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International Journal of Systematic and... Aug 2020A strain of an obligately anaerobic, Gram-stain-negative rod-shaped bacterium is described by phenotypical, biochemical and genotypical characterization. This strain...
A strain of an obligately anaerobic, Gram-stain-negative rod-shaped bacterium is described by phenotypical, biochemical and genotypical characterization. This strain A2879 was isolated from an abscess swab of a patient sampled during routine care at hospital. Phylogenetic analyses (full-length 16S rRNA gene and whole-genome sequence) revealed the strain to belong to the genus , but to be distant from recognized species, with the closest relationship to . Unambiguous identification also proved possible by MALDI-TOF MS. The genomic DNA G+C content was 41.5 mol%. Strain A2879 was moderately saccharolytic and proteolytic. The most abundant cellular long-chain fatty acids were anteiso-C and iso-C. In view of these data, strain A2879 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is A2879 (=DSM 108027=CCOS 1233=CCUG72808). As this strain has been isolated from a clinical sample, it is considered relevant for human medicine and health in general, and in particular for the fields of clinical microbiology and infectious diseases. This description will enable routine and research laboratories alike to easily identify the novel taxon, allowing its role in the context of human health and disease or microbiota to be further elucidated.
Topics: Abscess; Adult; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Germany; Humans; Male; Phylogeny; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 32735207
DOI: 10.1099/ijsem.0.004316