-
Il Farmaco; Edizione Pratica Oct 1959
Topics: Chymotrypsin; Hematologic Tests
PubMed: 13848946
DOI: No ID Found -
Proceedings of the National Academy of... Dec 1961
Topics: Chymotrypsin
PubMed: 13873928
DOI: 10.1073/pnas.47.12.1924 -
Archives of Ophthalmology (Chicago,... Jan 1961
Topics: Chymotrypsin
PubMed: 13783478
DOI: 10.1001/archopht.1961.01840020026006 -
Canadian Medical Association Journal Apr 1960
Topics: Cataract; Cataract Extraction; Chymotrypsin; Hematologic Tests; Lens, Crystalline
PubMed: 14436866
DOI: No ID Found -
Biochimica Et Biophysica Acta Jan 1960
Topics: Animals; Ascaris; Chymotrypsin; Protease Inhibitors
PubMed: 14431211
DOI: 10.1016/0006-3002(60)90101-3 -
The Biochemical Journal Jul 19681. The rates of deacylation of acyl-alpha-chymotrypsins in which the hydrogen-bonding capacity of the acylamino group of the substrate has been systematically removed...
1. The rates of deacylation of acyl-alpha-chymotrypsins in which the hydrogen-bonding capacity of the acylamino group of the substrate has been systematically removed were measured. 2. The ratio of deacylation rates of l- and d-acyl-enzymes is found to depend largely on the existence in the substrate of an amido -NH- group. 3. The data presented agree with the postulate that the stereospecificity of alpha-chymotrypsin is exercised in catalytic rather than binding steps, and that the active site of the enzyme presents three loci to the substrate: the site containing the catalytic functionalities (including serine-195), the hydrophobic area for amino acid side-chain binding, and a hydrogen-bond acceptor site for acylamino group binding. 4. It is noted that, though the hydrogen-bonding site is crucial for the stereospecificity, the free energy of binding of substrates and inhibitors is dominated by the hydrophobic interaction. 5. It is tentatively proposed that alpha-chymotrypsin selects a high-energy conformation of the substrate when the latter binds at the enzyme's active site.
Topics: Amino Acids; Binding Sites; Chemical Phenomena; Chemistry; Chymotrypsin; Hydrogen-Ion Concentration; Kinetics; Stereoisomerism
PubMed: 5667268
DOI: 10.1042/bj1080561 -
Bulletin of the New York Academy of... Jan 1961
Topics: Cataract; Cataract Extraction; Chymotrypsin; Hematologic Tests; Lens, Crystalline
PubMed: 13758376
DOI: No ID Found -
British Medical Journal Dec 1960
Topics: Cataract; Cataract Extraction; Chymotrypsin; Hematologic Tests; Humans; Lens, Crystalline
PubMed: 13735589
DOI: 10.1136/bmj.2.5213.1629 -
British Medical Journal Aug 1959
Topics: Cataract Extraction; Chymotrypsin; Hematologic Tests; Humans
PubMed: 13671226
DOI: No ID Found -
Canadian Medical Association Journal Apr 1960
Topics: Cataract; Cataract Extraction; Chymotrypsin; Hematologic Tests; Lens, Crystalline
PubMed: 14447002
DOI: No ID Found