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International Journal of Environmental... Mar 2023Strength training in prepubertal children is one of the topics that has aroused the most interest and controversy among training professionals in recent years.... (Review)
Review
Strength training in prepubertal children is one of the topics that has aroused the most interest and controversy among training professionals in recent years. Therefore, the aim of the present study was to analyze the available scientific evidence on the influence of strength training variables on morphological and/or neuromuscular adaptations in healthy prepubertal populations with no previous experience in this type of training according to the descriptive sample characteristics. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis, 22 studies were selected after a systematic search and selection process using four electronic databases: Google Scholar, PubMed, Scopus, and SPORT Discus. Furthermore, the internal validity of the studies included was assessed using the modified PEDro scale. The sample consisted of 604 prepubertal children (age, 10.02 ± 0.75 years), of whom 473 were boys and 131 were girls, with 104 strength training programs recorded. Strength training resulted in a significant increase in jumping ( = 29) and sprinting ( = 13) abilities. Moreover, muscle strength was increased in 100% of the cases. Morphologically, strength training resulted in a decrease in body fat percentage ( = 19) and an increase in lean body mass ( = 17). With regard to gender, increases in general sport skills and basic physical abilities were significant in males but not in females. Thus, the results are more heterogeneous in girls due to the small number of studies carried out. Therefore, this research provides practical applications for coaches to design and implement more effective training programs to maximize adaptations, enhance physical performance, and reduce injury risk.
Topics: Male; Female; Humans; Child; Resistance Training; Muscle Strength; Sports; Adaptation, Physiological; Acclimatization
PubMed: 36981742
DOI: 10.3390/ijerph20064833 -
Proceedings of the National Academy of... Jun 2023As a crucial nitrogen source, nitrate (NO) is a key nutrient for plants. Accordingly, root systems adapt to maximize NO availability, a developmental regulation also...
As a crucial nitrogen source, nitrate (NO) is a key nutrient for plants. Accordingly, root systems adapt to maximize NO availability, a developmental regulation also involving the phytohormone auxin. Nonetheless, the molecular mechanisms underlying this regulation remain poorly understood. Here, we identify () in Arabidopsis (), whose root growth fails to adapt to low-NO conditions. is defective in the high-affinity NO transporter NRT2.1. () mutants exhibit defects in polar auxin transport, and their low-NO-induced root phenotype depends on the PIN7 auxin exporter activity. NRT2.1 directly associates with PIN7 and antagonizes PIN7-mediated auxin efflux depending on NO levels. These results reveal a mechanism by which NRT2.1 in response to NO limitation directly regulates auxin transport activity and, thus, root growth. This adaptive mechanism contributes to the root developmental plasticity to help plants cope with changes in NO availability.
Topics: Nitrate Transporters; Nitrates; Acclimatization; Biological Transport; Arabidopsis; Indoleacetic Acids
PubMed: 37307446
DOI: 10.1073/pnas.2221313120 -
Methods in Molecular Biology (Clifton,... 2023Biochemical networks are often characterized by tremendous complexity-both in terms of the sheer number of interacting molecules ("nodes") and in terms of the varied and... (Review)
Review
Biochemical networks are often characterized by tremendous complexity-both in terms of the sheer number of interacting molecules ("nodes") and in terms of the varied and incompletely understood interactions among these molecules ("interconnections" or "edges"). Strikingly, the vast and intricate networks of interacting proteins that exist within each living cell have the capacity to perform remarkably robustly, and reproducibly, despite significant variations in concentrations of the interacting components from one cell to the next and despite mutability over time of biochemical parameters. Here we consider the ubiquitously observed and fundamentally important signalling response known as robust perfect adaptation (RPA). We have recently shown that all RPA-capable networks, even the most complex ones, must satisfy an extremely rigid set of design principles, and are modular, being decomposable into just two types of network building-blocks-opposer modules and balancer modules. Here we present an overview of the design principles that characterize all RPA-capable network topologies through a detailed examination of a collection of simple examples. We also introduce a diagrammatic method for studying the potential of a network to exhibit RPA, which may be applied without a detailed knowledge of the complex mathematical principles governing RPA.
Topics: Signal Transduction; Adaptation, Physiological; Acclimatization; Models, Biological
PubMed: 37074572
DOI: 10.1007/978-1-0716-3008-2_1 -
Cell Systems Jun 2021A distinctive feature of many biological systems is their ability to adapt to persistent stimuli or disturbances that would otherwise drive them away from a desirable... (Review)
Review
A distinctive feature of many biological systems is their ability to adapt to persistent stimuli or disturbances that would otherwise drive them away from a desirable steady state. The resulting stasis enables organisms to function reliably while being subjected to very different external environments. This perspective concerns a stringent type of biological adaptation, robust perfect adaptation (RPA), that is resilient to certain network and parameter perturbations. As in engineered control systems, RPA requires that the regulating network satisfy certain structural constraints that cannot be avoided. We elucidate these ideas using biological examples from systems and synthetic biology. We then argue that understanding the structural constraints underlying RPA allows us to look past implementation details and offers a compelling means to unravel regulatory biological complexity.
Topics: Acclimatization; Adaptation, Physiological; Homeostasis; Models, Biological; Synthetic Biology
PubMed: 34139163
DOI: 10.1016/j.cels.2021.05.020 -
International Journal of Molecular... Apr 2022Plants have remarkable plasticity due to their vast genetic potential which interacts with many external factors and developmental signals to govern development and...
Plants have remarkable plasticity due to their vast genetic potential which interacts with many external factors and developmental signals to govern development and adaptation to changing environments [...].
Topics: Acclimatization; Adaptation, Physiological; Plant Development; Plants; Systems Biology
PubMed: 35456977
DOI: 10.3390/ijms23084159 -
Global Change Biology May 2021Marine microalgae are a diverse group of microscopic eukaryotic and prokaryotic organisms capable of photosynthesis. They are important primary producers and carbon... (Review)
Review
Marine microalgae are a diverse group of microscopic eukaryotic and prokaryotic organisms capable of photosynthesis. They are important primary producers and carbon sinks but their physiology and persistence are severely affected by global climate change. Powerful experimental evolution technologies are being used to examine the potential of microalgae to respond adaptively to current and predicted future conditions, as well as to develop resources to facilitate species conservation and restoration of ecosystem functions. This review synthesizes findings and insights from experimental evolution studies of marine microalgae in response to elevated temperature and/or pCO . Adaptation to these environmental conditions has been observed in many studies of marine dinoflagellates, diatoms and coccolithophores. An enhancement in traits such as growth and photo-physiological performance and an increase in upper thermal limit have been shown to be possible, although the extent and rate of change differ between microalgal taxa. Studies employing multiple monoclonal replicates showed variation in responses among replicates and revealed the stochasticity of mutations. The work to date is already providing valuable information on species' climate sensitivity or resilience to managers and policymakers but extrapolating these insights to ecosystem- and community-level impacts continues to be a challenge. We recommend future work should include in situ experiments, diurnal and seasonal fluctuations, multiple drivers and multiple starting genotypes. Fitness trade-offs, stable versus plastic responses and the genetic bases of the changes also need investigating, and the incorporation of genome resequencing into experimental designs will be invaluable.
Topics: Acclimatization; Climate Change; Ecosystem; Microalgae; Oceans and Seas
PubMed: 33547698
DOI: 10.1111/gcb.15546 -
Journal of Thermal Biology Aug 2023Given the increasing trend of global warming and extreme weather conditions, including heat waves and its effects on health, the present study was done to investigate... (Review)
Review
BACKGROUND
Given the increasing trend of global warming and extreme weather conditions, including heat waves and its effects on health, the present study was done to investigate adaptive behaviors of communities in the world for combating heat waves.
METHOD
ology: In this systematic review, out of 1529 results, 57 relevant and authoritative English papers on adaptation to heat waves hazard were extracted and evaluated using valid keywords from valid databases (PubMed, WOS, EMBASE, and Scopus). In addition, multiple screening steps were done and then, the selected papers were qualitatively assessed. Evaluation results were summarized using an Extraction Table.
RESULTS
In this paper, the adaptive behaviors for combating heat waves hazard were summarized into 11 categories: Education and awareness raising, Adaptation of critical infrastructure, Governments measures, Health-related measures, Application of early warning system, Protective behaviors in workplace, Physical condition, Adaptive individual behaviors, Design and architecture of the building, Green infrastructure (green cover), and Urban design.
CONCLUSION
The findings of this study showed that community actions have significant effects on adaptation to heat wave. Therefore, for reducing heat wave-related negative health effects and vulnerability, more attention should be paid to the above-mentioned actions for mitigation, preparation, and responding regarding heat waves.
PROSPERO REGISTRATION NUMBER
CRD42021257747.
Topics: Hot Temperature; Acclimatization; Adaptation, Physiological; Global Warming; Adaptation, Psychological; Climate Change
PubMed: 37499408
DOI: 10.1016/j.jtherbio.2023.103588 -
Journal of Visualized Experiments : JoVE Jan 2023Festa, F., Labaer, J. Kinase inhibitor screening in self-assembled human protein microarrays. Journal of Visualized Experiments. 152, e59886 (2019). Stockman, B. J. et...
Festa, F., Labaer, J. Kinase inhibitor screening in self-assembled human protein microarrays. Journal of Visualized Experiments. 152, e59886 (2019). Stockman, B. J. et al. NMR-Based activity assays for determining compound inhibition, IC50 values, artifactual activity, and whole-cell activity of nucleoside ribohydrolases. Journal of Visualized Experiments. 148, e59928 (2019). Gao, S. et al. A high-throughput assay for the prediction of chemical toxicity by automated phenotypic profiling of Caenorhabditis elegans. Journal of Visualized Experiments. 145, e59082 (2019). Axelsson, H., Almqvist, H., Seashore-Ludlow, B. Using high content imaging to quantify target engagement in adherent cells. Journal of Visualized Experiments. 141, e58670 (2018). Chorba, J. S., Galvan, A. M., Shokat, K. M. A high-throughput luciferase assay to evaluate proteolysis of the single-turnover protease PCSK9. Journal of Visualized Experiments. 138, e58265 (2018). Sullivan, C. et al. Using zebrafish models of human influenza A virus infections to screen antiviral drugs and characterize host immune cell responses. Journal of Visualized Experiments. 119, e55235 (2017). Tiemann, K., Garri, C., Wang, J., Clarke, L., Kani, K. Assessment of resistance to tyrosine kinase inhibitors by an interrogation of signal transduction pathways by antibody arrays. Journal of Visualized Experiments. 139, e57779 (2018). Radnai, L., Stremel, R. F., Sellers, J. R., Rumbaugh, G., Miller, C. A. A semi-high-throughput adaptation of the NADH-coupled ATPase assay for screening small molecule inhibitors. Journal of Visualized Experiments. 150, e60017 (2019). Nandha Premnath, P., Craig, S., McInnes, C. Development of inhibitors of protein-protein interactions through REPLACE: Application to the design and development non-ATP competitive CDK inhibitors. Journal of Visualized Experiments. 104, e52441 (2015). Chen, E. W., Ke, C. Y., Brzostek, J., Gascoigne, N. R. J., Rybakin, V. Identification of mediators of T-cell receptor signaling via the screening of chemical inhibitor libraries. Journal of Visualized Experiments. 143, e58946 (2019). Takakusagi, Y. Biosensor-based high throughput biopanning and bioinformatics analysis strategy for the global validation of drug-protein interactions. Journal of Visualized Experiments. 166, e61873 (2020). Riching, K. M., Mahan, S. D., Urh, M., Daniels, D. L. High-Throughput cellular profiling of targeted protein degradation compounds using HiBiT CRISPR cell lines. Journal of Visualized Experiments. 165, e61787 (2020).
Topics: Humans; Animals; Proprotein Convertase 9; Zebrafish; Proteolysis; Acclimatization; Caenorhabditis elegans
PubMed: 37602881
DOI: 10.3791/64811 -
International Journal of Molecular... Oct 2019Necessitated by the subzero temperatures and seasonal exposure to ice, various organisms have developed a remarkably effective means to survive the harsh climate of... (Review)
Review
Necessitated by the subzero temperatures and seasonal exposure to ice, various organisms have developed a remarkably effective means to survive the harsh climate of their natural habitats. Their ice-binding (glyco)proteins keep the nucleation and growth of ice crystals in check by recognizing and binding to specific ice crystal faces, which arrests further ice growth and inhibits ice recrystallization (IRI). Inspired by the success of this adaptive strategy, various approaches have been proposed over the past decades to engineer materials that harness these cryoprotective features. In this review we discuss the prospects and challenges associated with these advances focusing in particular on peptidic antifreeze materials both identical and akin to natural ice-binding proteins (IBPs). We address the latest advances in their design, synthesis, characterization and application in preservation of biologics and foods. Particular attention is devoted to insights in structure-activity relations culminating in the synthesis of de novo peptide analogues. These are sequences that resemble but are not identical to naturally occurring IBPs. We also draw attention to impactful developments in solid-phase peptide synthesis and 'greener' synthesis routes, which may aid to overcome one of the major bottlenecks in the translation of this technology: unavailability of large quantities of low-cost antifreeze materials with excellent IRI activity at (sub)micromolar concentrations.
Topics: Acclimatization; Antifreeze Proteins; Biomimetics; Cryoprotective Agents; Crystallization; Freezing; Ice
PubMed: 31627404
DOI: 10.3390/ijms20205149 -
Proceedings. Biological Sciences Feb 2023Conflicts of interest abound not only in human affairs but also in the biological realm. Evolutionary conflict occurs over multiple scales of biological organization,...
Conflicts of interest abound not only in human affairs but also in the biological realm. Evolutionary conflict occurs over multiple scales of biological organization, from genetic outlawry within genomes, to sibling rivalry within nuclear families, to collective-action disputes within societies. However, achieving a general understanding of the dynamics and consequences of evolutionary conflict remains an outstanding challenge. Here, we show that a development of R. A. Fisher's classic 'geometric model' of adaptation yields novel and surprising insights into the dynamics of evolutionary conflict and resulting maladaptation, including the discoveries that: (i) conflict can drive evolving traits arbitrarily far away from all parties' optima and, indeed, if all mutations are equally likely then contested traits are more often than not driven outwith the zone of actual conflict (hyper-maladaptation); (ii) evolutionary conflicts drive persistent maladaptation of orthogonal, non-contested traits (para-maladaptation); and (iii) modular design greatly ameliorates conflict-driven maladaptation, thereby facilitating major transitions in individuality.
Topics: Humans; Biological Evolution; Adaptation, Physiological; Mutation; Genome; Acclimatization
PubMed: 36750194
DOI: 10.1098/rspb.2022.2423