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Journal of Surgical Oncology Dec 2017
Topics: Databases, Factual; Humans; Melanoma; Mitotic Index; Skin Neoplasms
PubMed: 28650585
DOI: 10.1002/jso.24738 -
Der Pathologe Nov 1995Oncocytomas are rare epithelial tumors of the major and minor salivary glands. Reported is an oncocytoma of the lateral wall of the left nasal cavity in a 60-year-old... (Review)
Review
Oncocytomas are rare epithelial tumors of the major and minor salivary glands. Reported is an oncocytoma of the lateral wall of the left nasal cavity in a 60-year-old man. Because of its locally invasive character, with rupture into the left nasal cavity and partial dedifferentiation with loss of typically oncocytic features, we classified the tumor as oncocytoma of low malignant potential.
Topics: Adenoma, Oxyphilic; Biomarkers, Tumor; Diagnosis, Differential; Humans; Immunoenzyme Techniques; Male; Middle Aged; Mitotic Index; Nasal Obstruction; Nose; Nose Neoplasms
PubMed: 8570564
DOI: 10.1007/s002920050126 -
International Journal of Surgical... Apr 2005Mitosis counting remains one of the most valuable prognostic indicators in tumor pathology; however, as currently carried out it is time consuming and not reproducible.... (Comparative Study)
Comparative Study
Mitosis counting remains one of the most valuable prognostic indicators in tumor pathology; however, as currently carried out it is time consuming and not reproducible. In this study, 6 different pathologists, using different microscopes, arrived at widely different mitotic counts on the same slide, ranging from 4 to 16. These differences were mainly due to the different field areas of the various microscopes used and the method used for counting and recording. In evaluating the most active 10 HPF, the count ranged from 10 to 19. Instead, when an average of 40 fields was recorded, the range was 4-11. Using the mitosis/volume index, which expresses the number of mitotic figures per mm2 of viable tumor, the counts ranged from 8 to 10, a marked improvement. However, this method is complicated and not "user-friendly.'' We suggest a variation of the technique by which a 2 mm2 rectangle is drawn on a cover slip and mounted under the microscope, centered on the most mitotically active area of the tumor. The mitoses in that area are counted (=n) and the percent of viable tumor (=x%) is estimated under low magnification. The number of mitoses per mm2 of viable tumor (cs-MAI) is then calculated according to the formula Cs-MAI=100n/2x. Using this modified method, the range of mitoses counted by the different observers was very narrow (9 to 11), and the time required for the counting was only 5-10 minutes.
Topics: Female; Humans; Leiomyoma; Microscopy; Mitotic Index; Observer Variation; Pathology, Surgical; Prognosis; Reproducibility of Results; Uterine Neoplasms
PubMed: 15864379
DOI: 10.1177/106689690501300206 -
Progress in Clinical and Biological... 1991
Review
Topics: Animals; Carcinogenicity Tests; Carcinogens; Cell Division; Formaldehyde; Humans; Mitotic Index; Respiratory System; Respiratory Tract Neoplasms
PubMed: 1946528
DOI: No ID Found -
Human Pathology Jul 1990
Topics: Humans; Mitosis; Mitotic Index; Neoplasms; Pathology, Clinical
PubMed: 2131787
DOI: 10.1016/0046-8177(90)90026-2 -
European Journal of Gynaecological... 2000Apoplectic leiomyoma is a distinctive smooth muscle tumour usually occurring in women either taking oral contraceptives or who are pregnant or recently postpartum. Most...
Apoplectic leiomyoma is a distinctive smooth muscle tumour usually occurring in women either taking oral contraceptives or who are pregnant or recently postpartum. Most of these tumours show 0-2 mitoses per 10 high power fields, but a mitotic index of up to 8 per 10 high power fields is allowed in such tumours. We describe an apoplectic leiomyoma with a number of atypical features including a high mitotic index (up to 20 per 10 high power fields) in a 47-year-old woman. Follow-up clinically and by computerised tomography (CT) for 3 years demonstrates no recurrence.
Topics: Female; Hemorrhage; Humans; Leiomyoma; Middle Aged; Mitotic Index; Prognosis; Smooth Muscle Tumor
PubMed: 10726614
DOI: No ID Found -
Annals of Diagnostic Pathology Dec 2013The number of mitotic figures in a predefined area is essential in pathologic evaluation for most tumors. This information sometimes provides clues in differentiating...
The number of mitotic figures in a predefined area is essential in pathologic evaluation for most tumors. This information sometimes provides clues in differentiating neoplastic lesions from nonneoplastic ones and sometimes in defining and grading of the tumors as well as prognosticating expected lifetime of the patient. As a generally accepted concept, scanning a certain number of consecutive nonoverlapping areas that are rich in viable tumor cells is required. Invasion fronts or the periphery of the tumors is preferred for counting mitosis. The target area to be counted for mitotic activity for various tumors is standardized as the number of mitosis in an established number of high-power fields. However, suggested mitotic counts, which constitute the basis of these studies, were obtained via the old microscopes, which usually had narrower visual fields than the state-of-the-art microscopes. Because the visual fields of the present microscopes provide larger areas compared with the older ones, corrections in mitosis counting are needed to make them compatible with the criteria, which had been put forward in the original reference studies.
Topics: Humans; Microscopy; Mitosis; Mitotic Index; Neoplasm Grading; Neoplasms; Pathology, Surgical; Prognosis; Reproducibility of Results; Visual Fields
PubMed: 23806202
DOI: 10.1016/j.anndiagpath.2013.05.005 -
Journal of Neuropathology and... Nov 1995Proliferative activity and DNA index were analyzed and correlated with histology and survival in 30 primary central nervous system lymphomas (PCNSL) in immunocompetent...
Proliferative activity and DNA index were analyzed and correlated with histology and survival in 30 primary central nervous system lymphomas (PCNSL) in immunocompetent patients. Proliferative activity was determined using mitotic activity index and volume-corrected mitotic index, percentage of Ki-67 (MIB-1) immunopositive nuclei and flow cytometric S-phase fraction. Twenty-nine PCNSL were of B-cell origin and one of T-cell; by Kiel classification there were 23% low grade and 77% high grade and by Working Formulation there were 7%, 73% and 20% low, intermediate and high grade tumors, respectively. Mean survival time for non-survivors (n = 26) was 11.5 months and median 6.5 months. When indicators of proliferative activity were evaluated against histological grading, correlation existed only between mitotic activity index and Kiel classification. None of the proliferation markers or DNA index correlated significantly with survival, but there was a trend for patients with higher volume-corrected mitotic index to have shorter survival. In conclusion, most PCNSL have poor prognosis irrespective of their histological grade and proliferative activity. Furthermore, because at present stereotactic biopsy is recommended for establishing the diagnosis, exact histological subtyping and determination of proliferation activity in such small samples appears to be of only marginal significance.
Topics: Adult; Aged; Cell Survival; Central Nervous System; DNA; Female; Flow Cytometry; Humans; Immunohistochemistry; Lymphoma; Male; Middle Aged; Mitotic Index; Prognosis; Time Factors
PubMed: 7595655
DOI: 10.1097/00005072-199511000-00009 -
Pathology, Research and Practice Sep 1996Twenty-one pathologists and technicians participated in a study evaluating the variation present in mitotic counts for prognostication of breast cancer. The participants...
Twenty-one pathologists and technicians participated in a study evaluating the variation present in mitotic counts for prognostication of breast cancer. The participants counted the mitotic figures in 20 breast cancer samples from ten high power fields (mitotic activity index, MAI, giving the results in mitotic figures per 10 fields) and also made a correction for field size and area fraction of the neoplastic epithelium to get the standardized mitotic index (volume fraction corrected mitotic index, or M/VV index, giving the result in mitotic figures per square mm of neoplastic epithelium). The difference in variation between the two methods was not big, but the standardized mitotic index (SMI) showed consistently smaller variation among all participants and different subgroups. Experienced pathologists had the highest variation in mitotic counts, and specially trained technicians, the lowest. The efficiency of the mitotic counts in grading (the grading efficiency) was used to evaluate the mitotic counts. In groups without special training for mitotic counts the mean grading efficiency was lower (experienced and training pathologists both on average had the potential to grade 88% of the cases correctly) than in the group specially trained for the purpose (trained technicians had the potential to grade 95% of the cases correctly). Among the specially trained technicians, the grading efficiency was of the same magnitude as the grading efficiency achieved in determining the S-Phase fraction of cells from paraffin embedded breast cancers by flow cytometry in different laboratories. The results suggest that special training is helpful in making mitotic counts more reproducible, and that in trained hands, the mitotic counts give results comparable to more sophisticated methods of determining proliferative activity in breast cancer.
Topics: Breast Neoplasms; Humans; Mitotic Index; Observer Variation; Pathology, Surgical; Reference Standards
PubMed: 8950760
DOI: 10.1016/S0344-0338(96)80075-6 -
Lancet (London, England) Aug 1981
Clinical Trial
Topics: Breast Neoplasms; Female; Humans; Mitosis; Mitotic Index; Prognosis
PubMed: 6114364
DOI: 10.1016/s0140-6736(81)90574-2