-
Ecotoxicology and Environmental Safety Oct 2023Accumulating evidence shows widespread contamination of water sources and food with microplastics. Although the liver is one of the main sites of bioaccumulation within...
Accumulating evidence shows widespread contamination of water sources and food with microplastics. Although the liver is one of the main sites of bioaccumulation within the human body, it is still unclear whether microplastics produce damaging effects. In particular, the hepatic consequences of ingesting polyethylene (PE) microplastics in mammals are unknown. In this study, female mice were fed with food contaminated with 36 and 116 µm diameter PE microbeads at a dosage of 100 µg/g of food for 6 and 9 weeks. Mice were exposed to each type of microbead, or co-exposed to the 2 types of microbeads. Mouse liver showed altered levels of genes involved in uptake, synthesis, and β-oxidation of fatty acids. Ingestion of PE microbeads disturbed the detoxification response, promoted oxidative imbalance, increased inflammatory foci and cytokine expression, and enhanced proliferation in liver. Since relative expression of the hepatic stellate cell marker Pdgfa and collagen deposition were increased following PE exposure, we assessed the effect of PE ingestion in a mouse model of CCl-induced fibrosis and showed that PE dietary exposure exacerbated liver fibrogenesis. These findings provide the first demonstration of the adverse hepatic effects of PE ingestion in mammals and highlight the need for further health risk assessment in humans.
Topics: Humans; Female; Animals; Mice; Polyethylene; Microplastics; Plastics; Liver; Drug-Related Side Effects and Adverse Reactions; Fibrosis; Mammals
PubMed: 37651791
DOI: 10.1016/j.ecoenv.2023.115417 -
Chemosphere Oct 2023Bis(2-ethylhexyl) phthalate (DEHP) transfer from a polyvinyl chloride (PVC) sheet to 9 kinds of particles, namely, polyethylene particles (1-10, 45-53, 90-106 μm),...
Bis(2-ethylhexyl) phthalate (DEHP) transfer from a polyvinyl chloride (PVC) sheet to 9 kinds of particles, namely, polyethylene particles (1-10, 45-53, 90-106 μm), soda lime glass particles (1-38, 45-53, 90-106 μm), black forest soil, carbon black, and cotton linter, for the particle weights of 0.3, 1, 3, and 12 mg/cm, were determined for 1, 3, 7, and 14 days using a passive flux sampler (PFS), as well as standard dust. Transfer amounts to small polyethylene particles (1-10 μm), black forest soil, and carbon black were large (8.5, 16, and 48 μg/mg-particle, respectively, for 0.3 mg/cm for 14 days) and were similar to standard house dust (35 μg/mg-particle). On the other hand, transfer amount to large polyethylene particles (0.056-0.12 μg/mg-particle), soda lime glass (0.18-0.31 μg/mg-particle), and cotton linter (0.42-0.78 μg/mg-particle) were much lower. The DEHP transfer amount to the particles was proportional to the surface area of the particles, but not associated with the organic content. The DEHP transfer amount per surface area to small polyethylene particles was larger than that of other particles, suggesting the contribution of absorption into the polyethylene particle. However, for the larger polyethylene particles with different manufacturing process that may have different crystallinity, the contribution of absorption was small. The amount of DEHP transferred to soda lime glass did not differ from 1 to 14 days, suggesting that an adsorption equilibrium was reached after 1 day. The estimated value of particle/gas partition coefficients of DEHP, K, of small polyethylene, black forest soil and carbon black were much higher (3.6, 7.1, and 18 m/mg, respectively) than those of large polyethylene and soda lime glass particles (0.028-0.11 m/mg).
Topics: Diethylhexyl Phthalate; Polyvinyl Chloride; Soot; Phthalic Acids; Dust; Polyethylene
PubMed: 37433409
DOI: 10.1016/j.chemosphere.2023.139438 -
STAR Protocols Dec 2023Depolymerization and upcycling are promising approaches to managing plastic waste. However, quantitative measurements of reaction rates and analyses of complex product...
Depolymerization and upcycling are promising approaches to managing plastic waste. However, quantitative measurements of reaction rates and analyses of complex product mixtures arising from depolymerization of polyolefins constitute significant challenges in this emerging field. Here, we detail techniques for recovery and analysis of products arising from batch depolymerization of polyethylene. We also describe quantitative analyses of reaction rates and products selectivity. This protocol can be extended to depolymerization of other plastics and characterization of other product mixtures including long-chain olefins. For complete details on the use and execution of this protocol, please refer to Sun et al..
Topics: Polyethylene; Alkenes
PubMed: 37729056
DOI: 10.1016/j.xpro.2023.102575 -
Environmental Science and Pollution... Dec 2023The water quality implications of transferring stormwater through pipes composed of concrete (new and used), polyvinyl chloride (PVC), galvanized corrugated steel (GCS),... (Review)
Review
The water quality implications of transferring stormwater through pipes composed of concrete (new and used), polyvinyl chloride (PVC), galvanized corrugated steel (GCS), high-density polyethylene (HDPE), and pipes subjected to cured in place pipe (CIPP) and spray in place pipe (SIPP) trenchless repair technologies on stormwater quality are reviewed. Studies involve either the use of flowing water or an immersion experimental design, with data showing contact with pipe materials can affect stormwater quality parameters including pH, electrical conductivity (EC), and concentrations of minerals, metals, and organic constituents, e.g. styrene. 'In-transport' changes in pH (1-3 units), EC (2-3-fold), bicarbonate (3-44-fold), and calcium (2-17-fold) in stormwaters were reported following exposure to concrete pipes. Differences between the use of synthetic and field-collected stormwater were identified, e.g. turbidity levels in field-collected stormwater reduced on passage through all pipe types, compared to synthetic water where levels of turbidity on exposure to concrete and cement-based SIPP increased slightly. Transfer through PVC and HDPE pipes had minimal effects on physicochemical parameters, whereas exposure to galvanized corrugated steel pipes led to increases in EC, Zn, and Pb. Though limited data was available, the use of CIPP repairs and associated waste condensate generated during thermal curing and/or incomplete curing of resins was identified to release organic contaminants of concerns (e.g. styrene, vinylic monomers, dibutyl phthalate (DBP), diethyl phthalate (DEP), and benzaldehyde). The implications of findings for both future research and stakeholders with responsibility for reducing diffuse pollution loads to receiving waters are considered.
Topics: Polyethylene; Water Quality; Styrene; Dibutyl Phthalate; Steel
PubMed: 37924396
DOI: 10.1007/s11356-023-30508-6 -
Journal of Nanobiotechnology Oct 2023The wear particle-induced dissolution of bone around implants is a significant pathological factor in aseptic loosening, and controlling prosthetic aseptic loosening...
PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis.
The wear particle-induced dissolution of bone around implants is a significant pathological factor in aseptic loosening, and controlling prosthetic aseptic loosening holds crucial social significance. While human umbilical cord mesenchymal stem cell-derived exosomes (HucMSCs-Exos, Exos) have been found to effectively promote osteogenesis and angiogenesis, their role in periprosthetic osteolysis remains unexplored. To enhance their in vivo application, we engineered HucMSCs-Exos-encapsulated poly lactic-co-glycolic acid (PLGA) nanoparticles (PLGA-Exos). In our study, we demonstrate that PLGA-Exos stimulate osteogenic differentiation while inhibiting the generation of reactive oxygen species (ROS) and subsequent osteoclast differentiation in vitro. In vivo imaging revealed that PLGA-Exos released exosomes slowly and maintained a therapeutic concentration. Our in vivo experiments demonstrated that PLGA-Exos effectively suppressed osteolysis induced by polyethylene particles. These findings suggest that PLGA-Exos hold potential as a therapeutic approach for the prevention and treatment of periprosthetic osteolysis. Furthermore, they provide novel insights for the clinical management of osteolysis.
Topics: Humans; Osteogenesis; Osteolysis; Polyethylene; Glycols; Mesenchymal Stem Cells; Exosomes; Nanoparticles; Umbilical Cord
PubMed: 37904168
DOI: 10.1186/s12951-023-02177-7 -
Environment International Mar 2024Microplastics (MPs) are inevitably oxidized in the environment, and their potential toxicity to organisms has attracted wide attention. However, the neurotoxicity and...
Microplastics (MPs) are inevitably oxidized in the environment, and their potential toxicity to organisms has attracted wide attention. However, the neurotoxicity and mechanism of oxidized polyethylene (Ox-PE) MPs to organisms remain unclear. Herein, we prepared oxidized low-density polyethylene (Ox-LDPE) and established a model of MPs exposure by continuously orally gavage of C57BL/6 J mice with LDPE-MPs/Ox-LDPE-MPs for 28 days with or without oral administration of Lactobacillus plantarum DP189 and galactooligosaccharides (DP189&GOS). The experimental results indicated that LDPE-MPs or Ox-LDPE-MPs caused several adverse effects in mice, mainly manifested by behavioral changes, disruption of the intestinal and blood-brain barrier (BBB), and simultaneous oxidative stress, inflammatory reactions, and pathological damage in the brain and intestines. Brain transcriptomic analysis revealed that the cholinergic synaptic signaling pathways, which affect cognitive function, were significantly disrupted after exposure to LDPE-MPs or Ox-LDPE-MPs. Real-time quantitative polymerase chain reaction and Western Blotting results further demonstrated that the critical genes (Slc5a7, Chat and Slc18a3) and proteins (Chat and Slc18a3) in the cholinergic synaptic signaling pathway were significantly down-regulated after exposure to LDPE-MPs or Ox-LDPE-MPs. These alterations lead to reduced acetylcholine concentration, which causes cognitive dysfunction in mice. Importantly, the DP189&GOS interventions effectively mitigated the MPs-induced cognitive dysfunction and intestinal microbiota alteration, improved intestinal and BBB integrity, attenuated the oxidative stress and inflammatory response, and also saw a rebound in the release of acetylcholine. These results indicated that LDPE-MPs and Ox-LDPE-MPs exert neurotoxic effects on mice by inducing oxidative stress, inflammatory responses, and dysregulation of cholinergic signaling pathways in the mouse brain. That probiotic supplementation is effective in attenuating MPs-induced neurotoxicity in mice. Overall, this study reveals the potential mechanisms of neurotoxicity of LDPE-MPs and Ox-LDPE-MPs on mice and their improvement measures, necessary to assess the potential risks of plastic contaminants to human health.
Topics: Humans; Animals; Mice; Polyethylene; Mice, Inbred C57BL; Microplastics; Plastics; Brain-Gut Axis; Acetylcholine; Cholinergic Agents
PubMed: 38484610
DOI: 10.1016/j.envint.2024.108523 -
Chemosphere Dec 2023This study investigated the toxicity of polyethylene microplastics (MPs; <0.02 mm) and CuSO4, alone and in combination, on the freshwater crayfish Pontastacus...
Single and combined effects of CuSO and polyethylene microplastics on biochemical endpoints and physiological impacts on the narrow-clawed crayfish Pontastacusleptodactylus.
This study investigated the toxicity of polyethylene microplastics (MPs; <0.02 mm) and CuSO4, alone and in combination, on the freshwater crayfish Pontastacus leptodactylus. In this study, the crayfish were exposed to PE-MPs (0.0, 0.5, and 1 mg L) and CuSO4·5H2O (0.0, 0.5, and 1 mg L) for a period of 28 days. Next, multi-biomarkers, including biochemical, immunological, and oxidative stress indicators were analyzed. Results showed that co-exposure to PE-MPs and CuSO4 resulted in increased aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and decreased alkaline phosphatase (ALP), butyrylcholinesterase (BChE), and gamma-glutamyl-transferase (GGT). Triglycerides, cholesterol, glucose, and albumin content also increased. Although no significant change was observed in lysozyme and phenoloxidase activities in crayfish co-exposed to 0.5 mg L MPs and 0.5 mg L CuSO4, their activities were significantly decreased in other experimental groups. Oxidative stress parameters in hepatopancreas indicated increased superoxide dismutase (SOD), glutathione peroxidase (GPx), and in malondialdehyde (MDA) levels, but decreased catalase (CAT), glucose 6-phosphate dehydrogenase (G6PDH), and cellular total antioxidant (TAC). Results showed that the sub-chronic toxicity of CuSO4 was confirmed. The study confirmed the toxicity of CuSO4 and found that higher concentrations led to more severe effects. Co-exposure to PE-MPs and CuSO4 primarily compromised the endpoints, showing increased toxicity when both pollutants were present in higher concentrations. The activities of POX, LYZ, ALP, GGT, LDH, and CAT were suppressed by both CuSO4 and MPs. However, a synergistic increase was observed in other measured biomarkers in crayfish co-exposed to CuSO4 and MPs.
Topics: Animals; Microplastics; Polyethylene; Plastics; Astacoidea; Butyrylcholinesterase; Antioxidants; Oxidative Stress; Glucose; Biomarkers
PubMed: 37865200
DOI: 10.1016/j.chemosphere.2023.140478 -
BMC Biotechnology Jul 2023Plastic pollution is a major global concern to the health and wellbeing of all terrestrial and marine life. However, no sustainable method for waste management is...
Plastic pollution is a major global concern to the health and wellbeing of all terrestrial and marine life. However, no sustainable method for waste management is currently viable. This study addresses the optimisation of microbial enzymatic polyethylene oxidation through rational engineering of laccases with carbohydrate-binding module (CBM) domains. An explorative bioinformatic approach was taken for high-throughput screening of candidate laccases and CBM domains, representing an exemplar workflow for future engineering research. Molecular docking simulated polyethylene binding whilst a deep-learning algorithm predicted catalytic activity. Protein properties were examined to interpret the mechanisms behind laccase-polyethylene binding. The incorporation of flexible GGGGS(x3) hinges were found to improve putative polyethylene binding of laccases. Whilst CBM1 family domains were predicted to bind polyethylene, they were suggested to detriment laccase-polyethylene associations. In contrast, CBM2 domains reported improved polyethylene binding and may thus optimise laccase oxidation. Interactions between CBM domains, linkers, and polyethylene hydrocarbons were heavily reliant on hydrophobicity. Preliminary polyethylene oxidation is considered a necessity for consequent microbial uptake and assimilation. However, slow oxidation and depolymerisation rates inhibit the large-scale industrial implementation of bioremediation within waste management systems. The optimised polyethylene oxidation of CBM2-engineered laccases represents a significant advancement towards a sustainable method of complete plastic breakdown. Results of this study offer a rapid, accessible workflow for further research into exoenzyme optimisation whilst elucidating mechanisms behind the laccase-polyethylene interaction.
Topics: Laccase; Polyethylene; Molecular Docking Simulation; Oxidation-Reduction; Carbohydrates
PubMed: 37415113
DOI: 10.1186/s12896-023-00787-5 -
Knee Surgery, Sports Traumatology,... Oct 2021The Oxford unicompartmental knee replacement (UKR) has a fully congruent mobile bearing to minimise wear. However, with younger higher demand patients, wear remains a...
PURPOSE
The Oxford unicompartmental knee replacement (UKR) has a fully congruent mobile bearing to minimise wear. However, with younger higher demand patients, wear remains a concern. The aim of this study was to quantify the wear rate of Phase 3 Oxford UKR bearings over the course of 5 years and to identify the factors that influence it.
METHODS
40 medial Oxford UKRs recruited for a randomised study of cemented and cementless fixation were studied with Radiostereometric analysis (RSA) at 1 week, 3 months, 6 months, 1 year, 2 years, and 5 years post-operatively and bearing thickness was calculated. Penetration, defined as the change in thickness compared to the 1-week measurement, was determined. Creep (early penetration) and wear (late penetration at a constant rate) were calculated. The influence of demographic factors, Oxford Knee Score (OKS), Tegner score, fixation and bearing overhang (determined by RSA) on wear was analysed.
RESULTS
After 6 months the penetration rate was constant, indicating that wear alone was occurring. The wear rate was 0.07 mm/year (SD 0.03). The creep was 0.06 mm with about 95% occurring during the first 3 months. There was no significant relationship between fixation (cemented/cementless), age, component size, OKS and Tegner score with wear rate. Increasing BMI was associated with decreasing wear (p = 0.042). 37/40 bearings overhung the tibia to some extent and 23/40 overhung the tibia medially. An increase in the area of overhang (p = 0.036), amount of medial overhang (p = 0.028) and distance between the bearing and tibial wall (p = 0.019) were associated with increased wear. Bearings that did not overhang (0.06 mm/year) had less wear (p = 0.025) than those that did (0.08 mm/year). There was no relationship (p = 0.6) between the femoral contact area and wear.
CONCLUSION
During the first three to six months after implantation, the bearing becomes 0.06 mm thinner due to creep. The combined wear rate of the upper and lower surfaces of the bearing is constant (0.07 mm/year). The wear is lower if the bearing does not overhang the tibia so surgeons should aim for the bearing to be close to the tibial wall. The orientation of the femoral component does not influence wear.
LEVEL OF EVIDENCE
Retrospective Study, Level III.
Topics: Arthroplasty, Replacement, Knee; Humans; Knee Prosthesis; Osteoarthritis, Knee; Polyethylene; Prosthesis Design; Retrospective Studies
PubMed: 32940731
DOI: 10.1007/s00167-020-06243-7 -
International Journal of Molecular... Jul 2020Priority pollutants such as polyethylene (PE) microplastic, lead (Pb), and cadmium (Cd) have attracted the interest of environmentalists due to their ubiquitous nature...
Priority pollutants such as polyethylene (PE) microplastic, lead (Pb), and cadmium (Cd) have attracted the interest of environmentalists due to their ubiquitous nature and toxicity to all forms of life. In this study, periphytic biofilms (epiphyton and epixylon) were used to bioremediate heavy metals (HMs) and to biodegrade PE under high (120,000 ppm) methane (CH) doses. Both periphytic biofilms were actively involved in methane oxidation, HMs accumulation and PE degradation. Epiphyton and epixylon both completely removed Pb and Cd at concentrations of 2 mg L and 50 mg L, respectively, but only partially removed these HMs at a relatively higher concentration (100 mg L). Treatment containing 12% CH proved to be most effective for biodegradation of PE. A synergistic effect of HMs and PE drastically changed microbial biota and methanotrophic communities. High-throughput 16S rRNA gene sequencing revealed that Cyanobacteria was the most abundant class, followed by Gammaproteobacteria and Alphaproteobacteria in all high-methane-dose treatments. DNA stable-isotope probing was used to label C in a methanotrophic community. A biomarker for methane-oxidizing bacteria, gene sequence of a C-labeled fraction, revealed that was most abundant in all high-methane-dose treatments compared to near atmospheric methane (NAM) treatment, followed by . , , and were also found to be increased by high doses of methane compared to NAM treatment. Overall, Cd had a more determinantal effect on methanotrophic activity than Pb. Epiphyton proved to be more effective than epixylon in HMs removal and PE biodegradation. The findings proved that both epiphyton and epixylon can be used to bioremediate HMs and biodegrade PE as an efficient ecofriendly technique under high methane concentrations.
Topics: Alphaproteobacteria; Biodegradation, Environmental; Biofilms; Cadmium; Gammaproteobacteria; Lead; Methane; Polyethylene
PubMed: 32727088
DOI: 10.3390/ijms21155331