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Pigment Cell & Melanoma Research Jul 2022The skin acts as a barrier to environmental insults and provides many vital functions. One of these is to shield DNA from harmful ultraviolet radiation, which is...
The skin acts as a barrier to environmental insults and provides many vital functions. One of these is to shield DNA from harmful ultraviolet radiation, which is achieved by skin pigmentation arising as melanin is produced and dispersed within the epidermal layer. This is a crucial defence against DNA damage, photo-ageing and skin cancer. The mechanisms and regulation of melanogenesis and melanin transfer involve extensive crosstalk between melanocytes and keratinocytes in the epidermis, as well as fibroblasts in the dermal layer. Although the predominant mechanism of melanin transfer continues to be debated and several plausible models have been proposed, we and others previously provided evidence for a coupled exo/phagocytosis model. Herein, we performed histology and immunohistochemistry analyses and demonstrated that a newly developed full-thickness three-dimensional reconstructed human pigmented skin model and an epidermis-only model exhibit dispersed pigment throughout keratinocytes in the epidermis. Transmission electron microscopy revealed melanocores between melanocytes and keratinocytes, suggesting that melanin is transferred through coupled exocytosis/phagocytosis of the melanosome core, or melanocore, similar to our previous observations in human skin biopsies. We, therefore, present evidence that our in vitro models of pigmented human skin show epidermal pigmentation comparable to human skin. These findings have a high value for studies of skin pigmentation mechanisms and pigmentary disorders, whilst reducing the reliance on animal models and human skin biopsies.
Topics: Animals; Epidermis; Humans; Keratinocytes; Melanins; Melanocytes; Melanosomes; Pigmentation; Skin; Skin Pigmentation; Ultraviolet Rays
PubMed: 35325505
DOI: 10.1111/pcmr.13039 -
Current Biology : CB Nov 2020Across kingdoms, organisms ameliorate UV stress by increasing UV-absorbing pigmentation. Rapid ozone degradation during the 20 century resulted in elevated UV incidence,...
Across kingdoms, organisms ameliorate UV stress by increasing UV-absorbing pigmentation. Rapid ozone degradation during the 20 century resulted in elevated UV incidence, but pigmentation responses to this aspect of global change have yet to be demonstrated. In flowering plants, UV exposure favors larger areas of UV-absorbing pigmentation on petals, which protects pollen from UV-damage. Pigmentation also affects floral thermoregulation, suggesting climate warming may additionally impact pigmentation. We used 1,238 herbarium specimens collected from 1941 to 2017 to test whether change in UV floral pigmentation was associated with altered ozone and temperature in 42 species spanning three continents. We tested three predictions: first, UV-absorbing pigmentation will increase temporally and be correlated with reduced ozone (higher UV) when accounting for effects of temperature; second, taxa that experienced larger ozone declines will display larger increases in pigmentation; and third, taxa with anthers exposed to ambient UV will respond more strongly than those with anthers protected by petals. Globally, the extent of petal UV pigmentation increased significantly across taxa by ∼2% per year. However, temporal change was species specific-increasing in some taxa but declining in others. Species with exposed anthers experiencing larger declines in ozone displayed more dramatic pigmentation increases. For taxa with anthers enclosed within petals, pigmentation declined with increases in temperature, supporting a thermoregulatory role of UV pigmentation. Results document a rapid phenotypic response of floral pigmentation to anthropogenic climatic change, suggesting that global change may alter pollination through its impact on floral color, with repercussions for plant reproductive fitness.
Topics: Acclimatization; Flowers; Genetic Fitness; Global Warming; Hot Temperature; Ozone; Pigmentation; Plants; Pollination; Ultraviolet Rays
PubMed: 32946752
DOI: 10.1016/j.cub.2020.08.077 -
Australian Journal of General Practice Oct 2023
Topics: Humans; Skin Pigmentation; Skin
PubMed: 37788690
DOI: 10.31128/AJGP-10-23-1234e -
Proceedings. Biological Sciences Aug 2022Organisms living on the seafloor are subject to encrustations by a wide variety of animals, plants and microbes. Sea urchins, however, thwart this covering. Despite...
Organisms living on the seafloor are subject to encrustations by a wide variety of animals, plants and microbes. Sea urchins, however, thwart this covering. Despite having a sophisticated immune system, there is no clear molecular mechanism that allows sea urchins to remain free of epibiotic microorganisms. Here, we test the hypothesis that pigmentation biosynthesis in sea urchin spines influences their interactions with microbes using CRISPR/Cas9. We report three primary findings. First, the microbiome of sea urchin spines is species-specific and much of this community is lost in captivity. Second, different colour morphs associate with bacterial communities that are similar in taxonomic composition, diversity and evenness. Lastly, loss of the pigmentation biosynthesis genes polyketide synthase and flavin-dependent monooxygenase induces a shift in which bacterial taxa colonize sea urchin spines. Therefore, our results are consistent with the hypothesis that host pigmentation biosynthesis can, but may not always, influence the microbiome in sea urchin spines.
Topics: Animals; Bacteria; Microbiota; Pigmentation; Polyketide Synthases; Sea Urchins
PubMed: 35975446
DOI: 10.1098/rspb.2022.1088 -
Proceedings. Biological Sciences Feb 2021Developmental plasticity is ubiquitous in natural populations, but the underlying causes and fitness consequences are poorly understood. For consumers, nutritional...
Developmental plasticity is ubiquitous in natural populations, but the underlying causes and fitness consequences are poorly understood. For consumers, nutritional variation of juvenile diets is probably associated with plasticity in developmental rates, but little is known about how diet quality can affect phenotypic trajectories in ways that might influence survival to maturity and lifetime reproductive output. Here, we tested how the diet quality of a freshwater detritivorous isopod (), in terms of elemental ratios of diet (i.e. carbon : nitrogen : phosphorus; C : N : P), can affect (i) developmental rates of body size and pigmentation and (ii) variation in juvenile survival. We reared 1047 individuals, in a full-sib split-family design (29 families), on either a high- (low C : P, C : N) or low-quality (high C : P, C : N) diet, and quantified developmental trajectories of body size and pigmentation for every individual over 12 weeks. Our diet contrast caused strong divergence in the developmental rates of pigmentation but not growth, culminating in a distribution of adult pigmentation spanning the broad range of phenotypes observed both within and among natural populations. Under low-quality diet, we found highest survival at intermediate growth and pigmentation rates. By contrast, survival under high-quality diet survival increased continuously with pigmentation rate, with longest lifespans at intermediate growth rates and high pigmentation rates. Building on previous work which suggests that visual predation mediates the evolution of cryptic pigmentation in , our study shows how diet quality and composition can generate substantial phenotypic variation by affecting rates of growth and pigmentation during development in the absence of predation.
Topics: Animals; Diet; Fresh Water; Isopoda; Phenotype; Pigmentation
PubMed: 33593189
DOI: 10.1098/rspb.2020.3136 -
Acta Ophthalmologica Jun 2022To compare the refractive outcome and residual accommodation with respect to various degrees of iris and skin pigmentation in hypermetropic children using 2 drops of... (Randomized Controlled Trial)
Randomized Controlled Trial
Eye colour and skin pigmentation as significant factors for refractive outcome and residual accommodation in hypermetropic children: a randomized clinical trial using cyclopentolate 1% and tropicamide 1.
PURPOSE
To compare the refractive outcome and residual accommodation with respect to various degrees of iris and skin pigmentation in hypermetropic children using 2 drops of cyclopentolate 1% (C + C) or 1 drop of cyclopentolate 1% and 1 drop of tropicamide 1% (C + T).
METHODS
Two hundred fifty-one hypermetropic children were classified according to iris and skin pigmentation (light, medium, dark) and received randomized and double-blind C + C or C + T. Refractive error (spherical equivalent, SEQ) was determined using the Retinomax-K + 3. In 204 subjects, residual accommodation (RA) was determined using the PlusoptiX PowerRefractor.
RESULTS
A linear mixed model with a light-irided and light skin-pigmented reference group receiving C + T (mean SEQ +3.10 ± 1.87D) indicated significant less hypermetropia in subjects with a dark iris having a medium- and dark-pigmented skin in C + T, -1.02 ± 0.29 (-1.59/-0.45) and -1.53 ± 0.30 (-2.10/-0.95); and in subjects having a light-, medium- and dark-pigmented skin in C + C, -0.74 ± 0.34 (-1.41/-0.06), -1.26 ± 0.30 (-1.85/-0.66) and -1.84 ± 0.30 (-2.42/-1.26). Similar findings were present for RA. Our model with a light-irided and light skin-pigmented reference group receiving C + T (mean RA +0.84 ± 0.61D) indicated significantly higher RA in dark-irided subjects with medium- and dark-pigmented skin in C + T, +1.05 ± 0.19 (+0.67/+1.43) and +1.35 ± 0.20 (+0.9/+1.74), and in C + C, +1.13 ± 0.21 (+0.71/+1.55) and +1.90 ± 0.19 (+1.51/+2.28).
CONCLUSIONS
We found solid evidence that skin pigmentation rather than iris pigmentation is the decisive factor for effectiveness of cycloplegics. Awareness of the limitations of cycloplegic regimens in dark-irided/pigmented children is needed. Our study showed that cyclopentolate 1% combined with tropicamide 1% provides more accurate refractive outcomes both statistically and clinically integrating the factor skin pigmentation for dark-irided subjects.
Topics: Child; Cyclopentolate; Eye Color; Humans; Mydriatics; Ophthalmic Solutions; Skin Pigmentation; Tropicamide
PubMed: 34672100
DOI: 10.1111/aos.15016 -
Skin Research and Technology : Official... Jul 2023The difference in skin pigmentation induced by blue light between melasma patients and healthy people has not been reported. This study aimed to explore the impact of... (Observational Study)
Observational Study
BACKGROUND
The difference in skin pigmentation induced by blue light between melasma patients and healthy people has not been reported. This study aimed to explore the impact of different doses of blue light irradiation on the pigmentation of the skin of non-exposed areas in female melasma patients with III-IV-type skin and healthy women.
MATERIALS AND METHODS
This observational study enrolled patients with melasma and healthy people at the First Affiliated Hospital of Kunming Medical University between January and April 2021. The outcomes were the degree of pigmentation, ΔL*, and ΔITA* values.
RESULTS
Forty-two (21/group) participants were enrolled. After irradiation with different doses of blue light, different degrees of pigmentation could be observed in the irradiated area of the skin of female melasma patients and healthy women. The △L* and △ITA* values in the irradiated area of the skin of healthy women were higher than in female melasma patients after blue light irradiation at 20 J/cm (p < 0.05). There were no significant differences in the pigmentation scores, △L* values, and △ITA* values in the irradiated areas of skin at different time points after irradiation with the other doses of blue light (p > 0.05).
CONCLUSION
Blue light at 20 J/cm induced a smaller change in pigmentation in melasma patients than in healthy women, but the effect of blue light at 40-80 J/cm was similar.
Topics: Humans; Female; Skin Pigmentation; Light; Skin; Melanosis
PubMed: 37522494
DOI: 10.1111/srt.13401 -
Current Opinion in Genetics &... Aug 2019The genetic basis of morphological variation, both within and between species, provides a major topic in evolutionary biology. Teleost fish produce most elaborate color... (Review)
Review
The genetic basis of morphological variation, both within and between species, provides a major topic in evolutionary biology. Teleost fish produce most elaborate color patterns, and among the more than 20000 species a number have been chosen for more detailed analyses because they are suitable to study particular aspects of color pattern evolution. In several fish species, color variants and pattern variants have been collected, transcriptome analyses have been carried out, and the recent advent of gene editing tools, such as CRISPR/Cas9, has allowed the production of mutants. Covering mostly the literature from the last three years, we discuss the cellular basis of coloration and the identification of loci involved in color pattern differences between sister species in cichlids and Danio species, in which cis-regulatory changes seem to prevail.
Topics: Animals; CRISPR-Cas Systems; Cichlids; Evolution, Molecular; Gene Expression Regulation, Developmental; Pigmentation; Transcriptome; Zebrafish
PubMed: 31421397
DOI: 10.1016/j.gde.2019.07.002 -
The Yale Journal of Biology and Medicine Mar 2020The relationship between skin color and skin cancer is well established: the less melanin in one's skin the greater the risk for developing skin cancer. This review is... (Review)
Review
The relationship between skin color and skin cancer is well established: the less melanin in one's skin the greater the risk for developing skin cancer. This review is in two parts. First, we summarize the current understanding of the cutaneous pigmentary system and trace melanin from its synthesis in the pigment cell melanosomes through its transfer to keratinocytes. We also present new methods for reducing melanin content in hyper-pigmented areas of skin such as solar lentigenes, melasma, and post-inflammatory hyperpigmentation. Second, we present evidence that at least one mechanism for the development of metastatic melanoma and other solid tumors is fusion and hybridization of leucocytes such as macrophages with primary tumor cells. In this scenario, hybrid cells express both the chemotactic motility of the leucocyte and the de-regulated cell division of the tumor cell, causing the cells to migrate a deadly journey to lymph nodes, distant organs, and tissues.
Topics: Humans; Hyperpigmentation; Melanins; Skin Neoplasms; Skin Pigmentation
PubMed: 32226338
DOI: No ID Found -
ELife Jan 2022Variation in floral displays, both between and within species, has been long known to be shaped by the mutualistic interactions that plants establish with their...
Variation in floral displays, both between and within species, has been long known to be shaped by the mutualistic interactions that plants establish with their pollinators. However, increasing evidence suggests that abiotic selection pressures influence floral diversity as well. Here, we analyse the genetic and environmental factors that underlie patterns of floral pigmentation in wild sunflowers. While sunflower inflorescences appear invariably yellow to the human eye, they display extreme diversity for patterns of ultraviolet pigmentation, which are visible to most pollinators. We show that this diversity is largely controlled by -regulatory variation affecting a single MYB transcription factor, HaMYB111, through accumulation of ultraviolet (UV)-absorbing flavonol glycosides in ligules (the 'petals' of sunflower inflorescences). Different patterns of ultraviolet pigments in flowers are strongly correlated with pollinator preferences. Furthermore, variation for floral ultraviolet patterns is associated with environmental variables, especially relative humidity, across populations of wild sunflowers. Ligules with larger ultraviolet patterns, which are found in drier environments, show increased resistance to desiccation, suggesting a role in reducing water loss. The dual role of floral UV patterns in pollinator attraction and abiotic response reveals the complex adaptive balance underlying the evolution of floral traits.
Topics: Adaptation, Physiological; Flavonols; Helianthus; Phenotype; Pigmentation; Pollination; Ultraviolet Rays
PubMed: 35040432
DOI: 10.7554/eLife.72072