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American Journal of Clinical Pathology Jul 1973
Topics: Alpharetrovirus; Animals; Cell Line; Cell Transformation, Neoplastic; Centrifugation, Density Gradient; DNA Nucleotidyltransferases; Fibrosarcoma; Gammaretrovirus; Giant Cell Tumors; Humans; Leukemia; Leukemia, Lymphoid; Oncogenic Viruses; RNA Viruses; RNA, Viral
PubMed: 4352547
DOI: 10.1093/ajcp/60.1.88 -
Harvey Lectures
Review
Topics: Alpharetrovirus; Animals; Antibodies, Viral; Avian Leukosis Virus; Biological Evolution; Birds; DNA Nucleotidyltransferases; DNA Replication; DNA, Viral; Epitopes; Genes; Genotype; Hybridization, Genetic; Neutralization Tests; Oncogenic Viruses; RNA Viruses; RNA, Viral; Reticuloendotheliosis virus; Virus Replication
PubMed: 4142634
DOI: No ID Found -
Cell Jan 1983Mutants of avian erythroblastosis virus (AEV) were constructed by deleting large nucleotide segments in each of the viral oncogenes termed v-erbA and v-erbB. Mutants in...
Mutants of avian erythroblastosis virus (AEV) were constructed by deleting large nucleotide segments in each of the viral oncogenes termed v-erbA and v-erbB. Mutants in erbA (erbA -B +) retained the ability to transform fibroblasts in vitro, and these cells exhibited most of the transformation characteristics that typify wild-type AEV-transformed fibroblasts. In addition, the mutants induced small erythroid colonies upon infection of bone marrow cells in culture. Chickens inoculated with erbA -B + virus or with erbA -B +-transformed cells developed sarcomas or atypical erythroid leukemias. The erythroid cells transformed in vivo or in vitro by the erbA -B + viruses appeared not to be as tightly blocked in differentiation as wild-type transformed cells. In contrast, fibroblasts infected with the erbA +B - mutant resembled normal cells in all transformation parameters tested, and no bone marrow cell transformation was observed with the mutant. The results indicate that the main transforming properties of AEV are encoded in erbB and that its effects are enhanced by erbA.
Topics: Alpharetrovirus; Avian Leukosis Virus; Bone Marrow Cells; Cell Transformation, Viral; Cells, Cultured; Chromosome Deletion; DNA, Viral; Erythroblasts; Fibroblasts; Mutation; Oncogenes; RNA, Viral; Viral Proteins
PubMed: 6297784
DOI: 10.1016/0092-8674(83)90513-5 -
Virology Feb 1972
Topics: Alpharetrovirus; Ammonium Sulfate; Animals; Carbon Isotopes; Centrifugation, Density Gradient; Chemical Precipitation; Culture Techniques; Ducks; Electrophoresis, Disc; Fibroblasts; Glucosamine; Glycoproteins; Herpesviridae; Microscopy, Electron; Pseudorabies; Simplexvirus; Sodium; Species Specificity; Sucrose; Sulfates; Thymidine; Tritium; Viral Proteins; Virus Cultivation
PubMed: 4333738
DOI: 10.1016/0042-6822(72)90279-6 -
Cell Nov 1996Viral envelope (Env)-receptor interactions have been implicated in the cell death associated with infection by subgroups B and D avian leukosis-sarcoma viruses (ALVs). A...
Viral envelope (Env)-receptor interactions have been implicated in the cell death associated with infection by subgroups B and D avian leukosis-sarcoma viruses (ALVs). A chicken protein, CAR1, was identified that permitted infection of mammalian cells by these viral subgroups. CAR1 bound to a viral Env fusion protein, comprising an ALV-B surface Env protein and the Fc region of an immunoglobulin, indicating that it is a specific viral receptor. CAR1 contains two extracellular cysteine-rich domains characteristic of the TNFR family and a cytoplasmic region strikingly similar to the death domain of TNFR1 and Fas, implicating this receptor in cell killing. Chicken embryo fibroblasts susceptible to ALV-B infection and transfected quail QT6 cells expressing CAR1 underwent apoptosis in response to the Env-Ig fusion protein, demonstrating that this cytopathic ALV receptor can mediate cell death.
Topics: 3T3 Cells; Alpharetrovirus; Animals; Apoptosis; Arginase; Base Sequence; COS Cells; Cloning, Molecular; DNA, Complementary; Fungal Proteins; Genes, Viral; Genome; Humans; Membrane Proteins; Mice; Molecular Sequence Data; Protein Binding; Quail; Receptors, Tumor Necrosis Factor; Sequence Homology, Amino Acid
PubMed: 8945512
DOI: 10.1016/s0092-8674(00)81992-3 -
Nature Mar 1976
Comparative Study
Topics: Alpharetrovirus; Animals; Avian Sarcoma Viruses; Biological Evolution; Birds; Cell Transformation, Neoplastic; Chickens; DNA; DNA, Viral; Ducks; Genes, Dominant; Nucleic Acid Denaturation; Nucleic Acid Renaturation; Quail
PubMed: 176594
DOI: 10.1038/260170a0 -
Proceedings of the National Academy of... Jul 1989The cloning and sequencing of the oncogene of the avian erythroblastosis virus S13 is described. The oncogene, termed v-sea, was found to be another member of the... (Comparative Study)
Comparative Study
The cloning and sequencing of the oncogene of the avian erythroblastosis virus S13 is described. The oncogene, termed v-sea, was found to be another member of the protein-tyrosine kinase gene family. The oncogene was fused in frame with the retrovirus S13 envelope gene, thus generating a fusion protein with a structure resembling that of a growth factor receptor. Sequence comparisons revealed that the v-sea gene was most closely related to the insulin receptor family of protein-tyrosine kinases, the greatest similarity being with the human MET oncogene.
Topics: Alpharetrovirus; Amino Acid Sequence; Animals; Avian Leukosis Virus; Base Sequence; Cloning, Molecular; Fibroblasts; Genes; Molecular Sequence Data; Multigene Family; Oncogene Proteins, Viral; Oncogenes; Protein-Tyrosine Kinases; Rats; Restriction Mapping; Sequence Homology, Nucleic Acid
PubMed: 2546151
DOI: 10.1073/pnas.86.14.5291 -
Journal of Virology May 1974The intracellular synthesis and integration of viral DNA (vDNA) into the host cell genome was studied in cultured chicken embryo fibroblasts infected with avian sarcoma...
The intracellular synthesis and integration of viral DNA (vDNA) into the host cell genome was studied in cultured chicken embryo fibroblasts infected with avian sarcoma or leukemia viruses. The newly synthesized vDNA was detected by hybridization with 70S viral RNA. Extraction of infected cell DNA by the selective procedure of Hirt resulted in the enrichment of newly synthesized vDNA in the low molecular weight supernatant fraction while leaving the bulk of cellular DNA containing integrated vDNA in the high molecular weight pellet fraction. This approach led to detection of intracellular vDNA synthesis within 1 h after infection and to vDNA integration into cellular DNA within 24 h. There was a several-fold increase in the vDNA content of infected cells during the initial phase of virus infection. But only a part of this newly synthesized vDNA appeared to become covalently linked with high molecular weight cellular DNA. Most of the remaining unintegrated vDNA gradually disappeared. The sedimentation profiles of minimally sheared cellular DNA in alkaline sucrose velocity gradients suggest that vDNA is synthesized as free linear molecules of approximately 3 x 10(6) daltons which subsequently are covalently linked to host cell DNA.
Topics: Alpharetrovirus; Animals; Avian Leukosis Virus; Avian Sarcoma Viruses; Centrifugation, Density Gradient; Chick Embryo; DNA, Viral; Fibroblasts; Nucleic Acid Hybridization; Time Factors
PubMed: 4363246
DOI: 10.1128/JVI.13.5.1005-1013.1974 -
Virus Research Jul 2008During retroviral integration, the viral integrase recognizes the attachment (att) sequence (formed by juxtaposition of two LTRs ends) as the substrate of integration....
During retroviral integration, the viral integrase recognizes the attachment (att) sequence (formed by juxtaposition of two LTRs ends) as the substrate of integration. We have developed a self-deleting Avian Leukosis and Sarcoma Viruses (ALSVs)-based retroviral vector carrying an additional copy of the att sequence, between neo and puro genes. We observed that: (i) the resulting NP3Catt vector was produced at neo and puro titers respectively smaller and higher than that of the parental vector devoid of the att sequence; (ii) 61% of NP3Catt proviruses were flanked by LTRs; most of them were deleted of internal sequences, probably during the reverse transcription step; (iii) 31% of clones were deleted of the whole 5' part of their genome and were flanked, in 5', by the additional att sequence and, in 3', by an LTR. Integration of these last proviruses was often imprecise with respect to the viral ends. At total, 77% of proviruses had lost the packaging signal and were not mobilizable by a replication-competent virus and 92% had lost the selectable gene in a single round of replication. Although still to improve, the att vector could be considered as an interesting new safe retroviral vector for gene transfer experiments.
Topics: Alpharetrovirus; Animals; Base Sequence; Cell Line; Gene Transfer Techniques; Genetic Vectors; Integrases; Proviruses; Quail; RNA, Viral; Sequence Deletion; Terminal Repeat Sequences; Viral Proteins; Virus Integration; Virus Replication
PubMed: 18420298
DOI: 10.1016/j.virusres.2008.02.009 -
Proceedings of the Society For... Oct 1973
Topics: Alpharetrovirus; Animals; Antibodies, Viral; Birds; Chickens; Humans; Kenya; Neutralization Tests; Species Specificity
PubMed: 4358887
DOI: 10.3181/00379727-144-37513