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Endocrinology Jan 2021The role of depolarization in the inverse glucose-dependence of glucagon secretion was investigated by comparing the effects of KATP channel block and of high potassium....
The role of depolarization in the inverse glucose-dependence of glucagon secretion was investigated by comparing the effects of KATP channel block and of high potassium. The secretion of glucagon and insulin by perifused mouse islets was simultaneously measured. Lowering glucose raised glucagon secretion before it decreased insulin secretion, suggesting an alpha cell-intrinsic signal recognition. Raising glucose affected glucagon and insulin secretion at the same time. However, depolarization by tolbutamide, gliclazide, or 15 mM KCl increased insulin secretion before the glucagon secretion receded. In contrast to the robust depolarizing effect of arginine and KCl (15 and 40 mM) on single alpha cells, tolbutamide was of variable efficacy. Only when applied before other depolarizing agents had tolbutamide a consistent depolarizing effect and regularly increased the cytosolic Ca2+ concentration. When tested on inside-out patches tolbutamide was as effective on alpha cells as on beta cells. In the presence of 1 µM clonidine, to separate insulinotropic from glucagonotropic effects, both 500 µM tolbutamide and 30 µM gliclazide increased glucagon secretion significantly, but transiently. The additional presence of 15 or 40 mM KCl in contrast led to a marked and lasting increase of the glucagon secretion. The glucagon secretion by SUR1 knockout islets was not increased by tolbutamide, whereas 40 mM KCl was of unchanged efficiency. In conclusion a strong and sustained depolarization is compatible with a marked and lasting glucagon secretion. KATP channel closure in alpha cells is less readily achieved than in beta cells, which may explain the moderate and transient glucagonotropic effect.
Topics: Animals; Arginine; Calcium; Cell Membrane; Female; Gliclazide; Glucagon; Glucagon-Secreting Cells; Glucose; Hypoglycemic Agents; Insulin; Insulin Secretion; Insulin-Secreting Cells; KATP Channels; Membrane Potentials; Mice; Potassium; Potassium Chloride; Tolbutamide
PubMed: 32790843
DOI: 10.1210/endocr/bqaa136 -
Molecules (Basel, Switzerland) Sep 2023Sodium chloride (NaCl) is a commonly used additive in minimally processed fish-based products. The addition of NaCl to fish products and packaging in a modified...
Sodium chloride (NaCl) is a commonly used additive in minimally processed fish-based products. The addition of NaCl to fish products and packaging in a modified atmosphere is usually efficient with regard to limiting the occurrence of the aquatic environmental pathogen Given the negative effects of excess NaCl in the diet, there is a growing demand to reduce NaCl in food products with safer substituents, but the knowledge of their impact on antibiotic resistant is limited. This study aimed to evaluate the physiological and transcriptome characteristics of NT06 isolated from fish and to determine the effect of selected concentrations of alternative NaCl compounds (KCl/NaL/NaC) on the NT06 virulence phenotype and genotype. In the study, among the isolated microorganisms, NT06 showed the highest antibiotic resistance (to ampicillin, ceftriaxone, nalidixic acid, and norfloxacin) and the ability to grow at 4 °C. The Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) revealed the presence of 24 and 134 gene products assigned to AMR and VF in the NT06 transcriptome, respectively. KCl, KCl/NaL and KCl/NaL/NaC inhibited pyocyanin biosynthesis, elastase activity, and protease activity from 40 to 77%. The above virulence phenotypic observations were confirmed via RT-qPCR analyses, which showed that all tested AMR and VF genes were the most downregulated due to KCl/NaL/NaC treatment. In conclusion, this study provides insight into the potential AMR and VF among foodborne and the possible impairment of those features by KCl, NaL, and NaC, which exert synergistic effects and can be used in minimally processed fish-based products.
Topics: Animals; Virulence; Anti-Bacterial Agents; Pseudomonas aeruginosa; Sodium Citrate; Sodium Lactate; Potassium Chloride; Sodium Chloride; Drug Resistance, Bacterial; Virulence Factors; Pseudomonas Infections
PubMed: 37764430
DOI: 10.3390/molecules28186654 -
International Journal of Gastronomy and... Mar 2022High sodium intakes represent an important risk factor for hypertension, cardiovascular diseases and kidney diseases. Even during the current COVID-19 pandemic,...
High sodium intakes represent an important risk factor for hypertension, cardiovascular diseases and kidney diseases. Even during the current COVID-19 pandemic, hypertension was related to higher mortality rate in patients with coronavirus. Thus, it is necessary to apply strategies to reduce or replace sodium content in food most widely consumed, like bread. This work aimed at determining the sensorial potassium threshold when potassium chloride is used as a sodium chloride replacer in bread formulation, and at analyzing the effects of such replacement on the properties of dough and on the technological and sensorial quality of bread. A decrease was observed in dough rheological properties with NaCl reduction in the formulation. Sensorial potassium threshold was determined and KCl was used in bread formulation as a NaCl replacement up to 0.92% of the regular salt content (2%) undetected by its characteristic taste. NaCl reduction resulted in bread with lower specific volume, higher firmness, faster staling and clearer crust. KCl bread showed similar technological to 2% NaCl bread. Finally, it was possible to replace 50% of NaCl with KCl without reducing quality and consumer acceptability.
PubMed: 36568859
DOI: 10.1016/j.ijgfs.2022.100486 -
The Journal of Physical Chemistry. B Dec 2019Neutron diffraction with isotopic substitution (NDIS) experiments were done on both natural abundance potassium and isotopically labeled KCl heavy water solutions to...
Neutron diffraction with isotopic substitution (NDIS) experiments were done on both natural abundance potassium and isotopically labeled KCl heavy water solutions to characterize the solvent structuring around the potassium ion in water. Preliminary measurements suggested that the literature value for the coherent neutron scattering length (2.69 fm) for K was significantly in error. This value was remeasured using a neutron powder diffractometer and found to be 2.40 fm. This revision increases significantly the contrast between the natural abundance K and K by about 30% (from 1.0 to 1.3 fm). The experimentally determined structure factor of the potassium ion was then compared to that calculated from molecular dynamics (MD) simulations. Previous neutron scattering measurements of potassium gave a solvation number of 5.5 (see below). In this study, the NDIS and MD results are in good agreement and allowed us to derive a coordination number of 6.1 for water molecules and 0.8 for chloride ions around each K ion in 4 molal aqueous KCl solution.
Topics: Molecular Conformation; Molecular Dynamics Simulation; Neutron Diffraction; Potassium Chloride; Solutions; Water
PubMed: 31769976
DOI: 10.1021/acs.jpcb.9b08422 -
PloS One 2023To investigate the effect of the addition of a low concentration of sodium chloride (NaCl) and potassium chloride (KCl) solutions on the tear ferning (TF) patterns of...
PURPOSE
To investigate the effect of the addition of a low concentration of sodium chloride (NaCl) and potassium chloride (KCl) solutions on the tear ferning (TF) patterns of tears collected from humans.
METHODS
A tear sample (20 μL) was collected from the right eye of 23 males and 7 females (25.4 ± 6.6 years). The tears were collected in one sitting for healthy subjects (N = 13). For dry eye participants (N = 17), the tear samples were collected in two separate settings with five minutes gap in between. A sample (1 μL) from each tear was dried on a glass slide, and the obtained ferns were observed using a microscope and graded using the five-point TF grading scale. Mixtures of tear samples (0.5 μL) and different volumes (0.5-2.5 μL) of each electrolyte (10-30 mg in 100 mL of water) solution were prepared, and their TF patterns were recorded and compared with those of the corresponding pure tears.
RESULTS
Significant improvements (Wilcoxon test, P < 0.001) have been seen in the TF grades of the tear samples after the addition of NaCl and KCl solutions. A significant difference (Wilcoxon test, P = 0.016) was found between the TF grades when NaCl and KCl solutions were added to the tear samples. The TF grades of pure tears collected from dry-eye subjects ranged from 2.1 to 3.5, based on the five points grading scale, and decreased to be in the range of 0.4 to 1.6 after the addition of electrolyte solutions. While the TF grades of pure tears collected from normal-eye ranged from 1.2 to 1.9 and improved after the addition of electrolyte solution to be in the range of 0.4 to 1.5.
CONCLUSIONS
The TF test was used in vitro to assess the impact of the addition of a low concentration of sodium and potassium chloride solutions on tears collected from humans. The TF grades of human tears significantly improved after the addition of either sodium or potassium chloride solution. The mechanism for the improvement in TF grades due to the addition of electrolyte solutions must be investigated.
Topics: Male; Female; Humans; Sodium Chloride; Potassium Chloride; Dry Eye Syndromes; Lacerations; Electrolytes; Tears; Sodium
PubMed: 36735697
DOI: 10.1371/journal.pone.0280853 -
Journal of the American College of... Aug 2020
Topics: Aged; Diuretics; Furosemide; Heart Failure; Humans; Prescriptions; Sodium Potassium Chloride Symporter Inhibitors
PubMed: 32762902
DOI: 10.1016/j.jacc.2020.06.044 -
American Journal of Hypertension Jul 2022
Topics: Adrenergic beta-Antagonists; Antihypertensive Agents; Diuretics; Edema; Humans; Hypertension; Prescriptions; Sodium Potassium Chloride Symporter Inhibitors
PubMed: 35302582
DOI: 10.1093/ajh/hpac030 -
The American Journal of Clinical... Jul 2021Sodium intake in the USA exceeds recommendations. The replacement of added sodium chloride (NaCl) with potassium chloride (KCl) provides a potential strategy to reduce...
BACKGROUND
Sodium intake in the USA exceeds recommendations. The replacement of added sodium chloride (NaCl) with potassium chloride (KCl) provides a potential strategy to reduce sodium intake.
OBJECTIVE
The purpose of this study was to quantitatively estimate changes in intakes of sodium and potassium by the US population assuming use of potassium-based NaCl replacers in top dietary sodium sources.
METHODS
Data collected in the What We Eat in America (WWEIA) component of the 2015-2016 and 2009-2010 NHANES were used to identify top-ranking sources of dietary sodium among the population aged 2 y and older based on contributions from food categories aligning with the FDA draft guidance for voluntary sodium reduction. Predicted nutrient intakes were estimated in models assuming total and feasible and practical (F&P) replacement of added NaCl with KCl in foods and ingredients within the top food sources of sodium. An expert elicitation was conducted to collect information on the F&P KCl replacement of added NaCl.
RESULTS
Using 2015-2016 consumption data, the total replacement of added NaCl with KCl in the 18 top-ranking sources of dietary sodium results in a predicted sodium intake of 2004 mg/d from the replacement of 1406 mg/d sodium with 1870 mg/d potassium as KCl. Modeled F&P replacement predicted sodium intakes of 3117 mg/d (range of 2953 to 3255 mg/d) from the replacement of 294 mg/d sodium (155 to 457 mg/d) with 390 mg/d potassium (206 to 608 mg/d). Similar results are seen with 2009-2010 data.
CONCLUSIONS
The F&P replacement of NaCl with KCl in top-ranking sources of dietary sodium modeled in this study can result in decreased sodium to a level consistent with the short-term intake goal targeted by the FDA of 3000 mg/d, with the mean potassium intake remaining in the range recommended for the apparently healthy population.
Topics: Cross-Sectional Studies; Food; Humans; Models, Biological; Nutrition Surveys; Potassium Chloride; Potassium, Dietary; Sodium, Dietary; United States
PubMed: 33755042
DOI: 10.1093/ajcn/nqab020 -
Carbohydrate Polymers Sep 2022With the increasing interests in the fields of wearable devices, it is essential yet also challenging to develop electronic skin with customized functionalities,...
With the increasing interests in the fields of wearable devices, it is essential yet also challenging to develop electronic skin with customized functionalities, especially for harsh conditions. Herein, by using KCl as both anti-solvent for cellulose regeneration and ionic charge carrier in the cellulose gel network, cellulose/KCl/sorbitol organohydrogel (CKS) combining transparency (over 95% at 550 nm), stretchability (235%), high conductivity (3.88 S/m), and low temperature tolerance (-51.8 °C) was prepared. The CKS based electronic skin achieved simultaneous monitoring of object contact-separation/pressure, stretching/bending and thermal variation, with excellent reliability and stability even in harsh conditions, resembling the human skin with multiply functions. The CKS based electronic skin as efficient human-machine interface was also demonstrated. Furthermore, the CKS based triboelectric nanogenerator delivered a power density of 991 mW/m, potential as mechanical energy harvesters for wearable devices. We believe the present work will inspire the development of cellulose based skin-like materials and contribute to the comprehensive utilization of naturel polymer in the field of smart devices.
Topics: Cellulose; Electric Conductivity; Humans; Hydrogels; Potassium Chloride; Reproducibility of Results; Sorbitol; Wearable Electronic Devices
PubMed: 35725202
DOI: 10.1016/j.carbpol.2022.119645 -
European Heart Journal Aug 2023
Topics: Humans; Diuretics; Sodium Potassium Chloride Symporter Inhibitors; Metolazone; Heart Failure
PubMed: 37572039
DOI: 10.1093/eurheartj/ehad463