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Acta Biochimica Et Biophysica Sinica Mar 2019
Topics: Autophagy; Glycolysis; Humans; Intracellular Signaling Peptides and Proteins; MCF-7 Cells; Protein-Arginine N-Methyltransferases
PubMed: 30883646
DOI: 10.1093/abbs/gmz006 -
Molecular Biology Reports Jul 2022Edible-medicinal fungi are mainly used in Asian countries to prevent various diseases. These mushrooms are also used to treat lung diseases and cancer. Ganoderma lucidum...
INTRODUCTION
Edible-medicinal fungi are mainly used in Asian countries to prevent various diseases. These mushrooms are also used to treat lung diseases and cancer. Ganoderma lucidum and Lentinula edodes are the most important edible-medicinal fungi. The polysaccharides of these fungi are one of the bioactive compounds with anti-cancer properties.
OBJECTIVE
Evaluation of anti-cancer effects of Shiitake and Reishi polysaccharides.
METHODS
In this study, fungal polysaccharides were extracted using the hot water method and were purified by Diethylaminoethyl Sephadex A-25 (DEAE-Sephadex A-25) chromatography column and their concentration was measured by phenolic sulfuric acid method. The biological effects of the extracted polysaccharides from Ganoderma lucidum and Lentinula edodes on the MCF-7 cell line were investigated using an MTT assay and then its effects on the expression of the P53 cancer regulatory gene and HER-3 gene were investigated.
RESULTS
Based on the results, the concentration of Ganoderma lucidum and Lentinula edodes extracted polysaccharides were 0.024 and 0.103 mg/ml, respectively. Polysaccharides of these two fungi increased the expression of the P53 gene and decreased the expression of the HER-3 gene in a dose and time-dependent manner.
DISCUSSION
Natural biocompatible polysaccharides with anti-cancer properties that are native, are available, and inexpensive, so they can be used as dietary supplements to prevent and help treat cancer.
Topics: Biopolymers; Gene Expression; Humans; MCF-7 Cells; Polysaccharides; Reishi
PubMed: 35536497
DOI: 10.1007/s11033-022-07496-w -
Bioorganic & Medicinal Chemistry Letters Aug 2019Breast cancer is the most common female cancer. However, the known effective specific biomarkers for breast cancer are still scarce. Abnormal membrane proteins serve as...
Breast cancer is the most common female cancer. However, the known effective specific biomarkers for breast cancer are still scarce. Abnormal membrane proteins serve as ideal biomarkers for disease diagnoses, therapeutics and prognosis. Thus aptamers (single-stranded oligonucleotide molecules) with molecular recognition properties can be used as efficient tools to sort cells based on differences in cell surface architecture between normal and tumor cells. In this study, we aimed to screen specific aptamer against MCF-7 human breast cancer cells. Cell-SELEX process was performed to isolate aptamers from a combinatorial single-stranded nucleic acid library that selectively targeting surface proteins of MCF-7 cells in contrast with MCF-10A human mammary epithelial cells. The process was repeated until the pool was enriched for sequences that specifically recognizing MCF-7 cells in monitoring by flow cytometry. Subsequently, the enriched pool was cloned into bacteria, and positive clones were sequenced to obtain individual sequences. Representative sequences were chemically synthesized and evaluated their binding affinities to MCF-7 cells. As a result, an aptamer S1 was finally identified to have high binding affinity with equilibrium dissociation constant (K) value of 29.9 ± 6.0 nM. FAM-labeled aptamer S1 induced fluorescence shift in MCF-7 cells but not in MCF-10A human mammary epithelial cells, or MDA-MB-453 and MDA-MB-231 human breast cancer cells. Furthermore, result of cell imaging observed from laser confocal fluorescence microscope showed that MCF-7 cells exhibited stronger fluorescence signal resulted from Cy5-labeled aptamer S1 than MCF-10A cells. The above findings suggested that S1 may be a specificity and selectivity aptamer for MCF-7 cells and useful for the breast cancer detection and diagnosis.
Topics: Antineoplastic Agents; Aptamers, Nucleotide; Breast Neoplasms; Cell Line; Cell Proliferation; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Humans; MCF-7 Cells; Molecular Structure; Optical Imaging; SELEX Aptamer Technique; Structure-Activity Relationship
PubMed: 31196711
DOI: 10.1016/j.bmcl.2019.06.002 -
Free Radical Research Apr 2014In this study, we studied the mechanism of the cytotoxicity of malabaricone C (mal C) against human breast cancer MCF-7 cell line. Mal C dose-dependently increased the...
In this study, we studied the mechanism of the cytotoxicity of malabaricone C (mal C) against human breast cancer MCF-7 cell line. Mal C dose-dependently increased the sub G1 cell population, associated with cytoplasmic oligonucleosome formation and chromatin condensation. The mal C-induced apoptosis led to mitochondrial damage as revealed by fluorescence microscopy and flow cytometry of the JC-1-stained cells as well as from the release of mitochondrion-specific nuclease proteins AIF and endo G. Mal C also released intracellular Ca(2+) from the MCF-7 cells, but the Ca(2+)-modulators BAPTA-AM and Ru360 only partially abrogated the apoptosis. The calpain activation by mal C did not have any effect on its cytotoxicity. On the other hand, after mal C treatment significant lysosomal membrane permeabilization (LMP), along with release of cathepsin B, as well as Bid-cleavage and its translocation to mitochondria were observed much earlier than the mitochondrial damage. This suggested that cytotoxicity of mal C against human MCF-7 human breast cancer cell line may proceed through LMP as the initial event that triggered a caspase-independent, but cathepsin B and t-Bid-dependent intrinsic mitochondrial apoptotic pathway. A significant accumulation of cells in the S or G2-M phases along with upregulation of the cyclins E and A due to mal C exposure promises it to be a potential anti-cancer agent.
Topics: Apoptosis; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Female; Humans; MCF-7 Cells; Resorcinols; Signal Transduction
PubMed: 24456233
DOI: 10.3109/10715762.2014.886328 -
Journal of Pharmaceutical and... May 2022This study investigated the inhibitory effect of epirubicin combined with berberine on MCF-7 cell proliferation and explored intracellular pharmacokinetics. A CCK-8...
This study investigated the inhibitory effect of epirubicin combined with berberine on MCF-7 cell proliferation and explored intracellular pharmacokinetics. A CCK-8 assay was used to detect the inhibitory effect of epirubicin alone and in combination with berberine on MCF-7 cell proliferation in vitro. A rapid and sensitive LC-MS/MS method was developed and validated to detect epirubicin in MCF-7 cells. After incubation with epirubicin and berberine at different time points, MCF-7 cells were lysed and precipitated by acetonitrile to extract the analyte. Chromatographic separation was performed on a C column. Daunorubicin was selected as the internal standard. The analytical method was applied to determine the epirubicin concentration in MCF-7 cells. Berberine was found to enhance the inhibitory effect of epirubicin (1 μg/mL and 2 μg/mL) on the proliferation of MCF-7 cells (P < 0.05). LC-MS/MS analysis revealed that berberine significantly increased epirubicin concentration in MCF-7 cells at 8, 12, and 24 h (P < 0.05). The results suggest that berberine may enhance the inhibitory effect of epirubicin on cell proliferation by increasing the concentration of epirubicin in MCF-7 cells. The assay provides a basis for research on the intracellular pharmacokinetics of epirubicin and develops analytical methods for other cell-targeted drugs.
Topics: Berberine; Chromatography, Liquid; Epirubicin; Humans; MCF-7 Cells; Tandem Mass Spectrometry
PubMed: 35279450
DOI: 10.1016/j.jpba.2022.114692 -
Journal of Integrative Medicine Jan 2016To investigate the effects of ophiopogonin D on human breast cancer MCF-7 cells.
OBJECTIVE
To investigate the effects of ophiopogonin D on human breast cancer MCF-7 cells.
METHODS
Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation experiments. Cell cycle was measured with cell cycle flow cytometry and a living cell assay. Apoptosis and terminal deoxynucleoitidyl transferase-mediated dUTP nick end labeling assays were performed to detect the apoptosis of MCF-7 cells induced by ophiopogonin D. Finally, Western blotting was used to explore the mechanism.
RESULTS
Exposure of cells to ophiopogonin D resulted in marked decreases in viable cells and colony formation with a dose-dependent manner. Treatment of these cells with ophiopogonin D also resulted in cell cycle arrest at the G(2)/M phase, and increased apoptosis. Mechanistically, ophiopogonin D-induced G(2)/M cell cycle arrest was associated with down-regulation of cyclin B1. Furthermore, activation of caspase-8 and caspase-9 was involved in ophiopogonin D-induced apoptosis.
CONCLUSION
The data suggested that ophiopogonin D inhibits MCF-7 cell growth via the induction of cell cycle arrest at the G(2)/M phase.
Topics: Apoptosis; Cell Proliferation; G2 Phase Cell Cycle Checkpoints; Humans; M Phase Cell Cycle Checkpoints; MCF-7 Cells; Saponins; Spirostans
PubMed: 26778229
DOI: 10.1016/S2095-4964(16)60238-8 -
BMC Complementary Medicine and Therapies Jan 2020Nigella sativa (NS), a member of family Ranunculaceae is commonly known as black seed or kalonji. It has been well studied for its therapeutic role in various diseases,...
BACKGROUND
Nigella sativa (NS), a member of family Ranunculaceae is commonly known as black seed or kalonji. It has been well studied for its therapeutic role in various diseases, particularly cancer. Literature is full of bioactive compounds from NS seed. However, fewer studies have been reported on the pharmacological activity of proteins. The current study was designed to evaluate the anticancer property of NS seed proteins on the MCF-7 cell line.
METHODS
NS seed extract was prepared in phosphate-buffered saline (PBS), and proteins were precipitated using 80% ammonium sulfate. The crude seed proteins were partially purified using gel filtration chromatography, and peaks were resolved by SDS-PAGE. MTT assay was used to screen the crude proteins and peaks for their cytotoxic effects on MCF-7 cell line. Active Peaks (P1 and P4) were further studied for their role in modulating the expression of genes associated with apoptosis by real-time reverse transcription PCR. For protein identification, proteins were digested, separated, and analyzed with LC-MS/MS. Data analysis was performed using online Mascot, ExPASy ProtParam, and UniProt Knowledgebase (UniProtKB) gene ontology (GO) bioinformatics tools.
RESULTS
Gel filtration chromatography separated seed proteins into seven peaks, and SDS-PAGE profile revealed the presence of multiple protein bands. Among all test samples, P1 and P4 depicted potent dose-dependent inhibitory effect on MCF-7 cells exhibiting IC values of 14.25 ± 0.84 and 8.05 ± 0.22 μg/ml, respectively. Gene expression analysis demonstrated apoptosis as a possible cell killing mechanism. A total of 11 and 24 proteins were identified in P1 and P4, respectively. The majority of the proteins identified are located in the cytosol, associate with biological metabolic processes, and their molecular functions are binding and catalysis. Hydropathicity values were mostly in the hydrophilic range.
CONCLUSION
Our findings suggest NS seed proteins as a potential therapeutic agent for cancer. To our knowledge, it is the first study to report the anticancer property of NS seed proteins.
Topics: Antineoplastic Agents, Phytogenic; Apoptosis; Cell Proliferation; Chromatography, Gel; Humans; MCF-7 Cells; Mass Spectrometry; Nigella sativa; Pakistan; Plant Extracts; Plant Proteins; Seeds
PubMed: 32020890
DOI: 10.1186/s12906-019-2804-1 -
Nutrition and Cancer 2021In this study, we evaluated the cytotoxicity and apoptotic activity of (ZO), (TC) alone, and in combination (ZO:TC-1:4). The presence of major bioactive compounds in...
In this study, we evaluated the cytotoxicity and apoptotic activity of (ZO), (TC) alone, and in combination (ZO:TC-1:4). The presence of major bioactive compounds in ZO (6-gingerol and 6-shogaol) and TC (gallic acid, ellagic acid, and chebulinic acid) were evaluated by high performance liquid chromatography. The IC50 values of ZO, TC, and ZOTC (1:4) was estimated to be 88.5, 108.5, and 53.5 μg/mL, respectively. The cell death and cytomorphology changes upon treatment were observed. At these concentrations, ZO, TC, and ZOTC showed reduced mitochondrial membrane potential, increased reactive oxygen species, and apoptotic activities. It was also reported to downregulate mTOR and hTERT gene expression levels which are the primary genes for cell proliferation and growth. This first report on ZOTC combination has the potential to develop as a therapeutic agent for breast cancer.
Topics: Apoptosis; Gene Expression; Zingiber officinale; Humans; MCF-7 Cells; Plant Extracts; TOR Serine-Threonine Kinases; Terminalia
PubMed: 32664754
DOI: 10.1080/01635581.2020.1792518 -
Journal of Acupuncture and Meridian... Jun 2016Cancer is the cause of more than 6 million deaths worldwide every year. For centuries, medicinal plants have been used in the treatment of cancer. Chemotherapy,...
Cancer is the cause of more than 6 million deaths worldwide every year. For centuries, medicinal plants have been used in the treatment of cancer. Chemotherapy, radiotherapy, surgery and acupuncture point stimulation are also used to treat cancer. The present study was intended to reveal the cytotoxic and anticancer potential of selected Cyathea species and to highlight their importance in the pharmaceutical industry for the development of cost-effective drugs. Cytotoxic studies using brine shrimp lethality bioassays and MCF 7 cell line cultures were carried out. Compared to petroleum ether, chloroform and acetone extracts, the ethanol extracts of selected Cyathea species were found to be more effective against brine shrimps. The ethanol extracts were further subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assays. A decrease in cell viability and an increase in growth inhibition were observed for the MCF 7 cell line. The maximum percentage of cell inhibition was observed in Cyathea crinit, followed by Cyathea nilgirensis and Cyathea gigantea. The results of the present study suggest that Cyathea species are an effective source of cytotoxic compounds.
Topics: Cell Proliferation; Cell Survival; Ferns; Humans; MCF-7 Cells; Plant Extracts; Plant Leaves; Plants, Medicinal
PubMed: 27342889
DOI: 10.1016/j.jams.2016.04.004 -
Asian Pacific Journal of Cancer... 2012Discovery of anticancer drugs that kill or disable tumor cells in the presence of normal cells without undue toxicity is a potential challenge for therapeutic care....
Discovery of anticancer drugs that kill or disable tumor cells in the presence of normal cells without undue toxicity is a potential challenge for therapeutic care. Several papers in the literature have emphasized the potential implications of marine products such as seaweeds which exhibit antitumor activity. Study attempts to screen the antitumor effect of Sargassum sp, against chosen cell lines such as MCF-7 (Breast cancer) and Hep-2 (Liver Cancer). Ethanol extract of Sargassum sp. was concentrated using a Soxhlet apparatus and dissolved in DMSO. In vitro cytotoxic activity of Sargassum sp at various concentrations (100 μg/ml-300 μg/ml) screened for antitumor effect against the chosen cell lines using MTT assay (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole). The study documented that the percentage of cell viability has been reduced with increased concentration, as evidenced by cell death. Sargassum sp extract shows potential cytotoxic activity (P≤0.05) with IC50 of 200 μ g/ml and 250 μg/ml against Hep-2 and MCF-7 cell lines respectively. The ethanol fraction of Sargassum sp induced cell shrinkage, cell membrane blebbing and formation of apoptotic bodies with evidence of bioactive components as profound influencing factors for anti-tumor effects. Further research need to be explored for the successful application of Sargassum sp as a potent therapeutic tool against cancer.
Topics: Cell Line, Tumor; Cell Survival; Humans; MCF-7 Cells; Plant Extracts; Sargassum; Seaweed; Vegetables
PubMed: 23464406
DOI: 10.7314/apjcp.2012.13.12.6073