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Forensic Toxicology Jul 2023The effects of extended Δ-tetrahydrocannabinol (Δ-THC) exposure on estrogen receptor-positive human breast cancer MCF-7 cells have been investigated; however, the...
PURPOSE
The effects of extended Δ-tetrahydrocannabinol (Δ-THC) exposure on estrogen receptor-positive human breast cancer MCF-7 cells have been investigated; however, the effects of Δ-THC exposure for a shorter duration remain unclear. In this study, we sought to study whether Δ-THC stimulates the migration of MCF-7 cells under both estrogenic and estrogen-deprived conditions over a short period (approximately 6 h).
METHODS
MCF-7 cells were treated with Δ-THC under estrogenic or estrogen-deprived conditions, and cell migration was subsequently analyzed.
RESULTS
Δ-THC-stimulated migration of MCF-7 cells 6 h after exposure was only observed in the estrogen-deprived condition. However, Δ-THC-mediated migration was counteracted under estrogenic conditions without affecting cell proliferation and estrogen receptor expression during this period.
CONCLUSIONS
Δ-THC can stimulate MCF-7 cell migration under estrogen-deprived conditions; however, there is an interfering interaction between Δ-THC and the estrogenic milieu that influences the migration of MCF-7 cells.
Topics: Humans; Female; Breast Neoplasms; Dronabinol; Receptors, Estrogen; MCF-7 Cells; Estrogens; Estrone; Cell Movement
PubMed: 36583834
DOI: 10.1007/s11419-022-00655-5 -
Nutrition and Cancer 2021Carotenoids found in fruits and vegetables are compounds with significant biological activities. Epidemiological studies report that these compounds have significant...
Carotenoids found in fruits and vegetables are compounds with significant biological activities. Epidemiological studies report that these compounds have significant anticancer effects, as well reducing the risk of cancer. In the present study, we aimed to determine the effects of capsanthin, an important carotenoid of paprika, on expressions of proteins playing roles in the mitochondrial apoptosis pathway, in addition to its possible cytotoxic and genotoxic effects in MCF-7 cells. Furthermore, possible oxidant/anti-oxidant roles of capsanthin on MCF-7 cells were investigated. The viability of MCF-7 cells was significantly decreased after 24 h of capsanthin application. After Comet analysis, it was determined that the capsanthin caused DNA damage on a dose-dependent manner. Furthermore, Western blot analysis showed that capsanthin application increased p53 and Bax protein expressions and caused a decrease in Bcl-2 protein level. Capsanthin treatment decreased catalase and glutathione levels but increased lipid peroxidation. These results show that the capsanthin causes oxidative stress and DNA damage, and increases mitochondrial apoptotic mechanism-mediated cell death after p53 and Bax protein activations.
Topics: Apoptosis; DNA Damage; Humans; MCF-7 Cells; Xanthophylls
PubMed: 32933334
DOI: 10.1080/01635581.2020.1819347 -
Journal of the College of Physicians... Mar 2019To investigate the expression of zinc finger transcription factors-Snail and E-cadherin in adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant... (Comparative Study)
Comparative Study
OBJECTIVE
To investigate the expression of zinc finger transcription factors-Snail and E-cadherin in adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.
STUDY DESIGN
An experimental study.
PLACE AND DURATION OF STUDY
The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital, and Jiangsu Institute of Cancer Research, China, from April 2017 to March 2018.
METHODOLOGY
Real-time quantitative PCR technology was used to detect the expression levels of Snail mRNA and E-cadherin mRNA in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells. Western blot was used to detect the expression levels of proteins of Snail and E-cadherin in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.
RESULTS
The expression of Snail mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly higher than that in normal breast cells (p<0.001) and non-resistant MCF-7 cells (p<0.001). The expression of E-cadherin mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly lower than that in normal breast cells (p<0.001) and non-resistant MCF-7 cells (p<0.001).
CONCLUSION
Adriamycin-resistant human breast cancer MCF-7/ADM cell strains had high expression of Snail and low expression of E-cadherin. This points out to a new research direction for the targeted therapy of drug-resistant breast cancer cells, and provides clinical guidance for breast cancer therapy and prognosis evaluation.
Topics: Blotting, Western; Breast Neoplasms; Cadherins; China; Doxorubicin; Drug Resistance, Neoplasm; Female; Gene Expression Regulation, Neoplastic; Hospitals, University; Humans; MCF-7 Cells; Molecular Targeted Therapy; RNA, Messenger; Real-Time Polymerase Chain Reaction; Reference Values; Snail Family Transcription Factors; Tumor Cells, Cultured
PubMed: 30823950
DOI: 10.29271/jcpsp.2019.03.240 -
Current Drug Delivery 2021This study aimed to explore an affordable technique for the fabrication of Chitosan Nanoshuttles (CSNS) at the ultrafine nanoscale less than 100 nm with improved...
AIM
This study aimed to explore an affordable technique for the fabrication of Chitosan Nanoshuttles (CSNS) at the ultrafine nanoscale less than 100 nm with improved physicochemical properties, and cytotoxicity on the MCF-7 cell line.
BACKGROUND
Despite several studies reported that the antitumor effect of CS and CSNS could achieve intracellular compartment target ability, no enough information is available about this issue and further studies are required to address this assumption.
OBJECTIVES
The objective of the current study was to investigate the potential processing variables for the production of ultrafine CSNS (less than; 100 nm) using Box-Behnken Design factorial design (BBD). This was achieved through a study of the effects of processing factors, such as CS concentration, CS/TPP ratio, and pH of the CS solution, on PS, PDI, and ZP. Moreover, the obtained CSNS was evaluated for physicochemical characteristics, morphology. In addition, hemocompatibility and cytotoxicity using Red Blood Cells (RBCs) and MCF-7 cell lines were investigated.
METHODS
Box-Behnken Design factorial design (BBD) was used in the analysis of different selected variables. The effects of CS concentration, sodium tripolyphosphate (TPP) ratio, and pH on particle size, Polydispersity Index (PDI), and Zeta Potential (ZP) were measured. Subsequently, the prepared CS nanoshuttles were exposed to stability studies, physicochemical characterization, hemocompatibility, and cytotoxicity using red blood cells and MCF-7 cell lines as surrogate models for study.
RESULT
The present results revealed that the optimized CSNS has ultrafine nanosize, (78.3 ± 0.22 nm), homogenous with PDI (0.131 ± 0.11), and ZP (31.9 ± 0.25 mV). Moreover, CSNS has a spherical shape, amorphous in structure, and physically stable. Moreover, CSNS has biological safety as indicated by a gentle effect on red blood cell hemolysis, besides, the obtained nanoshuttles decrease MCF-7 viability.
CONCLUSION
The present findings concluded that the developed ultrafine CSNS has unique properties with enhanced cytotoxicity, thus promising for use in intracellular organelles drug delivery.
Topics: Breast Neoplasms; Chitosan; Drug Carriers; Female; Humans; MCF-7 Cells; Nanoparticles; Particle Size
PubMed: 32682379
DOI: 10.2174/1567201817666200719005440 -
Toxicology Jun 2024Tributyltin chloride (TBTC) is a ubiquitous environmental pollutant with various adverse effects on human health. Exosomes are cell - derived signaling and substance...
Tributyltin chloride (TBTC) is a ubiquitous environmental pollutant with various adverse effects on human health. Exosomes are cell - derived signaling and substance transport vesicles. This investigation aimed to explore whether exosomes could impact the toxic effects caused by TBTC via their transport function. Cytotoxicity, DNA and chromosome damage caused by TBTC on MCF-7 cells were analyzed with CCK-8, flow cytometry, comet assay and micronucleus tests, respectively. Exosomal characterization and quantitative analysis were performed with ultracentrifugation, transmission electron microscope (TEM) and bicinchoninic acid (BCA) methods. TBTC content in exosomes was detected with Liquid Chromatography-Mass Spectrometry (LC-MS). The impacts of exosomal secretion on the toxic effects of TBTC were analyzed. Our data indicated that TBTC caused significant cytotoxicity, DNA and chromosome damage effects on MCF-7 cells, and a significantly increased exosomal secretion. Importantly, TBTC could be transported out of MCF-7 cells by exosomes. Further, when exosomal secretion was blocked with GW4869, the toxic effects of TBTC were significantly exacerbated. We concluded that TBTC promoted exosomal secretion, which in turn transported TBTC out of the source cells to alleviate its toxic effects. This investigation provided a novel insight into the role and mechanism of exosomal release under TBTC stress.
Topics: Humans; Exosomes; Trialkyltin Compounds; MCF-7 Cells; DNA Damage; Biological Transport; Environmental Pollutants; Cell Survival
PubMed: 38801937
DOI: 10.1016/j.tox.2024.153844 -
Molecules (Basel, Switzerland) Aug 2023We investigated the anticancer mechanism of a chloroform extract of marine sponge () (sample C) in human breast adenocarcinoma (MCF-7) cells. Viability analysis using...
We investigated the anticancer mechanism of a chloroform extract of marine sponge () (sample C) in human breast adenocarcinoma (MCF-7) cells. Viability analysis using MTT and neutral red uptake (NRU) assays showed that sample C exposure decreased the proliferation of cells. Flow cytometric data exhibited reactive oxygen species (ROS), nitric oxide (NO), dysfunction of mitochondrial potential, and apoptosis in sample C-treated MCF-7 cells. A qPCR array of sample C-treated MCF-7 cells showed crosstalk between different pathways of apoptosis, especially , , and genes. Immunofluorescence analysis affirmed the localization of p53, bax, bcl2, MAPKPK2, PARP-1, and caspase-3 proteins in exposed cells. Bioassay-guided fractionation of sample C revealed Neviotin A as the most active compound triggering maximum cell death in MCF-7, indicating its pharmacological potency for the development of a drug for the treatment of human breast cancer.
Topics: Humans; Transcriptome; MCF-7 Cells; Gene Expression Profiling; Cell Death; Apoptosis
PubMed: 37687120
DOI: 10.3390/molecules28176289 -
Biomedicine & Pharmacotherapy =... Apr 2016APOBEC3B belongs to a protein family of cytidine deaminases that can insert mutations in DNA and RNA as a result of their ability to deaminate cytidine to uridine. It...
APOBEC3B belongs to a protein family of cytidine deaminases that can insert mutations in DNA and RNA as a result of their ability to deaminate cytidine to uridine. It has been shown that APOBEC3B-catalysed deamination provides a chronic source of DNA damage in breast cancers. We investigated APOBEC3B expression in four drug resistant breast cancer cell lines (Doxorubicin, Etoposide, Paclitaxel and Docetaxel resistant MCF-7 cell lines) using a novel RNA in situ hybridization technology (RNAscope) and compared expression levels with drug sensitive MCF-7 cell line. After RNAscope staining, slides were scanned and saved as digital images using Aperio scanner and software. Quantitative scoring utilizing the number of punctate dots present within each cell boundary was performed for the parameters including positive cell percentage and signal intensity per positive cell. In Doxorubicin and Etoposide resistant MCF-7 cell lines, APOBEC3B expression was approximately five-fold increased (23% and 24% respectively) with higher signal intensity (1.92 and 1.44 signal/cell, respectively) compared to drug sensitive MCF-7 cell line (5%, 1.00 signal/cell) with statistical significance. The increase of APOBEC3B expression in Docataxel resitant and Paclitaxel resistant MCF-7 cell lines was not very high. In conclusion, APOBEC3B expression was increased in some population of tumor cells of drug resistant cell lines. At least for some drugs, APOBEC3B expression may be related to drug resistance, subjecting to some tumor cells to frequent mutation.
Topics: Blotting, Western; Breast Neoplasms; Cytidine Deaminase; Drug Resistance, Neoplasm; Female; Humans; Immunohistochemistry; MCF-7 Cells; Minor Histocompatibility Antigens
PubMed: 27044816
DOI: 10.1016/j.biopha.2016.02.004 -
Environmental Science and Pollution... Jan 2020The present study deals with the synthesis of silver nanoparticles (AgNPs) from Rhynchosia rufescens and to evaluate its cytotoxic effect mediated through induced...
The present study deals with the synthesis of silver nanoparticles (AgNPs) from Rhynchosia rufescens and to evaluate its cytotoxic effect mediated through induced apoptosis. The reduction and capping of phytoconstituents was confirmed using FTIR demonstrating O-H and C-H stretching at different peaks. The size and the shape of the particle were determined using scanning electron microscopy (SEM) illustrating 1 μm to 100 nm in size and the composition of compounds in the AgNPs were revealed using XRD and EDX. The results of the antioxidant assays revealed that the synthesized AgNPs had significant radical scavenging potential in dose-dependent inhibition with 22-64% for DPPH and 25-41% for ferric reducing antioxidant power assay at the concentrations of 20-100 μg/ml. Further, the synthesized AgNPs demonstrated potent cytotoxic activity against human breast cancer (MCF-7) cell line with an IC value of 26 ± 1.0 μg/ml by the MTT assay. Cytotoxicity was confirmed using AO/EtBr and DAPI staining method where nuclear condensation and fragmentation of cancer cells was observed after treatment with nanoparticle. The results were further confirmed by flow cytometry analysis which revealed the occurrence of apoptosis during the S phase in cell cycle exposing the potential of the AgNPs against MCF-7 cancer cell. From the results, we conclude that the synthesized AgNPs from Rhynchosia rufescens exhibited multifunctional properties. Graphical abstract.
Topics: Apoptosis; Fabaceae; Free Radical Scavengers; Humans; MCF-7 Cells; Metal Nanoparticles; Reactive Oxygen Species; Silver
PubMed: 31773523
DOI: 10.1007/s11356-019-06479-y -
Bioorganic Chemistry Nov 2022Four undescribed seco-polyprenylated acylphloroglucinols (seco-PAPs), elodeoidesones A-D (1-4), were characterized from Hypericum elodeoides. Compound 1 represents the...
Four undescribed seco-polyprenylated acylphloroglucinols (seco-PAPs), elodeoidesones A-D (1-4), were characterized from Hypericum elodeoides. Compound 1 represents the 1,6-seco-PAPs with fascinating 5/5 fused ring, while 2-4 possess a 1,2-seco-PAPs skeleton with a five-membered lactone core. Their structures including absolute configurations were established by spectroscopic analyses and quantum chemical computations. A possible biosynthetic pathway of 1-4 from normal PAPs was proposed. All the isolates were investigated for their cytotoxicity against tumor cells. Notably, 1 inhibited the proliferation of MCF-7 cells with the IC value of 7.34 μM. Mechanism investigation indicated that 1 induced MCF-7 cells apoptosis by blocking cell cycle at S phase via inducing oxidative DNA damage.
Topics: Apoptosis; Cell Cycle Checkpoints; Humans; Hypericum; MCF-7 Cells; Molecular Structure; Oxidative Stress; Phloroglucinol
PubMed: 36007479
DOI: 10.1016/j.bioorg.2022.106088 -
Naunyn-Schmiedeberg's Archives of... May 2023Breast cancer is the most prevalent diagnosed cancer among women and the main cause of morbidity and mortality. As for breast cancer, MCF-7 cells are an important...
Breast cancer is the most prevalent diagnosed cancer among women and the main cause of morbidity and mortality. As for breast cancer, MCF-7 cells are an important candidate since they are widely utilized in research for estrogen receptor (ER)-positive breast cancer cell assays, and various sub-clones have been identified to reflect different classes of ER-positive tumors with varied levels of nuclear receptor expression. Rhodamines and its derivatives have shown a great interest over the past two decades due to their excellent structural and spectroscopic properties. Rhodamine derivatives have been widely investigated for their mitochondrial targeting and chemotherapeutic properties. Rhodamine derivatives, in particular, have been widely investigated for their therapeutic properties. In this regard, several studies have shown that rhodamine dye derivatives have promising in vitro and in vivo therapeutic efficacy. The present study deals with potential anticancer activity of few synthesized rhodamine derivatives against MCF-7 cell lines.
Topics: Female; Humans; Antineoplastic Agents; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; MCF-7 Cells; Rhodamines
PubMed: 36595094
DOI: 10.1007/s00210-022-02376-3