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Skin Health and Disease Sep 2021We present a case of a 54-year-old male with multiple myeloma (MM) who presented with widespread pruritic erythematous lesions following ixazomib treatment. This...
We present a case of a 54-year-old male with multiple myeloma (MM) who presented with widespread pruritic erythematous lesions following ixazomib treatment. This occurred after his third cycle of treatment with ixazomib, thalidomide and dexamethasone and was controlled by potent steroids and temporary cessation of ixazomib. The strong correlation between the timeline of the rash, ixazomib treatment and subsequent cessation led to a diagnosis of a drug-induced rash. Skin biopsy histology, immunochemistry and the absence of monoclonal T-cell receptor gene rearrangement further confirmed the diagnosis of a T-cell pseudolymphoma secondary to ixazomib. Ixazomib is an oral proteasome inhibitor used in the treatment of MM. Other proteasome inhibitors have been reported to trigger cutaneous adverse effects. However, to our knowledge, this is the first report of pseudolymphoma following proteasome inhibitor use. Dermatologists should be aware of this potential effect and the possible management pathways such as cessation and dose reduction.
PubMed: 35663138
DOI: 10.1002/ski2.57 -
Bioengineered Dec 2021To explore the function of transcription factor 3 (TCF3) on the proliferation and apoptosis of Burkitt lymphoma cells and its mechanism. qRT-PCR was performed to...
Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis.
To explore the function of transcription factor 3 (TCF3) on the proliferation and apoptosis of Burkitt lymphoma cells and its mechanism. qRT-PCR was performed to determine the expression of TCF3, histone deacetylase 3 (HDAC3), and microRNA-101 (miR-101) in the Burkitt lymphoma (BL) tumor tissues and lymph node tissues with reactive lymph node hyperplasia (RLNH). We found that the expression of TCF3 and HDAC3 was up-regulated in BL tumor tissues and lymphoma cells, and the miR-101 expression was down-regulated. And TCF3 and HDAC3 were negatively correlated with the expression of miR-101, respectively. In addition, knockdown of TCF3 can inhibit BL cell proliferation, reduce cell viability and promote cell apoptosis, retain the cell cycle in the G0/G1 phase, and inhibit the expression of Akt/mTOR pathway-related proteins (p-Akt and p-mTOR). When miR-101 was overexpressed, the results were the same as when TCF3 was knocked down. Moreover, we used Co-immunoprecipitation (Co-IP) to detect the interaction between TCF3 and HDAC3, and performed the Chromatin immunoprecipitation (ChIP) experiment to detect the enrichment of TCF3 and HDAC3 in the promoter region of miR-101. We found that TCF3 can interact with HDAC3 and is enriched in the miR-101 promoter region. In conclusion, TCF3 combined with HDAC3 down-regulates the expression of miR-101, thereby promoting the proliferation of BL cells and inhibiting their apoptosis.
Topics: Apoptosis; Basic Helix-Loop-Helix Transcription Factors; Burkitt Lymphoma; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Gene Expression Regulation, Neoplastic; Histone Deacetylases; Humans; Lymph Nodes; MicroRNAs; Promoter Regions, Genetic; Pseudolymphoma; Signal Transduction; Up-Regulation
PubMed: 34658308
DOI: 10.1080/21655979.2021.1977557 -
Dermatology (Basel, Switzerland) 2021
Topics: Adult; Aged; Dermoscopy; Female; Humans; Lymphoma; Male; Middle Aged; Pseudolymphoma; Skin Neoplasms
PubMed: 32854093
DOI: 10.1159/000508900 -
Medicine Dec 2022The diagnosis of lymphoma in routine diagnostics can be challenging due to clinical, morphological and immunphenotypical overlap with unusual reactive processes termed...
RATIONALE
The diagnosis of lymphoma in routine diagnostics can be challenging due to clinical, morphological and immunphenotypical overlap with unusual reactive processes termed "pseudolymphomas."
PATIENT CONCERNS
45-year-old male that underwent surgical debridement for a necrotizing fasciitis of the thigh with concomitant excision of a regional lymph node.
DIAGNOSES
The lymph node demonstrated an architecture-effacing activation and proliferation of lymphoblasts and was initially misdiagnosed as an aggressive lymphoma. Only in consideration of the clinical context and with the help of additional immunohistochemical and molecular analyses the final diagnosis of a reactive lymphadenopathy could be made.
INTERVENTIONS
No further therapy was required after the final diagnosis of a reactive lymphadenopathy was made.
OUTCOMES
The clinical follow-up was unremarkable, with no evidence of residual disease after 6 months.
LESSONS
This case report adds the parafollicular activation and proliferation of blasts and plasmablasts in the drainage area of an active infection to the spectrum of "pseudolymphomas" and reiterizes the importance of placing histopathological findings in the proper context.
Topics: Male; Humans; Middle Aged; Fasciitis, Necrotizing; Pseudolymphoma; Lymphoma; Drainage; Debridement; Lymphadenopathy
PubMed: 36595811
DOI: 10.1097/MD.0000000000032457 -
The American Journal of Dermatopathology Sep 2019Immunoglobulin gamma (Ig) type 4-related disease (IgG4-RD) is a chronic immunologic systemic disorder that has gained worldwide recognition in the past decade. This... (Review)
Review
Immunoglobulin gamma (Ig) type 4-related disease (IgG4-RD) is a chronic immunologic systemic disorder that has gained worldwide recognition in the past decade. This entity can affect almost every organ system, and its characteristic lesions have been found in a variety of organs such as lacrimal and salivary glands, pancreas, liver, bile ducts, lungs, kidneys, retroperitoneum, breast, aorta, thyroid, and prostate. This case reports a very rare case of IgG4-RD presenting with a cutaneous lesion in a young female patient mimicking a lymphoproliferative disorder. IgG4-RD affecting the skin is a rare entity and has mostly been reported in Japanese men of middle to older age. IgG4-RD with cutaneous involvement should be in the differential of non-neoplastic, lymphomas, autoimmune, and infectious disorders of the skin.
Topics: Adolescent; Autoimmune Diseases; Biopsy, Needle; Diagnosis, Differential; Female; Humans; Immunoglobulin G4-Related Disease; Immunohistochemistry; Pseudolymphoma; Rare Diseases; Skin Diseases; Thigh; White People
PubMed: 30839348
DOI: 10.1097/DAD.0000000000001401 -
Current Cancer Drug Targets 2021Abnormal spindle-like microcephaly-associated protein (ASPM) has been implicated in the aggressive behavior of several malignant tumors. However, its potential effects...
BACKGROUND
Abnormal spindle-like microcephaly-associated protein (ASPM) has been implicated in the aggressive behavior of several malignant tumors. However, its potential effects on diffuse large B-cell lymphoma (DLBCL) still remain unknown.
METHODS
ASPM levels were determined by immunohistochemically in DLBCL tissues from 54 patients and 15 reactive lymphoid hyperplasia (RLH) tissues as control, and its association with clinical features and overall survival were evaluated. The effects of ASPM on cell growth, cell apoptosis and cell cycle of DLBCL cells were assessed. Bioinformatics, quantitative RT-PCR and western blotting were conducted for mechanic investigation.
RESULTS
ASPM expression was upregulated in DLBCL tissues compared with RLH tissues. Its high expression was correlated with inferior clinicopathological characteristics and poor outcomes of DLBCL patients. Multivariate analysis revealed that high ASPM expression emerged as an independent factor for poor prognosis. In DLBCL cell lines, silencing of ASPM suppressed cell growth, induced cell apoptosis and arrested the cell cycle. Mechanically, effects of ASPM knockdown on DLBCL cells were partially dependent on its block of the Wnt/β-catenin pathway.
CONCLUSION
Collectively, our results suggested that ASPM potentially served as a predictive biomarker of DLCBL tumorigenesis and prognosis, representing a potential therapeutic target for DLCBL.
Topics: Antineoplastic Combined Chemotherapy Protocols; Biomarkers, Tumor; Carcinogenesis; Cell Line, Tumor; Cell Proliferation; Cyclophosphamide; Disease Progression; Doxorubicin; Drug Discovery; Female; Gene Expression Profiling; Gene Knockdown Techniques; Humans; Immunohistochemistry; Lymphoma, Large B-Cell, Diffuse; Male; Middle Aged; Nerve Tissue Proteins; Prednisone; Prognosis; Pseudolymphoma; Vincristine; Wnt Signaling Pathway
PubMed: 32933462
DOI: 10.2174/1568009620666200915090703 -
Revista Espanola de Patologia :... 2019Reactive lymphoid hyperplasia of the liver is an unusual benign lesion of unknown pathogenesis. The largest reported series to date includes only 7 cases. Reactive... (Review)
Review
Reactive lymphoid hyperplasia of the liver is an unusual benign lesion of unknown pathogenesis. The largest reported series to date includes only 7 cases. Reactive lymphoid hyperplasia can be radiologically confused with malignant tumors such as hepatocellular carcinoma or metastases. Histological differential diagnosis should be chiefly made with lymphomas, Castleman disease and primary biliary cirrhosis. We report the clinicopathological findings in a 54-year-old woman with an incidental hepatic lesion when she consulted for hematuria. After histological, immunohistochemical and molecular studies, the diagnosis of reactive lymphoid hyperplasia was made.
Topics: Biomarkers; Carcinoma, Hepatocellular; Castleman Disease; Diagnosis, Differential; Female; Germinal Center; Hematuria; Humans; Incidental Findings; Liver Cirrhosis, Biliary; Liver Neoplasms; Lymphoma; Magnetic Resonance Imaging; Middle Aged; Pseudolymphoma; Tomography, X-Ray Computed; Ultrasonography
PubMed: 31530408
DOI: 10.1016/j.patol.2019.06.001 -
Laboratory Investigation; a Journal of... Oct 2020A pathological evaluation is one of the most important methods for the diagnosis of malignant lymphoma. A standardized diagnosis is occasionally difficult to achieve...
A pathological evaluation is one of the most important methods for the diagnosis of malignant lymphoma. A standardized diagnosis is occasionally difficult to achieve even by experienced hematopathologists. Therefore, established procedures including a computer-aided diagnosis are desired. This study aims to classify histopathological images of malignant lymphomas through deep learning, which is a computer algorithm and type of artificial intelligence (AI) technology. We prepared hematoxylin and eosin (H&E) slides of a lesion area from 388 sections, namely, 259 with diffuse large B-cell lymphoma, 89 with follicular lymphoma, and 40 with reactive lymphoid hyperplasia, and created whole slide images (WSIs) using a whole slide system. WSI was annotated in the lesion area by experienced hematopathologists. Image patches were cropped from the WSI to train and evaluate the classifiers. Image patches at magnifications of ×5, ×20, and ×40 were randomly divided into a test set and a training and evaluation set. The classifier was assessed using the test set through a cross-validation after training. The classifier achieved the highest levels of accuracy of 94.0%, 93.0%, and 92.0% for image patches with magnifications of ×5, ×20, and ×40, respectively, in comparison to diffuse large B-cell lymphoma, follicular lymphoma, and reactive lymphoid hyperplasia. Comparing the diagnostic accuracies between the proposed classifier and seven pathologists, including experienced hematopathologists, using the test set made up of image patches with magnifications of ×5, ×20, and ×40, the best accuracy demonstrated by the classifier was 97.0%, whereas the average accuracy achieved by the pathologists using WSIs was 76.0%, with the highest accuracy reaching 83.3%. In conclusion, the neural classifier can outperform pathologists in a morphological evaluation. These results suggest that the AI system can potentially support the diagnosis of malignant lymphoma.
Topics: Algorithms; Deep Learning; Diagnosis, Computer-Assisted; Histological Techniques; Humans; Image Interpretation, Computer-Assisted; Lymphoma; Lymphoma, Follicular; Lymphoma, Large B-Cell, Diffuse; Neural Networks, Computer; Observer Variation; Pathologists; Pseudolymphoma
PubMed: 32472096
DOI: 10.1038/s41374-020-0442-3 -
Annales de Dermatologie Et de... Dec 2022
Topics: Humans; Tattooing; Pseudolymphoma; Skin Neoplasms; Lymphoma
PubMed: 35644693
DOI: 10.1016/j.annder.2022.03.004 -
International Journal of Dermatology Jun 2022
Topics: Humans; Immunologic Factors; Pseudolymphoma; Skin Diseases; Tumor Necrosis Factor-alpha
PubMed: 34242411
DOI: 10.1111/ijd.15760