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The Plant Journal : For Cell and... Sep 2022
Topics: Pollen
PubMed: 36111799
DOI: 10.1111/tpj.15956 -
Scientific Reports Jun 2021Pollen and molds are environmental allergens that are affected by climate change. As pollen and molds exhibit geographical variations, we sought to understand the impact...
Pollen and molds are environmental allergens that are affected by climate change. As pollen and molds exhibit geographical variations, we sought to understand the impact of climate change (temperature, carbon dioxide (CO), precipitation, smoke exposure) on common pollen and molds in the San Francisco Bay Area, one of the largest urban areas in the United States. When using time-series regression models between 2002 and 2019, the annual average number of weeks with pollen concentrations higher than zero increased over time. For tree pollens, the average increase in this duration was 0.47 weeks and 0.51 weeks for mold spores. Associations between mold, pollen and meteorological data (e.g., precipitation, temperature, atmospheric CO, and area covered by wildfire smoke) were analyzed using the autoregressive integrated moving average model. We found that peak concentrations of weed and tree pollens were positively associated with temperature (p < 0.05 at lag 0-1, 0-4, and 0-12 weeks) and precipitation (p < 0.05 at lag 0-4, 0-12, and 0-24 weeks) changes, respectively. We did not find clear associations between pollen concentrations and CO levels or wildfire smoke exposure. This study's findings suggest that spore and pollen activities are related to changes in observed climate change variables.
Topics: Allergens; Climate Change; Confidence Intervals; Fungi; Multivariate Analysis; Pollen; Seasons; Spores, Fungal
PubMed: 34140579
DOI: 10.1038/s41598-021-92178-z -
Acta Scientiarum Polonorum. Technologia... 2020Small amounts of protein can be found in honey, including well known allergen sources, such as plant pollen and honeybee secretions. Despite this, there are few case...
BACKGROUND
Small amounts of protein can be found in honey, including well known allergen sources, such as plant pollen and honeybee secretions. Despite this, there are few case reports describing allergic reactions following the consumption of honey. The aim of this study was to examine the allergenic properties of nectar honey collected throughout the entire beekeeping season from different provinces in Poland.
METHODS
The immunoreactive properties of 20 Polish nectar honeys were analysed using the sera of IgE pollen allergenic patients (n = 5). The botanical origins and pollen of the anemophilous plants in the studied honeys were identified through palynological analysis.
RESULTS
The significant differences in the protein content between the five varieties of honey and the differences in protein pattern and pollen profiles were observed. All of the honey samples contained immunoreactive fractions reacting with IgE present in the sera of patients allergenic to different pollens.
CONCLUSIONS
Although honey allergies are reported relatively rarely, all the tested samples of Polish nectar honeys contained many protein fractions which reacted with the IgE antibodies of allergenic patients. In all samples, the immunoreactive protein band with a molecular weight around 60 kDa, probably secreted by bees, was present. The results do not allow the immunoreactive fractions characteristic for particular honey varieties to be identified.
Topics: Allergens; Honey; Humans; Immunoglobulin E; Plant Nectar; Poland; Pollen
PubMed: 32227694
DOI: 10.17306/J.AFS.0750 -
Journal of the Science of Food and... May 2021Bee pollen is considered as a treasure trove of human and animal nutrients as a result of its extensive nutritional and therapeutic properties. However, the... (Comparative Study)
Comparative Study
BACKGROUND
Bee pollen is considered as a treasure trove of human and animal nutrients as a result of its extensive nutritional and therapeutic properties. However, the sophisticated pollen wall can largely limit the digestibility and bioavailability of these nutrients.
RESULTS
An ultrasonication and high shear technique was used to break the walls of five species of bee pollen, including rape bee pollen, lotus bee pollen, camellia bee pollen, wuweizi bee pollen and apricot bee pollen. We compared the digestibilities of bee pollen with or without wall-disruption. After in vitro and in vivo digestion, unbroken bee pollen grains were still intact and the fragments of wall-disrupted bee pollen still remained as fragments. Mouse in vivo digestion results suggested that the wall-disrupted bee pollen was more easily emptied from the gastrointestinal tract than unbroken bee pollen. After dynamic in vitro digestion, the digestibilities of protein and crude fat in wall-disrupted bee pollen significantly increased to more than 80%; similarly, the release rates of amino acids and reducing sugars in all wall-disrupted samples were almost 1.5 and 2 times as much as those of unbroken samples.
CONCLUSION
Based on the results obtained in the present study, we strongly recommend that bee pollen should be wall-disrupted. © 2020 Society of Chemical Industry.
Topics: Amino Acids; Animals; Bees; Biological Availability; Cell Wall; Digestion; Gastrointestinal Tract; Nutrients; Plant Proteins; Pollen
PubMed: 33124687
DOI: 10.1002/jsfa.10902 -
Cryo Letters 2020Pollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm... (Review)
Review
Pollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to understand the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely in research, plant breeding and germplasm conservation programs.
Topics: Conservation of Natural Resources; Cryopreservation; Plant Breeding; Pollen; Vitrification
PubMed: 33988640
DOI: No ID Found -
The Science of the Total Environment Nov 2022Pollen and spores have been identified as major airborne bio-particles inducing respiratory disorders including allergic rhinitis and atopic dermatitis among...
Pollen and spores have been identified as major airborne bio-particles inducing respiratory disorders including allergic rhinitis and atopic dermatitis among hypersensitive individuals. The present study was conducted with a view to investigating monthly depositional rate of atmospheric palynomorphs to determine the influence of the immediate vegetation on airborne pollen distribution; allergenic activities of dominant atmospheric pollen types at selected study locations in Taraba and Bauchi States, Northeastern Nigeria. Bioaerosols were collected using Tauber-like pollen traps and subjected to standard palynological treatment procedures, microscopy and photomicrography. Plant enumeration within the surrounding vegetation revealed that some airborne pollen types were produced by local plants at the study locations. Spores of Nephrolepis sp., Pteris sp. and a trilete fern, as well as diatoms were also recovered. Crude protein contents of some dominant pollen types; Borreria verticillata (L.) G.F.W. Meyer and Panicum maximum Jacq. for Taraba State; Leucaena leucocephala (Lam.) de Wit. and Terminalia catappa L. for Bauchi State, were quantified and extracted to sensitize Mus musculus mice for serology (ELISA) and haematology (differential and total white blood cell counts). Statistical significance was tested and recorded in the correlation between levels of serological and haematological parameters elicited by each test group; differences between levels of these parameters elicited by each test group and those of the control, as well as at varying sensitization periods. In the Leucaena leucocephala test group, swollen body and histopathological morbid features showing more extensive areas of inflammatory cells and alveoli filled with fluid in the lungs, were recorded in two dead M. musculus, respectively. The study revealed that all the tested pollen types are possible allergens at the study locations, establishing a complexity of interaction among allergy mediators at varied periods of mice sensitization and forming a paradigm of human immune response to the different pollen allergens.
Topics: Allergens; Animals; Humans; Hypersensitivity; Mice; Nigeria; Plants; Pollen; Rhinitis, Allergic, Seasonal; Spores
PubMed: 35780899
DOI: 10.1016/j.scitotenv.2022.157076 -
Methods in Molecular Biology (Clifton,... 2020Effective mutagenesis is critical for connecting traits of interest to specific plant genes. The development of site-directed mutagenesis and sequenced-indexed genetics...
Effective mutagenesis is critical for connecting traits of interest to specific plant genes. The development of site-directed mutagenesis and sequenced-indexed genetics resources in maize allows for targeted analysis of individual genes. These reverse genetics approaches have the potential for confirmation bias by only studying candidate genes for association with traits of interest. Genetic screens of induced, random mutations are important for identifying novel loci as well as interacting factors for known mutant loci. Chemical mutagenesis provides very high mutation rates and can be used for a variety of screen designs. This chapter provides an updated protocol for ethyl methanesulfonate (EMS) mutagenesis of maize pollen using paraffin or mineral oil. Mutagenesis occurs in mature pollen causing nonconcordant endosperm and embryo genotypes as well as sectored M1 plants. Considerations for these factors in genetic screens are discussed.
Topics: Endosperm; Ethyl Methanesulfonate; Genes, Plant; Mutagenesis; Mutagens; Mutation; Mutation Rate; Pollen; Zea mays
PubMed: 31975293
DOI: 10.1007/978-1-0716-0342-0_3 -
The Science of the Total Environment Aug 2022Particulate matter (PM) and pollen interaction, either airborne or at the respiratory mucosa needs further clarification, as allergic reaction intensification can be...
Particulate matter (PM) and pollen interaction, either airborne or at the respiratory mucosa needs further clarification, as allergic reaction intensification can be related to the PM physical characteristics and toxicity. This study aimed to investigate the physical-chemical properties of PM that can adhere to the pollen wall during its transport or inhalation, using Quercus spp. as a model, in three Portuguese cities with different geographical locations, meteorological influence and urbanization levels. Possible sources were evaluated through air masses trajectory analysis using the HYSPLIT model and correlation with meteorological factors. The sampling was performed using a 7-days Hirst-type volumetric sampler, and the pollen grains were observed using a Field Emission Electron Probe Microanalyser for PM analysis. A secondary electron image of each pollen grain was taken, to determine the adhered particles characteristics and energy dispersive x-ray spectroscopy (EDS) spectra were obtained for individual particles. A total of 484 pollen grains was observed, with 7683 particles counted and 1914 EDS spectra analyzed. The particle's equivalent diameter ranged from 0.3-16 μm, with most having a diameter < 3 μm. For the three cities, there were significant differences in the number of particles per pollen and the % area occupied by the particles. Particles adhered were mainly Si-rich, but variations in other dominant groups were observed. For Évora and Guarda, Ca-rich, SO-rich were second and third more representative, while Porto were Organic and Cl-rich. Metals&Oxides were found in all cities with the highest number in Porto. P-rich particles were only found in Évora. Sea salt particles were observed in Évora, coincide with air mass trajectories possible carrying them from the Mediterranean Sea. In conclusion, the PM physical characteristics are similar between the studied cities, however, the dominant chemical composition is different, certainly impacting the exposome influence and pollen-allergy intensification towards the same pollen type and concentration.
Topics: Air Pollutants; Cities; Environmental Monitoring; Particle Size; Particulate Matter; Pollen; Quercus
PubMed: 35378190
DOI: 10.1016/j.scitotenv.2022.154892 -
International Journal of Molecular... Feb 2022Although pollen structure and morphology evolved toward the optimization of stability and fertilization efficiency, its performance is affected by harsh environmental... (Review)
Review
Although pollen structure and morphology evolved toward the optimization of stability and fertilization efficiency, its performance is affected by harsh environmental conditions, e.g., heat, cold, drought, pollutants, and other stressors. These phenomena are expected to increase in the coming years in relation to predicted environmental scenarios, contributing to a rapid increase in the interest of the scientific community in understanding the molecular and physiological responses implemented by male gametophyte to accomplish reproduction. Here, after a brief introduction summarizing the main events underlying pollen physiology with a focus on polyamine involvement in its development and germination, we review the main effects that environmental stresses can cause on pollen. We report the most relevant evidence in the literature underlying morphological, cytoskeletal, metabolic and signaling alterations involved in stress perception and response, focusing on the final stage of pollen life, i.e., from when it hydrates, to pollen tube growth and sperm cell transport, with these being the most sensitive to environmental changes. Finally, we hypothesize the molecular mechanisms through which polyamines, well-known molecules involved in plant development, stress response and adaptation, can exert a protective action against environmental stresses in pollen by decoding the essential steps and the intersection between polyamines and pollen tube growth mechanisms.
Topics: Fertility; Germination; Pollen Tube; Polyamines; Signal Transduction; Stress, Physiological
PubMed: 35163795
DOI: 10.3390/ijms23031874 -
Food Research International (Ottawa,... Feb 2024Bee pollen is hailed as a treasure trove of human nutrition and has progressively emerged as the source of functional food and medicine. This review conducts a... (Review)
Review
Bee pollen is hailed as a treasure trove of human nutrition and has progressively emerged as the source of functional food and medicine. This review conducts a compilation of nutrients and phytochemicals in bee pollen, with particular emphasis on some ubiquitous and unique phenolamides and flavonoid glycosides. Additionally, it provides a concise overview of the diverse health benefits and therapeutic properties of bee pollen, particularly anti-prostatitis and anti-tyrosinase effects. Furthermore, based on the distinctive structural characteristics of pollen walls, a substantial debate has persisted in the past concerning the necessity of wall-disruption. This review provides a comprehensive survey on the necessity of wall-disruption, the impact of wall-disruption on the release and digestion of nutrients, and wall-disruption techniques in industrial production. Wall-disruption appears effective in releasing and digesting nutrients and exploiting bee pollen's bioactivities. Finally, the review underscores the need for future studies to elucidate the mechanisms of beneficial effects. This paper will likely help us gain better insight into bee pollen to develop further functional foods, personalized nutraceuticals, cosmetics products, and medicine.
Topics: Bees; Humans; Animals; Pollen; Nutrients; Flavonoids; Glycosides; Phytochemicals
PubMed: 38309905
DOI: 10.1016/j.foodres.2024.113934