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International Journal of Molecular... Nov 2023() is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in...
() is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resistant strains and the decline in novel antibiotic candidates have necessitated the development of alternative antimicrobial agents. In this study, AVPL, the () phage lysin, was found to exhibit efficient bactericidal activity and broad lytic activity against multiple serotypes of . A final concentration of 300 μg/mL AVPL reduced counts by 4-4.5 log10 within 1 h in vitro. Importantly, AVPL effectively inhibited 48 h biofilm formation and disrupted preformed biofilms. In a mouse model, 300 μg/mouse AVPL protected 100% of mice from infection following the administration of lethal doses of multidrug-resistant type 2 (SS2) strain SC19, reduced the bacterial load in different organs, and effectively alleviated inflammation and histopathological damage in infected mice. These data suggest that AVPL is a valuable candidate antimicrobial agent for treating infections.
Topics: Animals; Swine; Humans; Mice; Bacteriophages; Aerococcus; Streptococcus suis; Streptococcal Infections; Bacteremia; Disease Models, Animal
PubMed: 38068990
DOI: 10.3390/ijms242316670 -
European Journal of Pediatrics Feb 2023Aerococcus urinae (A. urinae) is primarily recognized as a common pathogen in the geriatric population, causing urinary tract infection (UTI), sepsis, and endocarditis,...
Aerococcus urinae (A. urinae) is primarily recognized as a common pathogen in the geriatric population, causing urinary tract infection (UTI), sepsis, and endocarditis, predominantly in female patients. In the paediatric population, only a few case reports exist suggesting A. urinae causes malodorous urine in otherwise healthy boys. In this study, we investigated the spectrum of clinical and laboratory presentations of A. urinae detection in children. A retrospective, single-centre, case series including all patients with the detection of A. urinae during a 7-year study period. Patients with detection of A. urinae only in non-urogenital skin swabs were excluded. A total of 40 samples from 33 patients were identified of which 20 patients were included in the final analysis. The median (IQR) age was 6.8 (2.9-9.5) years; 18 (90%) patients were boys. Four patients were diagnosed with a UTI, six had malodorous urine without UTI, three were diagnosed with balanitis and seven showed A. urinae colonization in the urine culture. Urogenital disorders were present in 12 patients. Additional pathogens were detected in 13 patients. Recurrence of detection during our study period was observed in four (20%) patients. Conclusion: Beyond malodorous urine, A. urinae detection is associated with more severe presentations including UTI in the paediatric population. Pre-existing urogenital disorders were frequent, and therefore, a nephro-urological investigation should be considered in all cases of A. urinae detection in the paediatric population. What is Known: • Aerococcus urinae (A. urinae) is known to be a common pathogen in the geriatric population, causing urinary tract infection (UTI), sepsis, and endocarditis, predominantly in female patients. • In the paediatric population, A. urinae is mainly described as a low-grade pathogen. Some case reports describe A. urinae as the cause of extraordinary malodorous urine in otherwise healthy boys. What is New: • Beyond malodorous urine, A. urinae detection is associated with more severe presentations including UTI in the paediatric population. • A. urinae was mainly detected in boys with pre-existing urogenital disorders; therefore, a nephro-urological investigation should be considered in cases of A. urinae detection in the paediatric population.
Topics: Aged; Male; Humans; Child; Female; Retrospective Studies; Anti-Bacterial Agents; Microbial Sensitivity Tests; Urinary Tract Infections; Aerococcus; Sepsis; Endocarditis; Urinary Tract; Gram-Positive Bacterial Infections
PubMed: 36472648
DOI: 10.1007/s00431-022-04730-2 -
Medicina (Kaunas, Lithuania) Oct 2019The objective of this study was to investigate the clinical significance of isolates from blood stream infection known to be blood culture contaminants in pediatric...
The objective of this study was to investigate the clinical significance of isolates from blood stream infection known to be blood culture contaminants in pediatric patients. Microbiological reports and medical records of all blood culture tests issued from 2002 to 2012 ( = 76,331) were retrospectively reviewed. Evaluation for potential contaminants were done by reviewing medical records of patients with the following isolates: coagulase-negative Staphylococcus, viridans group Streptococcus, , and species. Repeated cultures with same isolates were considered as a single case. Cases were evaluated for their status as a pathogen. Coagulase-negative Staphylococcus had clinical significance in 23.8% of all cases. Its rate of being a true pathogen was particularly high in patients with malignancy (43.7%). Viridans group Streptococcus showed clinical significance in 46.2% of all cases. Its rate of being a true pathogen was similar regardless of the underlying morbidity of the patient. The rate of being a true pathogens for remaining isolates was 27.7% for and 19.0% for species. Coagulase-negative Staphylococcus and viridans group Streptococcus isolates showed high probability of being true pathogens in the pediatric population, especially in patients with underlying malignancy.
Topics: Aerococcus; Bacillus; Bacteremia; Blood Culture; Child, Preschool; Corynebacterium; Female; Humans; Infant; Infant, Newborn; Male; Micrococcus; Pediatrics; Retrospective Studies; Staphylococcus; Viridans Streptococci
PubMed: 31627324
DOI: 10.3390/medicina55100696 -
TheScientificWorldJournal 2021Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is...
Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolating, and identifying lactic acid bacteria and evaluating their capacity to produce biomolecules with antibacterial activity. Among 44 strains identified, 43 are Gram-positive, and most are catalase-negative and cocci-shaped. Five strains were selected to evaluate their antibacterial activity against , and . Different induction methods allowed to amplify the antibacterial effects against these pathogenic strains.
Topics: Aerococcus; Animals; Anti-Bacterial Agents; Culture Media, Conditioned; Dairying; Enterococcus; Enterococcus faecalis; Equidae; Escherichia coli; Female; Food Microbiology; Lactation; Leuconostoc mesenteroides; Listeria monocytogenes; Microbial Sensitivity Tests; Milk; Morocco; Staphylococcus aureus
PubMed: 34421400
DOI: 10.1155/2021/6664636 -
The Journal of Applied Laboratory... Jan 2020
Topics: Abiotrophia; Cerebrospinal Fluid Leak; Child; Craniotomy; Diagnosis, Differential; Electrophoresis; Humans; Infections; Neuraminidase; Postoperative Complications; Transferrin
PubMed: 31811077
DOI: 10.1373/jalm.2019.031096 -
Journal of Clinical Microbiology Sep 2021
Topics: Abiotrophia; Discitis; Endocarditis; Endocarditis, Bacterial; Gram-Positive Bacterial Infections; Humans
PubMed: 34542326
DOI: 10.1128/JCM.03093-20 -
Anales Del Sistema Sanitario de Navarra Dec 2020Abiotrophia defectiva is a rare cause of endocarditis with a high embolization rate compared to other streptococcal endocarditis. Neurological complications may occur in...
Abiotrophia defectiva is a rare cause of endocarditis with a high embolization rate compared to other streptococcal endocarditis. Neurological complications may occur in 20-40% of cases, and include intracranial aneurysms and sub-arachnoid hemorrhage. Its isolation in the central nervous system (CNS) is extremely rare and is associated with previous neurosurgical procedures. We present the case of a 51-year-old patient who suffered an ischemic stroke as a complication of A. defectiva endocarditis, and the cases of CNS infections caused by this microorganism are reviewed. The relevance of the case lies in its singular character, due to the absence of previous valve disease in the patient without a recent history of invasive procedures or surgical interventions.
Topics: Abiotrophia; Embolic Stroke; Endocarditis, Bacterial; Gram-Positive Bacterial Infections; Humans; Middle Aged
PubMed: 32814921
DOI: 10.23938/ASSN.0881 -
Journal of Bacteriology Jun 2020is increasingly recognized as a potentially significant urinary tract bacterium. has been isolated from urine collected from both males and females with a wide range...
is increasingly recognized as a potentially significant urinary tract bacterium. has been isolated from urine collected from both males and females with a wide range of clinical conditions, including urinary tract infection (UTI), urgency urinary incontinence (UUI), and overactive bladder (OAB). is of particular clinical concern because it is highly resistant to many antibiotics and, when undiagnosed, can cause invasive and life-threatening bacteremia, sepsis, or soft tissue infections. Previous genomic characterization studies have examined strains isolated from patients experiencing UTI episodes. Here, we analyzed the genomes of strains isolated as part of the urinary microbiome from patients with UUI or OAB. Furthermore, we report that certain strains exhibit aggregative phenotypes, including flocking, which can be modified by various growth medium conditions. Finally, we performed in-depth genomic comparisons to identify pathways that distinguish flocking and nonflocking strains. is a urinary bacterium of emerging clinical interest. Here, we explored the ability of 24 strains of isolated from women with lower urinary tract symptoms to display aggregation phenotypes We sequenced and analyzed the genomes of these strains. We performed functional genomic analyses to determine whether the hyperflocking aggregation phenotype displayed by certain strains was related to the presence or absence of certain pathways. Our findings demonstrate that strains have different propensities to display aggregative properties and suggest a potential association between phylogeny and flocking.
Topics: Aerococcus; Anti-Bacterial Agents; Biofilms; Female; Genome, Bacterial; Gram-Positive Bacterial Infections; Humans; Lower Urinary Tract Symptoms; Male; Microbial Sensitivity Tests; Phylogeny
PubMed: 32284319
DOI: 10.1128/JB.00170-20 -
Internal Medicine (Tokyo, Japan) Nov 2022To our knowledge, there have been no reports of right-sided infective endocarditis (RSIE) with ventricular free wall vegetation caused by Abiotrophia defectiva. We...
To our knowledge, there have been no reports of right-sided infective endocarditis (RSIE) with ventricular free wall vegetation caused by Abiotrophia defectiva. We herein report a case of RSIE caused by A. defectiva with ventricular free wall vegetation in a 27-year-old man with ventricular septal defect (VSD). Computed tomography showed multiple bilateral pulmonary nodular shadows. Transesophageal echocardiography (TEE) demonstrated right ventricular free wall vegetation at the jet stream. Blood culture revealed A. defectiva. These findings are consistent with a diagnosis of infective endocarditis and septic pulmonary embolism. Treatment with ceftriaxone and gentamicin and subsequent surgical VSD closure improved the patient's condition without recurrence.
Topics: Male; Humans; Adult; Abiotrophia; Endocarditis, Bacterial; Heart Septal Defects, Ventricular; Heart Ventricles; Endocarditis
PubMed: 35431307
DOI: 10.2169/internalmedicine.9374-22 -
Journal of General Internal Medicine Feb 2023
Topics: Humans; Abiotrophia; Antibodies, Antineutrophil Cytoplasmic; Endocarditis; Glomerulonephritis; Vasculitis
PubMed: 36460879
DOI: 10.1007/s11606-022-07960-0