-1 was below 1 for all five major WBC classes, indicating good reproducibility. Clinical sensitivity and specificity were, respectively, 96.7%/95.9% compared with the DI-60% and 96.6%/95.3% compared with manual microscopy. The overall agreement on RBC morphology between the precharacterisation and postcharacterisation results ranged from 49% (poikilocytosis) to 100% (hypochromasia, microcytosis and macrocytosis).
CONCLUSIONS
The DC-1 has proven to be an accurate digital cell imaging system for differential counting and morphological classification of WBCs and RBCs in peripheral blood smears. It is a compact and easily operated instrument that can offer low-volume and small satellite laboratories the possibilities of readily available blood cell analysis that can be stored and retrieved for consultation with remote locations.
Topics: Humans; Reproducibility of Results; Blood Cells; Leukocyte Count; Leukocytes; Hematologic Tests; Blood Cell Count
PubMed: 34620610
DOI: 10.1136/jclinpath-2021-207863 -
Transfusion Jan 2024Hereditary pyropoikilocytosis (HPP) is a heterogeneous inherited disorder of red blood cell (RBC) membrane and cytoskeletal proteins that leads to hemolytic anemia. HPP...
INTRODUCTION
Hereditary pyropoikilocytosis (HPP) is a heterogeneous inherited disorder of red blood cell (RBC) membrane and cytoskeletal proteins that leads to hemolytic anemia. HPP is characterized by marked poikilocytosis, microspherocytes, RBC fragmentation, and elliptocytes on peripheral blood smear. Mutations in SPTA1 can cause HPP due to a quantitative defect in α-spectrin and can lead to profound fetal anemia and nonimmune hydrops fetalis, which can be managed with intrauterine transfusion.
CASE PRESENTATION
We present a case of a 26-year-old G4P2102 woman of Amish-Mennonite ancestry with a pregnancy complicated by fetal homozygosity for an SPTA1 gene variant (SPTA1c.6154delG) as well as severe fetal anemia and hydrops fetalis, which was managed with four intrauterine transfusions between 26 and 30 weeks gestation. Pre-transfusion peripheral smears from fetal blood samples showed RBC morphology consistent with HPP. The neonate had severe hyperbilirubinemia at birth, which has resolved, but remains transfusion-dependent at 6 months of life.
DISCUSSION/CONCLUSION
To our knowledge, this is the first report that correlates homozygosity of the SPTA1c.6154delG gene variant with RBC dysmorphology and establishes the diagnosis of HPP.
Topics: Pregnancy; Female; Infant, Newborn; Humans; Adult; Hydrops Fetalis; Elliptocytosis, Hereditary; Cytoskeletal Proteins; Anemia, Hemolytic; Fetal Diseases; Hematologic Diseases
PubMed: 38031483
DOI: 10.1111/trf.17617 -
Environmental Science and Pollution... Feb 2023The use of plant growth regulators has led to environmental contamination of water bodies that occur adjacent to agricultural areas. Some of these chemicals are...
The use of plant growth regulators has led to environmental contamination of water bodies that occur adjacent to agricultural areas. Some of these chemicals are bioactive, not only to plants, but also to non-target exposed biota, namely of the aquatic compartment. Previous work demonstrated the establishment of hepato- and nephrotoxic effects in juvenile tilapia (Oreochromis niloticus) exposed via aquatic media to gibberellic acid (GA), which is among the most used plant growth regulators, in agricultural practices. Here, we investigated the effect of GA on hematological indices, poikilocytosis, nuclear abnormalities, and genotoxic indices measured in Nile tilapia (Oreochromis niloticus), as well as the putative protective effects of dietary supplementation of Spirulina (Arthrospira platensis). Fish were evenly assorted into 5 groups: group I served as a control, and groups II-V were fed diets supplemented with Spirulina at rates of 0 g/kg, 5 g/kg, 20 g/kg, and 100 g/kg, respectively, for 2 months before being exposed to 150 mg/L GA. The results revealed that GA exposure decreased significantly all hematological indices (P < 0.05), except leucocytes and mean corpuscular hemoglobin concentration (MCHC), compared to the control group (P > 0.05). GA exposure increased significantly the percentage of nuclear abnormalities, altered erythrocytes and the percentages of tail DNA, compared to the control group (P < 0.05). Spirulina supplementation restored the hematological, poikilocytosis, nuclear abnormalities, and the percentages of tail DNA to near normal levels. The 100 g/kg SP treatment was the most effective in attaining such effect, showing concentration-dependency. The present study reinforces our findings of the toxicity of GA on O. niloticus and suggests that the addition of Spirulina to fish diet can mitigate the hemotoxic effects of GA.
Topics: Animals; Cichlids; Tilapia; Spirulina; Plant Growth Regulators; Dietary Supplements; Diet; Animal Feed
PubMed: 36346524
DOI: 10.1007/s11356-022-23844-6 -
The Science of the Total Environment Dec 2022Increases in human population lead to an increase in urban wastes, which could affect wildlife in several ways. Urban pollutants can affect erythrocytes of birds...
Increases in human population lead to an increase in urban wastes, which could affect wildlife in several ways. Urban pollutants can affect erythrocytes of birds generating morphological membrane and nuclear anomalies. The Kelp Gull (Larus dominicanus) is an opportunistic species, which take advantage of urban environments, thus being highly exposed to environmental pollution. In northeastern Patagonia, the dynamic of the waste management was transformed in the last decade and consequently, gulls changed their movements in response to changes in waste management systems. The food available to the seagulls went from being a mixture of urban/fishing discards until 2015, when this landfill closures, to being domestic urban offerings. In order of evaluating genotoxicity and changes in pollutants exposition due to these changes, we analyzed the frequencies of erythrocytes nuclear abnormalities and micronuclei (ENAs and MN respectively) in 58 blood smears from adults extracted during the non-breeding season in two periods in landfills with different waste compositions: a mixed landfill (ML) in 2013 before closure (n = 24) versus an urban landfill (UL) (n = 34) in 2021. We found that the Kelp Gull showed high values of abnormalities with an average of 151.5 /10,000 RBC in comparison with other seabird species. The bud and notched types of ENAs were the most prevalent abnormalities in both sites. We did not find significate differences in the overall abnormality frequency between sites, however we found significant higher frequencies in displaced and tailed types of ENAs in ML. We also found poikilocytosis, as seen previously in other animals exposed experimentally to pollutants such as metals and crude oil. Cellular abnormalities found in the Kelp Gull suggest an exposition of individuals to pollutants in foraging areas. The hemispheric distribution and the synanthropic characteristics of the species denote its importance as a suitable global monitor of genotoxicity.
Topics: Animals; Birds; Charadriiformes; Environmental Pollutants; Humans; Kelp; Petroleum
PubMed: 35964745
DOI: 10.1016/j.scitotenv.2022.157958 -
Annals of Medicine and Surgery (2012) Dec 2022Secondary thrombocytosis, also known as reactive thrombocytosis, is defined as an abnormal increase in platelet count as a result of another underlying medical or...
INTRODUCTION
Secondary thrombocytosis, also known as reactive thrombocytosis, is defined as an abnormal increase in platelet count as a result of another underlying medical or surgical condition. Once the medical cause of reactive thrombocytosis was determined, it could be treated. In this case, supportive treatment with no iron supplements for anemia and infection improved the case condition rapidly.
CASE PRESENTATION
we report a 20 years old Sudanese female who presented with high-grade fever, right iliac fossa pain, hyper pigmented macules on the tongue and a past history of undiagnosed anemia. Laboratory results showed platelets = 1007 × 10^3/μl, hemoglobin = 3.5 g/dl with low MCV, total WBC was also high = 14.9 × 10^3/μl. Peripheral blood picture showed anisocytosis and poikilocytosis, microcytic hypochromic RBCs associated with target cells, pencil cells, teardrops cells and polychromies cells and with leukocytosis and very high platelets in the film. Abdominal ultrasound showed evidence of pelvic inflammatory disease. After receiving supportive treatment, antibiotics and 3 units of blood the patient showed remarkable improvement and reduction in platelet count.
DISCUSSION
We discuss the mechanism of the reactive thrombocytosis state and the variable treatment options when accompanied with iron deficiency anemia.
CONCLUSION
Reactive thrombocytosis with extreme platelet count should always be considered in patients presented with severe iron deficiency anemia and infection. In this case report the high platelet count was reversed successfully after commencing antibiotics and blood transfusion although of the poor patient compliance and the poor investigations were obtained from the patient.
PubMed: 36582913
DOI: 10.1016/j.amsu.2022.104927 -
Environmental Science and Pollution... Jun 2021Copper has toxic effects in fish, whereas the cyanobacterium Spirulina reportedly has protective effects against metal toxicity in various animal species. The current...
Investigating the effects of copper sulfate and copper oxide nanoparticles in Nile tilapia (Oreochromis niloticus) using multiple biomarkers: the prophylactic role of Spirulina.
Copper has toxic effects in fish, whereas the cyanobacterium Spirulina reportedly has protective effects against metal toxicity in various animal species. The current study, therefore, aimed to investigate the prophylactic role of Spirulina platensis against the effects of copper sulfate (CuSO) and copper oxide nanoparticles (CuO-NPs) in Nile tilapia (Oreochromis niloticus). Biochemical, antioxidant, erthyron profile and histopathological endpoints were assessed after for 15 days of exposure in five separate treatment groups: (1) fish pre-fed the normal diet (control), (2) fish pre-fed the normal diet and exposed to 15 mg/L of CuSO, (3) fish pre-fed the normal diet augmented with 0.25% Spirulina and exposed to 15 mg/L of CuSO, (4) fish pre-fed the normal diet and exposed to 15 mg/L of CuO-NPs, and (5) fish pre-fed the normal diet augmented with 0.25% Spirulina and exposed to 15 mg/L CuO-NPs. Exposure to CuSO or CuO-NPs significantly increased superoxide dismutase and catalase activities in fish, as well as serum total protein, glucose, aspartate aminotransferase, alanine aminotransferase, creatinine, and uric acid concentrations. In contrast, most hematological indices in fish significantly decreased after CuSO or CuO-NPs exposure. Moreover, CuSO and CuO-NPs caused a significant increase in the percentage of poikilocytosis and nuclear abnormalities of red blood cells, as well as histopathological changes in the brain, liver, intestine, and kidneys. Importantly, Spirulina supplementation mitigated against physiological disruption caused by CuSO or CuO-NPs.
Topics: Animals; Biomarkers; Cichlids; Copper; Copper Sulfate; Nanoparticles; Oxides; Spirulina
PubMed: 33580857
DOI: 10.1007/s11356-021-12859-0 -
Journal of Clinical Pathology Jul 2023Krüppel-like factor 1 (KLF1) is an erythroid-specific transcription factor playing an important role in erythropoiesis and haemoglobin (Hb) switching. Biallelic KLF1...
AIMS
Krüppel-like factor 1 (KLF1) is an erythroid-specific transcription factor playing an important role in erythropoiesis and haemoglobin (Hb) switching. Biallelic KLF1 mutations can cause haemolytic anaemia with thalassaemia-like syndromes but are rarely reported. We explore the KLF1 mutations in Thai subjects with unexplainable haemolytic anaemia.
METHODS
The study was done on 57 subjects presented with haemolytic anaemia and elevated Hb F without β-thalassaemia diseases. Hb analysis was performed using capillary electrophoresis. Analyses of α-thalassaemia, β-thalassaemia and KLF1 genes were performed using PCR-based methods and DNA sequencing.
RESULTS
Thirteen subjects with compound heterozygous for a known and five new genetic KLF1 interactions were identified, including KLF1:c.519_525dupCGGCGCC/c.892G>C with class 3/2 (n=8), and each subject with new genetic interaction, including KLF1:c.-154C>T;643C>T/c.983G>A with class 3/2, KLF1:c.-154C>T;643C>T/c.809C>G with class 3/2, KLF1:c892G>C/c.983G>A with class 2/2, KLF1:c.892G>C/c.1001C>G with class 2/2 and KLF1:c.1001C>G/c.1003G>A with class 2/2. Most of them had anaemia with Hb levels ranging from 45 to 110 g/L, hypochromic microcytosis, aniso-poikilocytosis, increased Hb F levels (17.9%-47.4%), small amounts of Hb Bart's, regular blood transfusion, hyperbilirubinaemia, increased serum ferritin and nucleated red blood cell.
CONCLUSIONS
Biallelic KLF1 mutations associated with anaemia may not be uncommon in Thailand. Characteristics of haemolytic anaemia, abnormal red cell morphology with nucleated red blood cells and elevated Hb F, and presenting small amounts of Hb Bart's without thalassaemia diseases are useful markers to further investigation of the KLF1 gene.
PubMed: 37507221
DOI: 10.1136/jcp-2023-208945