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Laboratory Animal Science Feb 1996A 16S rDNA-based polymerase chain reaction (PCR) method specific for Pasteurella pneumotropica was developed. The PCR product, a 395-base pair DNA fragment, was... (Comparative Study)
Comparative Study
A 16S rDNA-based polymerase chain reaction (PCR) method specific for Pasteurella pneumotropica was developed. The PCR product, a 395-base pair DNA fragment, was amplified from P. pneumotropica and not from 42 other bacterial species tested, including four other Pasteurella species and Actinobacillus ureae. The PCR method was used to identify 13 previously isolated strains that had been identified as P. pneumotropica by conventional methods: 12 were confirmed by PCR; one that was PCR-negative was re-examined by biochemical methods and determined to be A. ureae. The PCR detection of P. pneumotropica in nasopharyngeal swab specimens from 121 surveillance animals (15 inbred mice and 5 inbred rats from 20 animal rooms) had a high carrier state in healthy laboratory animals; for example, rat swab specimens were 89.6% (43/48) positive by PCR, 8.3% were positive by the direct culture-biochemical method, and 16.7% were positive by the enrichment culture-biochemical method. The positive rate for mice (21.9% [16/73]) was lower than that for rats.
Topics: Animals; Animals, Laboratory; Base Sequence; Carrier State; DNA, Bacterial; Electrophoresis, Agar Gel; Female; Male; Mice; Mice, Inbred Strains; Molecular Sequence Data; Pasteurella; Pasteurella Infections; Polymerase Chain Reaction; Predictive Value of Tests; Rats; Rats, Inbred Strains; Rodent Diseases; Species Specificity
PubMed: 8699827
DOI: No ID Found -
Zentralblatt Fur Bakteriologie :... Jun 1993A Pasteurella multocida adenylate cyclase gene has been previously cloned in Escherichia coli and sequenced. A 1200 bp HpaI fragment from the coding region was used as a...
A Pasteurella multocida adenylate cyclase gene has been previously cloned in Escherichia coli and sequenced. A 1200 bp HpaI fragment from the coding region was used as a probe to analyse the presence of the gene in different Pasteurella species and subspecies, Actinobacillus ureae (formerly P. ureae) and group EF-4 bacteria. Thirty-seven strains were checked for the presence of the gene. It was shown that the adenylate cyclase gene was detected only in the species Pasteurella multocida.
Topics: Adenylyl Cyclases; Blotting, Southern; DNA Probes; DNA, Bacterial; Genes, Bacterial; Pasteurella; Pasteurella multocida; Species Specificity
PubMed: 8369586
DOI: 10.1016/s0934-8840(11)80490-1