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Nutrients Dec 2020Chronic metabolic acidosis leads to bone-remodelling disorders based on excessive mineral matrix resorption and inhibition of bone formation, but also affects the...
Chronic metabolic acidosis leads to bone-remodelling disorders based on excessive mineral matrix resorption and inhibition of bone formation, but also affects the homeostasis of citrate, which is an essential player in maintaining the acid-base balance and in driving the mineralisation process. This study aimed to investigate the impact of acidosis on the osteogenic properties of bone-forming cells and the effects of citrate supplementation in restoring the osteogenic features impaired by the acidic milieu. For this purpose, human mesenchymal stromal cells were cultured in an osteogenic medium and the extracellular matrix mineralisation was analysed at the micro- and nano-level, both in neutral and acidic conditions and after treatment with calcium citrate and potassium citrate. The acidic milieu significantly decreased the citrate release and hindered the organisation of the extracellular matrix, but the citrate supplementation increased collagen production and, particularly calcium citrate, promoted the mineralisation process. Moreover, the positive effect of citrate supplementation was observed also in the physiological microenvironment. This in vitro study proves that the mineral matrix organisation is influenced by citrate availability in the microenvironment surrounding bone-forming cells, thus providing a biological basis for using citrate-based supplements in the management of bone-remodelling disorders related to chronic low-grade acidosis.
Topics: Acid-Base Equilibrium; Acidosis; Bone Remodeling; Calcium Chelating Agents; Cells, Cultured; Citric Acid; Dietary Supplements; Humans; In Vitro Techniques; Osteogenesis
PubMed: 33317151
DOI: 10.3390/nu12123779 -
Molecules (Basel, Switzerland) Nov 2023We previously enabled a direct insight into the quality of citrate anticoagulant tubes before their intended use for specimen collection by introducing an...
We previously enabled a direct insight into the quality of citrate anticoagulant tubes before their intended use for specimen collection by introducing an easy-to-perform UV spectrometric method for citrate determination on a purified water model. The results revealed differences between the tubes of three producers, Greiner BIO-ONE (A), LT Burnik (B), and BD (C). It became apparent that tubes C contain an additive, which absorbs light in the ultraviolet range and prevents reliable evaluation of citrate anticoagulant concentration with the suggested method. In this research, we re-evaluate the quality of citrate-evacuated blood collection tubes by complementing UV spectrometry with ion chromatography. (1) Comparable results were obtained for tubes B at 220 nm. (2) Citrate concentrations determined with ion chromatography were lower for tubes A and C. Chromatograms reveal additional peaks for both. (3) Influences of heparin on absorption spectra and chromatograms of citrate were studied. Some similarities with the shape of the anticoagulant spectra of tubes A and C were observed, and the lithium heparin peak in chromatograms is close to them, but a confident judgment was not possible. (4) Contamination of anticoagulant solution with potassium, magnesium, and calcium was confirmed for all the brands, and contamination with lithium for B and C.
Topics: Citric Acid; Lithium; Anticoagulants; Heparin; Citrates; Spectrophotometry, Ultraviolet
PubMed: 38067465
DOI: 10.3390/molecules28237735 -
Journal of Feline Medicine and Surgery Jun 2020The aim of this study was to assess the effect of three oral potassium supplements (potassium gluconate tablets [PGT], potassium gluconate granules [PGG] and potassium...
OBJECTIVES
The aim of this study was to assess the effect of three oral potassium supplements (potassium gluconate tablets [PGT], potassium gluconate granules [PGG] and potassium citrate granules [PCG]) on hypokalemia and serum bicarbonate in cats with chronic kidney disease (CKD).
METHODS
Medical records (2006-2016) were retrospectively searched for cats that had been prescribed an oral potassium supplement for management of their CKD-associated hypokalemia. For inclusion, laboratory work had to be available at the time of hypokalemia diagnosis, and at recheck within 1-6 weeks. Treatment response was defined in three ways: any increase in potassium, an increase in potassium to within the normal reference interval, and an increase to >4 mEq/l.
RESULTS
Thirty-seven cats met inclusion criteria (16 PGT, 11 PGG, 10 PCG). Dosing ranged from 0.21 to 1.6 mEq/kg/day for PGT, from 0.25 to 1.48 mEq/kg/day for PGG and from 0.04 to 1.34 mEq/kg/day for PCG. After supplementation, 36/37 cats had an increase in potassium, 34/37 increased to within the reference interval and 24/37 had an increase in potassium to >4 mEq/l. There was a statistically significant difference in serum potassium post-supplementation for all three treatments: PGT ( = 0.0001), PGG ( = 0.001) and PCG ( = 0.002). There was a positive correlation between PGT dose and change in potassium concentration ( = 0.04), but there was no significant correlation for PGG or PCG. In cats that had data available, serum bicarbonate increased >2 mEq/l in 1/6 PGT, 1/6 PGG and 3/4 PCG cats.
CONCLUSIONS AND RELEVANCE
All three potassium supplements were effective in treating hypokalemia secondary to CKD in the majority of cats despite variable dosing. Data were limited to assess the alkalinizing effect and prospective studies are needed.
Topics: Animal Feed; Animals; Bicarbonates; Cat Diseases; Cats; Diet; Dietary Supplements; Female; Hypokalemia; Male; Potassium Citrate; Potassium Compounds; Renal Insufficiency, Chronic; Retrospective Studies
PubMed: 31304869
DOI: 10.1177/1098612X19862084 -
Food Science & Nutrition Sep 2021This study aimed to determine the percentage of reduction of lead and cadmium by chelating agents (potassium tartrate and potassium citrate) in the steps of soaking,...
The effect of chelating agents including potassium tartrate and citrate on the maximum reduction of lead and cadmium during soaking and cooking from some different varieties of rice available in Iran.
This study aimed to determine the percentage of reduction of lead and cadmium by chelating agents (potassium tartrate and potassium citrate) in the steps of soaking, cooking, and simultaneous soaking and cooking in some varieties of rice for the first time. Each chemical experiment was performed in ten replications. Inductively coupled plasma mass spectrometry (Agilent-7700X ICP-MS) was used to assess the complete Cd and Pb content in rice samples acid-digested (500 mg dry-sample, 9 ml HNO: 3 ml HCl). The cooking-only treatment was more successful in terms of lead reduction than the soaking-only treatment in chelating agent-containing solutions (either potassium tartrate or potassium citrate), though it had the same effect on cadmium reduction. Simultaneous soaking and cooking in chelating agents such as potassium tartrate and potassium citrate significantly reduced lead (reduction rate compared to control 99.43% with potassium tartrate and 98.96% with potassium citrate) and cadmium (reduction rate compared to control 95.13% with potassium tartrate and 92.77% with potassium citrate). Potassium tartrate outperforms potassium citrate in terms of lead reduction, but potassium tartrate is equivalent to potassium citrate in terms of cadmium reduction. Up to 200 ppm applicable chelating agents, sensory analysis showed no statistically significant difference between the treatments. In general, rice cookers are advised to use levels up to 200 ppm of citrate or potassium tartrate in combination in the 3-hr rinsing period and then in the 15-min cooking period to reduce the percentage of dangerous heavy metals, especially lead 99%-99.4% and cadmium 92.8%-95.1%.
PubMed: 34532020
DOI: 10.1002/fsn3.2473 -
Life (Basel, Switzerland) Aug 2022Approximately 1 in 20 people develops kidney stones at some point in their life. Although the surgical removal of stones is common, the recurrence rate remains high and...
Approximately 1 in 20 people develops kidney stones at some point in their life. Although the surgical removal of stones is common, the recurrence rate remains high and it is therefore important to prevent the occurrence of kidney stones. We chose (AM), which is a traditional Chinese medicine, to study the prevention of urolithiasis using a model based on our previous screening of traditional Chinese herbs. Wild-type Canton-S adult fruit flies were used in this study. Ethylene glycol (EG, 0.5%) was added to food as a lithogenic agent. The positive control agent (2% potassium citrate (K-citrate)) was then compared with AM (2, 8, and 16 mg/mL). After 21 days, the fruit flies were sacrificed under carbon dioxide narcotization, and the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination to observe calcium oxalate (CaOx) crystallization. Then, the ex vivo dissolution of crystals in the Malpighian tubules was compared between K-citrate and AM. Survival analysis of the EG, K-citrate, and AM groups was also performed. Both 2% K-citrate and AM (16 mg/mL) significantly inhibited EG-induced CaOx crystal formation. Mean lifespan was significantly reduced by the administration of EG, and the results were significantly reversed in the AM (8 and 16 mg/mL) groups. However, AM extract did not directly dissolve CaOx crystals in Malpighian tubules ex vivo. In conclusion, AM extract decreased the ratio of CaOx crystallization in the Malpighian tubules and significantly ameliorated EG-induced reduction of lifespan. AM prevented CaOx crystal formation in the model.
PubMed: 36013429
DOI: 10.3390/life12081250 -
Saudi Journal of Biological Sciences Mar 2023Hexavalent chromium, toxic heavy metal, among the top-rated environmental contaminants, is declared a potent endocrine disruptor in humans and animals. The present study...
Hexavalent chromium, toxic heavy metal, among the top-rated environmental contaminants, is declared a potent endocrine disruptor in humans and animals. The present study was planned to find harmful effects on the reproductive system caused by Cr (VI) and the ameliorative effect of and -mediated AgNP on male mice (). In the present study, known infertility medicine, clomiphene citrate is also used as a positive control. The main objective of the present study was to assess the ameliorative potential of oral administration of a dose of 50 mg/kg BW clomiphene citrate (control), AgNP via chemical synthesis, seed extract, and -mediated AgNP against the Cr (VI) at the dose of 1.5 mg/kg BW from KCrO orally induced toxicity over eight weeks on the reproductive performance of male albino mice. mediated AgNPs were characterized by UV, SEM, FTIR, and XRD. The histological analysis, smear study, antioxidant capacity test, and hormone analysis were conducted by blood samples of albino mice. Cr exposed groups showed a significant decrease in sperm head breadth (5.29 ± 0.54 µ) and length (19.54 ± 1.18 µ), middle piece length, tail length, LH (1.65 ± 0.15 ng/mL), testosterone (2.63 ± 0.29 ng/mL), SOD (61.40 ± 2.48 mmol/mL), CAT (87.40 ± 6.01 mmol/mL), GSH (1.54 ± 0.09 µmol/mL), and no of spermatogonia (1.22 ± 0.25), and spermatocytes (2.33 ± 0.943). However, FSH level (160.00 ± 4.98 ng/mL), seminiferous tubule CSA (1094.69 ± 49.76 mm), size of spermatogonia (41.30 ± 1.24 µ), and spermatocytes (26.07 ± 1.34 µ) were significantly increased. Administration of and -mediated AgNPs reduced the toxicity.
PubMed: 36860759
DOI: 10.1016/j.sjbs.2023.103570 -
Urology Annals 2024To determine the utilization of barley and parsley for managing urolithiasis among the Saudi Arabian population.
OBJECTIVE
To determine the utilization of barley and parsley for managing urolithiasis among the Saudi Arabian population.
METHODS
This is a prospective cross-sectional survey-based study. The survey comprised questions about the use of barley, parsley, and other therapies for managing urolithiasis. A WhatsApp® message with the link to the study survey was sent out to family, friends, patients, and other acquaintances residing in the Kingdom of Saudi Arabia (KSA).
RESULTS
A total of 1014 respondents completed the survey, of which 44.8% indicated that they utilized barley, 38.3% stated that they used parsley, and 4.2% indicated that they utilized other non-medical remedies to treat or prevent kidney stones. In contrast, only 29.5% stated that they utilized potassium citrate and/or magnesium citrate, and only 14.4% indicated that they consumed greater amounts of water to treat or prevent kidney stones.
CONCLUSION
Our study findings indicate that among the Saudi Arabian population, non-conventional therapies such as barley and parsley are more commonly utilized for managing urolithiasis rather than established therapies such as increasing water intake and the use of potassium-citrate/ magnesium-citrate. There is a need to conduct large-scale clinical studies to evaluate the efficacy and safety of barley, parsley, and other non-conventional therapies for treating urolithiasis.
PubMed: 38818429
DOI: 10.4103/ua.ua_142_22 -
Animals : An Open Access Journal From... Dec 2022The clinical records of all guinea pigs diagnosed with a lower urinary tract disease in a single veterinary hospital, over a period of 14 years (2004−2018), were...
The clinical records of all guinea pigs diagnosed with a lower urinary tract disease in a single veterinary hospital, over a period of 14 years (2004−2018), were retrospectively searched in order to characterize this population and investigate the potential association between the epidemiological and clinical variables. A total of 117 clinical cases were identified, corresponding to 57 animals. Urolithiasis was the most common diagnosis (n = 52; 44.4%), followed by cystitis and/or a urinary tract infection (UTI). Several statistically significant associations (p < 0.05) were found between different variables, showing that female guinea pigs were more likely than the male ones to have a previous family history of urinary disease, to present dysuria and stranguria at admission, and to suffer recurrence. Moreover, males were more prone to urolithiasis and females to cystitis/UTI, and animals diagnosed with cystitis/UTI frequently had more clinical urinary signs and abdominal pain on palpation compared to those diagnosed with urolithiasis. Finally, the use of potassium citrate and the urethrotomy approach were associated with a better therapeutic response. Further studies are needed in larger populations of guinea pigs to confirm the present findings, especially as some of them were described for the first time.
PubMed: 36611721
DOI: 10.3390/ani13010112 -
Nutrients Jun 2024Hip fractures are a major health issue considerably impacting patients' quality of life and well-being. This is particularly evident in elderly subjects, in which the... (Review)
Review
Hip fractures are a major health issue considerably impacting patients' quality of life and well-being. This is particularly evident in elderly subjects, in which the decline in bone and muscle mass coexists and predisposes individuals to fall and fracture. Among interventions to be implemented in hip fractured patients, the assessment and management of nutritional status is pivotal, particularly in subjects older than 65. Nutrition plays a central role in both primary and secondary preventions of fracture. An adequate protein intake improves muscle mass and strength and the intestinal absorption of calcium. Other nutrients with recognized beneficial effects on bone health are calcium, vitamins D, K, and C, potassium, magnesium, folate, and carotenoids. With reference to calcium, results from longitudinal studies showed that the consumption of dairy foods has a protective role against fractures. Moreover, the most recent systematic reviews and meta-analyses and one umbrella review demonstrated that the combination of calcium and vitamin D supplementation significantly reduces hip fracture risk, with presumed higher efficacy in older and institutionalized subjects. Owing to these reasons, the adequate intake of calcium, vitamin D, protein, and other macro and micronutrients has been successfully implemented in the Fracture Liaison Services (FLSs) that represent the most reliable model of management for hip fracture patients. In this narrative review, papers (randomized controlled trials, prospective and intervention studies, and systematic reviews) retrieved by records from three different databases (PubMed, Embase, and Medline) have been analyzed, and the available information on the screening, assessment, and management of nutritional and vitamin D status and calcium intake in patients with hip fractures is presented along with specific prevention and treatment measures.
Topics: Humans; Hip Fractures; Vitamin D; Nutritional Status; Aged; Dietary Supplements; Calcium, Dietary; Female; Aged, 80 and over; Male; Musculoskeletal System; Calcium
PubMed: 38892706
DOI: 10.3390/nu16111773 -
Medical Journal of the Islamic Republic... 2022Urolithiasis is a common, sever, painful, and costly disease with a high probability of relapse. This study was performed to compare the effect of Polycitra-K...
Urolithiasis is a common, sever, painful, and costly disease with a high probability of relapse. This study was performed to compare the effect of Polycitra-K containing potassium citrate and Bicitra containing sodium citrate in the treatment of kidney stones in children who referred to Hazrat Masoumeh hospital in Qom. This double-blind randomized clinical trial study was carried out on 176 patients aged between 5 and 18 years old with kidney stones, hypocitraturia, and negative urine who referred to Hazrat Masoumeh hospital in Qom (Iran). Patients were divided into 2 groups of treatment (a dose of 1 mL/kg or 1-1.5 mg/ kg Polycitra-K) and control (Bicitra in the same dose). The results of kidney ureter bladder X ray (KUB ) was followed and the 2 groups were compared. The chi-square test or the Fisher exact test was used to analyze qualitative values in the treated groups. Regarding bladder stones, there was a significant difference between the 2 treatment groups ( = 0.025), in which16 patients (18.2%) in the Polycitrat-K group and 29 patients (33%) in the Bicitra group had bladder stones. With respect to stone passage, 58 patients (65.9%) in the Polycitra-K group and 36 patients (40.9%) in the Bicitra group were recorded. Oral Polycitrat-K is an effective preferential supplement against kidney stones in children due to urine alkalization, but the results of our study showed that both Polycitrat-K and Bicitra drugs have similar effects as therapeutic agents. Registration code in the Iranian Registry of Clinical Trials: IRCT20190619043945N1.
PubMed: 36128269
DOI: 10.47176/mjiri.36.70