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Annals of the New York Academy of... Oct 1960
Topics: Dactinomycin; Humans
PubMed: 13782613
DOI: 10.1111/j.1749-6632.1960.tb20172.x -
Journal of Natural Products Aug 2006Five new members of the actinomycin family, actinomycins G2-G6 (2-6), are produced by Streptomyces iakyrus strain DSM 41873. Their structures were established by...
Five new members of the actinomycin family, actinomycins G2-G6 (2-6), are produced by Streptomyces iakyrus strain DSM 41873. Their structures were established by spectroscopic methods. Unlike actinomycin D (1), the alpha-ring of the novel compounds contains the unusual amino acid 3-hydroxy-5-methylproline, while the beta-ring includes N-methylalanine and either a chlorinated or hydroxylated threonine moiety. The chlorine-containing actinomycin G2 (2) is the main product; it exhibits strong cytotoxic and antibacterial activities. Actinomycin G5 (5) is the first actinomycin with an additional ring closure between the beta-peptidolactone and the actinoyl chromophore. Actinomycin G6 (6) resulted from the 4-hydroxythreonine-containing actinomycin G3 (3) by a 2-fold acyl shift of the beta-unit, which has not been observed before for this class of chromopeptides. The structural modification of compounds 5 and 6 goes along with an evident reduction of the biological activity. The biosynthesis of aniso-actinomycins is discussed.
Topics: Bacillus subtilis; Candida albicans; Citrus sinensis; Dactinomycin; Drug Screening Assays, Antitumor; Escherichia coli; Humans; Israel; Lactones; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Staphylococcus aureus; Stereoisomerism; Streptomyces; Structure-Activity Relationship; Threonine; Tumor Cells, Cultured
PubMed: 16933866
DOI: 10.1021/np060063g -
Journal of Gynecologic Oncology Jul 2019
Topics: Antibiotics, Antineoplastic; Brazil; Dactinomycin; Drug Industry; Female; Gestational Trophoblastic Disease; Humans; Pregnancy
PubMed: 31074249
DOI: 10.3802/jgo.2019.30.e87 -
Methods in Enzymology 1995The application of high-resolution, multidimensional NMR techniques to the problem of determining the structure of drug-DNA complexes in solution has led to substantial...
The application of high-resolution, multidimensional NMR techniques to the problem of determining the structure of drug-DNA complexes in solution has led to substantial progress in understanding the effect of drugs on DNA at the molecular level. With the development of isotopic labeling methods applied in three- and four-dimensional experiments, we anticipate that more complex drug-DNA systems will become amenable to structural analysis. In addition to implementing these newer techniques, progress will also be made in terms of investigating the structure of drug complexes with more unusual forms of DNA, such as triplexes, quadruplexes, multistranded junctions, and so forth.
Topics: Algorithms; Binding Sites; DNA; Dactinomycin; Hydrogen-Ion Concentration; Intercalating Agents; Isotopes; Magnetic Resonance Spectroscopy; Molecular Weight; Pharmaceutical Preparations; Plicamycin; Protons; Software; Solubility
PubMed: 8569513
DOI: 10.1016/s0076-6879(95)61026-x -
Annals of the New York Academy of... Oct 1960
Topics: Animals; Dactinomycin; Leukemia; Leukemia, Experimental
PubMed: 13688927
DOI: 10.1111/j.1749-6632.1960.tb20163.x -
Nature Jun 1976
Topics: Biological Transport; Cell Line; Dactinomycin; Drug Resistance; Liposomes; RNA
PubMed: 934317
DOI: 10.1038/261699a0 -
Maandschrift Voor Kindergeneeskunde Feb 1971
Topics: Abdominal Neoplasms; Child; Dactinomycin; Diagnosis, Differential; Humans
PubMed: 5101104
DOI: No ID Found -
European Journal of Cancer Nov 1980
Comparative Study
Topics: Adsorption; Animals; Cyanoacrylates; Dactinomycin; Drug Administration Schedule; Male; Neoplasm Transplantation; Rats; Sarcoma, Experimental; Soft Tissue Neoplasms
PubMed: 7227421
DOI: 10.1016/0014-2964(80)90053-5 -
Bioorganic & Medicinal Chemistry Letters Aug 1998Actinomycin D, C2 and VII, cyclic peptides, inhibited Grb2 SH2 domain association (IC50 5-7 microM) with a phosphotyrosine containing peptide derived from the Shc...
Actinomycin D, C2 and VII, cyclic peptides, inhibited Grb2 SH2 domain association (IC50 5-7 microM) with a phosphotyrosine containing peptide derived from the Shc protein (pTyr317). Actinomycins are the first examples of nonphosphorylated natural ligands of SH2 domain.
Topics: Adaptor Proteins, Signal Transducing; Dactinomycin; GRB2 Adaptor Protein; Molecular Structure; Proteins; Spectrometry, Mass, Fast Atom Bombardment; Spectrophotometry, Ultraviolet; Streptomyces
PubMed: 9873474
DOI: 10.1016/s0960-894x(98)00345-x -
Bioorganic & Medicinal Chemistry Letters Jul 2000Natural analogues (D, C2, and VII) of actinomycin inhibit Grb2 SH2 domain binding with phosphopeptide-derived from Shc in vitro and in intracellular system. To study...
Natural analogues (D, C2, and VII) of actinomycin inhibit Grb2 SH2 domain binding with phosphopeptide-derived from Shc in vitro and in intracellular system. To study structure-activity relationships, 13 actinomycin analogues were synthesized and we found that the inhibition activity depended on the substituents of cyclic peptide groups in actinomycin and two analogues with Tyr residue are the most potent inhibitors with IC50 value of 0.5 and 0.8 microM, respectively.
Topics: Adaptor Proteins, Signal Transducing; Adaptor Proteins, Vesicular Transport; Amino Acid Substitution; Animals; Antibiotics, Antineoplastic; Biological Assay; Cell Line, Transformed; Dactinomycin; GRB2 Adaptor Protein; Growth Inhibitors; Humans; Immunoblotting; Molecular Structure; Precipitin Tests; Protein Binding; Proteins; Recombinant Fusion Proteins; Shc Signaling Adaptor Proteins; Src Homology 2 Domain-Containing, Transforming Protein 1; Structure-Activity Relationship; src Homology Domains
PubMed: 10888331
DOI: 10.1016/s0960-894x(00)00258-4