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Materials (Basel, Switzerland) Sep 2019The surface of Q235 low carbon steel was modified by the metal inert-gas welding (MIG) method; a 304 stainless steel surfacing layer was fabricated to improve the...
The surface of Q235 low carbon steel was modified by the metal inert-gas welding (MIG) method; a 304 stainless steel surfacing layer was fabricated to improve the properties of Q235 low carbon steel. For practical industry application, keyhole tungsten inter gas (K-TIG) welding was used to weld the surface-modified plates. The microstructure, elemental distribution, micro-hardness, and corrosion resistance of the surface-modified plates and the welded joints were analyzed. The corrosion tests of welded joints and surface-modified plates were carried out with the electrochemical method and hydrochloric acid immersion method, respectively, and surface morphology after corrosion was studied. The results show that the surface-modified plates and their welded joints were defect-free. The microstructure of the surfacing layer consisted of austenite, martensite, and ferrite; and the microstructure of the weld consisted mainly of martensite. The hardness and corrosion resistance of the surfacing layer was superior to that that of low carbon steel. The micro-hardness of the weld is higher than that of the stainless steel surfacing layer and the base material. The corrosion resistance of the surfacing layer is the best, and the corrosion resistance of the welding seam is better than that of the base material.
PubMed: 31489923
DOI: 10.3390/ma12182883 -
Microorganisms Feb 2023, a nosocomial pathogen, is an emerging gut pathobiont causing antibiotic-associated diarrhea. infection involves gut colonization and disruption of the gut epithelial... (Review)
Review
, a nosocomial pathogen, is an emerging gut pathobiont causing antibiotic-associated diarrhea. infection involves gut colonization and disruption of the gut epithelial barrier, leading to the induction of inflammatory/immune responses. The expression of two major exotoxins, TcdA and TcdB is the major cause of pathogenicity. Attachment of bacterial abundant cell wall proteins or surface S-layer proteins (SLPs) such as SlpA with host epithelial cells is critical for virulence. In addition to being toxins, these surface components have been shown to be highly immunogenic. Recent studies indicate that SLPs play important roles in the adhesion of the bacteria to the intestinal epithelial cells, disruption of tight junctions, and modulation of the immune response of the host cells. These proteins might serve as new targets for vaccines and new therapeutic agents. This review summarizes our current understanding of the immunological role of SLPs in inducing host immunity and their use in the development of vaccines and novel therapeutics to combat infection.
PubMed: 36838345
DOI: 10.3390/microorganisms11020380 -
In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium.Scientific Reports Jul 2021Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with...
Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO, metastable CaZrO, and Ca(PO) were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli.
PubMed: 34302008
DOI: 10.1038/s41598-021-94502-z -
Nanomaterials (Basel, Switzerland) Nov 2023The binding of conidia to surfaces is a prerequisite for biofouling by fungal species. In this study, subtypes 1957 and 1988 were used which produced differently shaped...
The binding of conidia to surfaces is a prerequisite for biofouling by fungal species. In this study, subtypes 1957 and 1988 were used which produced differently shaped conidia (round or spikey respectively). Test surfaces were characterised for their surface topography, wettability, and hardness. Conidial assays included perpendicular and lateral force measurements, as well as attachment, adhesion and retention assays. Anionic surfaces were less rough ( 2.4 nm), less wettable (54°) and harder (0.72 GPa) than cationic surfaces ( 5.4 nm, 36° and 0.5 GPa, respectively). Perpendicular and lateral force assays demonstrated that both types of conidia adhered with more force to the anionic surfaces and were influenced by surface wettability. Following the binding assays, fewer 1957 and 1988 conidia bound to the anionic surface. However, surface wettability affected the density and dispersion of the conidia on the coatings, whilst clustering was affected by their spore shapes. This work demonstrated that anionic surfaces were more repulsive to 1998 spores than cationic surfaces were, but once attached, the conidia bound more firmly to the anionic surfaces. This work informs on the importance of understanding how conidia become tightly bound to surfaces, which can be used to prevent biofouling.
PubMed: 37999286
DOI: 10.3390/nano13222932 -
Developmental Cell Oct 2023Proper localization of receptors for synaptic organizing factors is crucial for synapse formation. Wnt proteins promote synapse assembly through Frizzled (Fz) receptors....
Proper localization of receptors for synaptic organizing factors is crucial for synapse formation. Wnt proteins promote synapse assembly through Frizzled (Fz) receptors. In hippocampal neurons, the surface and synaptic localization of Fz5 is regulated by neuronal activity, but the mechanisms involved remain poorly understood. Here, we report that all Fz receptors can be post-translationally modified by S-acylation and that Fz5 is S-acylated on three C-terminal cysteines by zDHHC5. S-acylation is essential for Fz5 localization to the cell surface, axons, and presynaptic sites. Notably, S-acylation-deficient Fz5 is internalized faster, affecting its association with signalosome components at the cell surface. S-acylation-deficient Fz5 also fails to activate canonical and divergent canonical Wnt pathways. Fz5 S-acylation levels are regulated by the pattern of neuronal activity. In vivo studies demonstrate that S-acylation-deficient Fz5 expression fails to induce presynaptic assembly. Our studies show that S-acylation of Frizzled receptors is a mechanism controlling their localization and function.
Topics: Animals; Rodentia; Frizzled Receptors; Wnt Signaling Pathway; Hippocampus; Acylation
PubMed: 37557176
DOI: 10.1016/j.devcel.2023.07.012 -
Materials (Basel, Switzerland) Dec 2022Surface topography in general is not easy to characterize due to a great number of different features that appear on it. It is still more challenging for machined...
Surface topography in general is not easy to characterize due to a great number of different features that appear on it. It is still more challenging for machined textured surfaces that are of high functional significance for tribological purposes. For practical reasons, there is a need to describe such surfaces using only a small number of parameters. Which of them represent surface details the best is still an open issue. To find out which parameters can be the most suitable in that case, three groups of machined textured surfaces were prepared. They were plateau-honed cylinder surfaces made of gray cast iron, steel, and bronze surfaces with isolated dimples and steel surfaces after abrasive blasting followed by lapping. All of them were measured by means of a white light interferometer. Different parameters and relationships were evaluated and based on them correlation and regression analyses were used. The basic description contained statistically independent parameters that can be used in production control, while the wider description in scientific research. In general, parameters of random surfaces were more intercorrelated than those of surfaces with isolated dimples. As was found for the basic description of random two-process surfaces, five parameters were enough while description of textured surfaces with isolated oil pockets needed six. In wider, scientific description, regardless the surface type seven parameters contained the necessary information about the surface. It was also proved that a pair of parameters, the emptiness coefficient Sp/Sz and Sq/Sa, can describe the shape of the ordinate distribution of machined textured surfaces better than, for example, skewness Ssk and kurtosis Sku, commonly used for that purpose.
PubMed: 36614502
DOI: 10.3390/ma16010163 -
Surfaces: a software to quantify and visualize interactions within and between proteins and ligands.Bioinformatics (Oxford, England) Oct 2023Computational methods for the quantification and visualization of the relative contribution of molecular interactions to the stability of biomolecular structures and...
SUMMARY
Computational methods for the quantification and visualization of the relative contribution of molecular interactions to the stability of biomolecular structures and complexes are fundamental to understand, modulate and engineer biological processes. Here, we present Surfaces, an easy to use, fast and customizable software for quantification and visualization of molecular interactions based on the calculation of surface areas in contact. Surfaces calculations shows equivalent or better correlations with experimental data as computationally expensive methods based on molecular dynamics.
AVAILABILITY AND IMPLEMENTATION
All scripts are available at https://github.com/NRGLab/Surfaces. Surface's documentation is available at https://surfaces-tutorial.readthedocs.io/en/latest/index.html.
Topics: Software; Proteins; Molecular Dynamics Simulation; Documentation; Ligands
PubMed: 37788107
DOI: 10.1093/bioinformatics/btad608 -
Molecules (Basel, Switzerland) Dec 2021Solid wettability is especially important for biomaterials and implants in the context of microbial adhesion to their surfaces. This adhesion can be inhibited by changes...
Effect of Spontaneous and Water-Based Passivation on Components and Parameters of Ti6Al4V (ELI Grade) Surface Tension and Its Wettability by an Aqueous Solution of Sucrose Ester Surfactants.
Solid wettability is especially important for biomaterials and implants in the context of microbial adhesion to their surfaces. This adhesion can be inhibited by changes in biomaterial surface roughness and/or its hydrophilic-hydrophobic balance. The surface hydrophilic-hydrophobic balance can be changed by the specifics of the surface treatment (proper conditions of surface preparation) or adsorption of different substances. From the practical point of view, in systems that include biomaterials and implants, the adsorption of compounds characterized by bacteriostatic or bactericidal properties is especially desirable. Substances that are able to change the surface properties of a given solid as a result of their adsorption and possess at least bacteriostatic properties include sucrose ester surfactants. Thus, in our studies the analysis of a specific surface treatment effect (proper passivation conditions) on a biomaterial alloy's (Ti6Al4V ELI, Grade 23) properties was performed based on measurements of the contact angles of water, formamide and diiodomethane. In addition, the changes in the studied solid surface's properties resulting from the sucrose monodecanoate (SMD) and sucrose monolaurate (SML) molecules' adsorption at the solid-water interface were also analyzed. For the analysis, the values of the contact angles of aqueous solutions of SMD and SML were measured at 293 K, and the surface tensions of the aqueous solutions of studied surfactants measured earlier were tested. From the above-mentioned tests, it was found that water environment significantly influences the components and parameters of Ti6Al4V ELI's surface tension. It also occurred that the addition of both SMD and SML to water (separately) caused a drop in the water contact angle on Ti6Al4V ELI's surface. However, the sucrose monolaurate surfactant is characterized by a slightly better tendency towards adsorption at the solid-water interface in the studied system compared to sucrose monodecanoate. Additionally, based on the components and parameters of Ti6Al4V ELI's surface tension calculated from the proper values of components and parameters of model liquids, it was possible to predict the wettability of Ti6Al4V ELI using the aqueous solutions of SMD and SML at various concentrations in the solution.
PubMed: 35011408
DOI: 10.3390/molecules27010179 -
Applied and Environmental Microbiology Mar 2023causes severe gastrointestinal disease resulting from the ingestion of oocysts, followed by oocyst excystation in the small intestine and the release of infective...
causes severe gastrointestinal disease resulting from the ingestion of oocysts, followed by oocyst excystation in the small intestine and the release of infective sporozoites. An understudied strategy for inactivation is purposeful oocyst excystation, as sporozoites do not survive long in the environment. This study showed that C. parvum oocyst excystation was induced by direct contact with various glycosaminoglycans (GAGs), including heparin (Hep), chondroitin sulfate A (CSA), and hyaluronan (HA), assembled on polydopamine (PD)-functionalized surfaces. PD surfaces elicited 97.9 ± 3.6% oocyst attachment, with some of the attached oocysts partially (7.3 ± 1.3%) or fully (4.0 ± 0.6%) excysted after 4 days. The PD-GAG surfaces (GAG concentration = 2 mg/mL) elicited similarly high attachment (>97%) and higher oocyst excystation efficiencies after 4 days. The PD-Hep surfaces elicited the highest number of attached excysted oocysts (11.8 ± 0.63% partially excysted; 11.9 ± 0.49% fully excysted), and the PD-HA surfaces elicited the lowest (8.8 ± 2.1% partially excysted; 7.8 ± 1.2% fully excysted). Surface characterization revealed that the addition of GAGs to the PD surface changed both the surface roughness as well as the surface wettability. Treatment of oocysts with an enzyme that degraded the surface glycocalyx markedly reduced excystation (to <2%) of the oocysts attached to the PD and PD-GAG surfaces. These findings suggest that GAGs provide an important local signal for the excystation of C. parvum oocysts and that certain surface-expressed oocyst receptors are necessary for efficient excystation. These oocyst-receptor relationships may be useful in the design of functionalized surfaces for the purposeful inactivation of oocysts in the environment or in water treatment systems. Polydopamine surfaces functionalized with glycosaminoglycans were shown to facilitate the attachment and excystation of Cryptosporidium parvum oocysts. Our findings suggest that a surface-expressed receptor on the oocyst wall plays a key role in excystation, with glycosaminoglycans serving as ligands that trigger the initiation of the process. Future technologies and treatment strategies designed to promote premature excystation of oocysts will minimize the ingestion of sporozoites that initiate infection. Therefore, the results from this study have important implications for the protection of public health from waterborne cryptosporidiosis and may serve as a foundation for engineered surfaces designed to remove oocysts from surface waters or inactivate oocysts in water treatment systems.
Topics: Animals; Cryptosporidium; Cryptosporidiosis; Glycosaminoglycans; Oocysts; Cryptosporidium parvum
PubMed: 36790186
DOI: 10.1128/aem.01737-22 -
Frontiers in Chemistry 2020The surface wettability of plants exhibits many unique advantages, which enhances the environmental adaptability of plants. In view of the rapid development of... (Review)
Review
The surface wettability of plants exhibits many unique advantages, which enhances the environmental adaptability of plants. In view of the rapid development of responsive materials, smart surfaces have been explored extensively to regulate surface wettability through external stimuli. Herein, we summarized recent advancements in bioinspired surfaces with switchable wettability. Typical bioinspired surfaces with switchable wettability and their emerging applications have been reviewed. In the end, we have discussed the remaining challenges and provided perspective on future development.
PubMed: 32903458
DOI: 10.3389/fchem.2020.00692