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Data in Brief Jun 2023sp. IMCC1007 is a gram-negative, aerobic bacterium affiliated with class Betaproteobacteria, which was successfully isolated from maize rhizospheric soil sample in UTM...
sp. IMCC1007 is a gram-negative, aerobic bacterium affiliated with class Betaproteobacteria, which was successfully isolated from maize rhizospheric soil sample in UTM research plot, Pagoh, Malaysia by using enrichment method. Strain IMCC1007 utilized 50 mgL fusaric acid as its carbon source and degraded it completely within 14 h. Genome sequencing was performed using Illumina NovaSeq platform. The assembled genome was annotated using RAST (Rapid Annotation Subsystem Technology) server. The genome size was approximately 8,568,405 base pairs (bp) in 147 contigs with a G+C content of 66.04%. The genome includes 8,733 coding sequences and 68 RNAs. The genome sequence has been deposited at GenBank with the accession number of JAPVQY000000000. In the pairwise genome-to-genome comparisons, the strain IMCC1007 had an average nucleotide identity (ANI) of 91.9% and digital DNA-DNA hybridization (dDDH) value of 55.2% with DSM 16086 respectively. Interestingly, fusaric acid resistance gene (C) and ABCDFXT gene clusters (hydroxylation of pyridine compound) were found in the genome. Additionally, preliminary genome annotation analysis of strain IMCC1007 identified tryptophan halogenase (A) gene responsible for antifungal pyrrolnitrin biosynthesis. This dataset herein provides further insights into the fusaric acid degradation mechanism of the genus .
PubMed: 37383771
DOI: 10.1016/j.dib.2023.109204 -
Frontiers in Fungal Biology 2021is a major fungal pathogen causing life threatening infections in immunocompromised humans and certain animals. The HOG pathway is for two reasons interesting in this...
is a major fungal pathogen causing life threatening infections in immunocompromised humans and certain animals. The HOG pathway is for two reasons interesting in this context: firstly, it is a stress signaling pathway that contributes to the ability of this pathogen to adapt to various stress conditions and secondly, it is the target of antifungal agents, such as fludioxonil or pyrrolnitrin. In this study, we demonstrate that Ypd1 is an essential protein in . As the central component of the multistep phosphorelay it represents the functional link between the sensor histidine kinases and the downstream response regulators SskA and Skn7. A GFP-Ypd1 fusion was found to reside in both, the cytoplasm and the nucleus and this pattern was only slightly affected by fludioxonil. A strain in which the 1 gene is expressed from a tet-on promoter construct is unable to grow under non-inducing conditions and shows the characteristic features of wild type hyphae treated with fludioxonil. Expression of wild type Ypd1 prevents this lethal phenotype, but expression of an Ypd1 mutant protein lacking the conserved histidine at position 89 was unable to do so, which confirms that Ypd1 is a phosphotransfer protein. Generation of 1 variants of several mutant strains revealed that the lethal phenotype associated with low amounts of Ypd1 depends on SskA, but not on TcsC or Skn7. The ΔA 1, but not the ΔAΔ7 1 mutant, was sensitive to fludioxonil, which underlines the importance of Skn7 in this context. We finally succeeded to delete 1, but only if A and 7 were both inactivated, not in a ΔA single mutant. Hence, a deletion of 1 and an inactivation of Ypd1 by fludioxonil result in similar phenotypes and the two response regulators SskA and Skn7 are involved in both processes albeit with a different relative importance.
PubMed: 37744118
DOI: 10.3389/ffunb.2021.756990