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Trends in Microbiology Sep 2019
Topics: Biofilms; Cholera; Cholera Toxin; Humans; Quorum Sensing; Vibrio cholerae; Virulence Factors
PubMed: 31029488
DOI: 10.1016/j.tim.2019.03.005 -
Essays in Biochemistry Jul 2021Vibrio natriegens is emerging as a promising host for biotechnology which is basically due to the remarkable intrinsic properties such as the exceptionally high growth... (Review)
Review
Vibrio natriegens is emerging as a promising host for biotechnology which is basically due to the remarkable intrinsic properties such as the exceptionally high growth and substrate consumption rates. The facultatively anaerobic marine bacterium possesses a versatile metabolism, is able to utilize a variety of substrates as carbon and energy sources and is easy to handle in the lab. These features initiated the rapid development of genetic tools and resulted in extensive engineering of production strains in the past years. Although recent examples illustrate the potential of V. natriegens for biotechnology, a comprehensive understanding of the metabolism and its regulation is still lacking but essential to exploit the full potential of this bacterium. In this review, we summarize the current knowledge on the physiological traits and the genomic organization, provide an overview of the available genetic engineering tools and recent advances in metabolic engineering of V. natriegens. Finally, we discuss the obstacles which have to be overcome in order to establish V. natriegens as industrial production host.
Topics: Biotechnology; Metabolic Engineering; Synthetic Biology; Vibrio
PubMed: 33835156
DOI: 10.1042/EBC20200135 -
Annual Review of Microbiology Sep 2022Cholera is a severe diarrheal disease caused by the bacterium and constitutes a significant public health threat in many areas of the world. infection elicits potent... (Review)
Review
Cholera is a severe diarrheal disease caused by the bacterium and constitutes a significant public health threat in many areas of the world. infection elicits potent and long-lasting immunity, and efforts to develop cholera vaccines have been ongoing for more than a century. Currently available inactivated two-dose oral cholera vaccines are increasingly deployed to both prevent and actively curb cholera outbreaks, and they are key components of the global effort to eradicate cholera. However, these killed whole-cell vaccines have several limitations, and a variety of new oral and nonoral cholera vaccine platforms have recently been developed. Here, we review emerging concepts in cholera vaccine design and implementation that have been driven by insights from human and animal studies. As a prototypical vaccine-preventable disease, cholera continues to be an excellent target for the development and application of cutting-edge technologies and platforms that may transform vaccinology.
Topics: Animals; Cholera; Cholera Vaccines; Humans; Vaccines, Inactivated; Vibrio cholerae
PubMed: 35759873
DOI: 10.1146/annurev-micro-041320-033201 -
Marine Drugs Dec 2021is a Gram-negative pathogenic bacterium that causes serious infections in humans and requires iron for growth. A clinical isolate, . M2799, secretes a catecholate... (Review)
Review
is a Gram-negative pathogenic bacterium that causes serious infections in humans and requires iron for growth. A clinical isolate, . M2799, secretes a catecholate siderophore, vulnibactin, that captures ferric ions from the environment. In the ferric-utilization system in . M2799, an isochorismate synthase (ICS) and an outer membrane receptor, VuuA, are required under low-iron conditions, but alternative proteins FatB and VuuB can function as a periplasmic-binding protein and a ferric-chelate reductase, respectively. The vulnibactin-export system is assembled from TolCV1 and several RND proteins, including VV1_1681. In heme acquisition, HupA and HvtA serve as specific outer membrane receptors and HupB is a sole periplasmic-binding protein, unlike FatB in the ferric-vulnibactin utilization system. We propose that ferric-siderophore periplasmic-binding proteins and ferric-chelate reductases are potential targets for drug discovery in infectious diseases.
Topics: Animals; Aquatic Organisms; Ions; Iron; Periplasmic Binding Proteins; Vibrio vulnificus
PubMed: 34940709
DOI: 10.3390/md19120710 -
Molecular Microbiology Sep 2020Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts.... (Review)
Review
Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways constitute a highly sophisticated cellular control system in Bacteria and Archaea. Reflecting the complex life cycle of V. cholerae, this organism has three different chemosensory pathways that together contain over 50 proteins expressed under different environmental conditions. Only one of them is known to control motility, while the function of the other two remains to be discovered. Here, we provide an overview of the chemosensory systems in V. cholerae and the advances toward understanding their structure and function.
Topics: Bacterial Proteins; Chemotaxis; Cholera; Humans; Signal Transduction; Vibrio cholerae; Virulence
PubMed: 32347610
DOI: 10.1111/mmi.14520 -
Microbiology (Reading, England) Nov 2020
Topics: Awards and Prizes; Biosensing Techniques; Cryptococcus neoformans; Directed Molecular Evolution; Drug Resistance, Bacterial; Homologous Recombination; Humans; Microbiology; Plant Diseases; Vibrio cholerae
PubMed: 33252324
DOI: 10.1099/mic.0.001005 -
Journal of Bacteriology Nov 2020is a large and diverse genus of bacteria, of which most are nonpathogenic species found in the aquatic environment. However, a subset of the genus includes several... (Review)
Review
is a large and diverse genus of bacteria, of which most are nonpathogenic species found in the aquatic environment. However, a subset of the genus includes several species that are highly pathogenic, either to humans or to aquatic animals. In recent years, , commonly known as the zebrafish, has emerged as a major animal model used for studying nearly every aspect of biology, including infectious diseases. Zebrafish are especially useful because the embryos are transparent, larvae are small and facilitate imaging studies, and numerous transgenic fish strains have been constructed. Zebrafish models for several pathogenic species have been described, and indeed a fish model is highly relevant for the study of aquatic bacterial pathogens. Here, we summarize the zebrafish models that have been used to study pathogenic species to date.
Topics: Animals; Disease Models, Animal; Humans; Vibrio; Vibrio Infections; Virulence; Zebrafish
PubMed: 32778562
DOI: 10.1128/JB.00165-20 -
Frontiers in Cellular and Infection... 2020, the causative agent of cholera, could proliferate in aquatic environment and infect humans through contaminated food and water. Enormous microorganisms residing in... (Review)
Review
, the causative agent of cholera, could proliferate in aquatic environment and infect humans through contaminated food and water. Enormous microorganisms residing in human gastrointestinal tract establish a special microecological system, which immediately responds to the invasion of , through "colonization resistance" mechanisms, such as antimicrobial peptide production, nutrients competition, and intestinal barrier maintenances. Meanwhile, could quickly sense those signals and modulate the expression of relevant genes to circumvent those stresses during infection, leading to successful colonization on the surface of small intestinal epithelial cells. In this review, we summarized the crosstalks profiles between gut microbiota and in the terms of Type VI Secretion System (T6SS), Quorum Sensing (QS), Reactive Oxygen Species (ROS)/pH stress, and Bioactive metabolites. These mechanisms can also be applied to molecular bacterial pathogenesis of other pathogens in host.
Topics: Bacterial Proteins; Cholera; Gastrointestinal Microbiome; Gene Expression Regulation, Bacterial; Humans; Intestines; Type VI Secretion Systems; Vibrio cholerae
PubMed: 33194819
DOI: 10.3389/fcimb.2020.582554 -
Nature Communications Dec 2022Small regulatory RNAs (sRNAs) acting in concert with the RNA chaperone Hfq are prevalent in many bacteria and typically act by base-pairing with multiple target...
Small regulatory RNAs (sRNAs) acting in concert with the RNA chaperone Hfq are prevalent in many bacteria and typically act by base-pairing with multiple target transcripts. In the human pathogen Vibrio cholerae, sRNAs play roles in various processes including antibiotic tolerance, competence, and quorum sensing (QS). Here, we use RIL-seq (RNA-interaction-by-ligation-and-sequencing) to identify Hfq-interacting sRNAs and their targets in V. cholerae. We find hundreds of sRNA-mRNA interactions, as well as RNA duplexes formed between two sRNA regulators. Further analysis of these duplexes identifies an RNA sponge, termed QrrX, that base-pairs with and inactivates the Qrr1-4 sRNAs, which are known to modulate the QS pathway. Transcription of qrrX is activated by QrrT, a previously uncharacterized LysR-type transcriptional regulator. Our results indicate that QrrX and QrrT are required for rapid conversion from individual to community behaviours in V. cholerae.
Topics: Humans; Vibrio cholerae; RNA
PubMed: 36482060
DOI: 10.1038/s41467-022-35261-x -
Nihon Saikingaku Zasshi. Japanese... 2021
Topics: Bacterial Proteins; Escherichia coli; Flagella; Molecular Motor Proteins; Vibrio
PubMed: 33627525
DOI: 10.3412/jsb.76.1