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Nihon Geka Gakkai Zasshi Sep 1991The prognostic significance of nuclear DNA ploidy patterns and the S-phase fraction (labeling index; IL) were evaluated in 365 gastrointestinal tumors, and in 62 of them...
The prognostic significance of nuclear DNA ploidy patterns and the S-phase fraction (labeling index; IL) were evaluated in 365 gastrointestinal tumors, and in 62 of them a combined analysis of DNA ploidy and the SPF was performed. For accurate evaluation, we used fresh frozen specimens, and we classified the ploidy pattern into 6 types; 1. diploid, 2. DS (diploid + high LI), 3. Notch, 4. Shoulder, 5. Tetraploid and 6. Aneuploid. Type 1 or type 2 tumors were classified as diploid, and the others were classified as aneuploid. In 103 cases of gastric cancer and 101 colon cancers due to the short observation period, but in 46 patients with operable primary liver cancer, a significant difference was observed. A high average of LI was detected in colon cancer (approximately 13%), but no relationship between LI and the ploidy pattern was found. This indicates that the LI may become an independent prognostic factor, and that the combined assay of DNA ploidy and the LI may offer a more precise evaluation of the malignant potential of gastrointestinal tumors.
Topics: DNA, Neoplasm; Gastrointestinal Neoplasms; Humans; Mitotic Index; Ploidies; Prognosis; S Phase
PubMed: 1944160
DOI: No ID Found -
Cell and Tissue Kinetics Nov 1984Circadian rhythms in epidermal basal cell-cycle progression in hairless mouse skin have been repeatedly demonstrated. A dose of 10 mg/animal hydroxyurea (HU), given to...
Circadian rhythms in epidermal basal cell-cycle progression in hairless mouse skin have been repeatedly demonstrated. A dose of 10 mg/animal hydroxyurea (HU), given to inhibit DNA synthesis was injected intraperitoneally to two groups of hairless mice. One group was injected at 10.00 hours MET, when the cell-cycle progression and cell division rate are relatively high, and another group was injected at 20.00 hours, when the same variables are at minimum values. Various cell kinetic methods--[3H]TdR autoradiography, DNA flow cytometry and the stathmokinetic method (Colcemid)--were used to study HU-induced alterations in cell kinetics. Hydroxyurea (HU) immediately reduced the labelling index (LI) to less than 10% of controls when injected at both times of the day, and higher then normal values were observed 8 hr later. A subsequent decrease towards normal values was steeper in the 20.00 hours injected group. The proportion of cells with S-phase DNA content was transiently reduced in both series, but the reduction was less pronounced and control values were reached earlier in the series injected at 10.00 hours. The observed alterations in LI and fraction of cells in S phase were followed by comparable alterations in the fraction of cells in G2 and in the mitotic rate. Hence the changes in G2 and mitotic rate are easily explained as consequences of the previous perturbations in the S phase. The time-dependent differences in the cell kinetic perturbations caused by HU in the S phase may be explained by a circadian-phase-dependent action of HU on the influx and efflux of cells to and from the S phase, respectively. At 10.00 hours the efflux of cells from S is most heavily inhibited; at 20.00 hours the influx is predominantly blocked. Hence, when physiological flux is high HU mainly blocks the efflux from S, but when flux normally is low, HU mainly blocks the entrance to S. Within 20 hours after the HU injection, the cell kinetic variables had approached the unperturbed circadian pattern.
Topics: Animals; Cell Cycle; DNA; DNA Replication; Flow Cytometry; Hydroxyurea; Interphase; Kinetics; Mice; Mice, Hairless; Mitotic Index; Skin; Tritium
PubMed: 6488273
DOI: 10.1111/j.1365-2184.1984.tb00614.x -
Human Pathology Jun 1992
Topics: Cytological Techniques; Humans; Mitotic Index; Neoplasms
PubMed: 1592380
DOI: 10.1016/0046-8177(92)90312-q -
The Journal of Investigative Dermatology Jan 1983
Topics: Cell Division; Circadian Rhythm; Humans; Kinetics; Mitotic Index; Psoriasis; Skin Physiological Phenomena
PubMed: 6848610
DOI: 10.1111/1523-1747.ep12531338 -
Advances in Experimental Medicine and... 1976
Topics: Animals; Antibody-Producing Cells; Cell Count; Chimera; Hemolytic Plaque Technique; Immunoglobulin G; Immunoglobulin M; Lymph Nodes; Mice; Mice, Inbred CBA; Mitotic Index; Mosaicism; Organ Size; Oxazolone; Spleen; T-Lymphocytes
PubMed: 773114
DOI: No ID Found -
Advances in Space Research : the... 1984In track segment experiments cell survival and chromosome aberrations of mammalian cells have been measured for various heavy ion beams between helium and uranium in the...
In track segment experiments cell survival and chromosome aberrations of mammalian cells have been measured for various heavy ion beams between helium and uranium in the energy range between 0.5 and 960 MeV/u, corresponding to a velocity range of 0.03 to 0.87 C, and an LET spectrum from 10 to 15 000 keV/micrometers. At low LET, the cross section (sigma) for cell killing increases with increasing LET and shows a common curve for all ions regardless of the atomic number. This indicates that in this region the track structure of the different ions is of only a minor influence, and it is rather the total energy transfer, which is important for cell killing. At higher LET values, deviations from a common sigma-LET curve can be observed which indicate a saturation effect. The saturation of the lighter ions occurs at lower LET values than for the heavier ions. These findings are also confirmed by the chromosome data, where the efficiency for the induction of chromosomal aberrations for high LET particles depends on the track structure and is nearly independent of LET. In the heavier beams (Z > or = 10) individual particles cause multiple chromosome breaks in mitotic cells.
Topics: Animals; Cell Cycle; Cell Line; Cell Physiological Phenomena; Cell Survival; Cells, Cultured; Chromosome Aberrations; Cricetinae; Electrons; Heavy Ions; Linear Energy Transfer; Mitosis; Mitotic Index; Particle Accelerators; Relative Biological Effectiveness
PubMed: 11539630
DOI: 10.1016/0273-1177(84)90244-8 -
IEEE/ACM Transactions on Computational... 2021This interdisciplinary work focuses on the interest of a new auto-encoder for supervised classification of live cell populations growing in a thermostated imaging...
This interdisciplinary work focuses on the interest of a new auto-encoder for supervised classification of live cell populations growing in a thermostated imaging station and acquired by a Quantitative Phase Imaging (QPI) camera. This type of camera produces interferograms that have to be processed to extract features derived from quantitative linear retardance and birefringence measurements. QPI is performed on living populations without any manipulation or treatment of the cells. We use the efficient new autoencoder classification method instead of the classical Douglas-Rachford method. Using this new supervised autoencoder, we show that the accuracy of the classification of the cells present in the mitotic phase of the cell cycle is very high using QPI features. This is a very important finding since we demonstrate that it is now possible to very precisely follow cell growth in a non-invasive manner, without any bias. No dye or any kind of markers are necessary for this live monitoring. Any studies requiring analysis of cell growth or cellular response to any treatment could benefit from this new approach by simply monitoring the proportion of cells entering mitosis in the studied cell population.
Topics: Algorithms; Cell Cycle; HeLa Cells; Humans; Image Processing, Computer-Assisted; Microscopy; Mitotic Index; Nocodazole; Supervised Machine Learning
PubMed: 34582352
DOI: 10.1109/TCBB.2021.3115876 -
Cell and Tissue Kinetics Jul 1976The influence of methotrexate (MTX) on the proliferative activity of cells in different phases of cell cycle has been studied. MTX (5 mg/kg) was injected i.p. 3 days...
The influence of methotrexate (MTX) on the proliferative activity of cells in different phases of cell cycle has been studied. MTX (5 mg/kg) was injected i.p. 3 days after the inoculation of 5 X 10(6) leukemia cells, into F1 (DBA X C57 BL) mice. It was shown that MTX causes degeneration of cells, being in G1- as well as in S-phase at the time of drug injection. Incorporation of 3H-TdR was suppressed for a period ranging from 2 to 12 hr after MTX administration, which is demonstrated by the decrease in the number of grains per cell. The number of cells labeled after 3H-TdR injection was also sharply decreased during this period. For a period of 3 until 15 hr after MTX administration the mitotic index decreased significantly as a result of inhibition of DNA synthesis. The blocking of the G1-S transition was evident during 4 hr after MTX. Thereafter the G1-S transition proceeds at a rate which is practically equal to that for nontreated controls. MTX did not inhibit transition to mitosis of cells being in G2-phase and in a very late S-phase at the time of drug injection. The sensitivity of G1-cells to the cytocidal effect of MTX shows that for L1210 leukemia cells MTX can be classified as a cycle-specific drug killing both G1-and S-cells rather than S-phase specific agent with self-limitation.
Topics: Animals; Cell Division; DNA, Neoplasm; Leukemia L1210; Methotrexate; Mitotic Index; Thymidine
PubMed: 945129
DOI: 10.1111/j.1365-2184.1976.tb01281.x -
Mutation Research Oct 1999In this study, cultures of human lymphocytes were exposed for 48 h to aqueous suspensions of Zinalco, with different times of corrosion, to test the biocompatibility of...
In this study, cultures of human lymphocytes were exposed for 48 h to aqueous suspensions of Zinalco, with different times of corrosion, to test the biocompatibility of this alloy and support its use in medical implants. The results show that the Zinalco concentrations assessed affect the mitotic index (MI) and some also increase the length of DNA migration measured in the comet assay in a significant manner; none of them showed clastogenic nor aneugenic effects. The authors conclude that Zinalco may be considered as an alternative biomaterial and suggest its use for short-term implants.
Topics: Adult; Alloys; Biocompatible Materials; Cells, Cultured; Comet Assay; DNA Damage; Female; Humans; Lymphocytes; Male; Materials Testing; Middle Aged; Mitotic Index
PubMed: 10613193
DOI: 10.1016/s1383-5718(99)00158-8 -
Environmental Toxicology and... Oct 2022The investigation aimed to determine the cytogenotoxic effect of fifth-generation quaternary ammonium using three plant species as bioindicators. Bulbs of A. cepa and...
The investigation aimed to determine the cytogenotoxic effect of fifth-generation quaternary ammonium using three plant species as bioindicators. Bulbs of A. cepa and seeds of L. culinaris and P. sativum were exposed to different concentrations of fifth-generation quaternary ammonium and a control solution of distilled water for 72 h. The results showed that the A. cepa bioindicator presented the greatest reduction in root length at 50 mg L and no mitotic index at 40 and 50 mg L, reaching 100% mitotic inhibition. Cell abnormalities were present among the three bioindicator species, where the highest index of micronuclei occurred at 50 mg L, being A. cepa the bioindicator with the highest relative rate of abnormality (25.28%). It was concluded that fifth-generation quaternary ammonium, in all treatments, caused a cytogenotoxic effect on the apical meristematic cells of the three species, A. cepa was the most sensitive species.
Topics: Ammonium Compounds; Chromosome Aberrations; Environmental Biomarkers; Mitotic Index; Plant Roots; Water
PubMed: 36089239
DOI: 10.1016/j.etap.2022.103972