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The Practising Midwife 2012The aim of this bi-monthly column is to highlight Cochrane Systematic Reviews of relevance to pregnancy and childbirth and to stimulate discussion on the relevance and... (Review)
Review
The aim of this bi-monthly column is to highlight Cochrane Systematic Reviews of relevance to pregnancy and childbirth and to stimulate discussion on the relevance and implications of the review for practice. The Cochrane Collaboration is an international organisation that prepares and maintains high quality systematic reviews to help people make well-informed decisions about healthcare and health policy. A systematic review tries to search for, appraise and synthesise existing research to answer a specific research question. The Cochrane Database of Systematic Reviews (CDSR) is published monthly online. Residents in countries with a national license to The Cochrane Library, including the UK and Ireland, can access the Cochrane Library online free through www.thecochranelibrary.com.
Topics: Abortion, Incomplete; Abortion, Spontaneous; Adult; Bed Rest; Dilatation and Curettage; Evidence-Based Medicine; Female; Humans; Maternal Welfare; Pregnancy; Prenatal Care; Ultrasonography; Vacuum Curettage; Watchful Waiting; Young Adult
PubMed: 22908503
DOI: No ID Found -
Fertility and Sterility Apr 2013To study the genetic association between methylenetetrahydrofolate reductase (MTHFR) A1298 polymorphism and recurrent pregnancy loss (RPL). (Meta-Analysis)
Meta-Analysis
OBJECTIVE
To study the genetic association between methylenetetrahydrofolate reductase (MTHFR) A1298 polymorphism and recurrent pregnancy loss (RPL).
DESIGN
Prospective case-control study, systematic review, and meta-analysis using an electronic database up to July 27, 2012.
SETTING
Meta-analysis of four studies on RPL and three studies on spontaneously aborted embryos, including the present study.
PATIENT(S)
A total of 129 RPL patients and 202 healthy control women with successful pregnancy were analyzed including 40 spontaneously aborted embryos and 40 aborted embryos as control samples. For meta-analysis, 1,080 case and 709 control subjects were included of RPL and 375 case and 384 control samples of spontaneously aborted embryos.
INTERVENTION(S)
Blood was collected by peripheral venous punctures, and spontaneously aborted embryos were collected by curettage or manual vacuum aspiration. Meta-analysis was done on the basis of heterogeneity of the studies.
MAIN OUTCOME MEASURE(S)
Genotyping was done by polymerase chain reaction (PCR)-restriction-fragment-length polymorphism (RFLP). DNA sequencing was used to ascertain PCR-RFLP results. Age-adjusted odds ratios were calculated by logistic regression analysis. Meta-analysis on this polymorphism was conducted to support our findings.
RESULT(S)
We found that presence of rare allele "C" and heterozygous and rare homozygous genotypes significantly increased the risk of RPL. No significant change in the fetal MTHFR A1298C genotype frequency was observed, regardless of chromosomal integrity. Meta-analysis of A1298C polymorphism on both RPL and in spontaneously aborted embryos showed significantly increased risk in the carriers of AC and CC genotypes.
CONCLUSION(S)
The data of the present study clearly suggests that MTHFR A1298C polymorphism is a genetic risk factor for pregnancy loss.
Topics: Abortion, Habitual; Abortion, Spontaneous; Adult; Case-Control Studies; Female; Fetus; Genotype; Humans; Hyperhomocysteinemia; India; Methylenetetrahydrofolate Reductase (NADPH2); Polymorphism, Single Nucleotide; Pregnancy; Prospective Studies; Risk Factors
PubMed: 23357458
DOI: 10.1016/j.fertnstert.2012.12.027