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Le Infezioni in Medicina Dec 2018New identification techniques such as gene sequencing and mass spectrometry have increased the incidence of novel agents such as Kerstersia gyiorum. As a new member of... (Review)
Review
New identification techniques such as gene sequencing and mass spectrometry have increased the incidence of novel agents such as Kerstersia gyiorum. As a new member of the Alcaligenaceae family, K. gyiorum was isolated from wounds, respiratory tract, urine specimens and most frequently from chronic suppurative otitis media (CSOM). We isolated three K. gyiorum strains from three CSOM cases over a one-year period. The strains were analyzed by mass spectrometry and identified by Bruker Biotyper 3.1 (Bruker Daltonics, USA). The cases were young patients without chronic diseases and immunodeficiencies. Two strains were resistant to ciprofloxacin.
Topics: Adult; Alcaligenaceae; Chronic Disease; Female; Gram-Negative Bacterial Infections; Humans; Male; Otitis Media, Suppurative; Young Adult
PubMed: 30555142
DOI: No ID Found -
Journal of Microbiology (Seoul, Korea) Feb 2020An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119, was isolated from human...
An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119, was isolated from human faeces from a Korean. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was a member of the genus Sutterella and most closely related to Sutterlla wadsworthensis KCTC 15691 (96.8% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB03119 was 58.3 mol% as determined from its whole genome sequence. Strain KGMB03119 was asaccharolytic, catalase-positive, oxidase- and urease-negative. Furthermore, the isolate was positive for alkaline phosphatase, leucine arylamidase, acid phosphatase, arginine arylamidase, alanine arylamidase, and glycine arylamidase. The major cellular fatty acids (> 10%) of the isolate were Cω9c and C. Methylmenaquinone-5 (MMK-5, 100%) was the predominant isoprenoid quinone in the isolate. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain KGMB03119 represents a novel species, for which the name Sutterella faecalis sp. nov. is proposed. The type strain is KGMB03119 (= KCTC 15823 = NBRC 114254).
Topics: Alcaligenaceae; Classification; DNA, Bacterial; Feces; Gastrointestinal Microbiome; Humans; Phylogeny; RNA, Ribosomal, 16S
PubMed: 31993985
DOI: 10.1007/s12275-020-9396-9 -
Journal of Hazardous Materials Apr 2021Microbial fuel cells (MFCs) are capable of removing tetracycline in soils, in which the degradation efficiency of tetracycline is hindered by its strong adsorption...
Microbial fuel cells (MFCs) are capable of removing tetracycline in soils, in which the degradation efficiency of tetracycline is hindered by its strong adsorption capacity. Phosphate was chosen as a competitor for tetracycline adsorption to improve its removal rate in soil MFCs. The results showed that 42-50% of tetracycline was degraded within 7 days, which was 42-67% higher than open-circuit treatments. Compared with closed-circuit treatments without phosphate addition, the removal efficiencies of tetracycline after phosphate addition increased by 19-25% on day 51, and accumulated charge outputs were enhanced by 31-52%, while the abundance of antibiotic resistance genes decreased by 19-27%. Like Geobacter, the abundance of Desulfurispora and Anaeroomyxobacter in the anode showed similar tendencies with current densities, suggesting their dominant roles in bioelectricity generation. Gemmatimonadetes bacterium SCN 70-22, Azohydromonas australica, Steroidobacter denitrificans and Gemmatirosa kalamazoonesis were found to be potential electrotrophic microbes in the cathode. The expressed flavoprotein 2,3-oxidoreductase, quinol oxidase and fumarate reductase might have promoted the transfer efficiency of electrons from cathodes to cells, which finally accelerated the biodegradation rate of tetracycline in addition to the polyphenol oxidase. This study provides an insight into functional enzyme genes in the soil microbial electrochemical remediation.
Topics: Alcaligenaceae; Bacteria; Bioelectric Energy Sources; Electrodes; Gammaproteobacteria; Soil; Soil Microbiology; Tetracycline
PubMed: 33388628
DOI: 10.1016/j.jhazmat.2020.124880 -
Proceedings. Biological Sciences Jul 2006Apparent competition between species is believed to be one of the principal driving forces that structure ecological communities, although the precise mechanisms have...
Apparent competition between species is believed to be one of the principal driving forces that structure ecological communities, although the precise mechanisms have yet to be characterized. Here we develop a model system that isolates phage-mediated interactions by neutralizing resource competition with a large excess of nutrients, and consists of two genetically identical Bordetella strains that differ only in that one is the carrier of phage and the other is susceptible to the phage. We observe and quantify the competitive advantage of the bacterial strain bearing the prophage in both invading and in resisting invasion by the bacterial strain sensitive to the phage, and use our experimental measurements to develop a mathematical model of phage-mediated competition. The model predicts, and experimental evidence confirms, that the competitive advantage conferred by the lysogenic phage depends only on the phage pathology on the sensitive bacterial strain and is independent of other phage and host parameters, such as the infection-causing contact rate, the spontaneous and infection-induced lysis rates and the phage burst size. This work combines experimental and mathematical approaches to the study of phage-driven competition, and provides an experimentally tested framework for evaluation of the effects of pathogens/parasites on interspecific competition.
Topics: Bacteriophages; Bordetella; Lysogeny; Models, Biological
PubMed: 16790419
DOI: 10.1098/rspb.2006.3512 -
The Journal of International Medical... May 2023With the development of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal RNA (rRNA) gene sequencing,... (Review)
Review
With the development of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal RNA (rRNA) gene sequencing, increasing numbers of new microorganisms are being discovered. In this report, was isolated for the first time from the sputum of two elderly patients with neurodegenerative disease, and integrated traditional Chinese and Western medicine was used for treatment. The bacteria's growth characteristics, biochemical reaction characteristics, sensitivity to antibiotics, and the patients' treatment are described, with a review of previous reports.
Topics: Aged; Humans; Neurodegenerative Diseases; Alcaligenaceae; Anti-Bacterial Agents; Language
PubMed: 37161265
DOI: 10.1177/03000605231171009 -
Journal of Infection in Developing... Jun 2014Oligella species are small, Gram-negative, nonsaccharolytic aerobic rods or coccobacilli that are catalase and oxidase-positive, mostly isolated from the urinary tract... (Review)
Review
Oligella species are small, Gram-negative, nonsaccharolytic aerobic rods or coccobacilli that are catalase and oxidase-positive, mostly isolated from the urinary tract and rarely from wounds, bloodstream infections, septic arthritis, or peritonitis.In this article, we report a case of O.ureolytica-related bloodstream infection in a newborn infant and we review the literature for previously reported cases of Oligella infections.
Topics: Alcaligenaceae; Anti-Bacterial Agents; Bacteremia; Developing Countries; Female; Gram-Negative Bacterial Infections; Humans; Infant, Newborn; Microbial Sensitivity Tests; Netilmicin; Turkey
PubMed: 24916881
DOI: 10.3855/jidc.3260 -
American Journal of Respiratory and... Oct 1996Bordetella species are respiratory pathogens that infect humans and other animals. A majority of the virulence factors expressed by these bacteria are regulated by a... (Review)
Review
Bordetella species are respiratory pathogens that infect humans and other animals. A majority of the virulence factors expressed by these bacteria are regulated by a master control locus, BvgAS, a member of the two-component family of signal transduction systems. BvgAS senses environmental signals and mediates signal transduction by a phosphorylation cascade that leads to a biphasic transition between the Bvg+ and Bvg- phases. From natural host studies using Bordetella bronchiseptica, we have found that expression of Bvg+ phase factors, which include adhesins and toxins, is required for successful colonization of the mammalian respiratory tract. Suppression of the Bvg- phase motility phenotype is necessary for a successful interaction with the host. Although the Bvg- phase does not appear to be required in vivo, it does confer the ability to survive under conditions of severe nutrient deprivation. We hypothesize that the Bvg+ phase is necessary and sufficient for respiratory tract colonization and the Bvg- phase is adapted for survival in environments encountered during transmission between hosts.
Topics: Animals; Bordetella; Humans; Phenotype; Phosphorylation; Respiratory System; Signal Transduction; Virulence
PubMed: 8876534
DOI: 10.1164/ajrccm/154.4_Pt_2.S150 -
Journal of Clinical Microbiology Feb 1978Clinical and bacteriological features of nine cases in which Achromobacter xylosoxidans were isolated in Hawaii are described. Five cases were ear infections mixed with...
Clinical and bacteriological features of nine cases in which Achromobacter xylosoxidans were isolated in Hawaii are described. Five cases were ear infections mixed with other gram-negative bacteria. Colonial morphology, xylose oxidation, peritrichous flagella staining, and antibiotic susceptibility pattern assisted in separating this bacterium from other nonfermentative, oxidase-positive, gram-negative rods.
Topics: Alcaligenes; Anti-Bacterial Agents; Bacterial Infections; Drug Resistance, Microbial; Hawaii; Humans; Otitis Externa
PubMed: 632351
DOI: 10.1128/jcm.7.2.239-241.1978 -
The Veterinary Record Mar 2022Taylorella equigenitalis has long been recognised as a causative agent of contagious equine metritis, but practitioners may be less familiar with Taylorella...
Taylorella equigenitalis has long been recognised as a causative agent of contagious equine metritis, but practitioners may be less familiar with Taylorella asinigenitalis, which has been identified more recently. Here, Abel Dorrego, Consuelo Serres and Fatima Cruz-Lopez of the Universidad Complutense de Madrid describe T asinigenitalis and report the findings of a survey they carried out in donkeys in Spain.
Topics: Animals; Equidae; Gram-Negative Bacterial Infections; Horse Diseases; Horses; Spain; Taylorella; Taylorella equigenitalis
PubMed: 35303356
DOI: 10.1002/vetr.1602 -
International Journal of Systematic and... May 2020Two Gram-stain-negative, short rod-shaped and non-flagellated strains, designated 17-4A and L52-1-41, were isolated from the surface seawater of the Indian Ocean and...
Two Gram-stain-negative, short rod-shaped and non-flagellated strains, designated 17-4A and L52-1-41, were isolated from the surface seawater of the Indian Ocean and South China Sea, respectively. The 16S rRNA genes of the two strains shared sequence similarity of 99.45 %. Strain 17-4A shared the highest 16S rRNA gene similarity of 98.02 % with EBR-8-1, followed by BN9 (97.47 %), MJ07 (96.93 %), Ch07 (96.68 %), DCY25 (96.65 %), PB3-7B (96.63 %), 24 (96.34 %), 54Pin (96.28 %) and B201 (96.05 %). L52-1-41 shared the highest 16S rRNA gene similarity of 97.74 % with EBR-8-1, followed by BN9 (97.47 %), MJ07 (96.65 %), Ch07 (96.41 %), DCY25 (96.37 %), PB3-7B (96.35 %), B201 (96.28 %), and 24 (96.06 %). The results of phylogenetic analyses indicated that 17-4A and L52-1-41 formed a stable, distinct and highly supported lineage affiliated to the genus . The results of the digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses indicated that they represented a single species. They featured similar genomic DNA G+C contents of 53.2-53.4 mol%. Activities of catalase and oxidase were negative for both strains. The fatty acids patterns of 17-4A and L52-1-41 were most similar, mostly comprised of C, Ccyclo, C, Cω9 and summed feature 8 (Cω7 and/or C ω6). The major polar lipids of the two strains were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The respiratory quinone of the two strains was Q-8. Hence, on the basis of the phenotypic, chemotaxonomic and genotypic data presented in this study, we proposed the classification of both strains as representatives of a novel species named sp. nov., with the type strain 17-4A (=MCCC 1A12670=KCTC 62121=NBRC 113794), and another strain L52-1-41 (=MCCC 1A05046=KCTC 52313).
Topics: Alcaligenaceae; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Indian Ocean; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Ubiquinone
PubMed: 32369004
DOI: 10.1099/ijsem.0.004202