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Nihon Rinsho. Japanese Journal of... Aug 2005
Review
Topics: Adrenal Hyperplasia, Congenital; Amenorrhea; Androsterone; Biomarkers; Female; Humans; Hyperthyroidism; Hypertrichosis; Hypothyroidism; Male; Polycystic Ovary Syndrome; Radioimmunoassay; Reference Values; Specimen Handling
PubMed: 16149527
DOI: No ID Found -
Nihon Rinsho. Japanese Journal of... Dec 1999
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Nihon Rinsho. Japanese Journal of... Mar 1995
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The Journal of Physical Chemistry... Dec 2022We report the investigation of the steroid hormone androsterone in the gas phase. Androsterone is a male sex steroid hormone, being the first steroid hormone from this...
We report the investigation of the steroid hormone androsterone in the gas phase. Androsterone is a male sex steroid hormone, being the first steroid hormone from this category isolated and discovered 90 years ago. Despite the chemical compositions of steroids being well-known since long ago, studying their structures in the gas phase is still a challenging task, and to date, just a handful of detailed experimental structures for steroids have been reported. The rotational spectrum of androsterone was recorded in the 2-8 GHz frequency region with a broadband chirped-pulse Fourier transform microwave spectrometer coupled with supersonic expansion. From the weak rotational spectrum, one conformer has been detected in the isolated and cold conditions of the molecular jet. The combination of the experimental results with quantum chemical calculations allowed us to unambiguously identify the conformation of androsterone in the gas phase.
Topics: Male; Humans; Androsterone; Molecular Conformation; Spectrum Analysis; Steroids; Microwaves
PubMed: 36525396
DOI: 10.1021/acs.jpclett.2c03203 -
The Journal of Clinical Endocrinology... Aug 1967
Topics: Aged; Androsterone; Carbon Isotopes; Chromatography; Female; Glucuronates; Humans; Hydroxysteroid Dehydrogenases; Middle Aged; Radioisotope Dilution Technique; Sulfates; Tritium
PubMed: 5340762
DOI: 10.1210/jcem-27-8-1208 -
Hormone and Metabolic Research =... Apr 1996Serum androsterone was measured by radioimmunoassay in 79 girls and 80 boys aged between six months to sixteen years. In boys, androsterone levels were found to be low...
Serum androsterone was measured by radioimmunoassay in 79 girls and 80 boys aged between six months to sixteen years. In boys, androsterone levels were found to be low until the age of 12 years and then rapidly increased between the ages of 12-13 years. This increase of serum androsterone in boys seems to be related to the rapid increase of testosterone. On the other hand, in girls the androsterone levels were found to be low until the age of 10 years. However, from this age onwards, although individual values were variable, androsterone levels did not increase as rapidly as in boys but rather gradually with age which appeared to be influenced by the progressive increase of dehydroepiandrosterone (DHEA) in girls.
Topics: Adolescent; Aging; Androsterone; Child; Child, Preschool; Female; Humans; Infant; Male; Radioimmunoassay; Reference Values; Sex Characteristics
PubMed: 8740195
DOI: 10.1055/s-2007-979158 -
The Journal of Steroid Biochemistry and... Jun 2021A new androsterone derivative bearing a 16β-picolyl group (compound 5; FCO-586-119) was synthetized in four steps from the lead compound 1 (RM-532-105). We measured its...
16-Picolyl-androsterone derivative exhibits potent 17β-HSD3 inhibitory activity, improved metabolic stability and cytotoxic effect on various cancer cells: Synthesis, homology modeling and docking studies.
A new androsterone derivative bearing a 16β-picolyl group (compound 5; FCO-586-119) was synthetized in four steps from the lead compound 1 (RM-532-105). We measured its inhibitory activity on 17β-HSD3 using microsomal fraction of rat testes as well as transfected LNCaP[17β-HSD3] cells. We then assessed its metabolic stability as well as its cytotoxic effect against a panel of cancer cell lines. The addition of a picolyl moiety at C-16 of RM-532-105 steroid core improves the 17β-HSD3 inhibitory activity in the microsomal fraction of rat testes, but not in whole LNCaP[17β-HSD3] cells. Interestingly, this structural modification enhances 3-fold the metabolic stability in conjunction with a significant cytotoxic effect against pancreatic, ovarian, breast, lung, and prostate cancer cells. Because the inhibitory activity data against 17β-HSD3 suggested that both steroid derivatives are non-competitive inhibitors, we performed docking and molecular dynamics simulations using a homology model of this membrane-associated enzyme. The results of these simulations revealed that both RM-532-105 (1) and FCO-586-119 (5) can compete for the cofactor-binding site displaying better binding energy than NADP.
Topics: 17-Hydroxysteroid Dehydrogenases; Androstanes; Androsterone; Animals; Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Drug Stability; Enzyme Inhibitors; Humans; Magnetic Resonance Spectroscopy; Male; Models, Molecular; Molecular Docking Simulation; Molecular Dynamics Simulation; Molecular Structure; Prostatic Neoplasms; Rats, Sprague-Dawley; Sulfonamides; Rats
PubMed: 33609690
DOI: 10.1016/j.jsbmb.2021.105846 -
Molecules (Basel, Switzerland) Mar 2023Two series of novel steroidal[17,16-]pyrimidines derived from natural epiandrosterone and androsterone were designed and synthesized, and these compounds were screened...
Two series of novel steroidal[17,16-]pyrimidines derived from natural epiandrosterone and androsterone were designed and synthesized, and these compounds were screened for their potential anticancer activities. The preliminary bioassay indicated that some of these prepared compounds exhibited significantly good cytotoxic activities against human gastric cancer (SGC-7901), lung cancer (A549), and hepatocellular liver carcinoma (HepG2) cell lines compared with 5-fluorouracil (5-FU), epiandrosterone, and androsterone. Especially the respective pairs from epiandrosterone and androsterone showed significantly different inhibitory activities, and the possible configuration-activity relationships have also been summarized and discussed based on kinase assay and molecular docking, which indicated that the inhibition activities of these steroidal[17,16-]pyrimidines might obviously be affected by the configuration of the hydroxyl group in the part of the steroidal scaffold.
Topics: Humans; Androsterone; Pyrimidines; Molecular Docking Simulation; Cell Proliferation; Cell Line, Tumor; Antineoplastic Agents; Steroids; Fluorouracil; Carcinoma, Hepatocellular; Drug Screening Assays, Antitumor; Liver Neoplasms; Structure-Activity Relationship
PubMed: 36985662
DOI: 10.3390/molecules28062691 -
Nihon Rinsho. Japanese Journal of... Jul 2010
Topics: Adolescent; Adult; Androsterone; Child; Etiocholanolone; Female; Humans; Male; Middle Aged; Radioimmunoassay
PubMed: 20960798
DOI: No ID Found -
Hoppe-Seyler's Zeitschrift Fur... Mar 1961
Topics: 17-Ketosteroids; Adjuvants, Immunologic; Androsterone; Dinitrobenzenes
PubMed: 13728562
DOI: 10.1515/bchm2.1961.323.1.116