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MicrobiologyOpen Dec 2019Pivmecillinam (amdinocillin pivoxil) is the recommended first-choice antibiotic used to treat urinary tract infections (UTIs) in Denmark. The frequency of mutation to...
Pivmecillinam (amdinocillin pivoxil) is the recommended first-choice antibiotic used to treat urinary tract infections (UTIs) in Denmark. The frequency of mutation to mecillinam (MEC) resistance is described as high in vitro; however, treatment of UTI has a good clinical response and prevalence of mecillinam resistance in Escherichia coli remains low despite many years of use. We describe occurrence of in vivo mecillinam resistance in a clinical isolate of ESBL-producing E. coli following pivmecillinam treatment. The identified phenotypic differences in the mecillinam resistant isolate compared with the original mecillinam susceptible isolate were a full-length LPS with O-antigen (O25), mecillinam resistance and a lower MIC for ceftazidime. Regarding genotype, the resistant isolate differed with a mutation in bla to bla , loss of a part of a plasmid and a genomic island, respectively, and insertion of a transposase in wbbL, causing the rough phenotype. The observed mecillinam resistance is expected to be caused by the mutation in bla with additional contribute from the serotype shift. We continue to recommend the use of pivmecillinam as first-line treatment for UTI.
Topics: Amdinocillin; Amdinocillin Pivoxil; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Infections; Genome, Bacterial; Genomics; Humans; Microbial Sensitivity Tests; Multilocus Sequence Typing; Mutation; Phylogeny; Whole Genome Sequencing; beta-Lactamases
PubMed: 31573735
DOI: 10.1002/mbo3.941 -
Scandinavian Journal of Primary Health... Mar 2020
Topics: Adolescent; Adult; Amdinocillin Pivoxil; Anti-Bacterial Agents; Denmark; Escherichia coli; Humans; Male; Middle Aged; Nitrofurantoin; Prescriptions; Retrospective Studies; Trimethoprim; Urinary Tract Infections; Young Adult
PubMed: 31997687
DOI: 10.1080/02813432.2020.1718301