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Environmental Science and Pollution... Mar 2023Health hazards associated with microplastics (MPs) remain largely unknown, and the effects of aged MPs, one of their persistent forms, are poorly characterized. Male ICR...
Health hazards associated with microplastics (MPs) remain largely unknown, and the effects of aged MPs, one of their persistent forms, are poorly characterized. Male ICR mice were intratracheally instilled with 0.01 and 1 mg/day pristine and ultraviolet (UV)-aged polystyrene microplastics (PS and APS) with an average diameter of 4 - 5 μm daily for 1 week. UV irradiation caused the PS to have a rough surface, become fragmented, and increase their carbonyl groups. Both PS and APS caused structural damage to the mouse gut, liver, spleen, and testis. Inflammatory infiltration in liver, swollen and congested gut, and loose spleen globules, as well as the loose interstitium of the seminiferous tubules in testis were found in 1 mg/day APS group. Increases in serum alanine aminotransferase and immunoglobulin A levels in 1 mg/day APS group (p < 0.05) demonstrated that APS exposure could induce greater liver and spleen functional damage than PS. Meanwhile, triglyceride and total cholesterol levels in liver were enhanced in 1 mg/day APS group (p < 0.05). Superoxide dismutase and glutathione contents in 0.01 and 1 mg/day APS groups significantly decreased (p < 0.05), which suggesting that PS and APS could interfere with the antioxidant capacity in mice. Nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) levels in the PS and APS groups showed significant increases in the liver and testis (p < 0.05), and a significant decrease in the spleen (p < 0.05), which were analyzed to get a first survey for Nrf2/HO-1-mediated tissue-specific defense mechanisms. In conclusion, acute exposure to PS and APS induced potential metabolic disorders, and APS could produce more serious immune damage and reproductive toxicity. These findings provide new insights in health risk assessment of aged MPs.
Topics: Mice; Male; Animals; Polystyrenes; Microplastics; Plastics; NF-E2-Related Factor 2; Organ Specificity; Mice, Inbred ICR; Defense Mechanisms
PubMed: 36602732
DOI: 10.1007/s11356-022-24918-1 -
Chemico-biological Interactions Nov 2022Human gastrointestinal cells can be exposed to different xenobiotics present in food or drinking water. In this work, we assessed the cytotoxicity of polystyrene...
Human gastrointestinal cells can be exposed to different xenobiotics present in food or drinking water. In this work, we assessed the cytotoxicity of polystyrene nanoparticles (PS-NPs) and how it is impacted by fluoride (F) presence. We decided to examine PS-NPs and F which can be easily found in drinking water and food. Commercially available amine-modified 100 nm PS-NPs were used in the study. Scanning Electron Microscopy with Electron Dispersive Spectroscopy (SEM-EDS) and Dynamic Light Scattering (DLS) were used to characterize PS-NPs. The colon cell lines (HT-29, Caco-2, CCD 841 CoN) were used. Cytotoxicity of PS-NPs and F alone or in co-exposition were assessed with MTT assay in a time- and concentration-dependent manner. Flow cytometry was used to measure reactive oxygen species (ROS) production, cell cycle distribution, and apoptosis analysis. Transmission electron microscopy (TEM) was used to determine whether PS-NPs and/or F can cause ultrastructure changes in the cells. We have shown that PS-NPs are cytotoxic to human colon cells in a time- and concentration-dependent manner. PS-NPs did not impact neither intracellular ROS production nor the cells cell cycle distribution. However, if HT-29 cells were co-exposed to PS-NPs and F, an increased number of cells in G0/G1 phase and decreased number of cells in G2/M were observed. PS-NPs can cause apoptosis in HT-29 cells, this effect was enhanced if cells were co-exposed to PS-NP and F. PS-NPs were internalised by the cells and caused ultrastructure changes. Fluoride itself (1 mM) was not cytotoxic to the cells and did not cause any changes in the ultrastructure of the cells. We have proven that polystyrene nanoparticles can be cytotoxic to human gastrointestinal cells and this effect is enhanced by fluoride.
Topics: Amines; Caco-2 Cells; Drinking Water; Fluorides; Humans; Nanoparticles; Polystyrenes; Reactive Oxygen Species
PubMed: 36165825
DOI: 10.1016/j.cbi.2022.110169 -
Environmental Pollution (Barking, Essex... Feb 2024The extensive utilization and inadequate handling of plastics have resulted in severe environmental ramifications. In particular, plastics composed solely of a... (Review)
Review
The extensive utilization and inadequate handling of plastics have resulted in severe environmental ramifications. In particular, plastics composed solely of a carbon-carbon (C-C) backbone exhibit limited degradation due to the absence of hydrolyzable functional groups. Plastics with enduring longevity in the natural environment are susceptible to environmental factors and their intrinsic properties, subsequently undergoing a series of aging processes that culminate in biodegradation. This article focuses on polystyrene (PS), which constitutes 20% of total plastic waste, as a case study. Initially, the application of PS in life and the impacts it poses are introduced. Following that, the key factors influencing the aging of PS are discussed, primarily encompassing its properties (e.g., surface characteristics, additives) and environmental factors (e.g., water matrices, biofilms). Lastly, an overview of microbial degradation of PS is provided, including potential microorganisms involved in PS degradation (bacteria, fungi, algae, and insects), four processes of microbial degradation (colonization, bio-fragmentation, assimilation, and mineralization), and potential mechanisms of microbial degradation. This study provides a comprehensive understanding of the multifaceted influences affecting the aging and biodegradation mechanisms of PS, thereby contributing valuable insights for the future management of plastic pollution.
Topics: Polystyrenes; Plastics; Biodegradation, Environmental; Carbon
PubMed: 38016589
DOI: 10.1016/j.envpol.2023.123034 -
Journal of Hazardous Materials Jun 2024Micro- and nanoplastics (MNPs) are ubiquitous in the environment, resulting in the uptake of MNPs by a variety of organisms, including humans, leading to particle-cell...
Micro- and nanoplastics (MNPs) are ubiquitous in the environment, resulting in the uptake of MNPs by a variety of organisms, including humans, leading to particle-cell interaction. Human macrophages derived from THP-1 cell lines take up Polystyrene (PS), a widespread plastic. The question therefore arises whether primary human macrophages also take up PS micro- and nanobeads (MNBs) and how they react to this stimulation. Major aim of this study is to visualize this uptake and to validate the isolation of macrophages from peripheral blood mononuclear cells (PBMCs) to assess the impact of MNPs on human macrophages. Uptake of macrophages from THP-1 cell lines and PBMCs was examined by transmission electron microscopy (TEM), scanning electron microscopy and live cell imaging. In addition, the reaction of the macrophages was analyzed in terms of metabolic activity, cytotoxicity, production of reactive oxygen species (ROS) and macrophage polarization. This study is the first to visualize PS MNBs in primary human cells using TEM and live cell imaging. Metabolic activity was size- and concentration-dependent, necrosis and ROS were increased. The methods demonstrated in this study outline an approach to assess the influence of MNP exposure on human macrophages and help investigating the consequences of worldwide plastic pollution.
Topics: Humans; Macrophages; Reactive Oxygen Species; Polystyrenes; THP-1 Cells; Microplastics; Leukocytes, Mononuclear; Nanoparticles; Cell Survival; Microscopy, Electron, Transmission; Particle Size
PubMed: 38642497
DOI: 10.1016/j.jhazmat.2024.134253 -
NanoImpact Apr 2023The uptake of microplastic particles (MPP) by organisms is frequently described and poses a potential risk for these organisms and ultimately for humans either through...
The uptake of microplastic particles (MPP) by organisms is frequently described and poses a potential risk for these organisms and ultimately for humans either through direct uptake or trophic transfer. Currently, the in-situ detection of MPP in organisms is typically based on histological examination of tissue sections after uptake of fluorescently-labelled MPP and is thus not feasible for environmental samples. The alternative approach is purification of MPP from whole organisms or organs by chemical digestion and subsequent spectroscopic detection (FT-IR or Raman). While this approach is feasible for un-labelled particles it goes along with loss of any spatial information related to the location in the tissue. In our study we aimed at providing a workflow for the localisation and identification of non-fluorescent and fluorescent polystyrene (PS) particles (fragments, size range 2-130 μm) in tissue sections of the model organism Eisenia fetida with Raman spectroscopic imaging (RSI). We provide methodological approaches for the preparation of the samples, technical parameters for the RSI measurements and data analysis for PS differentiation in tissue sections. The developed approaches were combined in a workflow for the in-situ analysis of MPP in tissue sections. The spectroscopic analysis requires differentiation of spectra of MPP and interfering compounds, which is challenging given the complexity of tissue. Therefore, a classification algorithm was developed to differentiate PS particles from haem, intestinal contents and surrounding tissue. It allows the differentiation of PS particles from protein in the tissue of E. fetida with an accuracy of 95%. The smallest PS particle detected in the tissue was 2 μm in diameter. We show that it is possible to localise and identify non-fluorescent and fluorescent ingested PS particles directly in tissue sections of E. fetida in the gut lumen and the adjacent tissue.
Topics: Humans; Polystyrenes; Plastics; Spectroscopy, Fourier Transform Infrared; Microplastics; Spectrum Analysis, Raman
PubMed: 37119946
DOI: 10.1016/j.impact.2023.100465 -
World Journal of Microbiology &... Mar 2024Polystyrene (PS) is frequently used in the plastics industry. However, its structural stability and difficulty to break down lead to an abundance of plastic waste in the... (Review)
Review
Polystyrene (PS) is frequently used in the plastics industry. However, its structural stability and difficulty to break down lead to an abundance of plastic waste in the environment, resulting in micro-nano plastics (MNPs). As MNPs are severe hazards to both human and environmental health, it is crucial to develop innovative treatment technologies to degrade plastic waste. The biodegradation of plastics by insect gut microorganisms has gained attention as it is environmentally friendly, efficient, and safe. However, our knowledge of the biodegradation of PS is still limited. This review summarizes recent research advances on PS biodegradation by gut microorganisms/enzymes from insect larvae of different species, and schematic pathways of the degradation process are discussed in depth. Additionally, the prospect of using modern biotechnology, such as genetic engineering and systems biology, to identify novel PS-degrading microbes/functional genes/enzymes and to realize new strategies for PS biodegradation is highlighted. Challenges and limitations faced by the application of genetically engineered microorganisms (GEMs) and multiomics technologies in the field of plastic pollution bioremediation are also discussed. This review encourages the further exploration of the biodegradation of PS by insect gut microbes/enzymes, offering a cutting-edge perspective to identify PS biodegradation pathways and create effective biodegradation strategies.
Topics: Animals; Humans; Polystyrenes; Gastrointestinal Microbiome; Plastics; Biodegradation, Environmental; Insecta
PubMed: 38530548
DOI: 10.1007/s11274-024-03932-0 -
Environmental Pollution (Barking, Essex... Feb 2024Microplastics pollution has garnered significant attention in recent years. The unique cross-linked structure of polystyrene microplastics makes them difficult to...
Microplastics pollution has garnered significant attention in recent years. The unique cross-linked structure of polystyrene microplastics makes them difficult to biodegrade. In this study, we investigated the microbial community in landfill soil that has the ability to degrade polystyrene, as well as two isolated strains, named Lysinibacillus sp. PS-L and Pseudomonas sp. PS-P. The maximum weight loss of polystyrene film and microplastic in 30 days is 2.25% and 6.99% respectively. The water contact angle of polystyrene film decreased by a maximum of 35.70% during biodegradation. The increase in hydrophilicity is attributed to the oxidation reaction and formation of hydroxyl groups during the degradation of polystyrene. The carbon and oxygen element contents of polystyrene decreased and increased by a maximum of 3.81% and 0.79% respectively. The peak intensity changes at wavelengths of 3285-3648 cm and 1652 cm in Fourier transform infrared spectroscopy confirmed the formation of hydroxyl and carbonyl groups. Furthermore, quantitative PCR revealed the gene expression levels of alkane monooxygenase and alcohol dehydrogenase were upregulated by 8.8-fold and 8.5-fold respectively in PS biodegradation. Additionally, genome annotation of Pseudomonas sp. PS-P identified nine genes associated with polystyrene metabolism. These findings highlight Pseudomonas sp. PS-P as a potential candidate strain for polystyrene degradation enzymes or genes. Thus, they lay the groundwork for understanding the potential metabolic mechanisms and pathways involved in polystyrene degradation.
Topics: Polystyrenes; Plastics; Microplastics; Bacteria; Biodegradation, Environmental; Pseudomonas
PubMed: 38128711
DOI: 10.1016/j.envpol.2023.123202 -
Chemosphere Jul 2022The combined effect of microplastics and pharmaceuticals on aquatic organisms is an issue of concern. In this laboratory study, we evaluated the combined effect of...
The combined effect of microplastics and pharmaceuticals on aquatic organisms is an issue of concern. In this laboratory study, we evaluated the combined effect of polystyrene microplastics (2-μm diameter) and diazepam on the social behavior of medaka (Oryzias latipes) by using the shoaling behavior test with five treatment groups: solvent control, polystyrene microplastics exposure (0.04 mg/L), low-concentration diazepam exposure (0.03 mg/L), high-concentration diazepam exposure (0.3 mg/L), and polystyrene microplastics and low-concentration diazepam co-exposure. After 7 days of exposure, the shoal-leaving behavior of the high-concentration diazepam exposure group (8.9 ± 8.3 counts/medaka) and the co-exposure group (6.8 ± 6.7 counts/medaka) was significantly greater than that in the solvent control group (1.8 ± 2.6 counts/medaka). Even after 5 days of recovery, medaka in the co-exposure group left the shoal more often (7.3 ± 5.0 counts/medaka) than those in the solvent control group (2.6 ± 2.6 counts/medaka), whereas the shoal-leaving behavior in other exposure groups, except for the high-concentration diazepam exposure group, was restored. Our findings show that the combined effects of diazepam and polystyrene microplastics suppressed medaka social behavior, suggesting that the presence of microplastics can enhance the adverse effects of pollutants on the social behavior of aquatic organisms.
Topics: Animals; Diazepam; Microplastics; Oryzias; Plastics; Polystyrenes; Social Behavior; Solvents; Water Pollutants, Chemical
PubMed: 35341767
DOI: 10.1016/j.chemosphere.2022.134403 -
Particle and Fibre Toxicology Dec 2023Nanoplastics (NPs) are omnipresent in our lives as a new type of pollution with a tiny size. It can enter organisms from the environment, accumulate in the body, and be...
BACKGROUND
Nanoplastics (NPs) are omnipresent in our lives as a new type of pollution with a tiny size. It can enter organisms from the environment, accumulate in the body, and be passed down the food chain. Inflammatory bowel disease (IBD) is a nonspecific intestinal inflammatory disease that is recurrent and prevalent in the population. Given that the intestinal features of colitis may affect the behavior and toxicity of NPs, it is imperative to clarify the risk and toxicity mechanisms of NPs in colitis models.
METHODS AND RESULTS
In this study, mice were subjected to three cycles of 5-day dextran sulfate sodium (DSS) exposures, with a break of 7 to 11 days between each cycle. After the first cycle of DSS exposure, the mice were fed gavagely with water containing 100 nm polystyrene nanobeads (PS-NPs, at concentrations of 1 mg/kg·BW, 5 mg/kg·BW and 25 mg/kg·BW, respectively) for 28 consecutive days. The results demonstrated that cyclic administration of DSS induced chronic inflammation in mice, while the standard drug "5-aminosalicylic acid (5-ASA)" treatment partially improved colitis manifestations. PS-NPs exacerbated intestinal inflammation in mice with chronic colitis by activating the MAPK signaling pathway. Furthermore, PS-NPs aggravated inflammation, oxidative stress, as well as hepatic lipid metabolism disturbance in the liver of mice with chronic colitis.
CONCLUSION
PS-NPs exacerbate intestinal inflammation and injury in mice with chronic colitis. This finding highlights chronically ill populations' susceptibility to environmental hazards, which urgent more research and risk assessment studies.
Topics: Mice; Animals; Polystyrenes; Lipid Metabolism; Colitis; Inflammation; Oxidative Stress; Liver; Chronic Disease; Mice, Inbred C57BL; Disease Models, Animal
PubMed: 38110964
DOI: 10.1186/s12989-023-00560-8 -
The Science of the Total Environment Nov 2023Understanding nanoplastic (NP, or nanoparticle in general) toxicity requires establishing the causal relationships between the physical properties of the nanoparticles...
Understanding nanoplastic (NP, or nanoparticle in general) toxicity requires establishing the causal relationships between the physical properties of the nanoparticles and their biological impact. We use spectroscopic, zeta-potential, and dynamic light scattering (DLS) techniques to investigate the formation, structure, and catalytic properties of hemoglobin corona complexes with polystyrene NPs (0-10 mg/mL) of various diameters (20, 50, 100, 500, and 5000 nm). Resonance light scattering, zeta-potential analysis, and DLS demonstrated that hemoglobin corona complexes formed different forms of aggregates with NPs in terms of diameter. Medium-sized (100 nm) NPs induced the most significant conformational alterations in the protein corona compared to smaller and larger ones, which was revealed by spectroscopic assays. However, the catalase-like activity of hemoglobin was promoted in the presence of 100 nm NPs by as high as 35.2 %. NP curvature and surface area are antagonistic factors that govern the conformation of proteins together. This also suggests that 100 nm NPs are more likely to disrupt protein-dependent physiological processes at a given mass concentration than small or large NPs.
Topics: Polystyrenes; Microplastics; Hemoglobins; Nanoparticles; Dynamic Light Scattering
PubMed: 37478940
DOI: 10.1016/j.scitotenv.2023.165617