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Talanta Jun 2023In reality, various sulfonamides (SAs) were alternately used in animal husbandry to avoid generating drug resistance. Thus, it is crucial to develop simple and...
In reality, various sulfonamides (SAs) were alternately used in animal husbandry to avoid generating drug resistance. Thus, it is crucial to develop simple and high-throughput methods for detecting multiple or groups of SAs to realize rapid screening of total SAs residues in foods. We herein developed a sensitive and efficient MnO nanosheets-mediated etching of gold nanobipyramids (AuNBPs), which can generate more vivid color changes, and further fabricated a high-throughput multicolor immunosensor for the visual screening/semi-quantitative detection of 6 different SAs including sulfamethazine (SMZ), sulfamethoxydiazine (SMD), sulfisomidine (SIM), sulfamerazine (SMR), sulfamonomethoxine (SMM) and sulfaquinoxaline (SQ) by using AuNBPs as signal and broad-specificity anti-SAs antibody as a bio-receptor. The immunosensor displays more vivid color changes, and has a lower visual detection limit and excellent specificity. It can be applied to detect as little as 1.0 ng/mL of SMZ, SMD, SMR and 2.0 ng/mL of SIM, SMM, SQ by bare eye observation, and 0.2 ng/mL of above 6 SAs by UV-visible spectrophotometry. The visual detection limit of the immunosensor is much lower than the maximum residue limit of total SAs (100 μg/kg) in edible tissues. The immunosensor was successfully applied to detect SMZ, SMD, SIM, SMR, SMM and SQ in milk with a recovery of 84%-106% and a RSD (n = 5) < 8%. The success of this study provided a promising assay for the on-site rapid screening of SMZ, SMD, SIM, SMR, SMM and SQ in food by bare eye observation. Importantly, the immunosensor may be expended as a general method for the visual screening/semi-quantitative detection of the group of other antibiotics by using the corresponding broad-specificity antibody as a bio-receptor.
Topics: Animals; Sulfonamides; Manganese Compounds; Oxides; Gold; Biosensing Techniques; Immunoassay; Sulfanilamide
PubMed: 36924640
DOI: 10.1016/j.talanta.2023.124449 -
Journal of the American Chemical Society Oct 2022Sulfoximines are increasingly incorporated in agrochemicals and pharmaceuticals, with the two enantiomers of chiral sulfoximines often having profoundly different...
Sulfoximines are increasingly incorporated in agrochemicals and pharmaceuticals, with the two enantiomers of chiral sulfoximines often having profoundly different binding interactions with biomolecules. Therefore, their application to drug discovery and development requires the challenging preparation of single enantiomers rather than racemic mixtures. Here, we report a general and fundamentally new asymmetric synthesis of sulfoximines. The first -alkylation of sulfenamides, which are readily accessible sulfur compounds with one carbon and one nitrogen substituent, represents the key step. A broad scope for -alkylation was achieved by rhodium-catalyzed coupling with diazo compounds under mild conditions. When a chiral rhodium catalyst was utilized with loadings as low as 0.1 mol %, the -alkylation products were obtained in high yields and with enantiomeric ratios up to 98:2 at the newly generated chiral sulfur center. The -alkylation products were efficiently converted to a variety of sulfoximines with complete retention of stereochemistry. The utility of this approach was further demonstrated by the asymmetric synthesis of a complex sulfoximine agrochemical.
Topics: Agrochemicals; Alkylation; Carbon; Catalysis; Molecular Structure; Nitrogen; Pharmaceutical Preparations; Rhodium; Stereoisomerism; Sulfamerazine; Sulfur
PubMed: 36154032
DOI: 10.1021/jacs.2c09158 -
Journal of Environmental Sciences... Nov 2020Sulfonamides (SAs) are common antimicrobial drugs, which are frequently detected in surface water systems, and are difficult to degrade, posing a potential threat to the...
Sulfonamides (SAs) are common antimicrobial drugs, which are frequently detected in surface water systems, and are difficult to degrade, posing a potential threat to the aquatic environment. However, little is known about the potential adverse effects of SAs on non-target organisms (e.g., microalgae) in the aquatic ecosystem. In this study, the effect of SAs (sulfadiazine (SD), sulfamerazine (SM1), and sulfamethazine (SM2) at 1, 5, 20, and 50 mg/L concentrations, respectively) on the freshwater microalga Dictyosphaerium sp. was investigated, with respect to changes of biomass and chlorophyll a content and induction of extracellular polymer substances (EPS), including protein and polysaccharide contents. At the same time, the residue of SAs was determined. The results showed that Dictyosphaerium sp. was tolerant to the three SAs, and the chlorophyll a content in Dictyosphaerium sp. significantly decreased on day 7, followed by a "compensation phenomena". The increase in protein and polysaccharide contents played a defensive role in Dictyosphaerium sp. against antibiotic stress, and there was a strong positive correlation between polysaccharide contents and antibiotic concentrations. Dictyosphaerium sp. exhibited 35%-45%, 30%-42%, and 26%-51% removal of SD, SM1, and SM2, respectively. This study is helpful to understand the changes of EPS in the defense process of microalgae under the action of antibiotics, and provides a new insight for the ecological removal of antibiotic pollution in natural surface water system.
Topics: Chlorophyll A; Ecosystem; Fresh Water; Microalgae; Sulfonamides
PubMed: 32933729
DOI: 10.1016/j.jes.2020.05.018 -
Journal of Hazardous Materials Jan 2021To improve the visible-light photocatalytic activity of graphitic carbon nitrate (g-CN) for practical application, a GdO-cluster decorated O-doping g-CN was fabricated...
To improve the visible-light photocatalytic activity of graphitic carbon nitrate (g-CN) for practical application, a GdO-cluster decorated O-doping g-CN was fabricated via an ethanol assisted solvothermal-polycondensation method. The as-prepared photocatalysts, including bulk g-CN (CN), O-doping g-CN (HECN) and GdO-cluster decorated O-doping g-CN (HECN-xGd), were characterized and the paralleled experiments were conducted to evaluate the photocatalytic activity, mineralization capacity and mineralization mechanism, where sulfamerazine (SMR) was employed as the target pollutant. Furthermore, the quenching tests with scavengers were executed to analyze the contributions of the dominant active species, where the O was identified as a major role, and h as the minor role in the photodegradation of SMR. Results from the paralleled experiments suggested that the HECN-xGd possesses superior photocatalytic activity to HECN and CN, besides the feasible reusability through five cycles and impressive total organic carbon (TOC) removal about 60%. And the improved photocatalytic activity of HECN-xGd is ascribed mainly to the oxygen doping and GdO decoration. Herein, oxygen doping optimizes the structure of g-CN and expands the light absorption range of HECN, and GdO facilitates the reduction of O into O, and acts as the separator and transporter for the photo-induced charges.
PubMed: 33254791
DOI: 10.1016/j.jhazmat.2020.123780 -
The Science of the Total Environment Jun 2024To obtain a multifunctional bacterium that can effectively degrade polyethylene (PE) and sulfonamide antibiotics (SAs), PE and SAs were selected as the primary research...
To obtain a multifunctional bacterium that can effectively degrade polyethylene (PE) and sulfonamide antibiotics (SAs), PE and SAs were selected as the primary research objects. Multifunctional degrading bacteria were isolated and screened from an environment in which plastics and antibiotics have existed for a long time. An efficient degrading strain, Raoultella sp., was screened by measuring the degradation performance of PE and SAs. We analyzed the changes in the microbial community of indigenous bacteria using 16S rRNA. After 60 d of degradation at 28 °C, the Raoultella strain to PE degradation rate was 4.20 %. The SA degradation rates were 96 % (sulfonathiazole, (ST)), 86 % (sulfamerazine, (SM)), 72 % (sulfamethazine, (SM2)) and 64 % (sulfamethoxazole, (SMX)), respectively. This bacterium increases the surface roughness of PE plastic films and produces numerous gullies, pits, and folds. In addition, after 60 d, the contact angle of the plastic film decreased from 92.965° to 70.205°, indicating a decrease in hydrophobicity. High-throughput sequencing analysis of the degrading bacteria revealed that the Raoultella strain encodes enzymes involved in PE and SA degradation. The results of this study not only provide a theoretical basis for further study of the degradation mechanism of multifunctional and efficient degrading bacteria but also provide potential strain resources for the biodegradation of waste plastics and antibiotics in the environment.
Topics: Biodegradation, Environmental; Anti-Bacterial Agents; Soil Pollutants; Polyethylene; Soil Microbiology; RNA, Ribosomal, 16S; Soil; Bacteria
PubMed: 38649045
DOI: 10.1016/j.scitotenv.2024.172619 -
Heliyon Dec 2021This article describes the initial study on the simultaneous determination of multiclass antibiotic residues in imported and local frozen poultry specimens, including...
Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection.
This article describes the initial study on the simultaneous determination of multiclass antibiotic residues in imported and local frozen poultry specimens, including turkey gizzard and muscle tissues, and chicken muscle tissues, commonly consumed in Ogun State, Nigeria. Minced tissues were treated with phosphate buffer adjusted to pH 7 that was cleaned using C18 SPE-column (Supelclean™) cartridge. For the determination of six antibiotic residues including fluoroquinolones, sulfonamides, and macrolides, a solid-phase extraction method was used, followed by extract analysis using high-performance liquid chromatography-diode array detection (HPLC-DAD). The coefficient of determination (R) for the external standards for all the analytes ranged between 0.963 and 0.999. The limit of detection (LOD) and quantification (LOQ) ranged between 5.37 - 55.4 μg/kg, and 17.9-185 μg/kg, respectively. Enrofloxacin, sulfadimethoxine, sulfamerazine, and tylosin showed high concentration levels in the frozen poultry beyond acceptable maximum residue limits (MRLs). The six drugs considered in this study were present at higher concentrations in domestic chicken tissues than the permissible level. This suggests that farmers do not observe the cessation period before poultry birds previously treated with antibiotics are sold to consumers thus exposing them to potentially hazardous antibiotic residues.
PubMed: 34917790
DOI: 10.1016/j.heliyon.2021.e08469 -
Journal of Chromatography. A Aug 2021Liquid phase microextraction (LPME) into a microfluidic has undergone great advances focused on downscaled and miniaturized devices. In this work, a microfluidic device...
Liquid phase microextraction (LPME) into a microfluidic has undergone great advances focused on downscaled and miniaturized devices. In this work, a microfluidic device was developed for the extraction of sulfonamides in order to accelerate the mass transfer and passive diffusion of the analytes from the donor phase to the acceptor phase. The subsequent analysis was carried out by high performance liquid chromatography with UV-DAD (HPLC-DAD). Several parameters affecting the extraction efficiency of the method such as the supported liquid membrane, composition of donor and acceptor phase and flow rate were investigated and optimized. Tributyl phosphate was found to be a good supported liquid membrane which confers not only great affinity for analytes but also long-term stability, allowing more than 20 consecutive extractions without carry over effect. Under optimum conditions, extraction efficiencies were over 96 % for all sulfonamides after 10 minutes extraction and only 10 µL of sample was required. Relative standard deviation was between 3-5 % for all compounds. Method detection limits were 45, 57, 54 and 33 ng mL for sulfadiazine (SDI), sulfamerazine (SMR), sulfamethazine (SMT) and sulfamethoxazole (SMX), respectively. Quantitation limits were 0.15, 0.19, 0.18 and 0.11 µg mL for SDI, SMR, SMT SMX, respectively. The proposed microfluidic device was successfully applied for the determination of sulfonamides in urine samples with extraction efficiencies within the range of 86-106 %. The proposed method improves the procedures proposed to date for the determination of sulfonamides in terms of efficiency, reduction of the sample volume and extraction time.
Topics: Chromatography, High Pressure Liquid; Humans; Lab-On-A-Chip Devices; Liquid Phase Microextraction; Microfluidics; Sulfonamides; Urinalysis
PubMed: 34186325
DOI: 10.1016/j.chroma.2021.462344 -
Biosensors & Bioelectronics Aug 2024Environmental antibiotics and antibiotic resistance genes (ARGs) pose considerable threat to humans and animals; thus, the rapid and sensitive parallel detection of...
Rapid and sensitive parallel on-site detection of antibiotics and resistance genes in aquatic environments using evanescent wave dual-color fluorescence fiber-embedded optofluidic nanochip.
Environmental antibiotics and antibiotic resistance genes (ARGs) pose considerable threat to humans and animals; thus, the rapid and sensitive parallel detection of these pollutants from a single sample is urgently required. However, traditional multiplexed analytic technologies detect only one type of target (e.g., small molecules or nucleic acids) per assay. To address this issue, Evanescent wave Dual-color fluorescence Fiber-embedded Optofluidic Nanochip (EDFON) was fabricated by integrating a fiber-embedded optofluidic nanochip with evanescent wave dual-color fluorescence technology. The EDFON was used for the parallel quantitative detection of sulfamerazine (SMR) and MCR-1 with high sensitivity and specificity by combining a heterogeneous immunoassay with a homogenous hybridization chain reaction based on time-resolved effects. LODs of 0.032 μg/L and 35 pM was obtained for SMR and MCR-1, respectively, within 20 min. To our best knowledge, the EDFON is the first device for the simultaneous detection of two type of targets in each test, which is highly valuable to prevent the global threats of antibiotics and ARGs. Comparison with liquid chromatography-mass spectrometry showed a strong linear relationship (R = 0.998) for SMR pollution in the Qinghe River, with spiked SMR and MCR-1 negative surface and wastewater samples showing recovery rates of 91.8-113.4%. These results demonstrate the excellent accuracy and reliability of the EDFON, with features such as multi-analyte detection, field-deployment, and minimal-equipment, rendering it revolutionary for environmental monitoring, food safety, and medical diagnostics.
Topics: Biosensing Techniques; Anti-Bacterial Agents; Water Pollutants, Chemical; Limit of Detection; Drug Resistance, Microbial; Spectrometry, Fluorescence; Equipment Design; Fluorescence
PubMed: 38677021
DOI: 10.1016/j.bios.2024.116281 -
Environmental Science and Pollution... Nov 2019Most sulfonamides, widely used around the world, are excreted via feces and urine along with their metabolites in humans and animals. Therefore, understanding the...
Most sulfonamides, widely used around the world, are excreted via feces and urine along with their metabolites in humans and animals. Therefore, understanding the potential removal pathway of sulfonamides and their metabolites in wastewater treatment systems is of importance. The occurrence and fate of four sulfonamides and their acetyl metabolites in wastewater and sludge in a biological aerated filter in Xiamen city were evaluated. Six of the target compounds were detected in wastewater, but only parent compounds were detected in sludge. The highest concentration in wastewater was acetyl-sulfamethoxazole (Ac-SMZ) with a concentration of 75.2 ng/L. Removal efficiency and mass load in wastewater treatment systems were calculated. In terms of the overall removal efficiency, they ranged from 24.4 to 100%. The removal efficiencies of sulfamerazine (SM1), sulfamethazine (SM2), and sulfadiazine (SD) were up to 100% while N-acetyl sulfamerazine (Ac-SM1) showed the lowest removal efficiency. Biodegradation was the dominant remove pathway according to the mass balance analysis while SD and SM2 were sludge adsorption. The results can provide an insight into the fate of target sulfonamides in BAF systems and provide data to assess their potential ecological risks.
Topics: Adsorption; Animals; Biodegradation, Environmental; China; Humans; Sewage; Sulfadiazine; Sulfamethazine; Sulfonamides; Waste Disposal, Fluid; Wastewater; Water Pollutants, Chemical
PubMed: 31522397
DOI: 10.1007/s11356-019-06311-7 -
Environmental Technology Jul 2024Natural organic matter (NOM) can impede the removal of organic micro-pollutants (OMPs) through several mechanisms, including inner filter effect, competition with the...
Natural organic matter (NOM) can impede the removal of organic micro-pollutants (OMPs) through several mechanisms, including inner filter effect, competition with the target OMP, and radical scavenging, during synchronous adsorption/photocatalysis of multi-functional composites. In this study, the fate and inhibitory mechanisms of sulfamerazine (SMZ, a model OMP) that occurred in presence of seven different NOM samples (i.e. three standard NOM surrogates, a river water sample, a carbon filter effluent and two different sand filter effluents) during the adsorption/photocatalysis by a composite of BiO-TiO supported on powdered activated carbon (BiO-TiO/PAC, abbreviated as BTP) when exposed to visible light irradiation were revealed. The results indicated that adsorption played a greater attribution than photocatalysis on SMZ removal. The primary impediment to the adsorption and photocatalytic degradation of SMZ was attributed to the presence of terrestrial-derived, humic-like NOM fractions with high aromaticity. The adsorption efficacy of SMZ was weakened by the absorption of NOM and its degradation products onto the BTP surface. The inner filter effect, competition between NOM and SMZ, and radical scavenging were responsible for the reduced photocatalysis of SMZ. In the cases of real water matrices, the presence of inorganic anion and co-existed NOM reduced the removal of SMZ. In summary, the findings of this work offer a comprehensive comprehension of the impact of NOM fractions on photocatalysis, emphasizing the necessity to examine the interplay between NOM and background inorganic constituents in the degradation of OMP via adsorption/photocatalysis.
Topics: Adsorption; Water Pollutants, Chemical; Sulfamerazine; Humic Substances; Titanium; Bismuth; Catalysis; Water Purification; Light; Charcoal
PubMed: 37337954
DOI: 10.1080/09593330.2023.2224065