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Frontiers in Physiology 2022Atrazine, one of the most intensively applied herbicides worldwide, is commonly found in several water bodies, affecting the associated fauna. Autochthon crustacean... (Review)
Review
Atrazine, one of the most intensively applied herbicides worldwide, is commonly found in several water bodies, affecting the associated fauna. Autochthon crustacean species have been relatively less studied, compared to vertebrate species, particularly concerning reproductive success. In this mini-review, we summarize the relevant information about the effects of atrazine exposure on the main reproductive aspects of crustaceans. One of these effects is related to the inhibition of ovarian growth. In this respect, a diminished vitellogenin content was found in the ovary of crabs exposed to atrazine during the entire period of ovarian growth, in correlation with a reduced oocyte size and a delay of ovarian maturation. Similar results were observed in crayfish. Atrazine was also able to affect the reproductive process, acting as an endocrine disruptor. In this sense, this herbicide was suspected to affect the secretion of some neurohormones involved in the gonadal growth, as well as to alter the circulating levels of steroid hormones which promote the synthesis of vitellogenin for ovarian growth. Moreover, atrazine induced sexual differentiation in juvenile crayfish toward a higher proportion of females, while it produced an increment of males in daphnids. Another aspect affected by this herbicide was the reduction of offspring production, as well as several embryonic abnormalities; genotoxic effects have been also reported in crayfish. Finally, some metabolic imbalances, such as reduction in energy reserves, have been observed in some species, together with oxidative stress and histopathological effects.
PubMed: 35784891
DOI: 10.3389/fphys.2022.926492 -
Reproduction, Fertility, and Development May 2021Vitellogenesis is essential for oocyte maturation. Vitellogenin (Vtg), a yolk precursor protein, plays an important role in oogenesis and vitellogenesis. Chinese hook...
Molecular cloning, characterisation and expression analysis of the vitellogenin genes vtgAo1 and vtgC during ovarian development in Chinese hook snout carp Opsariichthys bidens.
Vitellogenesis is essential for oocyte maturation. Vitellogenin (Vtg), a yolk precursor protein, plays an important role in oogenesis and vitellogenesis. Chinese hook snout carp Opsariichthys bidens is an economically important freshwater fish in China whose reproductive and developmental biology are not well understood. In this study, we undertook histological analysis to examine ovary development and oogenesis in O. bidens. The ovaries were divided into Stages II-V and oocytes were divided into perinuclear oocytes, cortical alveoli oocytes, vitellogenic oocytes and mature oocytes. Full-length cDNA sequences were cloned of two vtg genes from the liver of O. bidens, namely Ob-vtgAo1 and Ob-vtgC. Ob-vtgAo1 and Ob-vtgC cDNA are made up of 4136 and 4392 bases respectively and encode proteins containing 1335 and 1250 amino acids respectively. Ob-vtgAo1 contains three yolk protein domains: lipovitellin heavy chain (LvH), phosvitin (Pv) and lipovitellin light chain (LvL), whereas Ob-VtgC contains LvH and LvL, which are incomplete Vtgs. Ob-vtgAo1 and Ob-vtgC mRNA expression was significantly higher in the liver of O. bidens than in all other tissues. In oocytes of Stage II-III ovaries, yolk granules are almost absent and ovarian and hepatic Ob-vtgAo1 and Ob-vtgC expression is low. At Stage IV, the oocyte is filled with yolk granules and ovarian and hepatic Ob-vtgAo1 and Ob-vtgC expression is significantly increased. Collectively, these findings help us better understand vitellogenesis in O. bidens.
Topics: Animals; Carps; Cloning, Molecular; Female; Fish Proteins; Gene Expression Regulation, Developmental; Liver; Oocytes; Oogenesis; Ovary; Phylogeny; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Up-Regulation; Vitellogenesis; Vitellogenins
PubMed: 33858563
DOI: 10.1071/RD20294 -
International Archives of Allergy and... 2022Molecular studies of hen's egg allergens help define allergic phenotypes, with IgE to sequential (linear) epitopes on the ovomucoid (OVM) protein associated with a...
INTRODUCTION
Molecular studies of hen's egg allergens help define allergic phenotypes, with IgE to sequential (linear) epitopes on the ovomucoid (OVM) protein associated with a persistent disease. Epitope profiles of other egg allergens are largely unknown. The objective of this study was to construct an epitope library spanning across 7 allergens and further evaluate sequential epitope-specific (ses-)IgE and ses-IgG4 among baked-egg reactive or tolerant children.
METHODS
A Bead-Based Epitope Assay was used to identify informative IgE epitopes from 15-mer overlapping peptides covering the entire OVM and ovalbumin (OVA) proteins in 38 egg allergic children. An amalgamation of 12 B-cell epitope prediction tools was developed using experimentally identified epitopes. This ensemble was used to predict epitopes from ovotransferrin, lysozyme, serum albumin, vitellogenin-II fragment, and vitellogenin-1 precursor. Ses-IgE and ses-IgG4 repertoires of 135 egg allergic children (82 reactive to baked-egg, the remaining 52 tolerant), 46 atopic controls, and 11 healthy subjects were compared.
RESULTS
183 peptides from OVM and OVA were screened and used to create an aggregate algorithm, improving predictions of 12 individual tools. A final library of 65 sequential epitopes from 7 proteins was constructed. Egg allergic children had higher ses-IgE and lower ses-IgG4 to predominantly OVM epitopes than both atopic and healthy controls. Baked-egg reactive children had similar ses-IgG4 but greater ses-IgE than tolerant group. A combination of OVA-sIgE with ses-IgEs to OVM-023 and OVA-028 was the best predictor of reactive phenotype.
CONCLUSION
We have created a comprehensive epitope library and showed that ses-IgE is a potential biomarker of baked-egg reactivity.
Topics: Allergens; Animals; Chickens; Egg Hypersensitivity; Epitopes; Female; Humans; Immunoglobulin E; Immunoglobulin G; Ovalbumin; Ovomucin; Peptides; Vitellogenins
PubMed: 34818647
DOI: 10.1159/000519618 -
Current Biology : CB Jul 2019The roundworm C. elegans reversibly arrests larval development during starvation [1], but extended early-life starvation reduces reproductive success [2, 3]. Maternal...
The roundworm C. elegans reversibly arrests larval development during starvation [1], but extended early-life starvation reduces reproductive success [2, 3]. Maternal dietary restriction (DR) buffers progeny from starvation as young larvae, preserving reproductive success [4]. However, the developmental basis of reduced fertility following early-life starvation is unknown, and it is unclear how maternal diet modifies developmental physiology in progeny. We show here that extended starvation in first-stage (L1) larvae followed by unrestricted feeding results in a variety of developmental abnormalities in the reproductive system, including proliferative germ-cell tumors and uterine masses that express neuronal and epidermal cell fate markers. We found that maternal DR and reduced maternal insulin/insulin-like growth factor (IGF) signaling (IIS) increase oocyte provisioning of vitellogenin lipoprotein, reducing penetrance of starvation-induced abnormalities in progeny, including tumors. Furthermore, we show that maternal DR and reduced maternal IIS reduce IIS in progeny. daf-16/FoxO and skn-1/Nrf, transcriptional effectors of IIS, are required in progeny for maternal DR and increased vitellogenin provisioning to suppress starvation-induced abnormalities. daf-16/FoxO activity in somatic tissues is sufficient to suppress starvation-induced abnormalities, suggesting cell-nonautonomous regulation of reproductive system development. This work reveals that early-life starvation compromises reproductive development and that vitellogenin-mediated intergenerational insulin/IGF-to-insulin/IGF signaling mediates adaptation to nutrient availability.
Topics: Adaptation, Physiological; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Insulin; Larva; Nutrients; Signal Transduction; Somatomedins; Vitellogenins
PubMed: 31280992
DOI: 10.1016/j.cub.2019.05.062 -
Ecotoxicology and Environmental Safety Mar 2023The codling moth Cydia pomonella (Lepidoptera: Tortricidae) is a major invasive pest of pome fruits and walnuts worldwide. Lambda-cyhalothrin (LCT) and abamectin (AM)...
The codling moth Cydia pomonella (Lepidoptera: Tortricidae) is a major invasive pest of pome fruits and walnuts worldwide. Lambda-cyhalothrin (LCT) and abamectin (AM) have been frequently used in C. pomonella control, but control of this pest is very difficult because shortly after hatching, larvae of this insect bore tunnels and hide inside host plant fruit. In this study, a simulated field spray bioassay method was developed against neonate larvae of C. pomonella and concentration-response bioassays were conducted to evaluate the susceptibility of the neonate larvae to LCT and AM. Exposure of neonate larvae to sublethal concentration (LC) of LCT or AM significantly reduced the survival rate of larvae (4th and 5th instars), lowered the mean weight of larvae and pupae, and decreased the daily maximal number of eggs laid and the total number of eggs laid (fecundity) per female. The sublethal effects, including reduced body mass, mean fecundity and net reproductive rate, extended mean generation time, and shortened oviposition period, were also found in transgenerational offspring. Furthermore, the transgenerational maternal effects were more obvious for AM than LCT, in comparison to the control. Additionally, the estimated population size was decreased by exposure to LC of LCT and AM, and the observed reduction of fecundity and population size within and across generations was likely the result of the downregulation of the reproduction-related vitellogenin gene (CpVg) after exposure to LC of LCT and AM. These results provide a better understanding of the overall effects of LCT and AM on C. pomonella and the transgenerational effects which should be taken into consideration when using insecticides in order to control C. pomonella.
Topics: Animals; Female; Moths; Pyrethrins; Larva; Insecticides; Reproduction
PubMed: 36731179
DOI: 10.1016/j.ecoenv.2023.114581 -
Aquatic Toxicology (Amsterdam,... Jun 2022Octyl methoxycinnamate (OMC) is a common UV filter found in personal care products such as sunscreen and cosmetics. However, OMC's presence in wastewater has raised...
Octyl methoxycinnamate (OMC) is a common UV filter found in personal care products such as sunscreen and cosmetics. However, OMC's presence in wastewater has raised concerns that it could potentially pollute aquatic ecosystems because of its limited biodegradability and its estrogenic disrupting properties. In this study, we investigated the environmental toxicity of OMC and its potential biomarkers using the nematode Caenorhabditis elegans. Our results showed that body length, eggs in utero, and total brood size decreased with increasing dose (experimental concentrations = 0, 1, 5, 10, 100, 500 μM for body length and eggs in utero, and 0, 5, 10 μM for total brood size) in C. elegans after L1 larval stage (the first larval stage for 0 - 12 hours post-hatching) larval stage exposure to OMC. The minimum effective concentrations were 1, 5, and 10 μM, respectively. Modeling results demonstrated that the threshold concentration of OMC inducing 10% inhibited eggs in utero was 0.33 μM (95.11 μg/L). Furthermore, germline apoptosis was induced in 10 μM OMC-treated worms (experimental concentrations = 0, 5, 10 μM). Decreased mRNA levels of vitellogenin-related genes (vit-2 and vit-6) and increased mRNA levels of apoptosis-related genes (egl-1 and ced-3) were observed in 10 μM OMC-treated C. elegans (experimental concentrations = 0, 10 μM), suggesting that reproductive toxicity was associated with decreased vitellogenin levels and germline apoptosis. In summary, our study shows that OMC is reproductively toxic and leads to reduced egg formation and decreased brood size in C. elegans by reducing vitellogenin levels and promoting germline apoptosis.
Topics: Animals; Apoptosis; Caenorhabditis elegans; Ecosystem; Germ Cells; RNA, Messenger; Vitellogenins; Water Pollutants, Chemical
PubMed: 35397382
DOI: 10.1016/j.aquatox.2022.106149 -
Comparative Biochemistry and... May 2022Adult zebrafish (Danio rerio) were electrolyzed at different current densities to explore the effects of electrolysis on their reproductive system, especially on embryo...
Adult zebrafish (Danio rerio) were electrolyzed at different current densities to explore the effects of electrolysis on their reproductive system, especially on embryo production, and to uncover the molecular mechanism of changes in sex hormone and vitellogenin (VTG) levels. The results showed that embryo reproduction of zebrafish was reduced at a current density of 0.64 A/m after 28 days of exposure. In addition, the 17β-estradiol concentration significantly decreased and the testosterone concentration increased in female zebrafish above 0.53 A/m. However, opposite trends were observed in male zebrafish. The VTG concentration was reduced considerably in the livers of female zebrafish in the 0.64 A/m electrolysis group (p < 0.05). In addition, the mRNA expression of hormone-regulating genes was significantly altered in female and male zebrafish when the current density was greater than 0.53 A/m, and their change trends were sex-dependent. The genes expression levels of vtg1 and esr1 were downregulated in female zebrafish. However, the gene expression of esr1 and cyp19a was upregulated in male zebrafish. These changes were related to disruption in the hormone balance and VTG levels of adult zebrafish. Thus, electrolysis could cause masculinization of female zebrafish and feminization of male zebrafish. Nonetheless, there were few influences on the hormone levels and reproduction rate of adult zebrafish at the threshold of 0.26 A/m. Thus, the current density of electrolysis needs to be controlled within a specific range to reduce its harmful effects on the reproductive system of aquatic animals.
Topics: Animals; Electrolysis; Endocrine Disruptors; Female; Gonads; Male; Reproduction; Sex Factors; Vitellogenins; Zebrafish
PubMed: 35131430
DOI: 10.1016/j.cbpc.2022.109294 -
Frontiers in Endocrinology 2021This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of female crabs, as well as the... (Review)
Review
This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of female crabs, as well as the effects of glyphosate on sperm production in males of the same species. After long-term assays, made during the 3-month pre-reproductive period of this species, both formulated and technical glyphosate were able to produce a significant incidence of oocyte reabsorption in the ovary, together with a concomitant decreased of vitellogenin content, at concentrations ranging from 0.2 to 1 mg/L. Despite this, after 32-day assays, glyphosate stimulated oocyte growth, in terms of a higher percentage of vitellogenic oocytes, suggesting that glyphosate could be acting as an endocrine disruptor. assays made with isolated ovarian pieces showed a decrease of vitellogenin content, in correlation with lower protein synthesis, although some advance in maturation was observed in the histological analysis. In male crabs exposed to both technical and formulated glyphosate at 1 mg/L, several reproductive imbalances were noted, such as a significant decrease of the sperm count, abnormal spermatophores, and possible disrupting effects of glyphosate on the androgenic gland.
Topics: Androgens; Animals; Brachyura; Dose-Response Relationship, Drug; Endocrine Disruptors; Female; Glycine; Herbicides; Male; Models, Animal; Oocytes; Ovary; Reproduction; Spermatozoa; Vitellogenins; Water Pollutants, Chemical; Glyphosate
PubMed: 33841335
DOI: 10.3389/fendo.2021.643168 -
Chemosphere May 2020Parabens, the alkyl esters of p-hydroxybenzoic acid such as methylparaben (MeP), ethylparaben (EtP), propylparaben (PrP), butylparaben (BuP) are used as a preservative...
Parabens, the alkyl esters of p-hydroxybenzoic acid such as methylparaben (MeP), ethylparaben (EtP), propylparaben (PrP), butylparaben (BuP) are used as a preservative in food, personal care products (PCPs), and pharmaceuticals, due to their antimicrobial properties. Parabens are continuously released into the environment, during washout of PCPs, disposal of industrial waste from the pharmaceutical and paper industries. Parabens have been detected in the indoor dust, wastewater stream, surface water of rivers, and the marine system. Recent eco-toxicological data and the environmental presence of parabens, has raised concerns regarding the safety and health of environment/humans. Thus, to further understand the toxicity of parabens, the present study was carried out in the soil nematode and well established biological model organism Caenorhabditis elegans. In the present study, LC of MeP, EtP, PrP and BuP for 72 h exposures from L1 larva to adult stage was found to be 278.1, 217.8, 169.2, and 131.88 μg/ml, respectively. Further exposure to 1/5th of LC of parabens yielded an internal concentration ranging from 1.67 to 2.83 μg/g dry weight of the organism. The toxicity of parabens on the survival, growth, behavior, and reproduction of the C. elegans was found in the order of BuP > PrP > EtP > MeP. Worms exposed to parabens show significant down-regulation of vitellogenin genes, high levels of reactive oxygen species and anti-oxidant transcripts, the latter being concordant with nuclear localization of DAF-16 and up-regulation of HSF-1 and SKN-1/Nrf. Hence, parabens caused endocrine disruption, oxidative stress and toxicity in C. elegans at environment relevant internal concentration of parabens.
Topics: Animals; Caenorhabditis elegans; Cosmetics; Endocrine Disruptors; Environmental Pollutants; Humans; Lethal Dose 50; Parabens; Preservatives, Pharmaceutical; Rivers; Toxicity Tests; Vitellogenins
PubMed: 31927363
DOI: 10.1016/j.chemosphere.2019.125730 -
Ecotoxicology and Environmental Safety Nov 2023Vitellogenin (VTG), a biomarker for endocrine activity, is a mechanistic component of the regulatory assessment of potential endocrine-disrupting properties of... (Review)
Review
Vitellogenin (VTG), a biomarker for endocrine activity, is a mechanistic component of the regulatory assessment of potential endocrine-disrupting properties of chemicals. This review of VTG data is based on changes reported for 106 substances in standard fish species. High intra-study and inter-laboratory variability in VTG concentrations was confirmed, as well as discrepancies in interpretation of results based on large differences between fish in the dilution water versus solvent control, or due to the presence of outlier measurements. VTG responses in fish were ranked against predictions for estrogen receptor agonist activity and aromatase inhibition from bioactivity model output and ToxCast in vitro assay results, respectively. These endocrine mechanisms explained most of the VTG responses in the absence of systemic toxicity, the magnitude of the VTG response being proportional to the in vitro potency. Interpretation of the VTG data was sometimes confounded by an alternative endocrine mechanism of action. There was evidence for both false positive and negative responses for VTG synthesis, but overall, it was rare for substances without endocrine activity in vitro to cause a concentration-dependent VTG response in fish in the absence of systemic toxicity. To increase confidence in the VTG results, we recommend improvements in the VTG measurement methodologies and greater transparency in reporting of VTG data (including quality control criteria for assay performance). This review supports the application of New Approach Methodologies (NAMs) by demonstrating that endocrine activity in vitro from mammalian cell lines is predictive for in vivo VTG response in fish, suggesting that in vitro mechanistic data could be used more broadly in decision-making to help reduce animal testing.
Topics: Animals; Vitellogenins; Fishes; Estrogens; Endocrine Disruptors; Water Pollutants, Chemical; Mammals
PubMed: 37827093
DOI: 10.1016/j.ecoenv.2023.115563