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Environmental Monitoring and Assessment Jul 2019In this study, the concentrations of nine typical antibiotics, including sulfadiazine (SD), sulfamerazine (SMR), sulfamethazine (SM2), sulfamethoxazole (SMZ), ofloxacin...
In this study, the concentrations of nine typical antibiotics, including sulfadiazine (SD), sulfamerazine (SMR), sulfamethazine (SM2), sulfamethoxazole (SMZ), ofloxacin (OFX), ciprofloxacin (CIP), trimethoprim (TMP), oxytetracycline (OTC), and tetracycline hydrochloride (TC), were detected in the Yitong River by solid-phase extraction high-performance liquid chromatography. The concentrations of the antibiotics were analyzed. Additionally, an improved immobilized substrate enzyme substrate method (DST-enzyme substrate method) was developed and used to evaluate the antibiotic resistance of coliform bacteria to OFX, CIP, enrofloxacin (ENR), TC, sulfisoxazole (SOX), and TMP in the Yitong River. The results showed that the concentrations of the nine antibiotics ranged from nd (not detected) to 1.361 μg/L. The detection rate and concentration of OFX were the highest, followed by CIP, and the detection rate and concentration of SM2 and OTC were the lowest. The detection rate and concentrations of antibiotics were higher in August and November than those in May. The antibiotics were mainly distributed in the livestock sewage discharge and suburban domestic sewage discharge areas. Moreover, the drug resistance of total coliform bacteria to fluoroquinolones, sulfonamides, tetracyclines, and TMP varied with season.
Topics: Anti-Bacterial Agents; China; Drug Resistance, Bacterial; Enterobacteriaceae; Environmental Monitoring; Rivers; Water Pollutants, Chemical
PubMed: 31350602
DOI: 10.1007/s10661-019-7660-4 -
The Science of the Total Environment Jan 2024Charge-assisted hydrogen bond (CAHB) is a key mechanism that affects the environmental behavior of pharmaceutical pollutants (PCs). However, the strength and stability...
Charge-assisted hydrogen bond (CAHB) is a key mechanism that affects the environmental behavior of pharmaceutical pollutants (PCs). However, the strength and stability of various CAHBs, and their effects on the co-sorption behavior of PCs are still unclear. Herein, DFT calculation with different solvent models including two implicit solvent model (PCM and SMD), and one explicit solvent model (ESM) were applied in this study, to investigate the effects of different CAHBs on the sorption and co-sorption behavior of four PCs (e.g., clofibric acid, p-aminobenzoic acid, acetaminophen, and sulfamerazine) on three model carbonaceous materials. First, the appearance of new peaks in the very low field of H NMR, and the blue shift of OH and NH peaks in FTIR indicated that CAHBs were indeed formed between PCs and carbonaceous materials. Next, according to the principal component analysis and correlation analysis of parameters (e.g., ΔE, bond length, bond angle, E, and ΔG) of these CAHBs calculated by the DFT with different solvent models, the results showed that SMD is the optimal model for calculating the strength and stability of CAHBs by DFT, and the strength and stability of CAHBs formed between PCs and carbonaceous materials in this study were in the order of homonuclear [O⋯H⋯O] CAHB > heteronuclear [O⋯HN]/[N⋯HO] type of CAHB > homonuclear [N⋯H⋯N]. Also, the co-sorption behavior of different PCs co-existing in binary systems further confirmed that, all above types of CAHBs formed between PCs and carbonaceous materials can produce obvious competition effect on the co-existing PCs that only OHB formed between them. This study not only reveals the environmental behavior of co-existing PCs, but also provides a theoretical basis for the design of obligate sorption materials for PCs in the natural environment.
PubMed: 37952672
DOI: 10.1016/j.scitotenv.2023.168375 -
Journal of Environmental Health Science... Dec 2021Antibiotic pollution in aquatic systems is a concern for human and environmental health. The concern is largely due to the global occurrence of antibiotic-resistant...
UNLABELLED
Antibiotic pollution in aquatic systems is a concern for human and environmental health. The concern is largely due to the global occurrence of antibiotic-resistant bacteria. From 2017 to 2018 in the NC Piedmont, 15 ion masses associated with antibiotics were detected in rural streams and groundwater. Four of these masses were confirmed to be antibiotics through target analysis (sulfamethoxazole, sulfamerazine, erythromycin, danofloxacin). Concentrations of antibiotics were as high as 1.8 μg/L. As a follow-up, antibiotic residues in urban streams sites in Greensboro, NC, USA, were investigated. Urban streams are heavily influenced by the dense populations surrounding them. In the fall, winter, and spring seasons, surface water was collected from eight sites along two urban streams. Sampling was conducted at streams sites above and below municipal hospitals and wastewater treatment facilities in the study area. At the conclusion of the survey, nine ion masses associated with antibiotics used in both human and veterinary medicine were detected from surface water collected. Three of the four antibiotics targeted in rural stream samples were detected and confirmed in urban stream samples (sulfamerazine, danofloxacin, and erythromycin). Detection frequencies of the three antibiotics ranged from 0 to 46%. Concentrations of each target antibiotic was as followed: SMX (0 to <10 ng/L), SMR (0 to <11 ng/L), DAN (0 to <20 ng/L), and ETM (0 to <15 ng/L). Each target antibiotic concentration was below our methods quantification limits. Our risk assessment analysis showed that the target antibiotics posed no risk to fish, daphnia, and green algae within this region of NC (RQ < 0.1). Compared to rural streams in this region of NC, antibiotic pollution is less prevalent in urban streams. The differences between urban and rural streams may be driven by the varying land use and suggest more research should be dedicated to monitoring these contaminants in rural areas of the United States.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s40201-021-00688-9.
PubMed: 34900268
DOI: 10.1007/s40201-021-00688-9 -
Journal of Colloid and Interface Science Oct 2022In the photo-Fenton reactions, fast recombination of photoinduced electrons and holes in Fe-based metal-organic frameworks (Fe-MOFs) slows Fe(III)/Fe(II) cycle, which...
In the photo-Fenton reactions, fast recombination of photoinduced electrons and holes in Fe-based metal-organic frameworks (Fe-MOFs) slows Fe(III)/Fe(II) cycle, which remains big challenge that significantly retards the overall process. Herein, NH-MIL-88B(Fe) (NM88) was modified with 3,5-diaminobenzoic acid (DB) and TPB-DMTP-COF (COF-OMe) to in situ construct NM88(DB)/COF-OMe composite that could strongly harvest the visible light for photo-Fenton degradation of sulfamerazine (SMR). With the addition of DB, electron-donating effect of NM88 was strengthened, which then promoted amino groups to react with aldehyde groups (Schiff-base), and thus highly facilitated the interfacial contact between NM88 and COF-OMe. Such modifications increased the degradation rate constants for NM88(DB)/COF-OMe to 15.1 and 17.3 times that of NM88 and COF-OMe respectively with good reusability. Moreover, the catalyst exhibited 32-170 times higher degradation kinetics in comparison to other reported catalysts. Results showed that due to the Schiff-base reaction between NM88(DB) and COF-OMe, electron density on Fe(III) was decreased; and the photogenerated electrons of COF-OMe moved to NM88(DB) to reduce Fe(III), thus resulting in the generation of highly active Fe(II) and ·OH species. Furthermore, the main reactive species were determined to be ·OH and ·O by trapping experiments, and a possible mechanism of the degradation system followed Z-scheme charge transfer.
Topics: Electronics; Ferric Compounds; Ferrous Compounds; Sulfamerazine
PubMed: 35660881
DOI: 10.1016/j.jcis.2022.05.142 -
Biochemical and Biophysical Research... Nov 2023The environmental and health risks associated with sulfonamide antibiotics (SAs) are receiving increasing attention. Through multi-spectroscopy, density functional...
The environmental and health risks associated with sulfonamide antibiotics (SAs) are receiving increasing attention. Through multi-spectroscopy, density functional theory (DFT), and molecular docking, this study investigated the interaction features and mechanisms between six representative SAs and human serum albumin (HSA). Multi-spectroscopy analysis showed that the six SAs had significant binding capabilities with HSA. The order of binding constants at 298 K was as follows: sulfadoxine (SDX): 7.18 × 10 L mol > sulfamethizole (SMT): 6.28 × 10 L mol > sulfamerazine (SMR): 2.70 × 10 L mol > sulfamonomethoxine (SMM): 2.54 × 10 L mol > sulfamethazine (SMZ): 3.06 × 10 L mol > sulfadimethoxine (SDM): 2.50 × 10 L mol. During the molecular docking process of the six SAs with HSA, the binding affinity range is from -7.4 kcal mol to -8.6 kcal mol. Notably, the docking result of HSA-SDX reached the maximum of -8.6 kcal mol, indicating that SDX may possess the highest binding capacity to HSA. HSA-SDX binding, identified as a static quenching and exothermic process, is primarily driven by hydrogen bonds (H bonds) or van der Waals (vdW) interactions. The quenching processes of SMR/SMZ/SMM/SDX/SMT to HSA are a combination of dynamic and static quenching, indicating an endothermic reaction. Hydrophobic interactions are primarily accountable for SMR/SMZ/SMM/SDX/SMT and HSA binding. Competition binding results revealed that the primary HSA-SAs binding sites are in the subdomain IB of the HAS structure, consistent with the results of molecule docking. The correlation analysis based on DFT calculations revealed an inherent relationship between the structural chemical features of SAs and the binding performance of HSA-SAs. The dual descriptor (DD) and the electrophilic Fukui function were found to have a significant relationship (0.71 and -0.71, respectively) with the binding constants of HSA-SAs, predicting the binding performance of SAs and HSA. These insights have substantial scientific value for evaluating the environmental risks of SAs as well as understanding their impact on biological life activities.
Topics: Humans; Serum Albumin, Human; Molecular Docking Simulation; Serum Albumin; Density Functional Theory; Sulfonamides; Protein Binding; Spectrometry, Fluorescence; Binding Sites; Anti-Bacterial Agents; Sulfanilamide; Circular Dichroism; Thermodynamics
PubMed: 37862782
DOI: 10.1016/j.bbrc.2023.10.040 -
Journal of Agricultural and Food... Dec 2021We have described a sulfonamide-selective ambient ion source coupled with electrospray ionization mass spectrometry (ESI-MS) for selective extraction and determination...
We have described a sulfonamide-selective ambient ion source coupled with electrospray ionization mass spectrometry (ESI-MS) for selective extraction and determination of trace sulfonamide antibiotics. It is obtained by modifying an iron sheet with a sulfadiazine-templated hydrophilic molecularly imprinted polymer (SF-HMIP). It behaves as both an online extractor and a MS ion source. Five sulfonamide antibiotics, including sulfamethoxazole (SMZ), sulfamerazine (SMR), sulfisoxazole (SIZ), sulfathiazole (ST), and sulfameter (SMD), were chosen to evaluate SF-HMIP coupled with ESI-MS, which showed good linearity in the range of 0.2-1000 ng/mL with correlation coefficient values () over 0.9946. The limits of detection (LODs) for analysis of pure water and honey were in the range of 0.1-0.2 and 0.2-1.5 ng/mL, respectively. Limits of quantitation (LOQs) for analysis of pure water and honey were in the range of 0.3-0.5 and 1.0-5.0 ng/mL, respectively. The results demonstrated that SF-HMIP combined with ESI-MS could be applied for the direct analysis of five trace sulfonamide compounds in honey and pure water with recoveries ranging from 76 to 129%.
Topics: Honey; Iron; Molecularly Imprinted Polymers; Solid Phase Extraction; Spectrometry, Mass, Electrospray Ionization; Sulfonamides
PubMed: 34898196
DOI: 10.1021/acs.jafc.1c06623 -
Journal of Hazardous Materials May 2020Animal manure containing veterinary antibiotics is a significant source of microbial antibiotic resistance genes (ARGs). Composting of animal manure with wheat straw and...
Animal manure containing veterinary antibiotics is a significant source of microbial antibiotic resistance genes (ARGs). Composting of animal manure with wheat straw and sawdust was explored as a means to reduce ARGs load in the final material. The effects of ciprofloxacin, oxytetracycline, sulfamerazine on the bacterial community composition, and how this then affected the removal of seven tetracycline resistance genes (TARGs), four sulfonamide resistance genes (SARGs), and two fluoroquinolone resistance genes (QARGs) were investigated. Treatments receiving either ciprofloxacin or the three mixed antibiotics had reduced bacterial alpha-diversity and displayed shifts in the abundance of Proteobacteria and Firmicutes. This demonstrated that different antibiotics played an important role in bacterial community composition. Furthermore, variation in the physicochemical properties of compost, particularly pH and temperature, was also strongly linked to shifts in bacterial composition over time. Based on network analysis, the reduction of TARGs were associated with loss of Pseudomonas, Pseudoxanthomonas, Pusillimonas, Aquamicrobium, Ureibacillus, Lysinibacillus, Bacillus and Brachybacterium during the thermophilic stage. However, QARGs and SARGs were more strongly affected by the presence of multiple antibiotics. Our results have important implications for reducing the spread of certain ARGs by controlling the composting temperature, pH or the antibiotics species used in husbandry.
Topics: Animals; Anti-Bacterial Agents; Ciprofloxacin; Composting; Drug Resistance, Bacterial; Genes, Bacterial; Manure; Microbiota; Oxytetracycline; Sulfamerazine; Swine
PubMed: 32004835
DOI: 10.1016/j.jhazmat.2020.122082 -
Scientific Reports May 2020Sulfadiazine (SD), sulfamerazine (SM1), and sulfamethazine (SM2) are widely used and disorderly discharged into surface water, causing contamination of lakes and rivers....
Sulfadiazine (SD), sulfamerazine (SM1), and sulfamethazine (SM2) are widely used and disorderly discharged into surface water, causing contamination of lakes and rivers. However, microalgae are regard as a potential resource to alleviate and degrade antibiotic pollution. The physiological changes of Chlorella vulgaris in the presence of three sulfonamides (SAs) with varying numbers of -CH groups and its SA-removal efficiency were investigated following a 7-day exposure experiment. Our results showed that the growth inhibitory effect of SD (7.9-22.6%), SM1 (7.2-45.9%), and SM2 (10.3-44%) resulted in increased proteins and decreased soluble sugars. Oxidative stress caused an increase in superoxide dismutase and glutathione reductase levels but decreased catalase level. The antioxidant responses were insufficient to cope-up with reactive oxygen species (hydrogen peroxide and superoxide anion) levels and prevent oxidative damage (malondialdehyde level). The ultrastructure and DNA of SA-treated algal cells were affected, as evident from the considerable changes in the cell wall, chloroplast, and mitochondrion, and DNA migration. C. vulgaris-mediated was able to remove up to 29% of SD, 16% of SM1, and 15% of SM2. Our results suggest that certain concentrations of specific antibiotics may induce algal growth, and algal-mediated biodegradation process can accelerate the removal of antibiotic contamination.
Topics: Anti-Bacterial Agents; Catalase; Chlorella vulgaris; Chlorophyll; Fresh Water; Glutathione Reductase; Malondialdehyde; Microalgae; Oxidative Stress; Photosynthesis; Reactive Oxygen Species; Sulfonamides; Superoxide Dismutase; Water Pollutants
PubMed: 32427937
DOI: 10.1038/s41598-020-65219-2 -
Journal of Hazardous Materials Feb 2022To improve cathodic HO accumulation and Fe reduction synchronously in the electro-Fenton (EF) process, a microbubble-assisted rotary tubular titanium cathode (MRTTC) was...
To improve cathodic HO accumulation and Fe reduction synchronously in the electro-Fenton (EF) process, a microbubble-assisted rotary tubular titanium cathode (MRTTC) was designed for the first time. By utilizing this MRTTC, HO accumulation improved by 4.05-fold, along with a 200% enhancement in iron reduction compared to the conventional EF process. This promotion is mainly attributed to a considerably higher oxygen mass transfer, which reduces the thickness of the adhered diffusion layer. The oxygen mass transfer coefficient (K) also improved from 0.0073 s to 0.012 s at a rotational speed of 300 rpm. In addition, the microbubble-assisted cathode further improved the K to 0.047 s. The synergistic effect between the rotating and microbubble-assisted cathodes further intensified HO accumulation in MRTTC. Apart from HO promotion, the iron reduction rate was elevated because the newly formed O provided an additional reduction pathway for Fe reduction in addition to the cathodic path. The effectiveness of MRTTC was confirmed by treating a benchmark organic pollutant, sulfamerazine (SMR), where approximately 100% SMR decay was obtained in 3 h. The results show that MRTTC is a novel and promising design in EF for antibiotic wastewater treatment.
Topics: Electrodes; Hydrogen Peroxide; Iron; Microbubbles; Oxidation-Reduction; Titanium; Water Pollutants, Chemical
PubMed: 34879586
DOI: 10.1016/j.jhazmat.2021.127403 -
Chemosphere Nov 2021Sulfonamide antibiotics (SAs) are used on a large scale in human and veterinary medicine. The main goal of this study was to develop a method for the detection of...
Sulfonamide antibiotics (SAs) are used on a large scale in human and veterinary medicine. The main goal of this study was to develop a method for the detection of selected SAs (sulfamethoxazole, sulfadiazine, sulfamethazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethiazole, and sulfisoxazole) in aqueous samples (targeted analysis), and then conduct a non-targeted analysis to determine the transformation products to elucidate their degradation pathways. These analyses were performed using ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. The procedure was used to detect selected antibiotics in water samples collected throughout a highly urbanized area. Among the studied compounds, sulfamethoxazole (max. 78.88 ng L) and sulfapyridine (max. 38.88 ng L ) were the most common pollutants identified in surface waters. Trace amounts of sulfadiazine (below LOQ = 0.40 ng L) were also detected. Next, the samples were screened to detect the transformation products. Several sulfadiazine and sulfamethoxazole transformation products were detected and confirmed in the environmental samples.
Topics: Anti-Bacterial Agents; Chromatography, High Pressure Liquid; Humans; Sulfonamides; Tandem Mass Spectrometry; Water; Water Pollutants, Chemical
PubMed: 34153907
DOI: 10.1016/j.chemosphere.2021.131189