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Microorganisms Jun 2019is an ocular bacterial pathogen isolated in cases of keratitis, conjunctivitis, and endophthalmitis. Gram-negative brick-shaped diplobacilli from ocular specimens, and...
is an ocular bacterial pathogen isolated in cases of keratitis, conjunctivitis, and endophthalmitis. Gram-negative brick-shaped diplobacilli from ocular specimens, and slow growth in culture, are early indications of ocular infection; however, identifying to species can be complex and inconsistent. In this study, bacteria consistent with were identified to species using: (1) DNA sequencing coupled with vancomycin susceptibility, (2) MALDI-TOF mass spectrometry, and (3) the Biolog ID system. Study samples consisted of nine ATCC controls, 82 isolates from keratitis, 21 isolates from conjunctivitis, and 4 isolates from endophthalmitis. The ATCC controls were correctly identified. For keratitis, 66 (80.5%) were identified as , 7 (9.0%) as , 5 (6%) as , 2 (2.5%) as , 1 (1.0%) as , and 1 (1.0%) as . For conjunctivitis, 9 (43.0%) were identified as , 6 (29.0%) as , 3 (14.3%) as , 2 (9.5%) as (), and 1 (4.5%) as . From endophthalmitis, 3 of 4 of the isolates were . Overall, . and were identified in 70% (75 of 107) and 13% (14 of 107) of cases, respectively, totaling 83% (89 of 107). and are important bacterial pathogens of the eye as determined by DNA sequencing, MALDI-TOF MS, and Biolog. Although is a clinical pathogen, other species of appear to have a prominent role in eye infections.
PubMed: 31167433
DOI: 10.3390/microorganisms7060163