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Acta Odontologica Latinoamericana : AOL Dec 2022Dentin hypersensitivity (DH) is characterized by rapid, acute pain arising from exposed dental tubules.
UNLABELLED
Dentin hypersensitivity (DH) is characterized by rapid, acute pain arising from exposed dental tubules.
AIM
the aim of this study was to evaluate the roughness, tubule occlusion, and permeability of eroded dentin brushed with different toothpastes.
MATERIALS AND METHOD
ninety bovine teeth were cut into blocks. Thirty hemifaces were protected with varnish and the other sixty were submitted to permeability tests. Specimens were divided into groups according to the dentifrices: without fluoride (WF), sodium fluoride (NaF), and stannous fluoride (SnF2). The blocks were subjected to a 5-day erosive-abrasive protocol. Surface roughness and dentinal tubule occlusion (n=10) were assessed for both control and test hemifaces of the same sample along with permeability analysis (n=20). Two-way RM ANOVA and Tukey's post-hoc test were performed (p≤0.05).
RESULTS
NaF and SnF2 presented higher roughness than WF. The number of open tubules was higher in WF. Permeability was higher in SnF2, but there was no significant difference between WF and NaF.
CONCLUSIONS
both fluoride toothpastes occluded dentinal tubules and increased roughness. NaF toothpaste promoted greater decrease in dentin permeability.
Topics: Animals; Cattle; Toothpastes; Fluorides; Dentin; Dentin Sensitivity; Sodium Fluoride; Permeability; Dentin Permeability
PubMed: 36748742
DOI: 10.54589/aol.35/3/229 -
Scientific Reports Jul 2021This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay...
This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental pulp stem cells (HDPSCs) and gingival fibroblast cells (HGFCs) following either indirect exposure to 3 commercially available concentrations of CP gel using an in-vitro dentin perfusion assay or direct exposure to 5% HO were investigated by evaluating change in cell morphology and by hemocytometry. The 5% and 16% CP produced a significantly lower (p < 0.001) enamel protein content (by weight) when compared to the control. The organic content in enamel varied accordingly to the CP treatment: for the 16% and 5% CP treatment groups, a variation of 4.0% and 5.4%, respectively, was observed with no significant difference. The cell viability of HDPSCs decreased exponentially over time for all groups. Within the limitation of this in-vitro study, we conclude that even low concentrations of HO and CP result in a deleterious change in enamel protein content and compromise the viability of HGFCs and HDPSCs. These effects should be observed in-vivo.
Topics: Bicuspid; Carbamide Peroxide; Cell Survival; Cells, Cultured; Dental Enamel; Dental Pulp; Dentin; Humans; Hydrogen Peroxide; Molar; Tooth Bleaching Agents
PubMed: 34330953
DOI: 10.1038/s41598-021-94745-w -
Journal of Endodontics Nov 2019Odontoblasts produce dentin throughout life and in response to trauma. The purpose of this study was to identify the roles of endogenous Wnt signaling in regulating the...
INTRODUCTION
Odontoblasts produce dentin throughout life and in response to trauma. The purpose of this study was to identify the roles of endogenous Wnt signaling in regulating the rate of dentin accumulation.
METHODS
Histology, immunohistochemistry, vital dye labeling, and histomorphometric assays were used to quantify the rate of dentin accumulation as a function of age. Two strains of Wnt reporter mice were used to identify and follow the distribution and number of Wnt-responsive odontoblasts as a function of age. To show a causal relationship between dentin secretion and Wnt signaling, dentin accumulation was monitored in a strain of mice in which Wnt signaling was aberrantly elevated.
RESULTS
Dentin deposition occurs throughout life, but the rate of accumulation slows with age. This decline in dentin secretion correlates with a decrease in endogenous Wnt signaling. In a genetically modified strain of mice, instead of tubular dentin, aberrantly elevated Wnt signaling resulted in accumulation of reparative dentin or osteodentin secreted from predontoblasts.
CONCLUSIONS
Wnt signaling regulates dentin secretion by odontoblasts, and the formation of reparative or osteodentin is the direct consequence of elevated Wnt signaling. These preclinical data have therapeutic implications for the development of a biologically based pulp capping medicant.
Topics: Animals; Dental Pulp; Dentin; Mice; Odontoblasts; Odontogenesis; Wnt Signaling Pathway; beta Catenin
PubMed: 31522810
DOI: 10.1016/j.joen.2019.07.014 -
Frontiers in Physiology 2020Dentin dysplasia (DD) and dentinogenesis imperfecta (DGI) patients have abnormal structure, morphology, and function of dentin. DD-II, DGI-II, and DGI-III are caused by...
Dentin dysplasia (DD) and dentinogenesis imperfecta (DGI) patients have abnormal structure, morphology, and function of dentin. DD-II, DGI-II, and DGI-III are caused by heterozygous mutations in the dentin sialophosphoprotein () gene in humans. Evidences have shown that loss of function of DSPP in knockout mice leads to phenotypes similar to DGI-III, and that the abnormal dentinogenesis is associated with decreased levels of DSPP, indicating that DSPP haploinsufficiency may play a role in dentinogenesis. Thus, to testify the haploinsufficiency of , we used a heterozygous mouse model to observe the phenotypes in the teeth and the surrounding tissues. We found that heterozygous mice displayed dentin phenotypes similar to DD-II at the ages of 12 and 18 months, which was characterized by excessive attrition of the enamel at the occlusal surfaces, thicker floor dentin of the pulp chamber, decreased pulp volume, and compromised mineralization of the dentin. In addition, the periodontium was also affected, exhibiting apical proliferation of the junctional epithelium, decreased height and width of the alveolar bone, and infiltration of the inflammatory cells, leading to the destruction of the periodontium. Both the dental and periodontal phenotypes were age-dependent, which were more severe at 18 months old than those at 12 months old. Our report is the first to claim the haploinsufficiency of gene and a DD-II mouse model, which can be further used to study the molecular mechanisms of DD-II.
PubMed: 33240110
DOI: 10.3389/fphys.2020.593626 -
Zhejiang Da Xue Xue Bao. Yi Xue Ban =... Apr 2021To investigate the effect of captopril on the dentin bonding durability of self-etch adhesive. Different concentrations of captopril ethanol solutions or captopril...
To investigate the effect of captopril on the dentin bonding durability of self-etch adhesive. Different concentrations of captopril ethanol solutions or captopril ethanol/water solutions were prepared to pretreat dentin as primer for the self-etch adhesives. The surface morphology of the dentin was observed with scanning electron microscopy (SEM). Based on the morphology analysis, the pretreatment condition was selected and two self-etch adhesives were employed to evaluate the improvement effect of the captopril pretreatment on the dentin bonding durability. : SEM showed that the pretreatment of captopril ethanol solutions and captopril ethanol/water solutions were able to remove the smear lay and partially expose collagen matrix. According to the SEM results, the pretreating condition of captopril ethanol/water solution with the pretreating time of was selected for further dentin bonding study. For Clearfil SEBOND system, the immediate bonding strength increased from to (<0.05). After one-year aging, the bonding strength of the control group decreased markedly [(22.90±6.82) MPa, <0.05]; while the bonding strength of the captopril pretreated group kept steadily >0.05]. For Clearfil S BOND system, there was no significant difference in the immediate bonding strength between the experimental group [(4.07) MPa] and the control group[(4.11) MPa]. But after one-year aging, the bonding strength of the experimental group was higher than that of the control group <0.05]. : The pretreatment with captopril ethanol/water solution increases the dentin bonding strength of the self-etch adhesive systems and also improves the bonding durability.
Topics: Adhesives; Captopril; Dental Bonding; Dentin; Dentin-Bonding Agents; Materials Testing; Microscopy, Electron, Scanning; Resin Cements
PubMed: 34137232
DOI: 10.3724/zdxbyxb-2021-0116 -
Annals of Translational Medicine Mar 2021The purpose of this study was to investigate the effect of a complex of polyamide-amine dendrimer (PAMAM) and chlorhexidine gluconate (CG) on remineralization of dentin...
BACKGROUND
The purpose of this study was to investigate the effect of a complex of polyamide-amine dendrimer (PAMAM) and chlorhexidine gluconate (CG) on remineralization of dentin in an artificial simulated resin dentin bonding microenvironment.
METHODS
The structure of this complex was characterized by FT-IR. Twelve standard dentin samples were randomly divided into four treatment fluid groups namely a PAMAM group, CG group, PAMAM + CG group, and deionized water group. A microenvironmental mineralization model was established with 50 µm gap width between resin and dentin. The dentin surface was observed by a scanning electron microscope (SEM), and the chemical structure of the surface was analyzed by X-ray energy spectrum (EDS), X-ray diffraction (XRD), and laser Raman spectroscopy.
RESULTS
SEM showed the density of dentinal tubules exposed in the PAMAM group decreased after a 14-day immersion, with corn rod-shaped crystal structures gathered around the tubules. In addition, visible mineralization occurred in partial areas of the CG group, rod-shaped crystals and in comparison, dentinal tubules in the PAMAM + CG group were almost completely covered by flaky crystal structures. Raman spectrum analysis showed that crystals formed by PAMAM, CG, and PAMAM + CG solution all had strong phosphate characteristic peaks, indicating the presence of hydroxyapatite (HA), that of the PAMAM + CG group was the strongest. The EDS results showed that the Ca and P levels of the PAMAM group and the CG group were slightly higher than those of the deionized water group, while PAMAM + CG group significantly higher than the others, Ca/P value approaching 1.67. The results of XRD showed the characteristic peaks of hydroxyapatite detected by the PAMAM + CG group at 2θ=26.0 (002), 2θ=32.0 (211), and 2θ=33.0 (112) were high and sharp, with a few diffraction line burrs indicating it had high crystallinity and purity. The Scherrer equation results showed that the appearance and size of the grains formed by the PAMAM + CG group were basically consistent with those of healthy dentin.
CONCLUSIONS
Altogether, the compound of polyamide-amine dendrimer and chlorhexidine could induce the remineralization of human dentin in a resin dentin bonding microenvironment with a gap of 50 µm to form a crystal structure similar to dentin hydroxyapatite.
PubMed: 33850869
DOI: 10.21037/atm-21-472 -
Biomaterial Investigations in Dentistry 2023To assess the influence of dentifrices with different abrasiveness levels on the properties of dental reconstructive materials. Forty-eight cylinders were obtained from...
To assess the influence of dentifrices with different abrasiveness levels on the properties of dental reconstructive materials. Forty-eight cylinders were obtained from four polymeric materials, being two CAD/CAM acrylic resins (Ivotion-Dent and Ivotion-Base), one injected acrylic resin (IvoBase-Hydrid) and one light-cured resin composite (Empress Direct). Specimens were allocated to four subgroups for toothbrushing simulation according to the dentifrice relative dentin abrasivity (RDA) and silica content: (i) RDA 0 = 0%; (ii) RDA 50 = 3%; (iii) RDA 100 = 10%; and (iv) RDA 120 = 25%. Specimens were then subjected to toothbrushing. Surface analyses [surface roughness Ra (SR) and scanning electron microscopy (SEM)] along with hardness and optical properties [translucency parameter (TP) and contrast ratio (CR)] were evaluated before and after toothbrushing. Statistical analyses were performed using ANOVA and Tukey test. A significant increase in SR was observed after toothbrushing with higher RDA toothpastes for Ivotion-Dent (100 and 120) and IvoBase-Hybrid (120). Ivotion-Base and Empress Direct presented no significant differences in SR when analyzed as a function of timepoint and RDA levels. Hardness was not influenced by toothbrushing with different RDA dentifrices, except for Empress Direct with RDA 0 toothpaste, where a decrease in the hardness was observed. TP of Ivotion-Dent and Empress Direct significantly decreased after toothbrushing with higher RDA dentifrices and CR of Ivotion-Dent, Empress Direct and IvoBase-Hybrid significantly increased with higher RDA dentifrices. The levels of dentifrice abrasiveness affected differently the SR, hardness and optical properties of polymeric reconstructive materials after toothbrushing.
PubMed: 38027422
DOI: 10.1080/26415275.2023.2268670 -
Journal of Dentistry Jan 2023To investigate the prevalence of tooth wear and associated factors in adults from the 1982 Pelotas Birth Cohort.
OBJECTIVES
To investigate the prevalence of tooth wear and associated factors in adults from the 1982 Pelotas Birth Cohort.
METHODS
A subsample of the 5,914 individuals from the 1982 Pelotas Birth Cohort was randomly selected to be interviewed and clinically examined at the age of 31. Tooth wear was evaluated using a simplified version of the Tooth Wear Index (TWI), considering the absence or presence of tooth wear in dentin of first molars and anterior teeth (incisors and canines). Independent variables were socioeconomic, demographic, unhealthy behavior, and mental health. Crude and adjusted Poisson regression models were employed. A significant level of P ≤ 0.05 was adopted.
RESULTS
A total of 537 individuals were evaluated. A tooth wear prevalence of 61.6% was observed. Females presented a statistically significant lower tooth wear in dentin prevalence ratio (PR) compared to males in the adjusted analysis (PR 0.76; 95% CI: 0.67-0.86). Acid beverage consumption (PR 1.22; 95% CI: 1.01-1.49) and consumption of alcoholic beverages (PR 1.19; 95% CI: 1.04-1.35) were positively associated with tooth wear.
CONCLUSION
Almost 2/3 of the surveyed individuals presented tooth wear in dentin. Being a male, higher consumption of alcohol and acidic beverages were factors associated with tooth wear.
CLINICAL SIGNIFICANCE
There was a high prevalence of tooth wear in dentin in the adult population. Data has also shown the etiological complexity of tooth wear, reinforcing the importance of an early diagnosis and the establishment of preventive measures to decrease the potentially hazardous effect of tooth wear over time.
Topics: Female; Male; Humans; Cohort Studies; Tooth Erosion; Prevalence; Tooth Wear; Tooth Attrition
PubMed: 36481129
DOI: 10.1016/j.jdent.2022.104386 -
Journal of Functional Biomaterials May 2024There is a vast amount of published literature concerning dental veneers; however, the effects of tooth preparation, aging, veneer type, and resin cement type on the... (Review)
Review
There is a vast amount of published literature concerning dental veneers; however, the effects of tooth preparation, aging, veneer type, and resin cement type on the failure of dental veneers in laboratory versus clinical scenarios are not clear. The purpose of the present narrative review was to determine the principal factors associated with failures of dental veneers in laboratory tests and to understand how these factors translate into clinical successes/failures. Articles were identified and screened by the lead author in January 2024 using the keywords ''dental veneer", "complication", "survival rate", "failure", and "success rate" using PubMed/Medline, Scopus, Google Scholar, and Science Direct. The inclusion criteria included articles published between January 1999 and January 2024 on the topics of preparation of a tooth, aging processes of the resin cement and veneer, translucency, thickness, fabrication technique of the veneer; shade, and thickness of the resin cement. The exclusion criteria included articles that discussed marginal and internal fit, microhardness, water sorption, solubility, polishability, occlusal veneers, retention, surface treatments, and wear. The results of the present review indicated that dental veneers generally have a high survival rate (>90% for more than 10 years). The amount of preserved enamel layer plays a paramount role in the survival and success rates of veneers, and glass-ceramic veneers with minimal/no preparation showed the highest survival rates. Fracture was the primary failure mechanism associated with decreased survival rate, followed by debonding and color change. Fractures increased in the presence of parafunctional activities. Fewer endodontic complications were associated with veneer restorations. No difference was observed between the maxillary and mandibular teeth. Fractures can be reduced by evaluation of occlusion immediately after cementation and through the use of high-strength veneer materials, resin cements with low moduli, and thin layers of highly polished veneers. Debonding failures can be reduced with minimal/no preparation, and immediate dentin sealing should be considered when dentin is exposed. Debonding can also be reduced by preventing contamination from blood, saliva, handpiece oil, or fluoride-containing polishing paste; through proper surface treatment (20 s of hydrofluoric acid etching for glass ceramic followed by silane for 60 s); and through use of light-cured polymerization for thin veneers. Long-term color stability may be maintained using resin cements with UDMA-based resin, glass ceramic materials, and light-cure polymerization with thin veneers.
PubMed: 38786642
DOI: 10.3390/jfb15050131 -
Polymers Mar 2023the aim of this study was to examine the stress distribution of enamel, dentin, and restorative materials in sound first molar teeth with restored cavities with...
Teeth Restored with Bulk-Fill Composites and Conventional Resin Composites; Investigation of Stress Distribution and Fracture Lifespan on Enamel, Dentin, and Restorative Materials via Three-Dimensional Finite Element Analysis.
OBJECTIVES
the aim of this study was to examine the stress distribution of enamel, dentin, and restorative materials in sound first molar teeth with restored cavities with conventional resin composites and bulk-fill composites, as well as to determine their fracture lifetimes by using the three-dimensional finite element stress analysis method.
MATERIALS AND METHODS
an extracted sound number 26 tooth was scanned with a dental tomography device and recorded. Images were obtained as dicom files, and these files were transferred to the Mimics 12.00 program. In this program, different masks were created for each tooth tissue, and the density thresholds were adjusted manually to create a three-dimensional image of the tooth, and these were converted to a STL file. The obtained STL files were transferred to the Geomagic Design X program, and some necessary adjustments, such as smoothing, were made, and STP files were created. Cavity preparation and adhesive material layers were created by transferring STP files to the Solidworks program. Finally, a FE model was created in the ABAQUS program, and stress distributions were analyzed.
RESULTS
when the bulk-fill composite and conventional resin composite materials were used in the restoration of the cavity, the structures that were exposed to the most stress as a result of occlusal forces on the tooth were enamel, dentin, restorative material, and adhesive material. When the bulk-fill composite material was used in restoration, while the restorative material had the longest fracture life as a result of stresses, the enamel tissue had the shortest fracture life. When the conventional resin composite material was used as the restorative material, it had the longest fracture life, followed by dentin and enamel.
CONCLUSION
when the bulk-fill composite material was used instead of the conventional resin composite material in the cavity, the stress values on enamel, dentin, and adhesive material increased as a result of occlusal forces, while the amount of stress on the restorative material decreased. In the fracture analysis, when the bulk-fill composite material was used instead of the conventional resin composite material, a decrease in the number of cycles required for the fracture of enamel, dentin, and restorative materials was observed as a result of the forces generated in the oral cavity.
PubMed: 37050251
DOI: 10.3390/polym15071637