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Cell Sep 2022Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range...
Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.
Topics: Animals; Interneurons; Mice; Microscopy; Neurons; Photons; Wakefulness
PubMed: 35985322
DOI: 10.1016/j.cell.2022.07.013 -
Biosensors Dec 2022Bound states in the continuum (BICs) have attracted considerable attentions for biological and chemical sensing due to their infinite quality ()-factors in theory. Such... (Review)
Review
Bound states in the continuum (BICs) have attracted considerable attentions for biological and chemical sensing due to their infinite quality ()-factors in theory. Such high- devices with enhanced light-matter interaction ability are very sensitive to the local refractive index changes, opening a new horizon for advanced biosensing. In this review, we focus on the latest developments of label-free optical biosensors governed by BICs. These BICs biosensors are summarized from the perspective of constituent materials (i.e., dielectric, metal, and hybrid) and structures (i.e., grating, metasurfaces, and photonic crystals). Finally, the current challenges are discussed and an outlook is also presented for BICs inspired biosensors.
Topics: Photons
PubMed: 36551087
DOI: 10.3390/bios12121120 -
Chemical Reviews Oct 2022Recent years have witnessed promising artificial intelligence (AI) applications in many disciplines, including optics, engineering, medicine, economics, and education.... (Review)
Review
Recent years have witnessed promising artificial intelligence (AI) applications in many disciplines, including optics, engineering, medicine, economics, and education. In particular, the synergy of AI and meta-optics has greatly benefited both fields. Meta-optics are advanced flat optics with novel functions and light-manipulation abilities. The optical properties can be engineered with a unique design to meet various optical demands. This review offers comprehensive coverage of meta-optics and artificial intelligence in synergy. After providing an overview of AI and meta-optics, we categorize and discuss the recent developments integrated by these two topics, namely AI for meta-optics and meta-optics for AI. The former describes how to apply AI to the research of meta-optics for design, simulation, optical information analysis, and application. The latter reports the development of the optical Al system and computation via meta-optics. This review will also provide an in-depth discussion of the challenges of this interdisciplinary field and indicate future directions. We expect that this review will inspire researchers in these fields and benefit the next generation of intelligent optical device design.
Topics: Artificial Intelligence; Optics and Photonics
PubMed: 35750326
DOI: 10.1021/acs.chemrev.2c00012 -
Molecules (Basel, Switzerland) Jul 2019In recent years, the development of metamaterials and metasurfaces has drawn great attention, enabling many important practical applications. Focusing and lensing... (Review)
Review
In recent years, the development of metamaterials and metasurfaces has drawn great attention, enabling many important practical applications. Focusing and lensing components are of extreme importance because of their significant potential practical applications in biological imaging, display, and nanolithography fabrication. Metafocusing devices using ultrathin structures (also known as metasurfaces) with superlensing performance are key building blocks for developing integrated optical components with ultrasmall dimensions. In this article, we review the metamaterial superlensing devices working in transmission mode from the perfect lens to two-dimensional metasurfaces and present their working principles. Then we summarize important practical applications of metasurfaces, such as plasmonic lithography, holography, and imaging. Different typical designs and their focusing performance are also discussed in detail.
Topics: Lenses; Nanotechnology; Optics and Photonics; Silver; Surface Properties
PubMed: 31277470
DOI: 10.3390/molecules24132460 -
Sensors (Basel, Switzerland) Jan 2022In recent years, the biosensor research community has made rapid progress in the development of nanostructured materials capable of amplifying the interaction between... (Review)
Review
In recent years, the biosensor research community has made rapid progress in the development of nanostructured materials capable of amplifying the interaction between light and biological matter. A common objective is to concentrate the electromagnetic energy associated with light into nanometer-scale volumes that, in many cases, can extend below the conventional Abbé diffraction limit. Dating back to the first application of surface plasmon resonance (SPR) for label-free detection of biomolecular interactions, resonant optical structures, including waveguides, ring resonators, and photonic crystals, have proven to be effective conduits for a wide range of optical enhancement effects that include enhanced excitation of photon emitters (such as quantum dots, organic dyes, and fluorescent proteins), enhanced extraction from photon emitters, enhanced optical absorption, and enhanced optical scattering (such as from Raman-scatterers and nanoparticles). The application of photonic metamaterials as a means for enhancing contrast in microscopy is a recent technological development. Through their ability to generate surface-localized and resonantly enhanced electromagnetic fields, photonic metamaterials are an effective surface for magnifying absorption, photon emission, and scattering associated with biological materials while an imaging system records spatial and temporal patterns. By replacing the conventional glass microscope slide with a photonic metamaterial, new forms of contrast and enhanced signal-to-noise are obtained for applications that include cancer diagnostics, infectious disease diagnostics, cell membrane imaging, biomolecular interaction analysis, and drug discovery. This paper will review the current state of the art in which photonic metamaterial surfaces are utilized in the context of microscopy.
Topics: Biosensing Techniques; Microscopy; Optics and Photonics; Photons; Surface Plasmon Resonance
PubMed: 35161831
DOI: 10.3390/s22031086 -
Nature Communications Jun 2022When pursuing sustainable approaches to fabricate photonic structures, nature can be used as a source of inspiration for both the nanoarchitecture and the constituent...
When pursuing sustainable approaches to fabricate photonic structures, nature can be used as a source of inspiration for both the nanoarchitecture and the constituent materials. Although several biomaterials have been promised as suitable candidates for photonic materials and pigments, their fabrication processes have been limited to the small to medium-scale production of films. Here, by employing a substrate-free process, structurally coloured microparticles are produced via the confined self-assembly of a cholesteric cellulose nanocrystal (CNC) suspension within emulsified microdroplets. Upon drying, the droplets undergo multiple buckling events, which allow for greater contraction of the nanostructure than predicted for a spherical geometry. This buckling, combined with a solvent or thermal post-treatment, enables the production of dispersions of vibrant red, green, and blue cellulose photonic pigments. The hierarchical structure of these pigments enables the deposition of coatings with angular independent colour, offering a consistent visual appearance across a wide range of viewing angles.
Topics: Cellulose; Nanoparticles; Nanostructures; Optics and Photonics; Photons
PubMed: 35697688
DOI: 10.1038/s41467-022-31079-9 -
Chemical Reviews Feb 2022Progress in optical manipulation has stimulated remarkable advances in a wide range of fields, including materials science, robotics, medical engineering, and... (Review)
Review
Progress in optical manipulation has stimulated remarkable advances in a wide range of fields, including materials science, robotics, medical engineering, and nanotechnology. This Review focuses on an emerging class of optical manipulation techniques, termed heat-mediated optical manipulation. In comparison to conventional optical tweezers that rely on a tightly focused laser beam to trap objects, heat-mediated optical manipulation techniques exploit tailorable optothermo-matter interactions and rich mass transport dynamics to enable versatile control of matter of various compositions, shapes, and sizes. In addition to conventional tweezing, more distinct manipulation modes, including optothermal pulling, nudging, rotating, swimming, oscillating, and walking, have been demonstrated to enhance the functionalities using simple and low-power optics. We start with an introduction to basic physics involved in heat-mediated optical manipulation, highlighting major working mechanisms underpinning a variety of manipulation techniques. Next, we categorize the heat-mediated optical manipulation techniques based on different working mechanisms and discuss working modes, capabilities, and applications for each technique. We conclude this Review with our outlook on current challenges and future opportunities in this rapidly evolving field of heat-mediated optical manipulation.
Topics: Hot Temperature; Light; Nanotechnology; Optical Tweezers; Optics and Photonics
PubMed: 34797041
DOI: 10.1021/acs.chemrev.1c00626 -
Lasers in Surgery and Medicine Aug 2021This article reviews deep learning applications in biomedical optics with a particular emphasis on image formation. The review is organized by imaging domains within... (Review)
Review
This article reviews deep learning applications in biomedical optics with a particular emphasis on image formation. The review is organized by imaging domains within biomedical optics and includes microscopy, fluorescence lifetime imaging, in vivo microscopy, widefield endoscopy, optical coherence tomography, photoacoustic imaging, diffuse tomography, and functional optical brain imaging. For each of these domains, we summarize how deep learning has been applied and highlight methods by which deep learning can enable new capabilities for optics in medicine. Challenges and opportunities to improve translation and adoption of deep learning in biomedical optics are also summarized. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
Topics: Deep Learning; Microscopy; Optical Imaging; Optics and Photonics; Tomography, Optical Coherence
PubMed: 34015146
DOI: 10.1002/lsm.23414 -
Biosensors Dec 2022Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and... (Review)
Review
Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniaturization, high scalability, and low-cost mass production. Sensitive, specific detection with silicon photonic sensors is afforded through biofunctionalization of the sensor surface; consequently, this functionalization chemistry is inextricably linked to sensor performance. In this review, we first highlight the biofunctionalization needs for SiP biosensors, including sensitivity, specificity, cost, shelf-stability, and replicability and establish a set of performance criteria. We then benchmark biofunctionalization strategies for SiP biosensors against these criteria, organizing the review around three key aspects: bioreceptor selection, immobilization strategies, and patterning techniques. First, we evaluate bioreceptors, including antibodies, aptamers, nucleic acid probes, molecularly imprinted polymers, peptides, glycans, and lectins. We then compare adsorption, bioaffinity, and covalent chemistries for immobilizing bioreceptors on SiP surfaces. Finally, we compare biopatterning techniques for spatially controlling and multiplexing the biofunctionalization of SiP sensors, including microcontact printing, pin- and pipette-based spotting, microfluidic patterning in channels, inkjet printing, and microfluidic probes.
Topics: Silicon; Optics and Photonics; Antibodies; Lectins; Biosensing Techniques
PubMed: 36671887
DOI: 10.3390/bios13010053 -
Cytometry. Part a : the Journal of the... Aug 2019
Topics: Color; Flow Cytometry; Humans; Optics and Photonics; Spectrum Analysis
PubMed: 31038271
DOI: 10.1002/cyto.a.23779