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Indian Journal of Pharmacology 2017The objective of the study was to investigate the effect of daidzein flavonoid on cisplatin (CP)-induced hematotoxicity and hepatotoxicity in experimental rats.
OBJECTIVE
The objective of the study was to investigate the effect of daidzein flavonoid on cisplatin (CP)-induced hematotoxicity and hepatotoxicity in experimental rats.
MATERIALS AND METHODS
The Wistar rats were randomly divided into four equal groups: Normal (saline 1 ml p.o.), CP (7.5 mg/kg once intraperioteneally on 16 day), test group of low dose (combination of CP and daidzein 20 mg/kg p.o. for 21 days), and test group of high dose (combination of CP and daidzein 40 mg/kg p.o. for 21 days). Blood samples were collected on 22 day from each rat and subjected for evaluation of hematological parameters such as red blood corpuscles (RBC), white blood corpuscles, hemoglobin (Hb) and platelets, and serum biomarkers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Liver of each rat was excised and subjected for antioxidants evaluation such as malonyl dialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase, and histopathological study.
RESULTS
Daidzein had a significant ( < 0.001) beneficial role in CP-induced hemotoxicity by increasing RBC, Hb, packed cell volume, and platelets. Daidzein also exhibited a significant ( < 0.001) protection against CP-induced hepatotoxicity by decreasing ALT, AST, ALP, and MDA level and by elevating the GSH, SOD, and catalase.
CONCLUSIONS
Daidzein attenuates CP-induced oxidative stress on blood cells and antioxidants in rats.
Topics: Alanine Transaminase; Animals; Antineoplastic Agents; Antioxidants; Aspartate Aminotransferases; Chemical and Drug Induced Liver Injury; Cisplatin; Dose-Response Relationship, Drug; Female; Glutathione; Isoflavones; Male; Oxidative Stress; Rats; Rats, Wistar; Superoxide Dismutase
PubMed: 28458422
DOI: 10.4103/0253-7613.201022 -
Food & Function Sep 2021soy protein and soy isoflavones have been suggested to be associated with improved cardiovascular risk factors (e.g., lipid profiles and uric acid (UA)), but few studies... (Randomized Controlled Trial)
Randomized Controlled Trial
Effects of daidzein and genistein on markers of cardiovascular disease risk among women with impaired glucose regulation: a double-blind, randomized, placebo-controlled trial.
BACKGROUND AND OBJECTIVE
soy protein and soy isoflavones have been suggested to be associated with improved cardiovascular risk factors (e.g., lipid profiles and uric acid (UA)), but few studies have been conducted among women with impaired glucose regulation (IGR). This study is aimed to evaluate the effect of isolated daidzein and genistein on lipid profiles, high sensitive C-reactive protein (hs-CRP), and uric acid (UA) among Chinese women with IGR.
METHODS AND RESULTS
this randomized, double-blind, and placebo-controlled trial was conducted in 165 Chinese women aged 30-70 years with IGR. Participants were randomly assigned to one of the three groups: 0 mg of daidzein and genistein with 10 g soy protein (placebo group), 50 mg of daidzein with 10 g soy protein (daidzein group), or 50 mg of genistein with 10 g soy protein (genistein group) supplementation for 24 weeks. Fasting serum total cholesterol (TC), triacylglycerol (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), lipoprotein a (LP (a)), hs-CRP, and UA were assessed at baseline, 12, and 24 weeks after intervention. The results showed no significant differences in the changes (%) of TC, TG, HDL-C, LDL-C, LP (a), hs-CRP, and UA between the three treatment groups at weeks 12 or 24 (all P > 0.05).
CONCLUSION
neither isolated daidzein nor genistein had a significant effect on cardiovascular health in Chinese women with IGR.
Topics: Adult; Aged; Biomarkers; C-Reactive Protein; Cardiovascular Diseases; Cholesterol, HDL; Cholesterol, LDL; Double-Blind Method; Female; Genistein; Glucose; Humans; Isoflavones; Middle Aged; Triglycerides
PubMed: 34263280
DOI: 10.1039/d1fo00712b -
BioFactors (Oxford, England) Jan 2021Phytoestrogens can control high-fat diet-induced hypothalamic inflammation that is associated with severe consequences, including obesity, type 2 diabetes,...
Phytoestrogens can control high-fat diet-induced hypothalamic inflammation that is associated with severe consequences, including obesity, type 2 diabetes, cardiovascular and neurodegenerative diseases. However, the phytoestrogen anti-neuroinflammatory action is poorly understood. In this study, we explored the neuroprotection mediated by daidzein in hypothalamic neurons by using a membrane-based model of obesity-related neuroinflammation. To test the daidzein therapeutic potential a biohybrid membrane system, consisting of hfHypo GnRH-neurons in culture on PLGA membranes, was set up. It served as reliable in vitro tool capable to recapitulate the in vivo structure and function of GnRH hypothalamic tissue. Our findings highlighted the neuroprotective role of daidzein, being able to counteract the palmitate induced neuroinflammation. Daidzein protected hfHypo GnRH cells by downregulating cell death, proinflammatory processes, oxidative stress, and apoptosis. It also restored the proper cell morphology and functionality through a mechanism which probably involves the activation of ERβ and GPR30 receptors along with the expression of GnRH peptide and KISS1R.
Topics: Anti-Inflammatory Agents; Antioxidants; Apoptosis; Cells, Cultured; Encephalitis; Gonadotropin-Releasing Hormone; Humans; Hypothalamus; Isoflavones; Membranes, Artificial; Models, Biological; Neurons; Neuroprotective Agents; Palmitates; Phytoestrogens; Polylactic Acid-Polyglycolic Acid Copolymer
PubMed: 33350001
DOI: 10.1002/biof.1701 -
Journal of Chromatography. A Nov 2009Daidzein-imprinted poly(methacrylamide-co-acrylic acid) composite membranes with different ratios of methacrylamide (MAM) versus acrylic acid (AA) were prepared via UV...
Daidzein-imprinted poly(methacrylamide-co-acrylic acid) composite membranes with different ratios of methacrylamide (MAM) versus acrylic acid (AA) were prepared via UV initiated photo-copolymerization on the commercial filter paper with ethylene glycol dimethacrylate (EGDMA) as cross-linker and mixed cellulose ester as agglutinant. Infra-red (IR) spectroscopy and scanning electron microscope (SEM) were used to visualize the surface of the membranes. Binding and recognising properties of the imprinted composite membranes to daidzein and its analogues genistein were evaluated by static adsorption experiment. It was found that the daidzein-imprinted membranes showed high selectivity to daidzein, with the highest selectivity when the composite membrane with the ratio of MAM vs AA as 4:1. The results suggested that the molecularly imprinted composite membranes were potentially useful for daidzein enrichment.
Topics: Acrylates; Adsorption; Isoflavones; Membranes, Artificial; Methacrylates; Molecular Imprinting; Photochemical Processes; Polymers; Polymethacrylic Acids; Ultraviolet Rays
PubMed: 19782989
DOI: 10.1016/j.chroma.2009.08.093 -
Oncology Reports Oct 2017Choriocarcinoma is a highly malignant tumor arising from abnormal gestational trophoblast proliferation. Although chemotherapy has dramatically improved the prognosis,...
Choriocarcinoma is a highly malignant tumor arising from abnormal gestational trophoblast proliferation. Although chemotherapy has dramatically improved the prognosis, there are still some patients who become drug-resistant or relapse. Daidzein has garnered interest in its antitumor activity especially in proliferation inhibition. However, few reports exist on daidzein effect in growth of choriocarcinoma. Therefore, in this study, we performed in vitro and in vivo experiment in JAR and JEG‑3 to investigate the effect of daidzein in proliferation of choriocarcinoma. Daidzein inhibited cell growth in a time- and dose-dependent way. Cell cycle was arrested at G1 phase and expression of cyclin D1, c-myc, PCNA was reduced while p21 was upregulated during daidzein treatment. At the same time, the expression of p-ERK was downregulated and translocation into nuclear afterwards was also inhibited. Moreover, ERK agonist ceramide C6 abolished daidzein's effects on cell proliferation. Besides, in vivo experiment also showed daidzein's anti-proliferation function as xenografts growth was inhibited and expressions of c-myc, PCNA and p-ERK were suppressed. In conclusion, results in our study demonstrate daidzein can inhibit choriocarcinoma cell proliferation in vitro and in vivo; underlying mechanism behind the inhibitory effects may probably be suppressing ERK pathway and afterwards arresting cell cycle at G1 phase.
Topics: Animals; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Proliferation; Choriocarcinoma; G1 Phase; Gene Expression Regulation, Neoplastic; Humans; Isoflavones; MAP Kinase Signaling System; Mice; Neoplasm Proteins; Neoplasm Recurrence, Local; Phosphorylation; Xenograft Model Antitumor Assays
PubMed: 28849226
DOI: 10.3892/or.2017.5928 -
Nutrition Research (New York, N.Y.) Jun 2017Daidzein, a natural soy isoflavone, has a structure similar to estradiol and exhibiting bone-sparing effects against osteoporosis. However, the molecular mechanisms of...
Daidzein stimulates osteogenesis facilitating proliferation, differentiation, and antiapoptosis in human osteoblast-like MG-63 cells via estrogen receptor-dependent MEK/ERK and PI3K/Akt activation.
Daidzein, a natural soy isoflavone, has a structure similar to estradiol and exhibiting bone-sparing effects against osteoporosis. However, the molecular mechanisms of osteogenesis remain unclear. We hypothesized that daidzein stimulates osteogenesis through estrogen receptor (ER)-dependent signal pathways. To test this hypothesis, we investigated the effects of daidzein compared with 17β-estradiol on proliferation, differentiation, and cisplatin-induced apoptosis in human osteoblast-like MG-63 cells containing 2 ER isoforms. The results showed that daidzein stimulated cell proliferation by altering cell cycle distribution, promoted cell differentiation by increasing the alkaline phosphatase activity and collagen content, and reduced cell apoptosis associated by up-regulating the expression of Bcl-xL. The above actions of daidzein were prevented by cotreatment with the ER antagonist ICI 182780. Using small interfering RNA technology, we further demonstrated that the effects of daidzein on alkaline phosphatase activity, collagen content, and cell apoptosis are mediated by both ERα and ERβ, whereas the effects on cell proliferation are primarily mediated by ERα. However, the effects of 17β-estradiol on osteoblastic proliferation and survival are mediated by both ER isotypes, and the effects on osteoblastic differentiation are primarily mediated by ERα. The use of specific inhibitors indicated that activation of the mitogen-activated protein kinase kinase/extracellular regulated kinase (MEK/ERK) and phosphoinositide 3-kinase/protein kinase B or PKB (PI3K/Akt) signaling pathway at least partially accounts for these effects of daidzein. Taken together, the results indicate that daidzein stimulates osteogenesis through facilitating proliferation, differentiation, and antiapoptosis in human osteoblast-like MG-63 cells via activation of MEK/ERK and PI3K/Akt in an ER-dependent manner.
Topics: Alkaline Phosphatase; Apoptosis; Cell Differentiation; Cell Line; Cell Proliferation; Cisplatin; Estradiol; Estrogen Receptor Antagonists; Fulvestrant; Humans; Isoflavones; Osteoblasts; Osteogenesis; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Receptors, Estrogen; Signal Transduction; bcl-X Protein
PubMed: 28633868
DOI: 10.1016/j.nutres.2017.04.009 -
Cell Biochemistry and Biophysics Mar 2013Daidzein belongs to the group of isoflavones, found in a wide variety of plant-derived foods, especially in soybeans and soy-based foods. In this study, the effect of...
Daidzein belongs to the group of isoflavones, found in a wide variety of plant-derived foods, especially in soybeans and soy-based foods. In this study, the effect of daidzein on human gastric carcinoma cells (BGC-823) and its mechanism were investigated. MTT assay was applied in the detection of the inhibitory effects of daidzein on cell proliferation. Hoechst-propidium iodide staining and flow cytometry were used to examine the apoptosis as well as the mitochondrial transmembrane potential. Western blotting was performed to detect the expression of apoptosis-associated proteins: cleaved PARP, cleaved caspase-9, cleaved caspase-3, Bcl-2, and Bax. Daidzein significantly inhibited the growth and proliferation of human gastric carcinoma cells (BGC-823) in a concentration- and time-dependent manner. Furthermore, it was found that an insult of daidzein to BGC-823 cells caused them to die by disruption of mitochondrial transmembrane potential, demonstrated not only by staining dead cells for phosphatidylserine but also by the up-regulation (cleaved PARP, cleaved caspase-9, cleaved caspase-3, Bax) and down-regulation (Bcl-2) of proteins associated with apoptosis and survival; whereas, the pan-caspase inhibitor z-VAD-fmk could partially rescue cells against damage of daidzein. Taken together, the results of this study demonstrate that daidzein significantly induces apoptosis via a mitochondrial pathway. Specifically, daidzein induced a change in the Bax/Bcl-2 ratios and activation of caspases-3 and -9 and the cleavage of PARP. Therefore, daidzein has the potential for use as a therapeutic agent for the treatment of gastric carcinoma.
Topics: Apoptosis; Blotting, Western; Caspase 3; Caspase 9; Cell Line, Tumor; Cell Proliferation; Dose-Response Relationship, Drug; Down-Regulation; Enzyme Activation; Flow Cytometry; Humans; Isoflavones; Membrane Potential, Mitochondrial; Mitochondria; Phytoestrogens; Poly(ADP-ribose) Polymerases; Proto-Oncogene Proteins c-bcl-2; Signal Transduction; Stomach Neoplasms; Time Factors; bcl-2-Associated X Protein
PubMed: 22926545
DOI: 10.1007/s12013-012-9418-2 -
Journal of Molecular Modeling Jun 2019Radix puerariae-a popular traditional Chinese medicine-is used for the treatment of diarrhea, acute dysentery, deafness, and cardiovascular diseases. It can also be used...
Radix puerariae-a popular traditional Chinese medicine-is used for the treatment of diarrhea, acute dysentery, deafness, and cardiovascular diseases. It can also be used as an effective antioxidant and has been tested as an anticancer drug. Daidzein and puerarin are its main active compounds. The present contribution was focused on experimental and theoretical studies of the H and C NMR chemical shifts and nuclear magnetic shielding parameters of daidzein and puerarin. Experimental data were gained by exploring standard one-dimensional spectra and a set of two-dimensional measurements: COSY, HSQC, and HMBC. The theoretical gauge independent atomic orbital density functional theory supporting studies, were performed to determine shielding constants and chemical shifts in vacuum and methanol-d4 solvent. The correlation between experimental and theoretical data was fairly good, especially when the DFT/PBE0 approach was used. The molecular properties of daidzein and puerine related to antiradical activity were studied in the context of a single-step hydrogen atom transfer mechanism and its correlation with C NMR chemical shifts. Graphical abstract Electron spin density distribution for daidzein radical.
Topics: Antioxidants; Asian People; China; Drugs, Chinese Herbal; Humans; Isoflavones; Medicine, Chinese Traditional; Nuclear Magnetic Resonance, Biomolecular
PubMed: 31243583
DOI: 10.1007/s00894-019-4090-8 -
Nanoscale Apr 2011A nano-based delivery system was developed to improve the oral absorption of daidzein, which has poor hydrophilicity and lipophilicity. A daidzein-phospholipid complex...
A nano-based delivery system was developed to improve the oral absorption of daidzein, which has poor hydrophilicity and lipophilicity. A daidzein-phospholipid complex (DPC) was firstly prepared to improve its lipophilicity, and then encapsulated into lipid nanocarriers (DLNs) to verify the effectiveness of the strategy in enhancing the oral delivery of daidzein. DLNs were spherical nanosized particles with evidently increased dissolution. DLNs were mainly distributed in stomach and proximal intestine of mice after oral administration, and the intestinal permeability of DLNs in rats was significantly improved when compared with that of daidzein solution. The peak concentration of daidzein in rats after oral administration of DPC and DLNs was 6833 ± 1112 ng mL(-1) and 14,512 ± 2390 ng mL(-1), respectively, which was improved over 10-fold and 21-fold than that of free daidzein. Moreover, the areas under the concentration-time curve (AUC(0-t)) of DPC and DLNs were enhanced by 3.62-fold and 6.87-fold compared with that of free daidzein. These results suggested that DLNs could be an effective strategy to improve the oral absorption of poor hydrophilic and lipophilic drugs like daidzein.
Topics: Administration, Oral; Animals; Gastric Mucosa; Intestinal Mucosa; Isoflavones; Metabolic Clearance Rate; Mice; Nanocapsules; Organ Specificity; Phospholipids; Rats; Tissue Distribution
PubMed: 21350765
DOI: 10.1039/c0nr00879f -
Annals of Oncology : Official Journal... Feb 2010In order to study the anticancer effects and cellular apoptosis pathways induced by daidzein.
BACKGROUND
In order to study the anticancer effects and cellular apoptosis pathways induced by daidzein.
MATERIALS AND METHODS
We used the human MCF-7 breast cancer cell line as a model and examined the apoptosis by Hoechst-propidium iodide staining fluorescence imaging and flow cytometry.
RESULTS
Our data indicated that daidzein induces antiproliferative effects in a concentration- and time-dependent manner. We demonstrated that daidzein-induced apoptosis in MCF-7 cells was initiated by the generation of reactive oxygen species (ROS). Furthermore, we showed that this daidzein-induced ROS generation was accompanied by disruption of mitochondrial transmembrane potential, down-regulation of bcl-2, and up-regulation of bax, which led to the release of cytochrome C from the mitochondria into the cytosol, which, in turn, resulted in the activation of caspase-9 and caspase-7, and ultimately in cell death. The induction of the mitochondrial caspase-dependent pathway was confirmed by pretreatment with pan-caspase inhibitor z-VAD-fmk and antioxidant N-acetyl-L-cysteine.
CONCLUSION
Accordingly, daidzein could induce breast cancer cell apoptosis through the mitochondrial caspase-dependent cell death pathway.
Topics: Antineoplastic Agents, Phytogenic; Apoptosis; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Female; Humans; Isoflavones; Membrane Potential, Mitochondrial; Mitochondria; Phytoestrogens; Reactive Oxygen Species; Signal Transduction; Time Factors; Up-Regulation
PubMed: 19889614
DOI: 10.1093/annonc/mdp499