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Journal of the Medical Association of... Jun 1983
Topics: Adult; Agglutination Tests; Carrier State; Humans; Typhoid Fever
PubMed: 6631294
DOI: No ID Found -
Journal of Veterinary Diagnostic... Jul 2007The Rose Bengal plate agglutination test (RBT) was evaluated for the diagnosis of equine glanders, and its diagnostic efficiency was compared with that of mallein and... (Comparative Study)
Comparative Study
The Rose Bengal plate agglutination test (RBT) was evaluated for the diagnosis of equine glanders, and its diagnostic efficiency was compared with that of mallein and other serological tests, including indirect hemagglutination test (IHAT), complement fixation test (CFT), and modified counter immunoelectrophoresis test (mCIET). Sera from 70 naturally infected culture-positive, 96 potentially exposed cohorts, and 110 healthy equines were tested. All tests but mCIET showed 100% specificity when testing the sera from glanders-negative equines. The calculated sensitivities of RBT, IHAT, CFT, mCIET, and mallein test when testing culture-positive equines were 90.0, 97.1, 91.4, 81.4, and 75.7%, respectively. The RBT was significantly (P < 0.05) more sensitive than the mallein test and mCIET. The positive and negative predictive values of each test (RBT, IHAT, CFT, mallein test, and mCIET) were as follows: 100 and 94, 100 and 98.2, 100 and 96.7, 100 and 86.6, and 90.5 and 88.6, respectively. On comparing glandered and nonglandered animals, the highest agreement (0.987) was found between RBT and CFT followed by RBT and IHAT (0.940), RBT and mallein test (0.871), and RBT and mCIET (0.852). Because the RBT is simpler and rapid to perform, the inclusion of the test as a supplementary test for the diagnosis of glanders in field conditions is recommended.
Topics: Agglutination Tests; Animals; Glanders; Horses; Rose Bengal; Serologic Tests
PubMed: 17609344
DOI: 10.1177/104063870701900404 -
Bulletin of the World Health... 1971The systematics of the Leptospira is based on the agglutination test and on the cross-agglutination-adsorption test. To obtain reproducible and comparable results these...
The systematics of the Leptospira is based on the agglutination test and on the cross-agglutination-adsorption test. To obtain reproducible and comparable results these tests must be carried out by standard techniques, but the accuracy of the tests is in any case limited since immune sera prepared from animals do not always react uniformly. Moreover, the micro-organisms used in the tests have specific requirements and do not always develop properly in synthetic media. The various factors that influence the results of agglutination-adsorption tests were investigated and evaluated. Taking the results of these studies into account, a standardized method for agglutination-adsorption tests is proposed and described.
Topics: Adsorption; Agglutination Tests; Leptospira; Methods; Serotyping
PubMed: 5317449
DOI: No ID Found -
Revista Chilena de Obstetricia Y... 1969
Topics: Agglutination Tests; Female; Humans; Infertility, Female; Male; Methods; Spermatozoa
PubMed: 5385460
DOI: No ID Found -
The Journal of Allergy Jun 1966
Topics: Agglutination Tests; Animals; Coombs Test; Humans; Hypersensitivity; Immune Sera; In Vitro Techniques; Pollen; Rabbits; Serologic Tests
PubMed: 5221214
DOI: 10.1016/0021-8707(66)90132-8 -
Journal of Postgraduate Medicine 1993Coagglutination test was compared with commercially available latex agglutination test (Rotalex kit) for detection of rota virus in faecal samples from clinically...
Coagglutination test was compared with commercially available latex agglutination test (Rotalex kit) for detection of rota virus in faecal samples from clinically suspected cases of viral gastroenteritis. Out of 80 test samples 16 (20%) and 20 (25.3%) were positive for rota virus antigen by Rotalex kit and coagglutination test respectively. All the 40 controls were negative for viral antigen by Rotalex kit and only one gave positive result by coagglutination test. Coagglutination test was found to be economical, sensitive and specific for screening and rapid diagnosis of Rota virus diarrhoea.
Topics: Agglutination Tests; Child; Child, Preschool; Diarrhea; Feces; Female; Gastroenteritis; Humans; Infant; Latex Fixation Tests; Male; Reagent Kits, Diagnostic; Rotavirus; Rotavirus Infections
PubMed: 8051640
DOI: No ID Found -
Journal of Clinical Microbiology Feb 1982A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of...
A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2 min. Clinical studies showed 100% agreement between this method and the conventional 2-mercaptoethanol tube agglutination test. Excellent correlation was shown between cultural isolation and the modified rapid slide agglutination test, using sera from experimentally infected dogs.
Topics: Agglutination Tests; Animals; Brucellosis; Dog Diseases; Dogs; Serologic Tests
PubMed: 7068825
DOI: 10.1128/jcm.15.2.286-289.1982 -
Bulletin of the World Health... 1977This study presents the results of a number of serological tests, in particular the plate agglutination test using acid antigen stained with rose bengal, in the... (Comparative Study)
Comparative Study
This study presents the results of a number of serological tests, in particular the plate agglutination test using acid antigen stained with rose bengal, in the examination of 440 human sera for brucellosis. In addition, the sera were examined for the presence of specific antibodies of various classes (IgM and IgG). These investigations showed the rose bengal plate agglutination test to be highly specific and sensitive as a rapid method for the diagnosis of human brucellosis.
Topics: Agglutination Tests; Antigens, Bacterial; Brucella; Brucellosis; Coombs Test; Cysteine; Hemagglutination Tests; Humans; Immunoglobulin G; Immunoglobulin M; Rose Bengal
PubMed: 304394
DOI: No ID Found -
Revue Scientifique Et Technique... Dec 2012In this study, 241 serum samples from individuals exposed to brucellosis were subjected to the Rose Bengal plate test (RBPT); the titre was estimated by standard tube... (Comparative Study)
Comparative Study
In this study, 241 serum samples from individuals exposed to brucellosis were subjected to the Rose Bengal plate test (RBPT); the titre was estimated by standard tube agglutination test (STAT), with positive > or = 80 IU/ml. Randomly selected sera (n = 81) were analysed by complement fixation test (CFT): titre > or = 1:4 was considered positive. Of 241 sera subjected to RBPT and STAT, 177 were negative in both tests; 5 samples tested negative by RBPT but positive by STAT. None was positive by RBPT and negative by STAT. Of 81 sera subjected to CFT, 23 (28.4%) were positive. Both RBPT and CFT found 18 samples positive; 5 samples were positive by CFT and negative by RBPT. Comparison of STAT with CFT showed 13 samples positive by STAT but negative by CFT, and 4 positive by CFT but negative by STAT. The sensitivity and specificity of STAT were 82.6% and 77.6%, respectively, with CFT as gold standard. No test is perfect, and the clinical history coupled with a combination of two or more tests will reduce diagnostic errors.
Topics: Agglutination Tests; Antibodies, Bacterial; Brucella; Brucellosis; Complement Fixation Tests; Cross-Sectional Studies; Fluorescent Dyes; Humans; Rose Bengal; Sensitivity and Specificity
PubMed: 23520751
DOI: 10.20506/rst.31.3.2175 -
Genitourinary Medicine Aug 1988A comparative evaluation of a new serological test for human immunodeficiency virus (HIV) was carried out. The test used the agglutination of gelatin particles coated... (Comparative Study)
Comparative Study
A comparative evaluation of a new serological test for human immunodeficiency virus (HIV) was carried out. The test used the agglutination of gelatin particles coated with HIV antigen. It was found to be more sensitive than current enzyme linked immunosorbent assays (ELISAs) and more specific than the western blot test. Because of its simplicity, it promises to be of value, especially in developing countries.
Topics: Agglutination Tests; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; HIV Antibodies; HIV Seropositivity; Humans; Immunoenzyme Techniques; Reagent Kits, Diagnostic
PubMed: 3049303
DOI: 10.1136/sti.64.4.266