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In Vivo (Athens, Greece) 2020Development of malignant tumors is preceded by molecular biological events. Our aim was to establish an assay panel by using miRNAs and other genes for the rapid...
BACKGROUND/AIM
Development of malignant tumors is preceded by molecular biological events. Our aim was to establish an assay panel by using miRNAs and other genes for the rapid screening of potential carcinogens or chemopreventive agents.
MATERIALS AND METHODS
Six male and 6 female CBA/Ca mice received 20 mg/bwkg 7,12-dimethylbenz(α)anthracene (DMBA) intraperitoneally, and 24 h later RNA was isolated from parenchymal organs. Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and mTORC1 was analysed by real time polymerase chain reaction and compared to non-treated controls.
RESULTS
DMBA caused significant alterations in the expression of the studied genes. The most profound changes were the strongly elevated miR-9-3 and mTORC1 expressions in female mice in all organs studied.
CONCLUSION
miR-9-3 and mTORC1 expression in female mice were found to be the most suitable biomarkers for rapid identification of possible carcinogenic effects.
Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Anthracenes; Carcinogens; Female; Male; Mechanistic Target of Rapamycin Complex 1; Mice; Mice, Inbred CBA; MicroRNAs
PubMed: 32871758
DOI: 10.21873/invivo.12046 -
Molecules (Basel, Switzerland) Jul 2020essential oil and carvacrol have shown an antitumor effect on breast tumor cell lines; the main objective of this research was to evaluate the antitumor effect of the...
essential oil and carvacrol have shown an antitumor effect on breast tumor cell lines; the main objective of this research was to evaluate the antitumor effect of the essential oil of (EOCc) and carvacrol on 7,12-dimethylbenz [a] anthracene (DMBA)-induced breast cancer in female rats. Cancer was induced by a single administration of DMBA at dose of 80 mg/kg body weight (BW). A total of 54 female Holtzman rats were randomly assigned into 9 groups (n = 6). Group I: PS (Physiological saline); Group II: DMBA; Groups III, IV, and V: DMBA + EOCc at doses of 50, 100 and 200 mg/kg/day BW, respectively; Groups VI, VII, and VIII: DMBA + carvacrol at doses of 50, 100 and 200 mg/kg/day BW, respectively; and group IX: DMBA + EOCc + carvacrol at doses of 100 mg/kg/day BW. The treatment lasted 14 weeks. As results, EOCc showed a reduction in tumors as well as necrosis and mitosis. Animals treated with carvacrol did not show necrosis, mitosis, or infiltration. Carvacrol at dose of 100 mg/kg/day BW revealed a significant decrease in the cumulative tumor volume down to 0.11 ± 0.05 cm compared to 0.38 ± 0.04 cm of the DMBA group ( < 0.01). It is concluded that EOCc and carvacrol had an antitumor effect on DMBA-induced breast cancer in female rats.
Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Antioxidants; Biphenyl Compounds; Body Weight; Carcinogenesis; Cymbopogon; Cymenes; Female; Mammary Neoplasms, Experimental; Oils, Volatile; Picrates; Rats, Sprague-Dawley
PubMed: 32698395
DOI: 10.3390/molecules25143284 -
In Vivo (Athens, Greece) 2021The aim of the study was to assess the impact of nano-, micro-, and macro-sized-genistein on the growth and development of neoplasms in rats with mammary cancer....
BACKGROUND/AIM
The aim of the study was to assess the impact of nano-, micro-, and macro-sized-genistein on the growth and development of neoplasms in rats with mammary cancer. Additionally, the effect on the kinetics of changes (9-11-17-20 week of a rat's life) in the levels of methyl derivatives: 1-methyladenine, 3-methyladenine, 7-methylguanine, 1-methylguanine, 1-methyladenosine, 7-methylguanosine, O-methyl-guanosine and N6-methyl-2'-deoxyguanosine in the urine of rats was analyzed.
MATERIALS AND METHODS
Female Sprague-Dawley rats divided into 4 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano-, micro- and macro-sized genistein. To induce the mammary adenocarcinoma, rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a high-performance liquid chromatography coupled to mass spectrometry method (LC-MS/MS).
RESULTS
The supplementation of the diet of animals with genistein resulted in an increase in the excretion of methylated derivatives in the urine of rats. In the animals receiving standard diet, the levels of methyl derivatives increased during the study or remained relatively low. In the case of animals whose diet was supplemented with the various forms of genistein, the levels of methylated derivatives were very high from the beginning.
CONCLUSION
High levels of methyl derivatives can influence carcinogenesis.
Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Chromatography, Liquid; Dietary Supplements; Female; Genistein; Mammary Neoplasms, Experimental; Nucleosides; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry
PubMed: 34182481
DOI: 10.21873/invivo.12475 -
Nutrients Aug 2019Breast cancer and cardiovascular diseases (CVD) have shared risk factors and mechanisms of pathogenicity, as proven by increased cardiac risk in breast cancer patients...
Conjugated Linoleic Acid Isomers Affect Profile of Lipid Compounds and Intensity of Their Oxidation in Heart of Rats with Chemically-Induced Mammary Tumors-Preliminary Study.
Breast cancer and cardiovascular diseases (CVD) have shared risk factors and mechanisms of pathogenicity, as proven by increased cardiac risk in breast cancer patients receiving anticancerogenic therapies and in cancer survivors. A growing mammary tumor may cause heart injury in cancer patients who have not yet been treated. This study aimed to evaluate the effect of conjugated linoleic acid (CLA) supplementation of female rats with 7,12-dimethylbenz(a)anthracene (DMBA)-induced cancerogenesis on fatty acids (FAs), conjugated FAs (CFAs), malondialdehyde (MDA), cholesterol and oxysterols content in cardiac tissue. FAs, cholesterol and oxysterols contents were determined by gas chromatography coupled with mass spectrometry, while the contents of CFAs and MDA were determined by high performance liquid chromatography with photodiode detection. Our results indicate that both CLA supplementation and the presence of tumors influence the lipid biomarkers of CVD. A significant interaction of both experimental factors was observed in the content of polyunsaturated FAs (PUFAs), n-6 PUFAs and CFAs. CLA supplementation significantly inhibited PUFA oxidation, as evidenced by the lower content of MDA in rats' hearts, while the cancerous process intensified the oxidation of cholesterol, as confirmed by the elevated levels of 7-ketocholesterol in DMBA-treated rats. These results may significantly expand knowledge about CLA properties in terms of the prevention of co-existing non-communicable diseases.
Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Dietary Supplements; Female; Heart; Heart Diseases; Isomerism; Linoleic Acids, Conjugated; Lipid Peroxidation; Mammary Neoplasms, Experimental; Oxidation-Reduction; Rats
PubMed: 31480307
DOI: 10.3390/nu11092032 -
Asian Journal of Surgery Feb 2024
Topics: Rats; Animals; Electroacupuncture; Carcinogenesis; 9,10-Dimethyl-1,2-benzanthracene
PubMed: 38036366
DOI: 10.1016/j.asjsur.2023.11.080 -
BioMed Research International 2020To investigate the effect of systemic administration of the immunosuppressant dexamethasone (DM) while inducing hamster buccal pouch DMBA carcinogenesis. . Two different...
OBJECTIVES
To investigate the effect of systemic administration of the immunosuppressant dexamethasone (DM) while inducing hamster buccal pouch DMBA carcinogenesis. . Two different experiments were performed. In the first experiment, hamsters' right buccal pouches in group A ( = 10) were painted three times per week with 7,12-dimethylbenzanthracene (DMBA) 0.5%, while pouches of animals in group B ( = 10) were painted three times per week with 7,12-dimethylbenzanthracene (DMBA) 0.5%, while pouches of animals in group B (.
RESULTS
The time of macroscopic neoplasm development was reduced when DM-DMBA coexposition was employed, finding tumors after 10-12 weeks of exposition. In addition, the frequency of histopathological lesions was higher.
CONCLUSION
Immunomodulatory action of dexamethasone may reduce the time of oral squamous cell carcinoma (OSCC) induction and may increase the incidence of neoplasms developed.
Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Carcinogens; Carcinoma, Squamous Cell; Cricetinae; Dexamethasone; Male; Mesocricetus; Mouth Mucosa; Mouth Neoplasms
PubMed: 32149076
DOI: 10.1155/2020/1470868 -
Nutrients Jul 2020Natural products have attracted great interest for some time as alternative methods against cancers by fulfilling immunomodulating properties. In this study, we...
Natural products have attracted great interest for some time as alternative methods against cancers by fulfilling immunomodulating properties. In this study, we investigated the activity of hot water extracts (120 °C, >30 min) of fresh leaves of Kumaizasa bamboo and Chaga mushroom which we called MeshimaMax, for cancer prevention and treatment by using different solid tumor models. In the implanted mouse sarcoma S180 tumor, MeshimaMax treatment significantly inhibited tumor growth when it was applied at the early stage of tumor inoculation. The effect was further confirmed by using carcinogen induced tumors, i.e., azoxymethane (AOM)/dextran sulfate sodium (DSS) induced mouse colon cancer and 7,12-dimethylbenz anthracene (DMBA) induced rat breast cancer. In both cases the occurrences of tumors were remarkably suppressed by administration of MeshimaMax which consists of three components above. More importantly, when MeshimaMax was combined with an anticancer chemotherapeutic drug, the therapeutic effect was remarkably improved. In vitro studies showed that when MeshimaMax was applied to mouse macrophage RAW264.7 cells the phagocytosis of macrophages was significantly activated, which was evaluated by using living yeast cells as well as synthetic nanoparticles. A cytotoxicity assay showed the 50% inhibitory concentration (IC) was higher than 1 mg/mL and normal cells were 2-3 times more tolerant to MeshimaMax than cancer cells. These findings suggest the potential application of MeshimaMax for cancer prevention and as supplement regimen for anticancer chemotherapy, probably functioning through activation of innate immunity, which may benefit cancer patients as an alternative supplement.
Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Antineoplastic Combined Chemotherapy Protocols; Azoxymethane; Breast Neoplasms; Colonic Neoplasms; Dextran Sulfate; Disease Models, Animal; Female; Immunity, Innate; Inonotus; Macrophages; Male; Mice; Phagocytosis; Phellinus; Plant Extracts; Plant Leaves; RAW 264.7 Cells; Rats; Sarcoma 180; Sasa
PubMed: 32751371
DOI: 10.3390/nu12082279 -
Experimental Biology and Medicine... Feb 2020-mannose-sensitive hemagglutinin (PAM) is an inactivate with mannose-sensitive hemagglutinin. Recently, the anticancer properties of PAM against many cancers have been...
UNLABELLED
-mannose-sensitive hemagglutinin (PAM) is an inactivate with mannose-sensitive hemagglutinin. Recently, the anticancer properties of PAM against many cancers have been reported across a range of studies. However, the exact mechanism through which PAM prevents skin cancer remains unclear. The aim of this study is to show to what extent PAM could inhibit the dimethylbenzanthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin cancer. JB6 cells were treated by TPA so as to establish an model. The effects of PAM on proliferation of the cells were analyzed using cell counting kit-8 assays. Effects on epithelial–mesenchymal transition (EMT) were assayed by real-time PCR and Western blotting. A DMBA/TPA-induced skin cancer mouse model was also established. The results showed that TPA promoted EMT changes through the activation of the hedgehog (Hh) pathway, which was reversed by PAM. Moreover, PAM inhibited the cancer growth and Hh pathway . These data indicate that PAM may serve as a potential anticancer agent for the treatment of skin cancer.
IMPACT STATEMENT
-mannose-sensitive hemagglutinin (PAM) restrained the chemical-induced skin cancer cells and partly through suppressing the Hh signaling pathway, indicating that PAM may be a promising anticancer agent for treating skin cancer.
Topics: Epithelial-Mesenchymal Transition; Fimbriae Proteins; Hedgehog Proteins; Hemagglutinins; Humans; Mannose; Pseudomonas aeruginosa; Skin Neoplasms
PubMed: 31903775
DOI: 10.1177/1535370219897240