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Systematic and Applied Microbiology May 2021In a study carried out between 2013 and 2018 in fish farms in Turkey, several putative novel species were isolated. The 16S rRNA nucleotide sequences of fourteen strains...
In a study carried out between 2013 and 2018 in fish farms in Turkey, several putative novel species were isolated. The 16S rRNA nucleotide sequences of fourteen strains of Gram-negative rods, which were isolated from asymptomatic and symptomatic rainbow trouts (Onchorhynchus mykiss), placed them under the genus Pseudomonas. The similarity values of the concatenated nucleotide sequences of the rpoD, rpoB, gyrB and 16S rRNA genes clustered these isolates into the P. fluorescens phylogenetic group of species and into the Pseudomonas koreensis subgroup, close to Pseudomonas helmanticensis and Pseudomonas baetica. An isolate of a totally different origin, strain CCUG 67011, clustered with these isolates. Phenotypic characterization, together with the chemotaxonomic data, whole-cell MALDI-TOF MS and fatty acids methyl esters analyses were performed. The DNA G + C content was 58.7 mol% for isolate P9 and 58.8 mol% for isolate P42. The phylogenomic analysis and whole genome nucleotide sequences of four of these isolates confirmed that the isolates P9, P25 and P141, represent a novel species for which the name Pseudomonas anatoliensis sp. nov. is proposed, with P9 as the type strain (=CCUG 74755 = CECT 3172). The isolates P1, P2, P10, P27, P30, P24a, P42, P117, P139, P152 and CCUG 67011 represent another novel sècies, for which the name Pseudomonas iridis sp. nov. is proposed, with P42 as the type strain (=CCUG 74870 = CECT 3174).
Topics: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Genes, Bacterial; Nucleic Acid Hybridization; Oncorhynchus mykiss; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Turkey
PubMed: 33838437
DOI: 10.1016/j.syapm.2021.126198 -
Angewandte Chemie (International Ed. in... Mar 2020Butenolides are well-known signaling molecules in Gram-positive bacteria. Here, we describe a novel class of butenolides isolated from a Gram-negative Pseudomonas...
Butenolides are well-known signaling molecules in Gram-positive bacteria. Here, we describe a novel class of butenolides isolated from a Gram-negative Pseudomonas strain, the styrolides. Structure elucidation was aided by the total synthesis of styrolide A. Transposon mutagenesis enabled us to identify the styrolide biosynthetic gene cluster, and by using a homology search, we discovered the related and previously unknown acaterin biosynthetic gene cluster in another Pseudomonas species. Mutagenesis, heterologous expression, and identification of key shunt and intermediate products were crucial to propose a biosynthetic pathway for both Pseudomonas-derived butenolides. Comparative transcriptomics suggests a link between styrolide formation and the regulatory networks of the bacterium.
Topics: 4-Butyrolactone; Bacterial Proteins; DNA Transposable Elements; Multigene Family; Mutagenesis; Pseudomonas; Soil Microbiology
PubMed: 31880848
DOI: 10.1002/anie.201914154 -
Archives of Microbiology Jul 2021Insects and bacteria are the most widespread groups of organisms found in nearly all habitats on earth, establishing diverse interactions that encompass the entire range... (Review)
Review
Insects and bacteria are the most widespread groups of organisms found in nearly all habitats on earth, establishing diverse interactions that encompass the entire range of possible symbiotic associations from strict parasitism to obligate mutualism. The complexity of their interactions is instrumental in shaping the roles of insects in the environment, meanwhile ensuring the survival and persistence of the associated bacteria. This review aims to provide detailed insight on the multifaceted symbiosis between one of the most versatile bacterial genera, Pseudomonas (Gammaproteobacteria: Pseudomonadaceae) and a diverse group of insect species. The Pseudomonas engages with varied interactions with insects, being either a pathogen or beneficial endosymbiont, as well as using insects as vectors. In addition, this review also provides updates on existing and potential applications of Pseudomonas and their numerous insecticidal metabolites as biocontrol agents against pest insects for the improvement of integrated pest management strategies. Here, we have summarized several known modes of action and the virulence factors of entomopathogenic Pseudomonas strains essential for their pathogenicity against insects. Meanwhile, the beneficial interactions between pseudomonads and insects are currently limited to a few known insect taxa, despite numerous studies reporting identification of pseudomonads in the guts and haemocoel of various insect species. The vector-symbiont association between pseudomonads and insects can be diverse from strict phoresy to a role switch from commensalism to parasitism following a dose-dependent response. Overall, the pseudomonads appeared to have evolved independently to be either exclusively pathogenic or beneficial towards insects.
Topics: Animals; Biological Control Agents; Host-Pathogen Interactions; Insect Vectors; Insecta; Pseudomonas; Symbiosis; Virulence Factors
PubMed: 33634321
DOI: 10.1007/s00203-021-02230-9 -
Microbial Cell Factories Jun 2023A subject of great interest is the bioprospecting of microorganisms and their bioactive byproducts, such as pigments. Microbial pigments have various benefits, including... (Review)
Review
A subject of great interest is the bioprospecting of microorganisms and their bioactive byproducts, such as pigments. Microbial pigments have various benefits, including being safe to use due to their natural makeup, having therapeutic effects, and being produced all year round, regardless of the weather or location. Pseudomonas aeruginosa produces phenazine pigments that are crucial for interactions between Pseudomonas species and other living things. Pyocyanin pigment, which is synthesized by 90-95% of P. aeruginosa, has potent antibacterial, antioxidant, and anticancer properties. Herein, we will concentrate on the production and extraction of pyocyanin pigment and its biological use in different areas of biotechnology, engineering, and biology.
Topics: Pyocyanine; Pseudomonas aeruginosa; Pseudomonas; Anti-Bacterial Agents; Antioxidants
PubMed: 37291560
DOI: 10.1186/s12934-023-02122-1 -
Microbiology (Reading, England) Jan 2020
Topics: Biological Transport; Humans; Microbial Interactions; Microbiology; Periodicals as Topic; Pseudomonas; Saccharomyces cerevisiae
PubMed: 32003323
DOI: 10.1099/mic.0.000882 -
Science (New York, N.Y.) Mar 2021Microbial production of antibiotics is common, but our understanding of their roles in the environment is limited. In this study, we explore long-standing observations...
Microbial production of antibiotics is common, but our understanding of their roles in the environment is limited. In this study, we explore long-standing observations that microbes increase the production of redox-active antibiotics under phosphorus limitation. The availability of phosphorus, a nutrient required by all life on Earth and essential for agriculture, can be controlled by adsorption to and release from iron minerals by means of redox cycling. Using phenazine antibiotic production by pseudomonads as a case study, we show that phenazines are regulated by phosphorus, solubilize phosphorus through reductive dissolution of iron oxides in the lab and field, and increase phosphorus-limited microbial growth. Phenazines are just one of many examples of phosphorus-regulated antibiotics. Our work suggests a widespread but previously unappreciated role for redox-active antibiotics in phosphorus acquisition and cycling.
Topics: Anti-Bacterial Agents; Batch Cell Culture Techniques; Biological Availability; Oxidation-Reduction; Phenazines; Phosphorus; Pseudomonas
PubMed: 33674490
DOI: 10.1126/science.abd1515 -
International Journal of Systematic and... Nov 2021Two Gram-staining-negative, aerobic, rod-shaped bacteria designated strains SR9 and UL070, were isolated from soil and subjected to taxonomic characterization. Strain...
Two Gram-staining-negative, aerobic, rod-shaped bacteria designated strains SR9 and UL070, were isolated from soil and subjected to taxonomic characterization. Strain SR9 grew at 10-37 °C (optimum 30 °C), at pH 4.0-10.0 (optimum pH 8.0) and in the presence of 0-1 % NaCl (optimum 0 %), and UL070 at 4-33 °C (optimum 30 °C), at pH 4.0-10.0 (optimum pH 7.0) and in the presence of 0-2 % NaCl (optimum 0 %), respectively. Strain UL070 was motile with flagella. Analysis of 16S rRNA gene sequences indicated that the two strains fell into phylogenetic clusters belonging to the genus . Both strains SR9 and UL070 were mostly related to S1-A32-2 with 99.70 and 99.01% sequence similarities, and the similarity between the two isolates was 98.90 %. The genome-based analyses indicated that each of the strains SR9 and UL070 was clearly separated from other species of , as the orthologous average nucleotide identity (OrthoANI) and the digital DNA-DNA hybridization (dDDH) values were no higher than 93.09 and 50.03% respectively with any related species, which were clearly below the cutoff for species distinction. The fatty acid profiles of the two strains mainly consisting of unsaturated components, the presence of ubiquinone 9 (Q-9) as the major respiratory quinone, and phosphatidylethanolamine (PE) and diphosphatidylglycerol (DPG) as the diagnostic polar lipids were consistent with their classification into . The DNA G+C contents of strains SR9 and UL070 were 63.2 mol% and 63.6 mol% respectively. On the basis of both phenotypic and phylogenetic evidences, each of the isolated strains should be classified as a novel species, for which the names sp. nov. (type strain=SR9=KCTC 82228=JCM 34509) and sp. nov. (type strain=UL070=KCTC 82229=JCM 34510) are proposed.
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Nucleic Acid Hybridization; Phospholipids; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone
PubMed: 34767499
DOI: 10.1099/ijsem.0.005082 -
Critical Reviews in Microbiology Aug 2020Lipopeptides (LPs) are a prominent class of molecules among the steadily growing spectrum of specialized metabolites retrieved from , in particular soil-dwelling and... (Review)
Review
Lipopeptides (LPs) are a prominent class of molecules among the steadily growing spectrum of specialized metabolites retrieved from , in particular soil-dwelling and plant-associated isolates. Among the multiple LP families, pioneering research focussed on phytotoxic and antimicrobial cyclic lipopeptides (CLPs) of the ubiquitous plant pathogen (syringomycin and syringopeptin). Their non-ribosomal peptide synthetases (NRPSs) are embedded in biosynthetic gene clusters (BGCs) that are tightly co-clustered on a pathogenicity island. Other members of the group () and some species of the group and complex have adopted these biosynthetic strategies to co-produce their own mycin and peptin variants, in some strains supplemented with an analogue of the linear LP (LLP), syringafactin. This capacity is not confined to phytopathogens but also occurs in some biocontrol strains, which indicates that these LP families not solely function as general virulence factors. We address this issue by scrutinizing the structural diversity and bioactivities of LPs from the mycin, peptin, and factin families in a phylogenetic and evolutionary perspective. BGC functional organization (including associated regulatory and transport genes) and NRPS modular architectures in known and candidate LP producers were assessed by genome mining.
Topics: Bacterial Proteins; Lipopeptides; Peptide Synthases; Phylogeny; Plant Diseases; Plants; Pseudomonas
PubMed: 32885723
DOI: 10.1080/1040841X.2020.1794790 -
International Journal of Molecular... Sep 2022Pyoverdines (PVDs) are a class of siderophores produced mostly by members of the genus . Their primary function is to accumulate, mobilize, and transport iron necessary... (Review)
Review
Pyoverdines (PVDs) are a class of siderophores produced mostly by members of the genus . Their primary function is to accumulate, mobilize, and transport iron necessary for cell metabolism. Moreover, PVDs also play a crucial role in microbes' survival by mediating biofilm formation and virulence. In this review, we reorganize the information produced in recent years regarding PVDs biosynthesis and pathogenic mechanisms, since PVDs are extremely valuable compounds. Additionally, we summarize the therapeutic applications deriving from the PVDs' use and focus on their role as therapeutic target themselves. We assess the current biotechnological applications of different sectors and evaluate the state-of-the-art technology relating to the use of synthetic biology tools for pathway engineering. Finally, we review the most recent methods and techniques capable of identifying such molecules in complex matrices for drug-discovery purposes.
Topics: Iron; Oligopeptides; Pseudomonas; Pseudomonas aeruginosa; Siderophores
PubMed: 36232800
DOI: 10.3390/ijms231911507 -
Molecular Microbiology Aug 2019Pseudomonas bacteria are widespread and are found in soil and water, as well as pathogens of both plants and animals. The ability of Pseudomonas to colonize many... (Review)
Review
Pseudomonas bacteria are widespread and are found in soil and water, as well as pathogens of both plants and animals. The ability of Pseudomonas to colonize many different environments is facilitated by the multiple signaling systems these bacteria contain that allow Pseudomonas to adapt to changing circumstances by generating specific responses. Among others, signaling through extracytoplasmic function σ (σ ) factors is extensively present in Pseudomonas. σ factors trigger expression of functions required under particular conditions in response to specific signals. This manuscript reviews the phylogeny and biological roles of σ factors in Pseudomonas, and highlights the diversity of σ -signaling pathways of this genus in terms of function and activation. We show that Pseudomonas σ factors belong to 16 different phylogenetic groups. Most of them are included within the iron starvation group and are mainly involved in iron acquisition. The second most abundant group is formed by RpoE-like σ factors, which regulate the responses to cell envelope stress. Other groups controlling solvent tolerance, biofilm formation and the response to oxidative stress, among other functions, are present in lower frequency. The role of σ factors in the virulence of Pseudomonas pathogenic species is described.
Topics: Animals; Bacterial Proteins; Extracellular Space; Gene Expression Regulation, Bacterial; Humans; Phylogeny; Pseudomonas; Pseudomonas Infections; Sigma Factor; Signal Transduction
PubMed: 31206859
DOI: 10.1111/mmi.14331