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Saudi Journal of Ophthalmology :... 2022Aqueous flare and cells are inflammatory parameters of anterior chamber inflammation resulting from disruption of the blood-ocular barrier. The ocular inflammation...
Aqueous flare and cells are inflammatory parameters of anterior chamber inflammation resulting from disruption of the blood-ocular barrier. The ocular inflammation related to anterior chamber cells and flare is assessed by conventional clinical grading systems through using the slit-lamp examination. However, a more quantitative and objective assessment is needed for more precise and reproducible inflammatory assessment in uveitis. Laser flare photometer (LFP) was introduced as a noninvasive, objective, and quantitative evaluation of aqueous flare intensity and number of cells in the anterior chamber with good accuracy and repeatability. The success of LFP allowed clinicians to further evaluate the pathophysiology of intra-ocular inflammation and to incorporate LFP measurements to their routine clinical practice for diagnosis, management, and treatment of uveitis cases. In this review, we will discuss the importance of clinical utilization of LFP and the correlation between LFP and clinical grading systems along with some technical aspects. Furthermore, we will give a literature summary on the current applications of LFP in clinical practice of cases which present with various types of uveitis and diverse ocular conditions with or without inflammation.
PubMed: 36618569
DOI: 10.4103/sjopt.sjopt_119_22 -
Diagnostics (Basel, Switzerland) Jan 2023This article systematizes available data from the literature on biliary gastritis (BG) in order to increase the awareness of specialists about the latest possibilities... (Review)
Review
This article systematizes available data from the literature on biliary gastritis (BG) in order to increase the awareness of specialists about the latest possibilities for diagnosing the disease. BG occurs as a result of pathological duodenogastric reflux. In patients with a preserved duodenogastric junction, the dominant factor is represented by motor disorders of the upper digestive tract (primary biliary gastritis), while in patients recovering from surgical interventions it is represented by structural changes (secondary biliary gastritis). Progressive BG can lead to atrophy of the gastric mucosa, intestinal metaplasia, epithelial dysplasia, and eventually to gastric cancer. Diagnostic methods for BG are carried out to identify risk factors, exclude alarm symptoms and identify persistent motor disorders and pathological reflux (24 h pH-impedancemetry, hepatobiliary scintigraphy, 24 h monitoring of bilirubin content in the reflux using a Bilitec 2000 photometer), as well as to diagnose gastritis itself (esophagogastroduodenoscopy, morphological gastrobiopsy examination). The diagnosis of BG should be based on a multidisciplinary approach that combines a thorough analysis of a patient's complaints, an anamnesis of the disease, and the results of endoscopic and histological research methods.
PubMed: 36672996
DOI: 10.3390/diagnostics13020186 -
Scientific Reports Oct 2022The growing concern about the negative impact of artificial light at night on biodiversity and human health increases the need of defining a general indicator that could...
The growing concern about the negative impact of artificial light at night on biodiversity and human health increases the need of defining a general indicator that could be used for characterizing light pollution as well as performing both spatial and temporal comparisons. In this paper, we show that the traditional indicators based on direct numerical measurements of sky brightness suffer from significant limitation due to calibration bias and lack of reproducibility. Furthermore, these measures are most often performed in periods of clear sky. They do not reflect the wide variety of meteorological conditions that can produce highly inhomogeneous levels of light pollution on a given site. To overcome these issues, we propose a statistical indicator called NSB Dispersion Ratio. This indicator is derived from a statistically significant number of individual night sky brightness measurements, under various meteorological conditions. It is independent of any absolute photometer calibration. It only requires on-time precise corrections of the contribution of natural light sources such as the Galactic plane.
Topics: Calibration; Humans; Light Pollution; Reproducibility of Results
PubMed: 36220856
DOI: 10.1038/s41598-022-21460-5 -
Current Research in Biotechnology 2022We catalog and summarize evidence of the analytical performance of portable quantitative and semi-quantitative devices for the assessment of vitamin A status and vitamin... (Review)
Review
BACKGROUND
We catalog and summarize evidence of the analytical performance of portable quantitative and semi-quantitative devices for the assessment of vitamin A status and vitamin A deficiency (VAD) in various biological samples-including whole blood, plasma, serum, and milk-in addition to VAD determination by functional indicators such as pupillary response.
METHODS
We searched the literature for published research articles, patents, and information from manufacturers of mobile devices, particularly those appropriate for low-resource settings. The included devices were required to be portable (lightweight and ideally not needing a power outlet) and to measure vitamin A as well as define VAD. Eligible studies compared a portable device to a reference standard of high-performance liquid chromatography for blood and milk, or a Goldmann-Weekers dark adaptometer for eyes/vision. Where available, identified devices were compared with reference methods across several performance criteria. When possible, we compared the device's performance reported in published studies against the stated performance criteria from the manufacturers' websites.
RESULTS
We catalogued 25 portable devices for measuring vitamin A and/or VAD via biological samples. We also identified 18 comparison studies (plus associated reports) assessing nine methods: the iCheck Fluoro, iCheck Carotene, CRAFTi, Tidbit with or without the HYPER filtration system, custom field-friendly immunoassays, and microfluidic assays for blood; the iCheck Fluoro and iCheck Carotene for milk; and the Scotopic Sensitivity Tester-1 for eye function.
CONCLUSIONS
The iCheck Fluoro and iCheck Carotene are commercially available for use and are acceptable for measuring vitamin A in blood and milk samples, according to the available validation data. Many of the other identified devices, including other portable fluorometers, photometers, immunoassays, microfluidics-based devices, and dark adaptometers, were proofs of concept and not yet commercially available. Furthermore, none of these other devices included manufacturer-described device performance criteria to compare with descriptions from experimental studies. Several gaps remain, including studies comparing the other portable devices against a reference standard, particularly for functional indicators of vitamin A status/deficiency; available manufacturer-reported device performance criteria against which to compare future results of investigations; and more comprehensive reporting of validation metrics including sensitivity, specificity, precision, and Bland-Altman analysis.
PubMed: 36033130
DOI: 10.1016/j.crbiot.2022.04.003 -
Scientific Reports Oct 2023Photoelectric observations of night sky brightness (NSB) at different zenith distances and azimuths, covering all the sky, at the Egyptian Kottamia Astronomical...
Photoelectric observations of night sky brightness (NSB) at different zenith distances and azimuths, covering all the sky, at the Egyptian Kottamia Astronomical observatory (KAO) site of coordinates ϕ = 29° 55.9' N and λ = 31° 49.5' E, were done using a fully automated photoelectric photometer (FAPP). The Bessel wide range system (UBVRI) is used for the first time to observe NSB for three consecutive nights (1-3 August, 2022) under good seeing conditions after the moon sets. The deduced results were taken in photons and converted into mag/arcsec. The average zenith sky brightness for U, B, V, R and I filters are found to be 20.49, 20.38, 19.41, 18.60 and 17.94 mag/arcsec respectively. The average color indices (U-B), (B-V), (V-R) and (R-I), at the zenith are detected to be 0.11, 0.98, 0.81 and 0.66, respectively. We plotted the isophotes of the sky brightness at KAO in U, B, V, R and I colors (filters) and determined both the average atmospheric extinction and sky transparency through these UBVRI filters. The atmospheric and other meteorological conditions were taken into our consideration during the observational nights. The results of the current study illustrate the main impact of the new cities built around KAO on the sky glow over it, and which astronomical observations are affected.
PubMed: 37798327
DOI: 10.1038/s41598-023-43844-x -
International Journal of Environmental... Apr 2022This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included the analysis of...
This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included the analysis of airborne dust (laser photometer), the number of microorganisms in the air, soil and leachate (culture method) and the microbial diversity in the landfill environment (high-throughput sequencing on the Illumina Miseq); the cytotoxicity () and genotoxicity (alkaline comet assay) of soil and leachate were tested. Moreover, an analysis of UHPLC-Q-ToF-UHRMS (ultra-high-performance liquid chromatography-quadrupole-time-of-flight ultrahigh-resolution mass spectrometry) was performed to determine the toxic compounds and microbial metabolites. The PM dust fraction constituted 99.89% and 99.99% of total dust and exceeded the threshold of 0.025 mg m at the tested locations. In the air, the total number of bacteria was 9.33 × 10-1.11 × 10 CFU m, while fungi ranged from 1.17 × 10 to 4.73 × 10 CFU m. Psychrophilic bacteria were detected in the largest number in leachates (3.3 × 10 to 2.69 × 10 CFU mL) and in soil samples (8.53 × 10 to 1.28 × 10 CFU g). Bacteria belonging to Proteobacteria (42-64.7%), Bacteroidetes (4.2-23.7%), Actinobacteria (3.4-19.8%) and Firmicutes (0.7-6.3%) dominated. In the case of fungi, Basidiomycota (23.3-27.7%), Ascomycota (5.6-46.3%) and Mortierellomycota (3.1%) have the highest abundance. Bacteria (, , , , ) and fungi (, , , , , , , , , ) that are potentially hazardous to human health were detected in samples collected from the landfill. Tested leachates and soils were characterised by varied cyto/genotoxins. Common pesticides (carbamazepine, prometryn, terbutryn, permethrin, carbanilide, pyrethrin, carbaryl and prallethrin), quaternary ammonium compounds (benzalkonium chlorides), chemicals and/or polymer degradation products (melamine, triphenylphosphate, diphenylphtalate, insect repellent diethyltoluamide, and drugs (ketoprofen)) were found in soil and leachate samples. It has been proven that the tested landfill is the source of the emission of particulate matter; microorganisms (including potential pathogens) and cyto/genotoxic compounds.
Topics: Air Microbiology; Bacteria; Dust; Fungi; Humans; Poland; Soil; Waste Disposal Facilities
PubMed: 35457694
DOI: 10.3390/ijerph19084826 -
Sensors (Basel, Switzerland) May 2021Using a solar radiometer is an effective approach for improving the remote sensing of solar irradiance distribution and atmospheric composition. Long-term development of...
Using a solar radiometer is an effective approach for improving the remote sensing of solar irradiance distribution and atmospheric composition. Long-term development of a solar scanning radiometer enables frequent and reliable measurement of atmospheric parameters such as the water vapor column and aerosol optical properties. However, the discrete wavelength radiometer has encountered a bottleneck with respect to its insufficient spectral resolution and limited observation waveband, and it has been unable to satisfy the needs of refined and intelligent on-site experiments. This study proposes a solar-skylight spectroradiometer for obtaining visible and near-IR fine spectrum with two types of measurement: direct-sun irradiance and diffuse-sky radiance. The instrument adopts distributed control architecture composed of the ARM-Linux embedded platform and sensor networks. The detailed design of the measuring light-path, two-axis turntable, and master control system will be addressed in this study. To determine all coefficients needed to convert instrument outputs to physical quantities, integrating sphere and Langley extrapolation methods are introduced for diffuse-sky and direct-sun calibration, respectively. Finally, the agreement of experimental results between spectroradiometers and measuring benchmarks (DTF sun-photometer, microwave radiometer, and Combined Atmospheric Radiative Transfer simulation) verifies the feasibility of the spectroradiometer system, and the radiation information of feature wavelengths can be used to retrieve the characteristics of atmospheric optics.
PubMed: 34071400
DOI: 10.3390/s21113751 -
Scientific Reports Sep 2023In 1924, the CIE published and standardized the photopic luminous efficiency function. Based on the standardized curve, luminous flux in lumens, luminance in...
In 1924, the CIE published and standardized the photopic luminous efficiency function. Based on the standardized curve, luminous flux in lumens, luminance in cd/m[Formula: see text], and illuminance in lux are determined by an integral of the curve and the incident light spectra in photometers and are considered physical brightness. However, human brightness perception is not only weighted by this simple determination, but is a more complicated combination of all L-cones, M-cones, S-cones, rods and later ipRGCs, which was partly described by the equivalent brightness of Fotios et al. with the correction factor [Formula: see text]. Recently, new research has demonstrated the role of ipRGCs in human light perception. However, it is still unclear how these signal components of the human visual system are involved in the overall human brightness perception. In this work, human brightness perception under photopic conditions was investigated by visual experiments with 28 subjects under 25 different light spectra. In this way, the contributions of the signal components can be investigated. An optimization process was then performed on the resulting database. The results show that not only the [Formula: see text] component, but also the S-cones and ipRGC play a role, although it is smaller. Thus, the visually scaled brightness model based on the database optimization was constructed using not only illuminance but also S-cones and ipRGC with [Formula: see text] of 0.9554 and RMSE of 4.7802. These results are much better than the brightness model after Fotios et al. using only S-cones ([Formula: see text] = 0.8161, RMSE = 9.7123) and the traditional model without S-cones and ipRGC ([Formula: see text] = 0.8121, RMSE = 9.8171).
Topics: Humans; Color Vision; Visual Perception; Retinal Cone Photoreceptor Cells; Databases, Factual; Light
PubMed: 37666893
DOI: 10.1038/s41598-023-41084-7 -
Bio-protocol May 2022Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth...
Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution, higher efficiency, and are less laborious, while analysis and interpretation of the microplate-based measurements are less straightforward. Recently, we developed a new analysis method for evaluating bacterial growth with microplate readers based on time derivatives. Here, we describe a detailed protocol for this development and provide the homemade program for the new analysis method.
PubMed: 35800461
DOI: 10.21769/BioProtoc.4410 -
Optics Express Feb 2023Aerosol microphysical properties, such as volume concentration (VC) and effective radius (ER), are of great importance to evaluate their radiative forcing and impacts on...
Aerosol microphysical properties, such as volume concentration (VC) and effective radius (ER), are of great importance to evaluate their radiative forcing and impacts on climate change. However, range-resolved aerosol VC and ER still cannot be obtained by remote sensing currently except for the column-integrated one from sun-photometer observation. In this study, a retrieval method of range-resolved aerosol VC and ER is firstly proposed based on the partial least squares regression (PLSR) and deep neural networks (DNN), combining polarization lidar and collocated AERONET (AErosol RObotic NETwork) sun-photometer observations. The results show that the measurement of widely-used polarization lidar can be reasonably used to derive the aerosol VC and ER, with the determination coefficient (R) of 0.89 (0.77) for VC (ER) by use of the DNN method. Moreover, it is proven that the lidar-based height-resolved VC and ER at near-surface are well consistent with independent observations of collocated Aerodynamic Particle Sizer (APS). Additionally, we found that there are significant diurnal and seasonal variations of aerosol VC and ER in the atmosphere at Semi-Arid Climate and Environment Observatory of Lanzhou University (SACOL). Compared with columnar ones from the sun-photometer observation, this study provides a reliable and practical way to obtain full-day range-resolved aerosol VC and ER from widely-used polarization lidar observation, even under cloud conditions. Moreover, this study also can be applied to long-term observations by current ground-based lidar networks and spaceborne CALIPSO lidar, aiming to further evaluate aerosol climatic effects more accurately.
PubMed: 36859889
DOI: 10.1364/OE.481252