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Mikrobiyoloji Bulteni Oct 2019Aim of the present study was to identify protozoones which are difficult to define through wet slide in fresh fecal samples by using different fixatives with modified...
Aim of the present study was to identify protozoones which are difficult to define through wet slide in fresh fecal samples by using different fixatives with modified Trichrome stain within five minutes. Two different fixatives prepared for the alternative approach. The slides were fixed by two different fixatives, one of them (fixative-1) was based ethylalcohol, formalin, acetic acid, distilled water and the other one (fixative-2) based ethylalcohol, formalin, citric acid, distilled water included a mordant [divalent or polyvalent metals which make coordination complex with some dyes] consisted copper sulphate pentahydrate (CuSO4 .5H2 O). Slides prepared by the two different fixatives were stained by a different modification of Gomori's trichrome stain that we made. Samples fixed by Schaudinn fixative including mercury chloride were stained by Wheatley modification of Gomori's trichrome stain as a gold standard for control and comparison. We worked with 50 fecal samples which we thought included human intestinal protozoones after the wet slide examination. Comparing the methods, slides prepared with the method including citric acid gave almost similar results with the classical method excluded Entamoeba coli cystes. Slides prepared with the methode including acetic acid gave low performance compared with the classical method especially E.coli cystes and Blastocystis spp., Endolimax nana, Iodamoeba bütschlii, E.hartmanni. Both new fixatives gave superior performance at the slides included Dientamoeba fragilis and approximately shorten the procedure process ten times than the classical method. When the both alternative methods compared in each other, the slides prepared with fixative-2 exposed better performance for the protozoones Blastocystis spp., E.nana, I.bütschlii and E.hartmanni while the fixative-1 displayed minimal superiority for D.fragilis including criterias that we based. The fixative-2 and modified stain methode that we used in our study, makes available the diagnostic phase ten times faster than the classical method in human stool parasitological tests excluding the E.coli cystes at parasitology and microbiolgy laboratories. It seems to be a good option to the classical method for routine usage.
Topics: Eukaryota; Feces; Fixatives; Formaldehyde; Humans; Microscopy; Parasitic Diseases; Parasitology
PubMed: 31709939
DOI: 10.5578/mb.68633 -
New Microbes and New Infections Apr 2024Gastrointestinal pathogens (GPs) contribute significantly to the burden of illness worldwide with diarrhoea being the most common among gastrointestinal symptoms (GSs)....
BACKGROUND
Gastrointestinal pathogens (GPs) contribute significantly to the burden of illness worldwide with diarrhoea being the most common among gastrointestinal symptoms (GSs). In the COVID-19 disease, diarrhoea, could be one of the initial presenting symptoms. However, no data on the potential correlation between diarrhoea-causing pathogens and SARS-CoV-2 infection are available. Therefore, we carried out a 2-years retrospective study aimed to evaluate the prevalence of "classic" GPs among SARS-CoV-2 infected and non-infected patients with diarrhoea in Italy.
METHODS
Results of SARS-CoV-2 research from nasopharyngeal and detection of GPs from stool swab samples by Allplex™ SARS-CoV-2 and GI Virus, Bacteria and Parasite Assay were analysed for all patients with diarrhoea referring to Policlinico Ospedaliero Universitario, Foggia, (Italy) from February 2022 to October 2023.
RESULTS
Out of the 833 involved patients, 81 (3.9%) were COVID-19 positive, while 752 (90.3%) were COVID-19 negative. Among COVID-19-positive patients, 37% (n = 30/81) were found positive for one or more GPs with a higher prevalence of protozoan parasites (18.5%) ( ST1-ST4 subtypes, genotype I), followed by bacteria (7.4%) ( sp., sp.). Viral pathogens were more frequent among COVID-19 negative patients (Adenovirus, Norovirus). Among GPs, ST3 subtype was the most prevalent registered in the 16% of patients (p = 0.0001).
CONCLUSIONS
Based on obtained results, a likely interaction between the classic GPs and SARS-CoV-2 infection can be speculated, driven by protozoan parasites. Moreover, these results also provide baseline data to understand more deeply sp. role in this scenario of dysbiosis, particularly in those cases of SARS-CoV-2 co-infection.
PubMed: 38406386
DOI: 10.1016/j.nmni.2024.101228 -
Revista Espanola de Quimioterapia :... Feb 2023Microscopic examination of the intestinal parasites, from the patient's concentrated feces, has a lower sensitivity when compared to molecular diagnostic techniques....
OBJECTIVE
Microscopic examination of the intestinal parasites, from the patient's concentrated feces, has a lower sensitivity when compared to molecular diagnostic techniques. Therefore, the objective of this study has been to compare both techniques, as well as to evaluate whether there is a correlation between the microscopic examination and the threshold cycles (Ct) obtained for Blastocystis hominis.
METHODS
Retrospective study of the samples received in the Microbiology laboratory during September 2021. The MiniParasep SF® concentration test was performed for microscopic visualization and then PCR was performed with the Seegene AllplexTM Parasite Assay panel.
RESULTS
A 27% (n=74) of the samples were positive by molecular diagnosis, with a total of 87 parasites detected. 53% (n=39) were women with a mean age of 47 ± 24 years. In 76% (n=56) of the cases the service of origin was Primary Care. The most frequently found parasite was B. hominis, 85% (n=64), followed by Dientamoeba fragilis 20% (n=15) and Giardia lamblia 11% (n=8). Co-infection by two parasites was detected in 13 cases (B. hominis + D. fragilis in 6 cases, and B. hominis + G. lamblia in 7 cases). In the microscopic diagnosis, 9.5% (n=26) positivity was obtained. The most frequently found parasite was B. hominis, 84% (n=23), followed by G. lamblia, which was seen in three cases by microscopy. D. fragilis was not seen in any case. Coinfection of B. hominis + G. lamblia was observed in one sample.
CONCLUSIONS
Techniques for molecular diagnosis of intestinal parasites are fast, reliable and more sensitive than microscopic techniques, improving microbiological diagnosis and quality of care.
Topics: Humans; Female; Young Adult; Adult; Middle Aged; Aged; Male; Retrospective Studies; Microscopy; Intestinal Diseases, Parasitic; Giardia lamblia; Feces; Molecular Biology
PubMed: 36453171
DOI: 10.37201/req/088.2022 -
Pathogens (Basel, Switzerland) Mar 2024Intestinal parasitic infections are one of the most common infectious diseases worldwide, particularly in developing countries. A distinct group at increased risk of...
Intestinal parasitic infections are one of the most common infectious diseases worldwide, particularly in developing countries. A distinct group at increased risk of infection is military personnel deployed overseas for extended periods, typically six months at a time. The aim of this study was to determine the prevalence of spp. and other intestinal parasites in Polish military personnel returning from deployments to Lebanon (n = 206) and Iraq (n = 220). In this group of subjects, we found spp. (13.6%), (3.3%), (0.9%), and (0.5%). sensu lato and infections were detected only in one soldier returning from Lebanon and Iraq, respectively. subtype (ST) 3 was predominant in soldiers returning from Lebanon, followed by ST2 and ST1. ST1 infection was predominant in soldiers returning from Iraq, followed by ST3 and ST2. Our study affirms that, deployment abroad is of no influence of the prevalence of parasitic protozoa. However, it would be worth to monitor parasite infection in military personnel returning from tropical zone even if they have no actual symptoms. In addition, it is very important to determine the subtypes of -this may help to clearly define their pathogenicity, especially considering the scarcity of studies on genotypes in Iraqi and Lebanese residents.
PubMed: 38535614
DOI: 10.3390/pathogens13030271