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Annals of Tropical Medicine and... Oct 1998Sera from 82 cases of leptospirosis (confirmed by micro-agglutination tests or IFAT) and 108 patients with other diseases were investigated using the microcapsule...
Sera from 82 cases of leptospirosis (confirmed by micro-agglutination tests or IFAT) and 108 patients with other diseases were investigated using the microcapsule agglutination test (MCAT) for leptospirosis. The overall sensitivity (90.2%), specificity (96.3%), positive predictive value (94.9%), negative predictive value (92.9%), and accuracy of the MCAT (93.7%) were encouraging. MCAT is simple, can be performed by unskilled personnel with minimum laboratory facilities, and produces results in 3 h. MCAT would be a reliable serodiagnostic test for rapidly screening individuals for leptospirosis, in various geographical areas of Thailand.
Topics: Agglutination Tests; Fever; Fluorescent Antibody Technique, Indirect; Humans; Leptospira interrogans; Leptospirosis; Sensitivity and Specificity; Thailand
PubMed: 9924538
DOI: 10.1080/00034989859041 -
The Cornell Veterinarian Apr 1955
Topics: Agglutination; Agglutination Tests; Animals; Humans; Leptospirosis; Milk
PubMed: 14365180
DOI: No ID Found -
Bulletin of the Calcutta School of... Oct 1963
Topics: Agglutination; Agglutination Tests; Cholera; Humans
PubMed: 14120864
DOI: No ID Found -
Tropical Animal Health and Production Aug 1996A spot agglutination test was standardised for the rapid diagnosis of goat pox using antigen and antibody systems after being coupled to glutaraldehyde-treated sheep... (Comparative Study)
Comparative Study
A spot agglutination test was standardised for the rapid diagnosis of goat pox using antigen and antibody systems after being coupled to glutaraldehyde-treated sheep erythrocytes. The sensitivity was comparable to that of counter-immunoelectrophoresis and reverse-phase passive haemagglutination tests. The spot agglutination test was found to be very simple, easy to perform, rapid and economical for the detection of goat pox antigen and antibody and can be performed under field conditions.
Topics: Agglutination Tests; Animals; Antibodies, Viral; Antigens, Viral; Counterimmunoelectrophoresis; Erythrocytes; Goat Diseases; Goats; Hemagglutination Tests; Poxviridae Infections; Sensitivity and Specificity; Sheep
PubMed: 8888526
DOI: 10.1007/BF02240937 -
Medical Laboratory Sciences Apr 1991The performance of a slide agglutination test for detection of toxoplasma-specific IgG was assessed and compared with that of the dye test and latex agglutination test.... (Comparative Study)
Comparative Study
The performance of a slide agglutination test for detection of toxoplasma-specific IgG was assessed and compared with that of the dye test and latex agglutination test. The slide agglutination test was as sensitive as, and more specific than, latex agglutination. The predictive value of a negative slide agglutination test was less than the latex agglutination test but produced results within minutes, although quantitative results were not comparable to other assays. Slide agglutination presents a rapid alternative to the latex agglutination test as a screening assay for toxoplasmosis, although patients at risk of life-threatening infection require detailed serological examination using additional methods.
Topics: Agglutination Tests; Animals; Antibodies, Protozoan; Female; Humans; Immunoglobulin G; Latex; Pregnancy; Pregnancy Complications, Infectious; Toxoplasma; Toxoplasmosis
PubMed: 1943540
DOI: No ID Found -
The American Journal of Tropical... May 2016A prerequisite for the control of visceral leishmaniasis (VL) is the accessibility to reference diagnostics. The high price of the freeze-dried direct agglutination test...
A prerequisite for the control of visceral leishmaniasis (VL) is the accessibility to reference diagnostics. The high price of the freeze-dried direct agglutination test (FD-DAT) and the short shelf-life time of the rK39 strip test (rK39) have limited the application of these tests in Sudan. An original liquid DAT (LQ-DAT) with high reproducibility compared with the FD-DAT and rK39 has been routinely produced in our laboratory since 1999. In this study, a 3.4-year-old batch (of more than 90 test batches produced to date) was chosen to validate the diagnostic performance of this test against microscopy, FD-DAT, and rK39 in 96 VL and 42 non-VL serum samples. Relatively higher sensitivity (95/96, 99.0%) was recorded for the LQ-DAT than for the FD-DAT (92/96, 95.8%) and rK39 (76/96, 79.2%), probably because of the use of the endemic autochthonous Leishmania donovani isolate as the antigen. Experience with the LQ-DAT, its low cost of production, ease of providing this test, and diagnostic reliability compared with the FD-DAT suggest that widescale implementation of the LQ-DAT can contribute to sustainable VL control in Sudan.
Topics: Agglutination Tests; Humans; Leishmaniasis, Visceral; Reproducibility of Results; Sensitivity and Specificity; Sudan
PubMed: 26976890
DOI: 10.4269/ajtmh.15-0574 -
Journal of Wildlife Diseases Jul 1976Inoculation of susceptible calves confirmed that the modified card agglutination test accurately detected the anaplasmosis infection status of each of 35 Columbian...
Inoculation of susceptible calves confirmed that the modified card agglutination test accurately detected the anaplasmosis infection status of each of 35 Columbian black-tailed deer (Odocoileus hemionus columbianus). Anaplasma marginale, and specific antibodies, were demonstrated only in calves which received blood from deer that were positive by the card test. The modified card agglutination testing of deer serum was performed in the manner recommended for testing cattle serum with bovine-origin antigen and bovine serum factor.
Topics: Agglutination Tests; Anaplasmosis; Animals; Animals, Wild; California; Deer; Sensitivity and Specificity; Seroepidemiologic Studies
PubMed: 16498890
DOI: 10.7589/0090-3558-12.3.427 -
Journal of Clinical Microbiology Dec 1975A new microtechnique has been developed for the detection of leptospiral antibodies in serum by the microscopic agglutination test. The test was set up in a microtiter... (Comparative Study)
Comparative Study
A new microtechnique has been developed for the detection of leptospiral antibodies in serum by the microscopic agglutination test. The test was set up in a microtiter transfer plate held in a transfer plate holder, resting on a transfer plate cover. Live leptospiral antigen was added and a second transfer plate cover was placed over the transfer plate during 2 h of incubation at 32 C. After incubation the bottom cover was removed and the complete unit was placed in a specially designed base plate containing microscope slides (50 by 75 mm). The serum/antigen mixture was ejected on to the microscope slides by means of a sharp tap. The agglutination was then read using a 10 X objective, 10 X eyepieces, and a dry, dark field condenser.
Topics: Agglutination Tests; Antibodies, Bacterial; Antigens, Bacterial; Diagnosis, Differential; Evaluation Studies as Topic; Humans; Leptospira; Leptospirosis
PubMed: 1206106
DOI: 10.1128/jcm.2.6.474-477.1975 -
Transactions of the Royal Society of... 1993The direct agglutination test (DAT) has been assessed as a diagnostic procedure for visceral leishmaniasis. Fifty-six of 58 sera (96.5%) from confirmed cases of visceral...
The direct agglutination test (DAT) has been assessed as a diagnostic procedure for visceral leishmaniasis. Fifty-six of 58 sera (96.5%) from confirmed cases of visceral leishmaniasis, whose bone marrow aspirates contained Leishmania donovani amastigotes, had agglutinating antibodies above the cut-off titre of 1:800. None of the sera from healthy control subjects from non-endemic or endemic areas had anti-leishmanial antibodies. Similarly, none of the sera obtained from cases of malaria or tuberculosis had agglutinating antibodies above the cut-off titre. A significant decline in agglutinating antibody titre in 3 cases following antileishmanial chemotherapy appeared to correlate with regression of clinical symptoms and the absence of amastigotes from bone marrow aspirates. One of 3 cases developed post-kala-azar dermal lesions and sera from this subject had an elevated agglutinating antibody titre. It is concluded that the DAT is a sensitive and specific test to confirm visceral leishmaniasis. As the formalin-fixed promastigotes, stained with Coomassie blue, which are used as antigen could be stored at 4 degrees C for 6 months without any loss of ability to detect anti-leishmanial antibodies, the DAT is recommended for use under field conditions.
Topics: Agglutination Tests; Animals; Antibodies, Helminth; Humans; Leishmania donovani; Leishmaniasis, Visceral; Sensitivity and Specificity
PubMed: 8236390
DOI: 10.1016/0035-9203(93)90125-a -
Acta Veterinaria Scandinavica Feb 2012The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA).
BACKGROUND
The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA).
METHODS
Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA.
RESULTS
The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA.
CONCLUSIONS
The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT.
Topics: Agglutination Tests; Anemia, Hemolytic, Autoimmune; Animals; Cat Diseases; Cats; Dog Diseases; Dogs; Gels
PubMed: 22316049
DOI: 10.1186/1751-0147-54-10