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Trends in Genetics : TIG Dec 2019
Topics: Genome, Fungal; Genomics; Saccharomyces cerevisiae
PubMed: 31630852
DOI: 10.1016/j.tig.2019.08.009 -
Biotechnology Journal Sep 2019For thousands of years, the yeast Saccharomyces cerevisiae (S. cerevisiae) has served as a cell factory for the production of bread, beer, and wine. In more recent... (Review)
Review
For thousands of years, the yeast Saccharomyces cerevisiae (S. cerevisiae) has served as a cell factory for the production of bread, beer, and wine. In more recent years, this yeast has also served as a cell factory for producing many different fuels, chemicals, food ingredients, and pharmaceuticals. S. cerevisiae, however, has also served as a very important model organism for studying eukaryal biology, and even today many new discoveries, important for the treatment of human diseases, are made using this yeast as a model organism. Here a brief review of the use of S. cerevisiae as a model organism for studying eukaryal biology, its use as a cell factory, and how advances in systems biology underpin developments in both these areas, is provided.
Topics: Metabolic Engineering; Saccharomyces cerevisiae; Synthetic Biology; Systems Biology
PubMed: 30925027
DOI: 10.1002/biot.201800421 -
International Journal of Molecular... Oct 2022The yeast has been used for bread making and beer brewing for thousands of years. In addition, its ease of manipulation, well-annotated genome, expansive molecular... (Review)
Review
The yeast has been used for bread making and beer brewing for thousands of years. In addition, its ease of manipulation, well-annotated genome, expansive molecular toolbox, and its strong conservation of basic eukaryotic biology also make it a prime model for eukaryotic cell biology and genetics. In this review, we discuss the characteristics that made yeast such an extensively used model organism and specifically focus on the DNA damage response pathway as a prime example of how research in helped elucidate a highly conserved biological process. In addition, we also highlight differences in the DNA damage response of and humans and discuss the challenges of using as a model system.
Topics: Biological Phenomena; Biology; Cell Cycle Checkpoints; DNA Damage; Eukaryotic Cells; Humans; Saccharomyces cerevisiae
PubMed: 36232965
DOI: 10.3390/ijms231911665 -
Yeast (Chichester, England) Jan 2020
Topics: Lipids; Saccharomyces cerevisiae
PubMed: 31943346
DOI: 10.1002/yea.3459 -
Cell Mar 2024Cell cycle progression relies on coordinated changes in the composition and subcellular localization of the proteome. By applying two distinct convolutional neural...
Cell cycle progression relies on coordinated changes in the composition and subcellular localization of the proteome. By applying two distinct convolutional neural networks on images of millions of live yeast cells, we resolved proteome-level dynamics in both concentration and localization during the cell cycle, with resolution of ∼20 subcellular localization classes. We show that a quarter of the proteome displays cell cycle periodicity, with proteins tending to be controlled either at the level of localization or concentration, but not both. Distinct levels of protein regulation are preferentially utilized for different aspects of the cell cycle, with changes in protein concentration being mostly involved in cell cycle control and changes in protein localization in the biophysical implementation of the cell cycle program. We present a resource for exploring global proteome dynamics during the cell cycle, which will aid in understanding a fundamental biological process at a systems level.
Topics: Eukaryotic Cells; Neural Networks, Computer; Proteome; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
PubMed: 38452761
DOI: 10.1016/j.cell.2024.02.014 -
ELife Nov 2022A new pooled screening method in yeast allows scientists to probe how protein levels are regulated by mutating thousands of genes at once.
A new pooled screening method in yeast allows scientists to probe how protein levels are regulated by mutating thousands of genes at once.
Topics: Gene Expression Regulation, Fungal; Saccharomyces cerevisiae
PubMed: 36326804
DOI: 10.7554/eLife.83907 -
Microbiology (Reading, England) Jan 2020
Topics: Biological Transport; Humans; Microbial Interactions; Microbiology; Periodicals as Topic; Pseudomonas; Saccharomyces cerevisiae
PubMed: 32003323
DOI: 10.1099/mic.0.000882 -
FEMS Yeast Research Sep 2020
Topics: Metabolic Engineering; Saccharomyces cerevisiae; Synthetic Biology
PubMed: 32854113
DOI: 10.1093/femsyr/foaa049 -
Biological Chemistry May 2020Mitochondria are essential organelles of virtually all eukaryotic organisms. As they cannot be made de novo, they have to be inherited during cell division. In this... (Review)
Review
Mitochondria are essential organelles of virtually all eukaryotic organisms. As they cannot be made de novo, they have to be inherited during cell division. In this review, we provide an overview on mitochondrial inheritance in Saccharomyces cerevisiae, a powerful model organism to study asymmetric cell division. Several processes have to be coordinated during mitochondrial inheritance: mitochondrial transport along the actin cytoskeleton into the emerging bud is powered by a myosin motor protein; cell cortex anchors retain a critical fraction of mitochondria in the mother cell and bud to ensure proper partitioning; and the quantity of mitochondria inherited by the bud is controlled during cell cycle progression. Asymmetric division of yeast cells produces rejuvenated daughter cells and aging mother cells that die after a finite number of cell divisions. We highlight the critical role of mitochondria in this process and discuss how asymmetric mitochondrial partitioning and cellular aging are connected.
Topics: Asymmetric Cell Division; Mitochondria; Saccharomyces cerevisiae
PubMed: 31967958
DOI: 10.1515/hsz-2019-0439 -
Applied Microbiology and Biotechnology Mar 2022Glutathione (L-γ-glutamyl-cysteinyl-glycine, GSH) is a tripeptide synthesized through consecutive enzymatic reactions. Among its several metabolic functions in cells,... (Review)
Review
Glutathione (L-γ-glutamyl-cysteinyl-glycine, GSH) is a tripeptide synthesized through consecutive enzymatic reactions. Among its several metabolic functions in cells, the main one is the potential to act as an endogenous antioxidant agent. GSH has been the focus of numerous studies not only due to its role in the redox status of biological systems but also due to its biotechnological characteristics. GSH is usually obtained by fermentation and shows a variety of applications by the pharmaceutical and food industry. Therefore, the search for new strategies to improve the production of GSH during fermentation is crucial. This mini review brings together recent papers regarding the principal parameters of the biotechnological production of GSH by Saccharomyces cerevisiae. In this context, aspects, such as the medium composition (amino acids, alternative raw materials) and the use of technological approaches (control of osmotic and pressure conditions, magnetic field (MF) application, fed-batch process) were considered, along with genetic engineering knowledge, trends, and challenges in viable GSH production. KEY POINTS: • Saccharomyces cerevisiae has shown potential for glutathione production. • Improved technological approaches increases glutathione production. • Genetic engineering in Saccharomyces cerevisiae improves glutathione production.
Topics: Biotechnology; Fermentation; Genetic Engineering; Glutathione; Saccharomyces cerevisiae
PubMed: 35182192
DOI: 10.1007/s00253-022-11826-0